Streptacidiphilus pinicola
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7437 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2X0IB27|A0A2X0IB27_9ACTN TetR family transcriptional regulator OS=Streptacidiphilus pinicola OX=2219663 GN=DN069_35915 PE=4 SV=1
MM1 pKa = 7.6 IGTLRR6 pKa = 11.84 AYY8 pKa = 10.29 ALDD11 pKa = 4.4 CPDD14 pKa = 4.71 PLALAAFYY22 pKa = 11.36 AEE24 pKa = 4.19 LVGGKK29 pKa = 9.52 VVDD32 pKa = 4.78 EE33 pKa = 4.36 GDD35 pKa = 2.99 GWVEE39 pKa = 4.16 LEE41 pKa = 3.89 GDD43 pKa = 4.17 YY44 pKa = 9.77 GTKK47 pKa = 10.31 LAFQQVEE54 pKa = 4.58 TFHH57 pKa = 7.25 APAWPGQDD65 pKa = 3.07 TPQQAHH71 pKa = 5.42 VDD73 pKa = 3.4 IGVADD78 pKa = 4.87 LDD80 pKa = 3.97 AAQTQVLALGATLLEE95 pKa = 4.83 DD96 pKa = 3.43 WDD98 pKa = 4.31 GTKK101 pKa = 9.6 SWRR104 pKa = 11.84 VFADD108 pKa = 3.57 PAGHH112 pKa = 7.02 PFCLCACC119 pKa = 4.94
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.732
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.304
Thurlkill 3.795
EMBOSS 3.897
Sillero 4.075
Patrickios 0.947
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.947
Protein with the highest isoelectric point:
>tr|A0A2X0J477|A0A2X0J477_9ACTN GNAT family N-acetyltransferase OS=Streptacidiphilus pinicola OX=2219663 GN=DN069_13840 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.51 GRR40 pKa = 11.84 SSLSAA45 pKa = 3.62
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7437
0
7437
2402842
21
5425
323.1
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.972 ± 0.042
0.815 ± 0.007
5.623 ± 0.02
5.282 ± 0.035
2.788 ± 0.014
9.459 ± 0.034
2.322 ± 0.013
3.104 ± 0.021
1.781 ± 0.019
10.755 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.682 ± 0.012
1.901 ± 0.021
6.087 ± 0.026
3.131 ± 0.017
7.676 ± 0.044
5.315 ± 0.027
6.158 ± 0.034
8.523 ± 0.027
1.569 ± 0.012
2.056 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here