Streptococcus satellite phage Javan19
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFD5|A0A4D5ZFD5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan19 OX=2558557 GN=JavanS19_0016 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 TFSDD6 pKa = 3.67 TPKK9 pKa = 10.21 TFTFHH14 pKa = 5.7 YY15 pKa = 8.55 TFKK18 pKa = 11.1 DD19 pKa = 3.09 FDD21 pKa = 4.09 TAQVACHH28 pKa = 6.88 AILGYY33 pKa = 7.77 MTGTYY38 pKa = 7.11 EE39 pKa = 4.76 QPVIDD44 pKa = 3.97 ATYY47 pKa = 10.82 HH48 pKa = 6.24 NDD50 pKa = 3.59 DD51 pKa = 3.72 QGGHH55 pKa = 6.63 ANQLVLKK62 pKa = 9.52 YY63 pKa = 10.97 AEE65 pKa = 4.31 DD66 pKa = 3.69 RR67 pKa = 11.84 KK68 pKa = 10.48 LSKK71 pKa = 10.32 VFKK74 pKa = 10.15 RR75 pKa = 11.84 ICDD78 pKa = 3.69 SFKK81 pKa = 10.93 DD82 pKa = 4.21 YY83 pKa = 11.52 YY84 pKa = 10.51 NQPEE88 pKa = 4.31 DD89 pKa = 3.62 MTDD92 pKa = 3.31 EE93 pKa = 4.68 EE94 pKa = 5.58 LDD96 pKa = 4.63 DD97 pKa = 3.7 MAQEE101 pKa = 4.07 NEE103 pKa = 4.3 LIKK106 pKa = 10.55 EE107 pKa = 4.12 VEE109 pKa = 4.12 EE110 pKa = 5.42 LDD112 pKa = 3.9 DD113 pKa = 3.76 QRR115 pKa = 11.84 VVPLFEE121 pKa = 4.1 ITQEE125 pKa = 4.02 EE126 pKa = 4.66 ANEE129 pKa = 4.0 QDD131 pKa = 3.13 TLMAFISDD139 pKa = 3.57 HH140 pKa = 6.56 DD141 pKa = 3.74 QLAEE145 pKa = 4.12 HH146 pKa = 7.09 LSMNYY151 pKa = 9.71 QEE153 pKa = 4.96 MSQDD157 pKa = 3.43 DD158 pKa = 4.03 LGAILEE164 pKa = 4.7 TISQAFNHH172 pKa = 6.79 LYY174 pKa = 11.22 DD175 pKa = 3.83 MVVEE179 pKa = 4.32 GQLLVKK185 pKa = 10.69
Molecular weight: 21.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.122
IPC2_protein 4.164
IPC_protein 4.139
Toseland 3.948
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.253
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.859
Solomon 4.101
Lehninger 4.062
Nozaki 4.215
DTASelect 4.418
Thurlkill 3.973
EMBOSS 4.024
Sillero 4.253
Patrickios 3.109
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.158
Protein with the highest isoelectric point:
>tr|A0A4D5ZKZ7|A0A4D5ZKZ7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan19 OX=2558557 GN=JavanS19_0001 PE=4 SV=1
MM1 pKa = 7.38 IMKK4 pKa = 8.9 ITEE7 pKa = 4.08 VKK9 pKa = 10.56 KK10 pKa = 10.72 KK11 pKa = 10.15 DD12 pKa = 3.14 GTVIYY17 pKa = 9.92 RR18 pKa = 11.84 ASIYY22 pKa = 10.71 LGTDD26 pKa = 2.36 KK27 pKa = 10.8 VTGKK31 pKa = 10.64 KK32 pKa = 7.91 VTTKK36 pKa = 9.18 ITGRR40 pKa = 11.84 TKK42 pKa = 10.6 KK43 pKa = 9.66 EE44 pKa = 3.56 VRR46 pKa = 11.84 EE47 pKa = 3.99 KK48 pKa = 11.0 AKK50 pKa = 10.9 YY51 pKa = 9.16 EE52 pKa = 3.66 AVEE55 pKa = 4.67 FIKK58 pKa = 11.11 NGSTRR63 pKa = 11.84 FKK65 pKa = 9.21 ATSVTSYY72 pKa = 11.34 QEE74 pKa = 4.91 LATLWWDD81 pKa = 3.74 SYY83 pKa = 11.1 KK84 pKa = 9.85 HH85 pKa = 4.21 TVKK88 pKa = 10.92 YY89 pKa = 8.4 NTQLATEE96 pKa = 4.37 KK97 pKa = 10.61 LLTVHH102 pKa = 6.77 VIPIFGAYY110 pKa = 9.53 