Beihai sobemo-like virus 3
Average proteome isoelectric point is 7.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEB0|A0A1L3KEB0_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 3 OX=1922700 PE=4 SV=1
MM1 pKa = 7.57 GATIAEE7 pKa = 4.36 TLCIVFATLVLTRR20 pKa = 11.84 LSVDD24 pKa = 3.57 YY25 pKa = 10.62 VTDD28 pKa = 4.29 LYY30 pKa = 11.37 GRR32 pKa = 11.84 VYY34 pKa = 10.83 SALAYY39 pKa = 8.48 NAKK42 pKa = 9.91 RR43 pKa = 11.84 VRR45 pKa = 11.84 ACMKK49 pKa = 10.58 LMYY52 pKa = 10.34 CPFRR56 pKa = 11.84 RR57 pKa = 11.84 VEE59 pKa = 4.03 KK60 pKa = 10.5 RR61 pKa = 11.84 DD62 pKa = 3.53 VFPSTEE68 pKa = 4.07 GFFMHH73 pKa = 7.43 PDD75 pKa = 3.15 SSGVLQAYY83 pKa = 9.39 YY84 pKa = 10.42 RR85 pKa = 11.84 FPAEE89 pKa = 3.59 YY90 pKa = 10.59 GGFLQRR96 pKa = 11.84 LEE98 pKa = 4.36 RR99 pKa = 11.84 NGGYY103 pKa = 10.64 GLGGEE108 pKa = 4.65 YY109 pKa = 10.75 VPLWKK114 pKa = 10.23 EE115 pKa = 3.6 AVFNKK120 pKa = 9.81 VGCGEE125 pKa = 4.12 SAIAGAAYY133 pKa = 9.51 EE134 pKa = 4.1 RR135 pKa = 11.84 TVDD138 pKa = 3.39 QKK140 pKa = 11.21 LWKK143 pKa = 9.22 GTFKK147 pKa = 11.31 VNGEE151 pKa = 4.11 TGVYY155 pKa = 10.68 DD156 pKa = 4.55 SMGLNIAGCAVITAHH171 pKa = 7.52 ALLHH175 pKa = 6.22 KK176 pKa = 9.89 EE177 pKa = 3.97 AVVLTGGNTVNVGVRR192 pKa = 11.84 VEE194 pKa = 3.97 TSRR197 pKa = 11.84 FLKK200 pKa = 10.4 PEE202 pKa = 3.45 KK203 pKa = 10.11 CYY205 pKa = 10.38 TGSAFDD211 pKa = 3.88 FAVAPLTANEE221 pKa = 3.91 WSAIGAKK228 pKa = 10.12 ALSSKK233 pKa = 10.71 DD234 pKa = 3.58 FAPVMPGPIDD244 pKa = 3.22 MCFGQGVEE252 pKa = 4.09 GSLFVSKK259 pKa = 10.61 GQLEE263 pKa = 4.4 PQVTRR268 pKa = 11.84 AEE270 pKa = 4.03 KK271 pKa = 10.64 AGIIVASISTEE282 pKa = 3.77 PGASGSVYY290 pKa = 9.7 RR291 pKa = 11.84 RR292 pKa = 11.84 FVGGVPKK299 pKa = 9.84 YY300 pKa = 9.19 TGFHH304 pKa = 5.75 VSRR307 pKa = 11.84 PGEE310 pKa = 3.96 RR311 pKa = 11.84 MSQLVGKK318 pKa = 8.85 YY319 pKa = 10.41 NIGIDD324 pKa = 3.57 FGVIFSFMRR333 pKa = 11.84 KK334 pKa = 8.8 NDD336 pKa = 3.45 LYY338 pKa = 11.31 FDD340 pKa = 3.51 TTYY343 pKa = 11.36 SVLRR347 pKa = 11.84 KK348 pKa = 9.54 LAGLSVGEE356 pKa = 4.2 SFDD359 pKa = 3.79 YY360 pKa = 11.35 DD361 pKa = 3.43 FDD363 pKa = 4.96 KK364 pKa = 11.36 NCKK367 pKa = 9.76 EE368 pKa = 3.89 PLDD371 pKa = 3.77 YY372 pKa = 11.51 DD373 pKa = 4.11 EE374 pKa = 6.0 IEE376 pKa = 4.14 RR377 pKa = 11.84 NYY379 pKa = 10.88 LDD381 pKa = 5.12 SEE383 pKa = 4.54 EE384 pKa = 4.13 MWQTTQDD391 pKa = 4.21 EE392 pKa = 4.6 ITKK395 pKa = 9.48 