Amino acid dipepetide frequency for Escherichia phage 121Q

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.218AlaAla: 3.218 ± 0.276
0.522AlaCys: 0.522 ± 0.066
3.041AlaAsp: 3.041 ± 0.187
3.498AlaGlu: 3.498 ± 0.197
1.912AlaPhe: 1.912 ± 0.136
3.05AlaGly: 3.05 ± 0.222
0.625AlaHis: 0.625 ± 0.076
3.376AlaIle: 3.376 ± 0.174
3.581AlaLys: 3.581 ± 0.202
4.197AlaLeu: 4.197 ± 0.204
1.324AlaMet: 1.324 ± 0.114
2.751AlaAsn: 2.751 ± 0.172
1.707AlaPro: 1.707 ± 0.158
1.865AlaGln: 1.865 ± 0.133
1.912AlaArg: 1.912 ± 0.151
3.069AlaSer: 3.069 ± 0.242
2.751AlaThr: 2.751 ± 0.313
3.404AlaVal: 3.404 ± 0.203
0.588AlaTrp: 0.588 ± 0.067
2.192AlaTyr: 2.192 ± 0.129
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.075
0.205CysCys: 0.205 ± 0.057
0.895CysAsp: 0.895 ± 0.093
0.746CysGlu: 0.746 ± 0.081
0.597CysPhe: 0.597 ± 0.079
0.933CysGly: 0.933 ± 0.113
0.233CysHis: 0.233 ± 0.046
1.334CysIle: 1.334 ± 0.133
0.923CysLys: 0.923 ± 0.085
0.858CysLeu: 0.858 ± 0.093
0.242CysMet: 0.242 ± 0.053
0.793CysAsn: 0.793 ± 0.094
0.429CysPro: 0.429 ± 0.068
0.401CysGln: 0.401 ± 0.062
0.42CysArg: 0.42 ± 0.077
1.017CysSer: 1.017 ± 0.113
1.175CysThr: 1.175 ± 0.127
1.007CysVal: 1.007 ± 0.097
0.14CysTrp: 0.14 ± 0.038
0.56CysTyr: 0.56 ± 0.059
0.0CysXaa: 0.0 ± 0.0
Asp
3.656AspAla: 3.656 ± 0.214
0.783AspCys: 0.783 ± 0.113
4.188AspAsp: 4.188 ± 0.195
4.691AspGlu: 4.691 ± 0.243
3.152AspPhe: 3.152 ± 0.169
4.645AspGly: 4.645 ± 0.239
0.755AspHis: 0.755 ± 0.077
5.848AspIle: 5.848 ± 0.255
4.085AspLys: 4.085 ± 0.208
5.204AspLeu: 5.204 ± 0.257
2.061AspMet: 2.061 ± 0.147
3.843AspAsn: 3.843 ± 0.21
2.052AspPro: 2.052 ± 0.142
1.175AspGln: 1.175 ± 0.113
1.949AspArg: 1.949 ± 0.151
4.505AspSer: 4.505 ± 0.235
3.46AspThr: 3.46 ± 0.193
4.551AspVal: 4.551 ± 0.231
0.727AspTrp: 0.727 ± 0.088
3.656AspTyr: 3.656 ± 0.208
0.0AspXaa: 0.0 ± 0.0
Glu
2.966GluAla: 2.966 ± 0.196
0.895GluCys: 0.895 ± 0.104
4.169GluAsp: 4.169 ± 0.238
5.092GluGlu: 5.092 ± 0.322
3.404GluPhe: 3.404 ± 0.18
2.826GluGly: 2.826 ± 0.195
1.614GluHis: 1.614 ± 0.123
5.307GluIle: 5.307 ± 0.225
4.085GluLys: 4.085 ± 0.23
6.333GluLeu: 6.333 ± 0.341
2.667GluMet: 2.667 ± 0.192
3.619GluAsn: 3.619 ± 0.213
1.921GluPro: 1.921 ± 0.142
2.714GluGln: 2.714 ± 0.174
2.49GluArg: 2.49 ± 0.146
4.15GluSer: 4.15 ± 0.185
3.246GluThr: 3.246 ± 0.174
4.505GluVal: 4.505 ± 0.19
0.923GluTrp: 0.923 ± 0.091