KK111 pKa = 10.15 LDD113 pKa = 3.67 KK114 pKa = 10.12 LTTPLIQSIINKK126 pKa = 9.54 LADD129 pKa = 3.45 KK130 pKa = 9.75 TNKK133 pKa = 10.06 GEE135 pKa = 4.09 RR136 pKa = 11.84 KK137 pKa = 9.79 AYY139 pKa = 9.6 LHH141 pKa = 6.59 YY142 pKa = 11.09 DD143 pKa = 4.1 RR144 pKa = 11.84 IHH146 pKa = 6.67 ALNKK150 pKa = 10.16 RR151 pKa = 11.84 ILQYY155 pKa = 10.96 GVIMQAIPFNPARR168 pKa = 11.84 EE169 pKa = 4.24 VILPRR174 pKa = 11.84 NTKK177 pKa = 9.3 KK178 pKa = 10.99 ANTKK182 pKa = 9.27 RR183 pKa = 11.84 VKK185 pKa = 10.5 HH186 pKa = 5.63 FEE188 pKa = 3.77 NDD190 pKa = 3.02 EE191 pKa = 3.81 LRR193 pKa = 11.84 TFFNYY198 pKa = 10.41 LNNLDD203 pKa = 3.7 KK204 pKa = 11.1 SKK206 pKa = 10.86 YY207 pKa = 8.12 RR208 pKa = 11.84 YY209 pKa = 9.24 FYY211 pKa = 10.89 EE212 pKa = 3.82 VTLYY216 pKa = 11.0 KK217 pKa = 10.48 FLLATGCRR225 pKa = 11.84 INEE228 pKa = 3.8 ALALNWSDD236 pKa = 3.65 IDD238 pKa = 3.87 LDD240 pKa = 3.8 NAVVHH245 pKa = 5.12 VTKK248 pKa = 10.15 TLNYY252 pKa = 8.35 KK253 pKa = 9.4 QEE255 pKa = 4.1 INSPKK260 pKa = 10.23 SKK262 pKa = 10.48 SSYY265 pKa = 10.17 RR266 pKa = 11.84 DD267 pKa = 2.69 IDD269 pKa = 3.27 IDD271 pKa = 3.87 SRR273 pKa = 11.84 TVTMLKK279 pKa = 9.73 QYY281 pKa = 10.25 RR282 pKa = 11.84 RR283 pKa = 11.84 RR284 pKa = 11.84 QIQEE288 pKa = 2.96 AWKK291 pKa = 9.76 LGRR294 pKa = 11.84 SEE296 pKa = 4.29 TVVFSDD302 pKa = 6.41 FIHH305 pKa = 7.11 KK306 pKa = 10.24 YY307 pKa = 8.57 PNNRR311 pKa = 11.84 TLQTRR316 pKa = 11.84 LRR318 pKa = 11.84 THH320 pKa = 6.83 FKK322 pKa = 10.02 RR323 pKa = 11.84 ANVSNIGFHH332 pKa = 6.27 GFRR335 pKa = 11.84 HH336 pKa = 4.87 THH338 pKa = 6.65 ASLLLNTGIPYY349 pKa = 10.09 KK350 pKa = 10.14 EE351 pKa = 3.64 LQYY354 pKa = 11.41 RR355 pKa = 11.84 LGHH358 pKa = 5.67 STLSMTMDD366 pKa = 3.58 IYY368 pKa = 11.68 SHH370 pKa = 6.98 LSKK373 pKa = 11.0 EE374 pKa = 4.25 NAKK377 pKa = 10.34 KK378 pKa = 10.33 AVSFFEE384 pKa = 4.08 TAINSII390 pKa = 4.07
Molecular weight: 45.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.135
IPC2_protein 9.472
IPC_protein 9.428
Toseland 10.131
ProMoST 9.78
Dawson 10.306
Bjellqvist 9.955
Wikipedia 10.467
Rodwell 10.833
Grimsley 10.379
Solomon 10.321
Lehninger 10.292
Nozaki 10.101
DTASelect 9.955
Thurlkill 10.16
EMBOSS 10.526
Sillero 10.218
Patrickios 10.423
IPC_peptide 10.321
IPC2_peptide 8.463
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25
0
25
3928
46
545
157.1
18.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.779 ± 0.365
0.636 ± 0.241
5.524 ± 0.467
8.248 ± 0.51
4.302 ± 0.175
4.786 ± 0.33
2.062 ± 0.282
7.281 ± 0.432
9.114 ± 0.51
9.878 ± 0.391
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.245
5.779 ± 0.392
3.106 ± 0.396
4.761 ± 0.358
5.219 ± 0.356
4.888 ± 0.277
5.881 ± 0.492
4.582 ± 0.35
0.942 ± 0.101
4.633 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here