MIYY398 pKa = 10.04 GDD400 pKa = 3.8 RR401 pKa = 11.84 YY402 pKa = 10.86 DD403 pKa = 4.04 PVDD406 pKa = 3.17 MHH408 pKa = 6.29 VGKK411 pKa = 10.69 RR412 pKa = 11.84 YY413 pKa = 9.72 GRR415 pKa = 11.84 RR416 pKa = 11.84 TRR418 pKa = 11.84 DD419 pKa = 3.41 QMPDD423 pKa = 3.15 EE424 pKa = 4.22 HH425 pKa = 7.13 WYY427 pKa = 10.93 GEE429 pKa = 4.37 SCEE432 pKa = 4.27 PPVLTVAPGLKK443 pKa = 9.53 LDD445 pKa = 4.24 PVIEE449 pKa = 4.38 STDD452 pKa = 3.7 DD453 pKa = 3.44 DD454 pKa = 5.01 CFDD457 pKa = 4.79 DD458 pKa = 4.63 ACSEE462 pKa = 4.43 PGTCDD467 pKa = 3.22 DD468 pKa = 4.45 VAVSSPSFAEE478 pKa = 4.18 DD479 pKa = 4.2 DD480 pKa = 4.18 EE481 pKa = 5.6 LVQCSPKK488 pKa = 10.11 PLSSVIARR496 pKa = 11.84 AAICASAASAVALSLDD512 pKa = 3.41 FSDD515 pKa = 5.01 VKK517 pKa = 10.89 RR518 pKa = 11.84 QVLNSDD524 pKa = 4.08 FSFVGKK530 pKa = 10.27 LDD532 pKa = 3.74 EE533 pKa = 5.53 AVDD536 pKa = 3.84 KK537 pKa = 11.39 YY538 pKa = 11.32 GADD541 pKa = 3.29 AVHH544 pKa = 7.01 EE545 pKa = 4.54 YY546 pKa = 10.78 VVSTPAFATYY556 pKa = 10.19 RR557 pKa = 11.84 DD558 pKa = 3.93 YY559 pKa = 11.81 HH560 pKa = 6.19 NVTMFTEE567 pKa = 5.02 TSSEE571 pKa = 3.93 EE572 pKa = 4.12 TLPDD576 pKa = 3.3 MNGNTFFTKK585 pKa = 9.8 IGEE588 pKa = 3.89 YY589 pKa = 10.31 RR590 pKa = 11.84 IDD592 pKa = 3.75 GKK594 pKa = 10.63 RR595 pKa = 11.84 APTTPEE601 pKa = 3.63 RR602 pKa = 11.84 RR603 pKa = 11.84 KK604 pKa = 10.34 KK605 pKa = 9.91 PPKK608 pKa = 9.83 NLSDD612 pKa = 3.27 AAKK615 pKa = 10.24 ARR617 pKa = 11.84 EE618 pKa = 3.86 AAVRR622 pKa = 11.84 EE623 pKa = 4.29 LIKK626 pKa = 10.87 DD627 pKa = 3.88 LSYY630 pKa = 11.44 DD631 pKa = 3.46 GEE633 pKa = 4.36 DD634 pKa = 2.72 WVTPEE639 pKa = 4.14 NSRR642 pKa = 11.84 KK643 pKa = 10.25 NILDD647 pKa = 3.29 SMRR650 pKa = 11.84 AHH652 pKa = 7.24 AGLACVEE659 pKa = 4.56 SPPATEE665 pKa = 5.21 DD666 pKa = 2.98 DD667 pKa = 3.45 WNRR670 pKa = 11.84 ALEE673 pKa = 4.15 KK674 pKa = 10.71 GMEE677 pKa = 4.2 VFDD680 pKa = 4.28 CSPIEE685 pKa = 3.99 SHH687 pKa = 6.86 AEE689 pKa = 3.57 RR690 pKa = 11.84 GFEE693 pKa = 3.79 GWYY696 pKa = 9.8 KK697 pKa = 10.14 QAATLQDD704 pKa = 3.42 SSSGVSARR712 pKa = 11.84 FRR714 pKa = 11.84 SLSKK718 pKa = 10.47 RR719 pKa = 11.84 QWVQDD724 pKa = 3.46 PQMFQVLIDD733 pKa = 3.96 LVQCRR738 pKa = 11.84 LVLMLIHH745 pKa = 6.55 SRR747 pKa = 11.84 FVQDD751 pKa = 3.5 YY752 pKa = 10.39 SPEE755 pKa = 4.23 TVVKK759 pKa = 10.79 YY760 pKa = 10.48 GLKK763 pKa = 10.34 DD764 pKa = 3.51 VLEE767 pKa = 4.4 VTVKK771 pKa = 10.66 PEE773 pKa = 3.57 SHH775 pKa = 6.53 KK776 pKa = 10.69 PEE778 pKa = 4.08 KK779 pKa = 10.59 AKK781 pKa = 10.6 LGRR784 pKa = 11.84 WRR786 pKa = 11.84 LIWICSVIDD795 pKa = 3.89 CLVQKK800 pKa = 10.44 LLHH803 pKa = 6.3 KK804 pKa = 10.44 AVNARR809 pKa = 11.84 DD810 pKa = 3.47 IEE812 pKa = 4.21 NYY814 pKa = 9.5 QCGKK818 pKa = 7.65 TLHH821 pKa = 6.45 SAAGMGHH828 pKa = 6.63 HH829 pKa = 7.7 DD830 pKa = 3.83 EE831 pKa = 5.81 GIKK834 pKa = 9.44 QLCRR838 pKa = 11.84 TLKK841 pKa = 10.53 EE842 pKa = 4.07 LFGDD846 pKa = 3.44 ATEE849 pKa = 6.25 LITCDD854 pKa = 2.86 ASMWDD859 pKa = 3.52 FTMDD863 pKa = 3.19 KK864 pKa = 10.03 QAHH867 pKa = 5.59 INAAKK872 pKa = 10.25 RR873 pKa = 11.84 RR874 pKa = 11.84 VACCKK879 pKa = 10.03 SEE881 pKa = 3.96 PVARR885 pKa = 11.84 LIMTLGHH892 pKa = 5.83 VNYY895 pKa = 10.51 KK896 pKa = 9.85 HH897 pKa = 6.33 VCEE900 pKa = 4.5 CKK902 pKa = 10.24 GDD904 pKa = 2.89 IWRR907 pKa = 11.84 CEE909 pKa = 3.85 KK910 pKa = 10.9 EE911 pKa = 4.25 GVNGSGQCSTTSDD924 pKa = 3.28 NTFSRR929 pKa = 11.84 VGQARR934 pKa = 11.84 ASGADD939 pKa = 3.12 RR940 pKa = 11.84 VIANGDD946 pKa = 3.47 DD947 pKa = 3.27 MAADD951 pKa = 4.26 LGFDD955 pKa = 3.73 PEE957 pKa = 3.99 AAKK960 pKa = 10.94 AFGTRR965 pKa = 11.84 SRR967 pKa = 11.84 DD968 pKa = 3.59 VVVQDD973 pKa = 3.89 ANLVPFTSHH982 pKa = 6.96 HH983 pKa = 6.31 INRR986 pKa = 11.84 TTCTASYY993 pKa = 10.81 DD994 pKa = 3.93 RR995 pKa = 11.84 PLKK998 pKa = 10.69 LAFNLLSNCRR1008 pKa = 11.84 SEE1010 pKa = 4.44 DD1011 pKa = 2.95 FGMRR1015 pKa = 11.84 VDD1017 pKa = 3.21 ALMYY1021 pKa = 9.49 VVRR1024 pKa = 11.84 NTPDD1028 pKa = 3.05 ALAKK1032 pKa = 10.01 FKK1034 pKa = 10.96 KK1035 pKa = 9.97 HH1036 pKa = 6.22 LGPHH1040 pKa = 6.3 AKK1042 pKa = 8.83 GTDD1045 pKa = 3.58 TCCVEE1050 pKa = 4.41 MLWGAA1055 pKa = 3.62
Molecular weight: 116.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.469
IPC2_protein 5.499
IPC_protein 5.525
Toseland 5.741
ProMoST 5.804
Dawson 5.703
Bjellqvist 5.753
Wikipedia 5.664
Rodwell 5.664
Grimsley 5.792
Solomon 5.703
Lehninger 5.677
Nozaki 5.919
DTASelect 6.109
Thurlkill 6.02
EMBOSS 5.982
Sillero 6.008
Patrickios 3.986
IPC_peptide 5.715
IPC2_peptide 6.008
IPC2.peptide.svr19 6.016
Protein with the highest isoelectric point:
>tr|A0A1L3KEB0|A0A1L3KEB0_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 3 OX=1922700 PE=4 SV=1
MM1 pKa = 7.2 PRR3 pKa = 11.84 RR4 pKa = 11.84 WGRR7 pKa = 11.84 SSPSDD12 pKa = 3.21 AVLVTGVQQGAAAAVPKK29 pKa = 10.77 AFGSTAGRR37 pKa = 11.84 RR38 pKa = 11.84 WGNRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 TSANSRR50 pKa = 11.84 PNNRR54 pKa = 11.84 WQRR57 pKa = 11.84 RR58 pKa = 11.84 KK59 pKa = 9.66 RR60 pKa = 11.84 AGIAEE65 pKa = 4.39 SACWDD70 pKa = 3.61 AFSPVHH76 pKa = 5.97 LALPRR81 pKa = 11.84 AVAPYY86 pKa = 9.03 ATIRR90 pKa = 11.84 TTAIWNPDD98 pKa = 2.64 VDD100 pKa = 4.86 AGDD103 pKa = 3.86 GNKK106 pKa = 9.56 FALFGPYY113 pKa = 10.02 LDD115 pKa = 4.68 GSADD119 pKa = 3.32 AGQWSNLFSLTANTALSNPKK139 pKa = 8.26 NTNGGAKK146 pKa = 9.58 YY147 pKa = 10.38 YY148 pKa = 10.4 AFEE151 pKa = 4.28 SMISGSWKK159 pKa = 9.84 AASVTPAAFSIQIMNPEE176 pKa = 4.17 ALQTSSGAVYY186 pKa = 10.46 IGRR189 pKa = 11.84 CKK191 pKa = 10.75 NRR193 pKa = 11.84 VSVQEE198 pKa = 4.17 GVLTEE203 pKa = 4.28 TWQLLAEE210 pKa = 4.29 EE211 pKa = 4.78 LVSYY215 pKa = 10.19 SNPRR219 pKa = 11.84 ICSAGKK225 pKa = 9.08 LALRR229 pKa = 11.84 GVQIDD234 pKa = 4.76 AIPNNMSEE242 pKa = 4.44 LANFTTLKK250 pKa = 10.05 EE251 pKa = 4.33 YY252 pKa = 10.87 SDD254 pKa = 3.6 HH255 pKa = 5.66 TTNLSSTVPHH265 pKa = 6.8 PVHH268 pKa = 6.79 PSGFNPIFVYY278 pKa = 10.85 NPDD281 pKa = 3.5 GVKK284 pKa = 10.47 LQILVCCEE292 pKa = 3.01 WRR294 pKa = 11.84 VRR296 pKa = 11.84 FDD298 pKa = 3.72 PSNPAYY304 pKa = 10.09 AACSSHH310 pKa = 6.88 KK311 pKa = 9.14 PTSDD315 pKa = 3.67 TTWHH319 pKa = 6.14 KK320 pKa = 10.59 HH321 pKa = 4.15 MQHH324 pKa = 6.5 ALAAGNGVLDD334 pKa = 3.64 IAEE337 pKa = 4.11 KK338 pKa = 8.57 VARR341 pKa = 11.84 YY342 pKa = 8.16 GVPIAKK348 pKa = 10.06 AAGYY352 pKa = 10.32 LGGAA356 pKa = 4.15
Molecular weight: 38.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.897
IPC2_protein 8.916
IPC_protein 8.887
Toseland 9.37
ProMoST 9.268
Dawson 9.706
Bjellqvist 9.531
Wikipedia 9.911
Rodwell 9.882
Grimsley 9.794
Solomon 9.75
Lehninger 9.706
Nozaki 9.619
DTASelect 9.458
Thurlkill 9.56
EMBOSS 9.838
Sillero 9.692
Patrickios 4.8
IPC_peptide 9.75
IPC2_peptide 8.434
IPC2.peptide.svr19 7.816
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1411
356
1055
705.5
77.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.993 ± 1.666
2.693 ± 0.481
6.378 ± 1.302
5.457 ± 1.13
4.04 ± 0.453
7.583 ± 0.135
2.197 ± 0.024
3.614 ± 0.152
5.386 ± 0.694
7.016 ± 0.265
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.268 ± 0.412
3.827 ± 1.123
4.819 ± 0.784
2.693 ± 0.189
5.953 ± 0.108
7.442 ± 0.739
5.67 ± 0.109
7.796 ± 0.637
1.701 ± 0.529
3.473 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here