Staphylococcus virus SA11
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 186 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7D9X4|I7D9X4_9CAUD Uncharacterized protein OS=Staphylococcus virus SA11 OX=1204543 PE=4 SV=1
MM1 pKa = 7.7 LEE3 pKa = 3.83 QAKK6 pKa = 10.69 GITTDD11 pKa = 3.86 YY12 pKa = 10.97 IMEE15 pKa = 5.36 FIDD18 pKa = 3.39 EE19 pKa = 4.46 HH20 pKa = 4.75 EE21 pKa = 4.41 HH22 pKa = 6.22 YY23 pKa = 10.25 FYY25 pKa = 11.48 EE26 pKa = 5.01 DD27 pKa = 3.21 LHH29 pKa = 8.19 KK30 pKa = 10.89 LAGEE34 pKa = 4.14 LVLKK38 pKa = 8.97 DD39 pKa = 3.47 THH41 pKa = 6.45 DD42 pKa = 3.75 TNPIVINDD50 pKa = 3.41 GDD52 pKa = 4.5 GYY54 pKa = 10.99 SGTIISKK61 pKa = 10.05 FKK63 pKa = 11.0 SKK65 pKa = 11.26 VEE67 pKa = 3.99 GVPDD71 pKa = 3.21 ITVSDD76 pKa = 4.67 FYY78 pKa = 11.38 GSCSFCDD85 pKa = 3.32 TLTNVYY91 pKa = 9.96 EE92 pKa = 4.95 DD93 pKa = 3.69 SDD95 pKa = 3.81 EE96 pKa = 4.64 LEE98 pKa = 4.69 IIRR101 pKa = 11.84 DD102 pKa = 3.54 LATMVLHH109 pKa = 7.14 TIQSMEE115 pKa = 4.15 EE116 pKa = 3.75 QVEE119 pKa = 4.09
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.859
IPC2_protein 4.113
IPC_protein 4.062
Toseland 3.884
ProMoST 4.164
Dawson 4.037
Bjellqvist 4.228
Wikipedia 3.935
Rodwell 3.897
Grimsley 3.795
Solomon 4.024
Lehninger 3.986
Nozaki 4.151
DTASelect 4.329
Thurlkill 3.91
EMBOSS 3.948
Sillero 4.177
Patrickios 1.939
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.084
Protein with the highest isoelectric point:
>tr|I7D9W7|I7D9W7_9CAUD Uncharacterized protein OS=Staphylococcus virus SA11 OX=1204543 PE=4 SV=1
MM1 pKa = 7.74 RR2 pKa = 11.84 DD3 pKa = 2.71 IRR5 pKa = 11.84 IVLRR9 pKa = 11.84 EE10 pKa = 3.75 IDD12 pKa = 4.01 EE13 pKa = 4.3 VQQALIYY20 pKa = 10.08 GRR22 pKa = 11.84 NSRR25 pKa = 11.84 EE26 pKa = 3.41 LARR29 pKa = 11.84 FLRR32 pKa = 11.84 RR33 pKa = 11.84 QLVKK37 pKa = 10.69 LEE39 pKa = 4.7 KK40 pKa = 10.12 EE41 pKa = 3.95 RR42 pKa = 11.84 QDD44 pKa = 3.69 INWSS48 pKa = 3.36
Molecular weight: 5.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.27
IPC2_protein 9.297
IPC_protein 10.058
Toseland 10.394
ProMoST 10.087
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.716
Rodwell 10.599
Grimsley 10.555
Solomon 10.657
Lehninger 10.628
Nozaki 10.35
DTASelect 10.218
Thurlkill 10.394
EMBOSS 10.804
Sillero 10.438
Patrickios 10.496
IPC_peptide 10.657
IPC2_peptide 8.916
IPC2.peptide.svr19 8.668
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
186
0
186
39194
38
1268
210.7
24.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.998 ± 0.183
0.681 ± 0.055
6.927 ± 0.14
7.56 ± 0.286
3.735 ± 0.103
5.419 ± 0.259
1.62 ± 0.09
7.468 ± 0.179
9.69 ± 0.226
8.42 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.261 ± 0.096
7.05 ± 0.219
2.569 ± 0.138
3.462 ± 0.144
3.488 ± 0.128
6.595 ± 0.205
6.445 ± 0.216
6.389 ± 0.179
0.842 ± 0.063
5.381 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here