Amino acid dipepetide frequency for Pseudomonas sp. VI4.1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.06AlaAla: 12.06 ± 0.1
1.209AlaCys: 1.209 ± 0.024
5.719AlaAsp: 5.719 ± 0.056
6.402AlaGlu: 6.402 ± 0.06
3.925AlaPhe: 3.925 ± 0.058
8.6AlaGly: 8.6 ± 0.08
2.164AlaHis: 2.164 ± 0.031
5.47AlaIle: 5.47 ± 0.076
3.878AlaLys: 3.878 ± 0.058
13.051AlaLeu: 13.051 ± 0.101
2.986AlaMet: 2.986 ± 0.04
3.026AlaAsn: 3.026 ± 0.043
4.479AlaPro: 4.479 ± 0.06
5.0AlaGln: 5.0 ± 0.051
6.501AlaArg: 6.501 ± 0.067
6.139AlaSer: 6.139 ± 0.058
5.083AlaThr: 5.083 ± 0.054
7.473AlaVal: 7.473 ± 0.065
1.568AlaTrp: 1.568 ± 0.033
2.58AlaTyr: 2.58 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.125CysAla: 1.125 ± 0.027
0.16CysCys: 0.16 ± 0.009
0.578CysAsp: 0.578 ± 0.019
0.58CysGlu: 0.58 ± 0.02
0.378CysPhe: 0.378 ± 0.014
1.014CysGly: 1.014 ± 0.023
0.289CysHis: 0.289 ± 0.013
0.476CysIle: 0.476 ± 0.015
0.31CysLys: 0.31 ± 0.013
1.141CysLeu: 1.141 ± 0.022
0.229CysMet: 0.229 ± 0.011
0.29CysAsn: 0.29 ± 0.011
0.493CysPro: 0.493 ± 0.019
0.379CysGln: 0.379 ± 0.014
0.603CysArg: 0.603 ± 0.019
0.644CysSer: 0.644 ± 0.02
0.513CysThr: 0.513 ± 0.016
0.756CysVal: 0.756 ± 0.02
0.159CysTrp: 0.159 ± 0.01
0.267CysTyr: 0.267 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
5.457AspAla: 5.457 ± 0.053
0.558AspCys: 0.558 ± 0.016
2.909AspAsp: 2.909 ± 0.042
3.411AspGlu: 3.411 ± 0.046
2.142AspPhe: 2.142 ± 0.036
4.324AspGly: 4.324 ± 0.051
1.226AspHis: 1.226 ± 0.028
2.81AspIle: 2.81 ± 0.039
2.08AspLys: 2.08 ± 0.04
5.853AspLeu: 5.853 ± 0.064
1.268AspMet: 1.268 ± 0.026
1.644AspAsn: 1.644 ± 0.034
2.917AspPro: 2.917 ± 0.042
2.189AspGln: 2.189 ± 0.039
3.139AspArg: 3.139 ± 0.045
3.004AspSer: 3.004 ± 0.039
2.529AspThr: 2.529 ± 0.04
3.638AspVal: 3.638 ± 0.048
0.971AspTrp: 0.971 ± 0.025
1.768AspTyr: 1.768 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
5.995GluAla: 5.995 ± 0.057
0.492GluCys: 0.492 ± 0.015
2.527GluAsp: 2.527 ± 0.036
3.024GluGlu: 3.024 ± 0.049
1.94GluPhe: 1.94 ± 0.033
3.902GluGly: 3.902 ± 0.042
1.658GluHis: 1.658 ± 0.032
2.804GluIle: 2.804 ± 0.039
2.253GluLys: 2.253 ± 0.045
6.698GluLeu: 6.698 ± 0.067
1.406GluMet: 1.406 ± 0.029
1.584GluAsn: 1.584 ± 0.032
2.445GluPro: 2.445 ± 0.037
3.434GluGln: 3.434 ± 0.05
4.428GluArg: 4.428 ± 0.054
2.747GluSer: 2.747 ± 0.043
2.589GluThr: 2.589 ± 0.039
4.099GluVal: 4.099 ± 0.051
0.76GluTrp: 0.76 ± 0.021
1.241GluTyr: 1.241 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.155PheAla: 4.155 ± 0.049
0.502PheCys: 0.502 ± 0.016
2.569PheAsp: 2.569 ± 0.035
2.151PheGlu: 2.151 ± 0.031
1.606PhePhe: 1.606 ± 0.034
3.402PheGly: 3.402 ± 0.054
0.802PheHis: 0.802 ± 0.022
2.053PheIle: 2.053 ± 0.036
1.438PheLys: 1.438 ± 0.029
3.408PheLeu: 3.408 ± 0.053
0.937PheMet: 0.937 ± 0.022
1.485PheAsn: 1.485 ± 0.033
1.527PhePro: 1.527 ± 0.026
1.245PheGln: 1.245 ± 0.025
1.828PheArg: 1.828 ± 0.031
2.601PheSer: 2.601 ± 0.041
2.037PheThr: 2.037 ± 0.029
2.633PheVal: 2.633 ± 0.043
0.576PheTrp: 0.576 ± 0.019
1.12PheTyr: 1.12 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
7.323GlyAla: 7.323 ± 0.078
0.994GlyCys: 0.994 ± 0.029
3.856GlyAsp: 3.856 ± 0.048
4.41GlyGlu: 4.41 ± 0.043
3.57GlyPhe: 3.57 ± 0.046
5.996GlyGly: 5.996 ± 0.079
1.938GlyHis: 1.938 ± 0.032
4.381GlyIle: 4.381 ± 0.051
3.548GlyLys: 3.548 ± 0.051
9.133GlyLeu: 9.133 ± 0.081
2.347GlyMet: 2.347 ± 0.04
2.431GlyAsn: 2.431 ± 0.039
2.723GlyPro: 2.723 ± 0.04
3.455GlyGln: 3.455 ± 0.047
4.613GlyArg: 4.613 ± 0.051
4.61GlySer: 4.61 ± 0.048
3.946GlyThr: 3.946 ± 0.048
6.089GlyVal: 6.089 ± 0.061
1.368GlyTrp: 1.368 ± 0.029
2.464GlyTyr: 2.464 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.259HisAla: 2.259 ± 0.038
0.324HisCys: 0.324 ± 0.014
1.259HisAsp: 1.259 ± 0.026
1.249HisGlu: 1.249 ± 0.024
1.015HisPhe: 1.015 ± 0.024
1.952HisGly: 1.952 ± 0.034
0.637HisHis: 0.637 ± 0.022
1.04HisIle: 1.04 ± 0.023
0.696HisLys: 0.696 ± 0.016
2.592HisLeu: 2.592 ± 0.041
0.569HisMet: 0.569 ± 0.018
0.655HisAsn: 0.655 ± 0.018
1.459HisPro: 1.459 ± 0.03
0.905HisGln: 0.905 ± 0.024
1.31HisArg: 1.31 ± 0.028
1.334HisSer: 1.334 ± 0.028
1.013HisThr: 1.013 ± 0.021
1.445HisVal: 1.445 ± 0.029
0.446HisTrp: 0.446 ± 0.017
0.809HisTyr: 0.809 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
6.199IleAla: 6.199 ± 0.067
0.57IleCys: 0.57 ± 0.019
3.415IleAsp: 3.415 ± 0.044
3.497IleGlu: 3.497 ± 0.051
1.69IlePhe: 1.69 ± 0.031
4.765IleGly: 4.765 ± 0.055
1.034IleHis: 1.034 ± 0.023
2.268IleIle: 2.268 ± 0.04
1.93IleLys: 1.93 ± 0.037
4.314IleLeu: 4.314 ± 0.052
0.936IleMet: 0.936 ± 0.022
1.872IleAsn: 1.872 ± 0.034
2.395IlePro: 2.395 ± 0.036
1.641IleGln: 1.641 ± 0.036
2.872IleArg: 2.872 ± 0.04
3.207IleSer: 3.207 ± 0.05
2.743IleThr: 2.743 ± 0.037
3.482IleVal: 3.482 ± 0.051
0.534IleTrp: 0.534 ± 0.016
1.221IleTyr: 1.221 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
4.321LysAla: 4.321 ± 0.053
0.224LysCys: 0.224 ± 0.01
1.959LysAsp: 1.959 ± 0.038
1.815LysGlu: 1.815 ± 0.039
0.99LysPhe: 0.99 ± 0.026
2.776LysGly: 2.776 ± 0.041
0.826LysHis: 0.826 ± 0.021
1.813LysIle: 1.813 ± 0.036
1.51LysLys: 1.51 ± 0.039
3.983LysLeu: 3.983 ± 0.052
0.838LysMet: 0.838 ± 0.023
1.187LysAsn: 1.187 ± 0.024
2.145LysPro: 2.145 ± 0.034
1.615LysGln: 1.615 ± 0.032
2.426LysArg: 2.426 ± 0.039
2.053LysSer: 2.053 ± 0.032
2.08LysThr: 2.08 ± 0.039
2.945LysVal: 2.945 ± 0.044
0.429LysTrp: 0.429 ± 0.014
0.813LysTyr: 0.813 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
13.183LeuAla: 13.183 ± 0.1
1.221LeuCys: 1.221 ± 0.026
6.515LeuAsp: 6.515 ± 0.063
6.246LeuGlu: 6.246 ± 0.064
4.21LeuPhe: 4.21 ± 0.057
9.004LeuGly: 9.004 ± 0.09
2.401LeuHis: 2.401 ± 0.037
5.774LeuIle: 5.774 ± 0.058
4.55LeuLys: 4.55 ± 0.049
12.784LeuLeu: 12.784 ± 0.118
2.783LeuMet: 2.783 ± 0.044
3.647LeuAsn: 3.647 ± 0.047
6.06LeuPro: 6.06 ± 0.061
4.436LeuGln: 4.436 ± 0.059
6.813LeuArg: 6.813 ± 0.069
7.295LeuSer: 7.295 ± 0.065
5.97LeuThr: 5.97 ± 0.052
7.533LeuVal: 7.533 ± 0.076
1.344LeuTrp: 1.344 ± 0.032
2.5LeuTyr: 2.5 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
2.781MetAla: 2.781 ± 0.041
0.198MetCys: 0.198 ± 0.01
1.151MetAsp: 1.151 ± 0.026
1.001MetGlu: 1.001 ± 0.024
0.815MetPhe: 0.815 ± 0.023
1.802MetGly: 1.802 ± 0.03
0.52MetHis: 0.52 ± 0.019
1.287MetIle: 1.287 ± 0.027
1.009MetLys: 1.009 ± 0.023
2.834MetLeu: 2.834 ± 0.041
0.561MetMet: 0.561 ± 0.018
0.994MetAsn: 0.994 ± 0.022
1.399MetPro: 1.399 ± 0.029
1.025MetGln: 1.025 ± 0.022
1.557MetArg: 1.557 ± 0.03
1.915MetSer: 1.915 ± 0.026
1.639MetThr: 1.639 ± 0.029
1.654MetVal: 1.654 ± 0.033
0.201MetTrp: 0.201 ± 0.01
0.414MetTyr: 0.414 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.305AsnAla: 3.305 ± 0.044
0.294AsnCys: 0.294 ± 0.012
1.699AsnAsp: 1.699 ± 0.031
1.507AsnGlu: 1.507 ± 0.029
1.142AsnPhe: 1.142 ± 0.023
2.632AsnGly: 2.632 ± 0.043
0.674AsnHis: 0.674 ± 0.018
1.536AsnIle: 1.536 ± 0.029
1.016AsnLys: 1.016 ± 0.026
3.441AsnLeu: 3.441 ± 0.042
0.663AsnMet: 0.663 ± 0.019
1.049AsnAsn: 1.049 ± 0.026
2.082AsnPro: 2.082 ± 0.035
1.256AsnGln: 1.256 ± 0.03
1.931AsnArg: 1.931 ± 0.035
1.701AsnSer: 1.701 ± 0.031
1.61AsnThr: 1.61 ± 0.029
2.108AsnVal: 2.108 ± 0.034
0.493AsnTrp: 0.493 ± 0.016
0.881AsnTyr: 0.881 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
5.298ProAla: 5.298 ± 0.064
0.377ProCys: 0.377 ± 0.015
2.949ProAsp: 2.949 ± 0.034
3.2ProGlu: 3.2 ± 0.046
1.854ProPhe: 1.854 ± 0.032
3.953ProGly: 3.953 ± 0.039
1.09ProHis: 1.09 ± 0.02
2.2ProIle: 2.2 ± 0.037
1.702ProLys: 1.702 ± 0.034
5.506ProLeu: 5.506 ± 0.054
1.231ProMet: 1.231 ± 0.025
1.413ProAsn: 1.413 ± 0.028
1.935ProPro: 1.935 ± 0.042
2.247ProGln: 2.247 ± 0.038
2.471ProArg: 2.471 ± 0.037
2.636ProSer: 2.636 ± 0.039
2.431ProThr: 2.431 ± 0.038
3.952ProVal: 3.952 ± 0.048
0.724ProTrp: 0.724 ± 0.021
1.238ProTyr: 1.238 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.346GlnAla: 5.346 ± 0.062
0.335GlnCys: 0.335 ± 0.014
1.792GlnAsp: 1.792 ± 0.034
1.81GlnGlu: 1.81 ± 0.029
1.362GlnPhe: 1.362 ± 0.027
3.204GlnGly: 3.204 ± 0.043
1.126GlnHis: 1.126 ± 0.025
2.142GlnIle: 2.142 ± 0.036
1.36GlnLys: 1.36 ± 0.026
5.145GlnLeu: 5.145 ± 0.062
1.075GlnMet: 1.075 ± 0.023
1.14GlnAsn: 1.14 ± 0.023
2.299GlnPro: 2.299 ± 0.04
2.431GlnGln: 2.431 ± 0.041
3.26GlnArg: 3.26 ± 0.052
2.295GlnSer: 2.295 ± 0.035
2.083GlnThr: 2.083 ± 0.036
3.418GlnVal: 3.418 ± 0.043
0.733GlnTrp: 0.733 ± 0.02
1.01GlnTyr: 1.01 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
5.599ArgAla: 5.599 ± 0.064
0.581ArgCys: 0.581 ± 0.017
3.303ArgAsp: 3.303 ± 0.043
3.963ArgGlu: 3.963 ± 0.046
2.707ArgPhe: 2.707 ± 0.041
3.881ArgGly: 3.881 ± 0.048
1.656ArgHis: 1.656 ± 0.033
3.339ArgIle: 3.339 ± 0.038
2.24ArgLys: 2.24 ± 0.039
7.673ArgLeu: 7.673 ± 0.072
1.632ArgMet: 1.632 ± 0.028
1.929ArgAsn: 1.929 ± 0.035
2.624ArgPro: 2.624 ± 0.04
3.074ArgGln: 3.074 ± 0.043
3.902ArgArg: 3.902 ± 0.051
3.431ArgSer: 3.431 ± 0.049
2.787ArgThr: 2.787 ± 0.038
4.307ArgVal: 4.307 ± 0.051
1.003ArgTrp: 1.003 ± 0.026
2.035ArgTyr: 2.035 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.164SerAla: 6.164 ± 0.057
0.509SerCys: 0.509 ± 0.017
3.003SerAsp: 3.003 ± 0.042
3.006SerGlu: 3.006 ± 0.039
2.375SerPhe: 2.375 ± 0.032
5.178SerGly: 5.178 ± 0.054
1.334SerHis: 1.334 ± 0.029
3.102SerIle: 3.102 ± 0.046
2.02SerLys: 2.02 ± 0.036
6.855SerLeu: 6.855 ± 0.061
1.5SerMet: 1.5 ± 0.029
1.789SerAsn: 1.789 ± 0.033
2.796SerPro: 2.796 ± 0.038
2.356SerGln: 2.356 ± 0.039
3.65SerArg: 3.65 ± 0.04
3.559SerSer: 3.559 ± 0.051
3.033SerThr: 3.033 ± 0.039
4.227SerVal: 4.227 ± 0.047
0.833SerTrp: 0.833 ± 0.023
1.563SerTyr: 1.563 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
5.319ThrAla: 5.319 ± 0.061
0.517ThrCys: 0.517 ± 0.018
2.635ThrAsp: 2.635 ± 0.04
2.575ThrGlu: 2.575 ± 0.042
1.962ThrPhe: 1.962 ± 0.034
4.365ThrGly: 4.365 ± 0.051
1.162ThrHis: 1.162 ± 0.026
2.207ThrIle: 2.207 ± 0.03
1.262ThrLys: 1.262 ± 0.028
6.638ThrLeu: 6.638 ± 0.07
0.952ThrMet: 0.952 ± 0.021
1.252ThrAsn: 1.252 ± 0.027
3.211ThrPro: 3.211 ± 0.041
1.971ThrGln: 1.971 ± 0.037
3.161ThrArg: 3.161 ± 0.041
2.79ThrSer: 2.79 ± 0.037
2.593ThrThr: 2.593 ± 0.044
3.828ThrVal: 3.828 ± 0.049
0.762ThrTrp: 0.762 ± 0.019
1.287ThrTyr: 1.287 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
7.709ValAla: 7.709 ± 0.061
0.804ValCys: 0.804 ± 0.022
3.962ValAsp: 3.962 ± 0.042
4.325ValGlu: 4.325 ± 0.053
2.713ValPhe: 2.713 ± 0.039
5.352ValGly: 5.352 ± 0.065
1.45ValHis: 1.45 ± 0.024
4.068ValIle: 4.068 ± 0.052
2.528ValLys: 2.528 ± 0.047
8.012ValLeu: 8.012 ± 0.084
1.92ValMet: 1.92 ± 0.03
2.353ValAsn: 2.353 ± 0.038
3.407ValPro: 3.407 ± 0.04
2.631ValGln: 2.631 ± 0.041
4.204ValArg: 4.204 ± 0.047
4.44ValSer: 4.44 ± 0.054
3.83ValThr: 3.83 ± 0.05
5.497ValVal: 5.497 ± 0.059
0.873ValTrp: 0.873 ± 0.024
1.637ValTyr: 1.637 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.193TrpAla: 1.193 ± 0.026
0.177TrpCys: 0.177 ± 0.011
0.644TrpAsp: 0.644 ± 0.021
0.602TrpGlu: 0.602 ± 0.02
0.589TrpPhe: 0.589 ± 0.021
0.968TrpGly: 0.968 ± 0.023
0.389TrpHis: 0.389 ± 0.016
0.707TrpIle: 0.707 ± 0.021
0.54TrpLys: 0.54 ± 0.02
2.117TrpLeu: 2.117 ± 0.041
0.4TrpMet: 0.4 ± 0.014
0.501TrpAsn: 0.501 ± 0.018
0.648TrpPro: 0.648 ± 0.019
0.845TrpGln: 0.845 ± 0.023
1.049TrpArg: 1.049 ± 0.028
0.854TrpSer: 0.854 ± 0.022
0.664TrpThr: 0.664 ± 0.02
0.968TrpVal: 0.968 ± 0.025
0.207TrpTrp: 0.207 ± 0.012
0.355TrpTyr: 0.355 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.52TyrAla: 2.52 ± 0.036
0.339TyrCys: 0.339 ± 0.014
1.391TyrAsp: 1.391 ± 0.033
1.251TyrGlu: 1.251 ± 0.026
1.091TyrPhe: 1.091 ± 0.026
2.126TyrGly: 2.126 ± 0.032
0.591TyrHis: 0.591 ± 0.019
1.107TyrIle: 1.107 ± 0.027
0.875TyrLys: 0.875 ± 0.025
3.092TyrLeu: 3.092 ± 0.038
0.524TyrMet: 0.524 ± 0.015
0.782TyrAsn: 0.782 ± 0.021
1.361TyrPro: 1.361 ± 0.028
1.23TyrGln: 1.23 ± 0.023
1.95TyrArg: 1.95 ± 0.032
1.599TyrSer: 1.599 ± 0.028
1.277TyrThr: 1.277 ± 0.027
1.666TyrVal: 1.666 ± 0.035
0.431TyrTrp: 0.431 ± 0.017
0.748TyrTyr: 0.748 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5946 proteins (1844757 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski