Streptococcus phage phiARI0468-1
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141DZV4|A0A141DZV4_9CAUD RusA domain protein OS=Streptococcus phage phiARI0468-1 OX=1701827 GN=phiARI0468-1_31 PE=4 SV=1
MM1 pKa = 7.54 TKK3 pKa = 10.04 TIEE6 pKa = 4.2 LPDD9 pKa = 3.86 YY10 pKa = 11.11 YY11 pKa = 10.99 EE12 pKa = 4.72 PDD14 pKa = 3.19 WGNARR19 pKa = 11.84 YY20 pKa = 7.53 GTLEE24 pKa = 3.87 EE25 pKa = 4.36 LKK27 pKa = 10.87 EE28 pKa = 3.71 LLLYY32 pKa = 10.33 KK33 pKa = 10.48 RR34 pKa = 11.84 IVEE37 pKa = 3.91 WDD39 pKa = 3.14 KK40 pKa = 11.83 DD41 pKa = 3.83 FLLLEE46 pKa = 5.42 DD47 pKa = 3.96 GTKK50 pKa = 8.98 VTIEE54 pKa = 4.02 MSEE57 pKa = 4.16 SDD59 pKa = 4.01 CCASAGGEE67 pKa = 4.09 FQNVSLDD74 pKa = 3.68 AVITNVEE81 pKa = 3.69 IGEE84 pKa = 4.07 QEE86 pKa = 4.68 EE87 pKa = 5.28 IPDD90 pKa = 3.39 HH91 pKa = 6.31 WGAGYY96 pKa = 10.19 KK97 pKa = 10.47 NKK99 pKa = 8.57 VTIFHH104 pKa = 6.23 NQNPVAIANCEE115 pKa = 4.04 AEE117 pKa = 4.32 HH118 pKa = 6.09 NGYY121 pKa = 9.85 YY122 pKa = 10.37 YY123 pKa = 10.92 SVGSLVIGDD132 pKa = 2.94 IHH134 pKa = 6.99 FPVVHH139 pKa = 6.79 AA140 pKa = 5.01
Molecular weight: 15.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.129
IPC2_protein 4.406
IPC_protein 4.317
Toseland 4.177
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.126
Rodwell 4.164
Grimsley 4.088
Solomon 4.266
Lehninger 4.215
Nozaki 4.38
DTASelect 4.507
Thurlkill 4.177
EMBOSS 4.151
Sillero 4.431
Patrickios 1.163
IPC_peptide 4.266
IPC2_peptide 4.431
IPC2.peptide.svr19 4.326
Protein with the highest isoelectric point:
>tr|A0A141DZU2|A0A141DZU2_9CAUD Phage_Mu_F domain-containing protein OS=Streptococcus phage phiARI0468-1 OX=1701827 GN=phiARI0468-1_18 PE=4 SV=1
MM1 pKa = 7.55 FIWDD5 pKa = 3.59 WVSIAFGWLVFLLLIFIIMAVISGIIKK32 pKa = 9.8 GVKK35 pKa = 9.76 KK36 pKa = 9.73 GTEE39 pKa = 3.21 KK40 pKa = 10.25 WKK42 pKa = 10.77 NGKK45 pKa = 9.27 KK46 pKa = 9.92 DD47 pKa = 3.61 LKK49 pKa = 11.23 KK50 pKa = 10.1 NTTNN54 pKa = 3.39
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.942
IPC2_protein 9.341
IPC_protein 9.238
Toseland 10.555
ProMoST 9.897
Dawson 10.599
Bjellqvist 10.101
Wikipedia 10.643
Rodwell 11.594
Grimsley 10.599
Solomon 10.613
Lehninger 10.613
Nozaki 10.496
DTASelect 10.101
Thurlkill 10.496
EMBOSS 10.891
Sillero 10.496
Patrickios 11.374
IPC_peptide 10.628
IPC2_peptide 8.317
IPC2.peptide.svr19 8.328
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
11277
32
2127
234.9
26.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.855 ± 0.603
0.63 ± 0.114
6.429 ± 0.319
8.158 ± 0.592
4.221 ± 0.271
6.651 ± 0.553
1.215 ± 0.161
6.42 ± 0.308
8.203 ± 0.361
8.096 ± 0.257
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.377 ± 0.186
5.294 ± 0.312
2.758 ± 0.247
4.46 ± 0.267
4.593 ± 0.338
5.977 ± 0.37
6.074 ± 0.411
6.385 ± 0.245
1.392 ± 0.172
3.813 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here