4.216GluTyr: 4.216 ± 0.238
0.0GluXaa: 0.0 ± 0.0
Phe
1.753PheAla: 1.753 ± 0.132
0.755PheCys: 0.755 ± 0.087
3.274PheAsp: 3.274 ± 0.191
2.742PheGlu: 2.742 ± 0.141
1.903PhePhe: 1.903 ± 0.161
2.91PheGly: 2.91 ± 0.175
0.811PheHis: 0.811 ± 0.099
3.955PheIle: 3.955 ± 0.219
3.563PheLys: 3.563 ± 0.191
2.929PheLeu: 2.929 ± 0.17
1.455PheMet: 1.455 ± 0.12
3.255PheAsn: 3.255 ± 0.158
1.175PhePro: 1.175 ± 0.105
1.819PheGln: 1.819 ± 0.129
1.52PheArg: 1.52 ± 0.13
3.386PheSer: 3.386 ± 0.191
2.621PheThr: 2.621 ± 0.175
3.022PheVal: 3.022 ± 0.18
0.55PheTrp: 0.55 ± 0.068
2.229PheTyr: 2.229 ± 0.126
0.0PheXaa: 0.0 ± 0.0
Gly
2.957GlyAla: 2.957 ± 0.288
0.933GlyCys: 0.933 ± 0.113
3.208GlyAsp: 3.208 ± 0.223
3.554GlyGlu: 3.554 ± 0.203
2.677GlyPhe: 2.677 ± 0.158
3.376GlyGly: 3.376 ± 0.374
0.793GlyHis: 0.793 ± 0.082
3.889GlyIle: 3.889 ± 0.203
4.113GlyLys: 4.113 ± 0.236
3.936GlyLeu: 3.936 ± 0.191
1.408GlyMet: 1.408 ± 0.13
3.955GlyAsn: 3.955 ± 0.245
0.616GlyPro: 0.616 ± 0.073
1.763GlyGln: 1.763 ± 0.149
2.08GlyArg: 2.08 ± 0.128
4.43GlySer: 4.43 ± 0.291
5.13GlyThr: 5.13 ± 0.369
4.188GlyVal: 4.188 ± 0.21
0.616GlyTrp: 0.616 ± 0.088
2.919GlyTyr: 2.919 ± 0.173
0.0GlyXaa: 0.0 ± 0.0
His
0.811HisAla: 0.811 ± 0.089
0.289HisCys: 0.289 ± 0.06
1.427HisAsp: 1.427 ± 0.13
1.446HisGlu: 1.446 ± 0.124
0.914HisPhe: 0.914 ± 0.093
0.923HisGly: 0.923 ± 0.09
0.485HisHis: 0.485 ± 0.076
1.483HisIle: 1.483 ± 0.127
1.586HisLys: 1.586 ± 0.113
1.362HisLeu: 1.362 ± 0.108
0.597HisMet: 0.597 ± 0.079
1.259HisAsn: 1.259 ± 0.113
0.69HisPro: 0.69 ± 0.08
0.504HisGln: 0.504 ± 0.06
0.746HisArg: 0.746 ± 0.08
1.157HisSer: 1.157 ± 0.11
1.073HisThr: 1.073 ± 0.088
0.942HisVal: 0.942 ± 0.106
0.242HisTrp: 0.242 ± 0.048
0.933HisTyr: 0.933 ± 0.099
0.0HisXaa: 0.0 ± 0.0
Ile
3.498IleAla: 3.498 ± 0.173
0.905IleCys: 0.905 ± 0.099
5.699IleAsp: 5.699 ± 0.286
5.559IleGlu: 5.559 ± 0.239
2.537IlePhe: 2.537 ± 0.164
3.852IleGly: 3.852 ± 0.215
1.772IleHis: 1.772 ± 0.126
5.326IleIle: 5.326 ± 0.238
5.54IleLys: 5.54 ± 0.243
5.186IleLeu: 5.186 ± 0.214
2.052IleMet: 2.052 ± 0.146
5.018IleAsn: 5.018 ± 0.217
2.798IlePro: 2.798 ± 0.179
3.078IleGln: 3.078 ± 0.177
3.32IleArg: 3.32 ± 0.172
5.605IleSer: 5.605 ± 0.259
4.925IleThr: 4.925 ± 0.311
4.384IleVal: 4.384 ± 0.236
0.765IleTrp: 0.765 ± 0.096
3.041IleTyr: 3.041 ± 0.181
0.0IleXaa: 0.0 ± 0.0
Lys
3.33LysAla: 3.33 ± 0.189
0.905LysCys: 0.905 ± 0.099
4.402LysAsp: 4.402 ± 0.227
5.438LysGlu: 5.438 ± 0.282
3.339LysPhe: 3.339 ± 0.188
2.817LysGly: 2.817 ± 0.16
1.418LysHis: 1.418 ± 0.123
5.167LysIle: 5.167 ± 0.248
5.176LysLys: 5.176 ± 0.271
5.466LysLeu: 5.466 ± 0.223
2.537LysMet: 2.537 ± 0.162
4.318LysAsn: 4.318 ± 0.221
1.903LysPro: 1.903 ± 0.152
2.332LysGln: 2.332 ± 0.181
2.994LysArg: 2.994 ± 0.195
4.579LysSer: 4.579 ± 0.216
3.955LysThr: 3.955 ± 0.186
4.626LysVal: 4.626 ± 0.204
0.597LysTrp: 0.597 ± 0.072
4.542LysTyr: 4.542 ± 0.205
0.0LysXaa: 0.0 ± 0.0
Leu
3.712LeuAla: 3.712 ± 0.228
1.175LeuCys: 1.175 ± 0.106
5.447LeuAsp: 5.447 ± 0.273
4.971LeuGlu: 4.971 ± 0.24
3.115LeuPhe: 3.115 ± 0.167
4.188LeuGly: 4.188 ± 0.205
1.586LeuHis: 1.586 ± 0.11
5.055LeuIle: 5.055 ± 0.241
5.839LeuLys: 5.839 ± 0.283
5.839LeuLeu: 5.839 ± 0.274
2.658LeuMet: 2.658 ± 0.177
5.316LeuAsn: 5.316 ± 0.277
2.929LeuPro: 2.929 ± 0.173
3.003LeuGln: 3.003 ± 0.16
3.087LeuArg: 3.087 ± 0.183
6.053LeuSer: 6.053 ± 0.237
4.766LeuThr: 4.766 ± 0.303
5.251LeuVal: 5.251 ± 0.235
0.849LeuTrp: 0.849 ± 0.092
4.3LeuTyr: 4.3 ± 0.231
0.0LeuXaa: 0.0 ± 0.0
Met
1.492MetAla: 1.492 ± 0.123
0.326MetCys: 0.326 ± 0.055
1.595MetAsp: 1.595 ± 0.137
1.492MetGlu: 1.492 ± 0.116
1.502MetPhe: 1.502 ± 0.122
1.231MetGly: 1.231 ± 0.1
0.662MetHis: 0.662 ± 0.08
2.108MetIle: 2.108 ± 0.146
2.761MetLys: 2.761 ± 0.183
2.238MetLeu: 2.238 ± 0.157
0.811MetMet: 0.811 ± 0.104
2.099MetAsn: 2.099 ± 0.145
0.644MetPro: 0.644 ± 0.072
1.147MetGln: 1.147 ± 0.093
1.11MetArg: 1.11 ± 0.11
1.996MetSer: 1.996 ± 0.144
1.772MetThr: 1.772 ± 0.129
1.576MetVal: 1.576 ± 0.127
0.298MetTrp: 0.298 ± 0.052
1.884MetTyr: 1.884 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.348AsnAla: 3.348 ± 0.22
0.746AsnCys: 0.746 ± 0.1
3.964AsnAsp: 3.964 ± 0.221
4.206AsnGlu: 4.206 ± 0.202
2.761AsnPhe: 2.761 ± 0.14
4.775AsnGly: 4.775 ± 0.299
1.212AsnHis: 1.212 ± 0.114
5.605AsnIle: 5.605 ± 0.258
3.787AsnLys: 3.787 ± 0.186
4.384AsnLeu: 4.384 ± 0.191
1.399AsnMet: 1.399 ± 0.118
4.393AsnAsn: 4.393 ± 0.204
2.556AsnPro: 2.556 ± 0.189
1.847AsnGln: 1.847 ± 0.134
2.229AsnArg: 2.229 ± 0.162
4.346AsnSer: 4.346 ± 0.211
4.766AsnThr: 4.766 ± 0.252
4.141AsnVal: 4.141 ± 0.215
0.466AsnTrp: 0.466 ± 0.073
2.686AsnTyr: 2.686 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
1.567ProAla: 1.567 ± 0.146
0.28ProCys: 0.28 ± 0.053
2.472ProAsp: 2.472 ± 0.144
2.733ProGlu: 2.733 ± 0.166
1.408ProPhe: 1.408 ± 0.078
1.52ProGly: 1.52 ± 0.122
0.476ProHis: 0.476 ± 0.069
1.977ProIle: 1.977 ± 0.141
2.005ProLys: 2.005 ± 0.159
2.117ProLeu: 2.117 ± 0.123
0.606ProMet: 0.606 ± 0.07
2.099ProAsn: 2.099 ± 0.145
0.774ProPro: 0.774 ± 0.086
0.951ProGln: 0.951 ± 0.09
0.961ProArg: 0.961 ± 0.105
2.397ProSer: 2.397 ± 0.138
1.828ProThr: 1.828 ± 0.142
2.453ProVal: 2.453 ± 0.161
0.298ProTrp: 0.298 ± 0.054
1.688ProTyr: 1.688 ± 0.13
0.0ProXaa: 0.0 ± 0.0
Gln
1.819GlnAla: 1.819 ± 0.117
0.569GlnCys: 0.569 ± 0.076
1.949GlnAsp: 1.949 ± 0.122
2.574GlnGlu: 2.574 ± 0.162
1.697GlnPhe: 1.697 ± 0.148
1.893GlnGly: 1.893 ± 0.126
0.802GlnHis: 0.802 ± 0.083
2.033GlnIle: 2.033 ± 0.146
2.266GlnLys: 2.266 ± 0.152
3.124GlnLeu: 3.124 ± 0.186
1.11GlnMet: 1.11 ± 0.11
2.015GlnAsn: 2.015 ± 0.148
1.007GlnPro: 1.007 ± 0.112
1.614GlnGln: 1.614 ± 0.131
1.464GlnArg: 1.464 ± 0.114
2.49GlnSer: 2.49 ± 0.155
1.688GlnThr: 1.688 ± 0.128
1.996GlnVal: 1.996 ± 0.136
0.364GlnTrp: 0.364 ± 0.061
2.779GlnTyr: 2.779 ± 0.166
0.0GlnXaa: 0.0 ± 0.0
Arg
1.707ArgAla: 1.707 ± 0.142
0.588ArgCys: 0.588 ± 0.08
2.182ArgAsp: 2.182 ± 0.146
2.35ArgGlu: 2.35 ± 0.167
1.763ArgPhe: 1.763 ± 0.132
2.061ArgGly: 2.061 ± 0.132
0.625ArgHis: 0.625 ± 0.087
2.938ArgIle: 2.938 ± 0.167
2.574ArgLys: 2.574 ± 0.182
2.77ArgLeu: 2.77 ± 0.164
1.268ArgMet: 1.268 ± 0.094
2.49ArgAsn: 2.49 ± 0.149
0.979ArgPro: 0.979 ± 0.098
1.595ArgGln: 1.595 ± 0.124
1.296ArgArg: 1.296 ± 0.117
2.546ArgSer: 2.546 ± 0.157
2.257ArgThr: 2.257 ± 0.149
2.192ArgVal: 2.192 ± 0.168
0.513ArgTrp: 0.513 ± 0.077
2.099ArgTyr: 2.099 ± 0.144
0.0ArgXaa: 0.0 ± 0.0
Ser
3.46SerAla: 3.46 ± 0.207
0.951SerCys: 0.951 ± 0.109
3.871SerAsp: 3.871 ± 0.21
4.02SerGlu: 4.02 ± 0.192
3.526SerPhe: 3.526 ± 0.193
4.551SerGly: 4.551 ± 0.349
1.054SerHis: 1.054 ± 0.106
5.493SerIle: 5.493 ± 0.257
4.691SerLys: 4.691 ± 0.237
5.876SerLeu: 5.876 ± 0.23
1.614SerMet: 1.614 ± 0.142
4.225SerAsn: 4.225 ± 0.211
1.987SerPro: 1.987 ± 0.107
1.912SerGln: 1.912 ± 0.154
2.192SerArg: 2.192 ± 0.139
5.419SerSer: 5.419 ± 0.306
6.697SerThr: 6.697 ± 0.326
5.064SerVal: 5.064 ± 0.251
0.672SerTrp: 0.672 ± 0.082
3.442SerTyr: 3.442 ± 0.185
0.0SerXaa: 0.0 ± 0.0
Thr
3.33ThrAla: 3.33 ± 0.332
0.578ThrCys: 0.578 ± 0.079
4.197ThrAsp: 4.197 ± 0.206
4.104ThrGlu: 4.104 ± 0.189
3.134ThrPhe: 3.134 ± 0.187
4.272ThrGly: 4.272 ± 0.419
1.054ThrHis: 1.054 ± 0.093
4.953ThrIle: 4.953 ± 0.274
4.029ThrLys: 4.029 ± 0.202
5.671ThrLeu: 5.671 ± 0.319
1.474ThrMet: 1.474 ± 0.114
3.973ThrAsn: 3.973 ± 0.238
2.322ThrPro: 2.322 ± 0.172
2.164ThrGln: 2.164 ± 0.148
2.015ThrArg: 2.015 ± 0.139
4.356ThrSer: 4.356 ± 0.283
4.094ThrThr: 4.094 ± 0.385
4.841ThrVal: 4.841 ± 0.361
0.774ThrTrp: 0.774 ± 0.085
3.171ThrTyr: 3.171 ± 0.177
0.0ThrXaa: 0.0 ± 0.0
Val
2.612ValAla: 2.612 ± 0.202
0.905ValCys: 0.905 ± 0.099
4.132ValAsp: 4.132 ± 0.224
4.104ValGlu: 4.104 ± 0.206
3.087ValPhe: 3.087 ± 0.194
3.069ValGly: 3.069 ± 0.216
1.614ValHis: 1.614 ± 0.123
4.514ValIle: 4.514 ± 0.199
4.803ValLys: 4.803 ± 0.216
7.275ValLeu: 7.275 ± 0.322
1.642ValMet: 1.642 ± 0.131
3.423ValAsn: 3.423 ± 0.176
2.686ValPro: 2.686 ± 0.208
3.134ValGln: 3.134 ± 0.172
2.332ValArg: 2.332 ± 0.159
4.981ValSer: 4.981 ± 0.288
3.833ValThr: 3.833 ± 0.367
4.011ValVal: 4.011 ± 0.228
0.727ValTrp: 0.727 ± 0.094
3.442ValTyr: 3.442 ± 0.196
0.0ValXaa: 0.0 ± 0.0
Trp
0.513TrpAla: 0.513 ± 0.074
0.252TrpCys: 0.252 ± 0.05
0.839TrpAsp: 0.839 ± 0.096
0.653TrpGlu: 0.653 ± 0.08
0.588TrpPhe: 0.588 ± 0.079
0.569TrpGly: 0.569 ± 0.077
0.215TrpHis: 0.215 ± 0.043
0.755TrpIle: 0.755 ± 0.08
0.681TrpLys: 0.681 ± 0.082
0.681TrpLeu: 0.681 ± 0.076
0.345TrpMet: 0.345 ± 0.045
0.727TrpAsn: 0.727 ± 0.088
0.149TrpPro: 0.149 ± 0.04
0.242TrpGln: 0.242 ± 0.053
0.41TrpArg: 0.41 ± 0.063
0.606TrpSer: 0.606 ± 0.055
0.755TrpThr: 0.755 ± 0.091
0.821TrpVal: 0.821 ± 0.088
0.149TrpTrp: 0.149 ± 0.038
0.821TrpTyr: 0.821 ± 0.094
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.248TyrAla: 2.248 ± 0.163
0.933TyrCys: 0.933 ± 0.103
4.039TyrAsp: 4.039 ± 0.228
3.171TyrGlu: 3.171 ± 0.174
2.612TyrPhe: 2.612 ± 0.158
3.078TyrGly: 3.078 ± 0.199
1.063TyrHis: 1.063 ± 0.113
3.74TyrIle: 3.74 ± 0.21
3.749TyrLys: 3.749 ± 0.213
3.693TyrLeu: 3.693 ± 0.201
1.464TyrMet: 1.464 ± 0.133
4.048TyrAsn: 4.048 ± 0.209
1.287TyrPro: 1.287 ± 0.098
1.996TyrGln: 1.996 ± 0.168
2.266TyrArg: 2.266 ± 0.152
3.628TyrSer: 3.628 ± 0.178
3.712TyrThr: 3.712 ± 0.195
3.358TyrVal: 3.358 ± 0.189
0.513TyrTrp: 0.513 ± 0.08
2.985TyrTyr: 2.985 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 611 proteins (107219 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski