Bifidobacterium phage BigBern1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XZT2|A0A6G6XZT2_9CAUD Uncharacterized protein OS=Bifidobacterium phage BigBern1 OX=2713246 GN=BIBE0010001c01_00018 PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 2.96MSFPAHH9 pKa = 6.15TLACDD14 pKa = 3.35WPSCDD19 pKa = 4.79QIAHH23 pKa = 6.64DD24 pKa = 4.75GEE26 pKa = 4.35YY27 pKa = 10.52SAWEE31 pKa = 4.4SYY33 pKa = 11.16DD34 pKa = 3.42SAVTMFADD42 pKa = 4.3PDD44 pKa = 3.75NDD46 pKa = 4.2GEE48 pKa = 4.25WLHH51 pKa = 6.34TEE53 pKa = 4.56DD54 pKa = 5.41GRR56 pKa = 11.84DD57 pKa = 3.48YY58 pKa = 11.12CPKK61 pKa = 9.85HH62 pKa = 4.58WHH64 pKa = 6.79IDD66 pKa = 3.34DD67 pKa = 5.32DD68 pKa = 4.64LDD70 pKa = 3.85EE71 pKa = 4.98RR72 pKa = 11.84VPGPDD77 pKa = 3.81PEE79 pKa = 4.45DD80 pKa = 3.4SKK82 pKa = 11.82NLEE85 pKa = 4.0EE86 pKa = 4.41QAA88 pKa = 4.05

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6Y0V5|A0A6G6Y0V5_9CAUD Uncharacterized protein OS=Bifidobacterium phage BigBern1 OX=2713246 GN=BIBE0010001c01_00020 PE=4 SV=1
MM1 pKa = 7.81LIVCPGALRR10 pKa = 11.84ADD12 pKa = 4.22FQRR15 pKa = 11.84WYY17 pKa = 11.09GLNLDD22 pKa = 4.75DD23 pKa = 5.81LGHH26 pKa = 6.11SLRR29 pKa = 11.84IRR31 pKa = 11.84RR32 pKa = 11.84AADD35 pKa = 3.3LAAYY39 pKa = 9.21IPEE42 pKa = 5.39DD43 pKa = 3.55GAVWPEE49 pKa = 3.23IDD51 pKa = 4.19PRR53 pKa = 11.84LRR55 pKa = 11.84WGTTKK60 pKa = 10.51QLLANISDD68 pKa = 3.84ATSFTAWTKK77 pKa = 9.81TKK79 pKa = 10.45DD80 pKa = 3.73AQRR83 pKa = 11.84HH84 pKa = 3.79NATWKK89 pKa = 10.56GALQRR94 pKa = 11.84PGMHH98 pKa = 5.82EE99 pKa = 3.73HH100 pKa = 6.77RR101 pKa = 11.84TRR103 pKa = 11.84QHH105 pKa = 5.83GVQAMSPEE113 pKa = 4.0QLDD116 pKa = 3.39RR117 pKa = 11.84MLSMRR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84AAPPQNN130 pKa = 3.37

Molecular weight: 14.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11947

40

978

192.7

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.329 ± 0.433

1.197 ± 0.181

6.897 ± 0.433

5.206 ± 0.434

2.235 ± 0.18

8.504 ± 0.425

2.017 ± 0.18

4.955 ± 0.293

4.336 ± 0.314

7.985 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.804 ± 0.166

2.829 ± 0.23

5.357 ± 0.235

4.947 ± 0.268

6.772 ± 0.497

6.437 ± 0.405

5.976 ± 0.262

6.211 ± 0.337

2.051 ± 0.159

2.955 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski