Streptomyces phage Kardashian

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bingvirus; unclassified Bingvirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6D7U7|A0A5J6D7U7_9CAUD Uncharacterized protein OS=Streptomyces phage Kardashian OX=2601691 GN=32 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84DD4 pKa = 3.32YY5 pKa = 10.79MVTVKK10 pKa = 10.39EE11 pKa = 4.11NPYY14 pKa = 10.4NYY16 pKa = 8.91YY17 pKa = 10.1TEE19 pKa = 4.87FEE21 pKa = 4.03DD22 pKa = 3.6WYY24 pKa = 11.04RR25 pKa = 11.84WDD27 pKa = 4.25TINGYY32 pKa = 8.12HH33 pKa = 5.87TLSYY37 pKa = 9.62LAKK40 pKa = 9.11VTRR43 pKa = 11.84TSDD46 pKa = 3.26ALSPQLDD53 pKa = 3.5EE54 pKa = 5.62DD55 pKa = 5.07AISDD59 pKa = 3.86AVDD62 pKa = 4.22EE63 pKa = 4.47ILSEE67 pKa = 4.23NVGGIYY73 pKa = 10.17TKK75 pKa = 10.64VEE77 pKa = 3.84QPKK80 pKa = 10.56DD81 pKa = 3.77YY82 pKa = 11.12VPSAEE87 pKa = 3.91

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6D7Z4|A0A5J6D7Z4_9CAUD Uncharacterized protein OS=Streptomyces phage Kardashian OX=2601691 GN=74 PE=4 SV=1
MM1 pKa = 6.95VAIAVAIDD9 pKa = 3.38AGSAYY14 pKa = 10.49LAEE17 pKa = 4.21RR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 7.93TCTKK24 pKa = 10.36KK25 pKa = 10.76FEE27 pKa = 4.48AEE29 pKa = 4.12VKK31 pKa = 10.36RR32 pKa = 11.84GISAYY37 pKa = 10.2RR38 pKa = 11.84NRR40 pKa = 11.84KK41 pKa = 9.3KK42 pKa = 10.36IEE44 pKa = 4.11SGLAMISQRR53 pKa = 11.84KK54 pKa = 7.87EE55 pKa = 3.83SVVRR59 pKa = 11.84PRR61 pKa = 11.84GTHH64 pKa = 5.96RR65 pKa = 11.84RR66 pKa = 11.84LQVSS70 pKa = 3.73

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

17246

33

1829

207.8

23.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.057 ± 0.613

0.516 ± 0.098

6.297 ± 0.353

7.057 ± 0.392

3.589 ± 0.209

7.422 ± 0.413

1.751 ± 0.185

5.259 ± 0.218

6.715 ± 0.37

8.089 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.125

4.123 ± 0.131

4.013 ± 0.243

3.363 ± 0.153

5.416 ± 0.437

6.141 ± 0.267

6.604 ± 0.344

7.057 ± 0.158

1.374 ± 0.154

3.52 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski