Agromyces sp. NDB4Y10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces; unclassified Agromyces

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165S8V8|A0A165S8V8_9MICO Nicotinate dehydrogenase FAD-subunit OS=Agromyces sp. NDB4Y10 OX=1775951 GN=ndhF PE=4 SV=1
MM1 pKa = 7.62GNPVLRR7 pKa = 11.84APRR10 pKa = 11.84RR11 pKa = 11.84SIGAFAVGALAASFLAATAAPANADD36 pKa = 3.38DD37 pKa = 4.81HH38 pKa = 7.12EE39 pKa = 4.63NLPGEE44 pKa = 4.2PTITLPINDD53 pKa = 3.78SAAGGTYY60 pKa = 10.54DD61 pKa = 4.24QDD63 pKa = 3.27FTRR66 pKa = 11.84AYY68 pKa = 10.6AGATPDD74 pKa = 3.42GTPVYY79 pKa = 9.93LYY81 pKa = 10.87VPAGTPLDD89 pKa = 3.73GSAPVGVDD97 pKa = 3.09SLDD100 pKa = 3.27PAFDD104 pKa = 4.52HH105 pKa = 7.13NPDD108 pKa = 4.05DD109 pKa = 5.85DD110 pKa = 4.87FVPCADD116 pKa = 4.35RR117 pKa = 11.84DD118 pKa = 4.06VADD121 pKa = 4.13FTLTQAQIDD130 pKa = 4.11YY131 pKa = 10.29MGDD134 pKa = 2.99QLANQIVAVDD144 pKa = 4.02EE145 pKa = 4.38EE146 pKa = 5.08HH147 pKa = 6.85FGPMDD152 pKa = 3.99AADD155 pKa = 4.17PDD157 pKa = 4.19VPEE160 pKa = 4.57SDD162 pKa = 3.72SLVMLVYY169 pKa = 10.51NVQDD173 pKa = 3.56DD174 pKa = 4.19NYY176 pKa = 9.32YY177 pKa = 10.9DD178 pKa = 3.98CAATSYY184 pKa = 9.15TAGYY188 pKa = 7.64FAPDD192 pKa = 5.0FIDD195 pKa = 3.48SAGMNTIVIDD205 pKa = 3.91AFDD208 pKa = 3.41WANRR212 pKa = 11.84VGPNDD217 pKa = 3.68AEE219 pKa = 4.23WRR221 pKa = 11.84DD222 pKa = 3.5GDD224 pKa = 4.35PANDD228 pKa = 4.07LPTLYY233 pKa = 10.07EE234 pKa = 3.82GVIAHH239 pKa = 6.11EE240 pKa = 4.63LEE242 pKa = 4.78HH243 pKa = 6.83LLHH246 pKa = 6.67NYY248 pKa = 9.83SDD250 pKa = 4.0PGEE253 pKa = 4.2LSWVDD258 pKa = 3.2EE259 pKa = 4.26GLADD263 pKa = 4.63FAVFLNGYY271 pKa = 9.39DD272 pKa = 3.18VGGSHH277 pKa = 6.53LTYY280 pKa = 10.21HH281 pKa = 5.09QVFYY285 pKa = 11.44AEE287 pKa = 4.24TSLTRR292 pKa = 11.84WAGGLEE298 pKa = 4.28NYY300 pKa = 8.69GAAYY304 pKa = 9.07TYY306 pKa = 8.87MQYY309 pKa = 11.01LWEE312 pKa = 4.09QAGGNGDD319 pKa = 3.68GTYY322 pKa = 10.66EE323 pKa = 4.55PDD325 pKa = 4.34LEE327 pKa = 4.13YY328 pKa = 11.16DD329 pKa = 3.67GAGGDD334 pKa = 4.39LLIKK338 pKa = 10.68LIFEE342 pKa = 4.25NQADD346 pKa = 3.78GMAGVQAAIDD356 pKa = 3.98EE357 pKa = 4.78FNAATGADD365 pKa = 3.48LRR367 pKa = 11.84SAEE370 pKa = 4.82EE371 pKa = 4.81LFQDD375 pKa = 3.53WAVAVYY381 pKa = 10.77LDD383 pKa = 5.07DD384 pKa = 4.6EE385 pKa = 4.84DD386 pKa = 5.61SEE388 pKa = 5.15RR389 pKa = 11.84FDD391 pKa = 3.49IKK393 pKa = 10.99AVDD396 pKa = 4.16FGDD399 pKa = 3.94PASTSWTIDD408 pKa = 3.03IADD411 pKa = 3.66DD412 pKa = 3.82QFWDD416 pKa = 4.14GRR418 pKa = 11.84GSNQGAQPDD427 pKa = 4.21AKK429 pKa = 10.01WEE431 pKa = 3.98RR432 pKa = 11.84RR433 pKa = 11.84KK434 pKa = 10.61NGQADD439 pKa = 3.38TALPYY444 pKa = 10.45GIQVEE449 pKa = 4.58RR450 pKa = 11.84FRR452 pKa = 11.84NPGPTVTVGLDD463 pKa = 3.46GDD465 pKa = 4.45DD466 pKa = 3.63TTQIAPHH473 pKa = 6.52TGDD476 pKa = 3.43THH478 pKa = 6.68WYY480 pKa = 9.87AGYY483 pKa = 10.35EE484 pKa = 4.15SQADD488 pKa = 3.45NVLDD492 pKa = 3.83VEE494 pKa = 4.71VADD497 pKa = 4.99GAASTLDD504 pKa = 3.23FWSWHH509 pKa = 6.06FIEE512 pKa = 5.46EE513 pKa = 4.19GWDD516 pKa = 3.44YY517 pKa = 11.6GFVEE521 pKa = 4.78ALVGDD526 pKa = 4.02EE527 pKa = 4.15WVTVPLTDD535 pKa = 4.77DD536 pKa = 3.36SGATVTTDD544 pKa = 3.24TDD546 pKa = 3.44PHH548 pKa = 6.44GNNAEE553 pKa = 4.26GNGLTGTSGGEE564 pKa = 3.98YY565 pKa = 10.19FVDD568 pKa = 3.76EE569 pKa = 4.66PEE571 pKa = 4.25YY572 pKa = 10.87VHH574 pKa = 7.26LSADD578 pKa = 3.95LPAGTTDD585 pKa = 2.73VRR587 pKa = 11.84FRR589 pKa = 11.84YY590 pKa = 8.58STDD593 pKa = 2.83AAYY596 pKa = 10.92LDD598 pKa = 4.21TGWFVDD604 pKa = 3.91DD605 pKa = 4.21VMVDD609 pKa = 3.5GEE611 pKa = 4.24AAAVSSADD619 pKa = 3.76GEE621 pKa = 4.55WIEE624 pKa = 4.49TTGLQDD630 pKa = 4.55NNWTLQVVANCDD642 pKa = 3.41LTPGVEE648 pKa = 4.21SDD650 pKa = 3.98FEE652 pKa = 4.36IVDD655 pKa = 3.38EE656 pKa = 5.01AGNHH660 pKa = 4.92VYY662 pKa = 10.43RR663 pKa = 11.84LEE665 pKa = 5.53GDD667 pKa = 4.37DD668 pKa = 4.1ISMSGLDD675 pKa = 3.51TSCAKK680 pKa = 10.46GKK682 pKa = 9.92NRR684 pKa = 11.84DD685 pKa = 3.3FATLVSNLPTGDD697 pKa = 5.35LDD699 pKa = 4.09TLDD702 pKa = 4.0AGYY705 pKa = 10.99SLGVTNTGKK714 pKa = 10.75GKK716 pKa = 10.56RR717 pKa = 11.84GG718 pKa = 3.26

Molecular weight: 77.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A164DAU2|A0A164DAU2_9MICO 1 2-phenylacetyl-CoA epoxidase subunit E OS=Agromyces sp. NDB4Y10 OX=1775951 GN=paaE PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3157

0

3157

1011233

32

2103

320.3

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.179 ± 0.066

0.455 ± 0.01

6.454 ± 0.038

6.114 ± 0.038

3.042 ± 0.026

9.167 ± 0.041

2.023 ± 0.02

4.14 ± 0.031

1.653 ± 0.032

10.014 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.014

1.744 ± 0.025

5.595 ± 0.033

2.479 ± 0.026

7.929 ± 0.059

5.056 ± 0.028

5.634 ± 0.035

9.199 ± 0.041

1.508 ± 0.019

1.904 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski