Chryseolinea flava

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Fulvivirgaceae; Chryseolinea

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A364Y9H3|A0A364Y9H3_9BACT N-acetylglucosamine kinase OS=Chryseolinea flava OX=2059302 GN=DQQ10_02495 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84FNLIYY7 pKa = 10.47SFAVAALFVCAFCAQPCFGQRR28 pKa = 11.84YY29 pKa = 7.87PLVFNSSSLDD39 pKa = 3.43GSNGFVMKK47 pKa = 10.67GNAANQVLGAEE58 pKa = 4.3LQFVGDD64 pKa = 3.99INNDD68 pKa = 3.35GLEE71 pKa = 4.35DD72 pKa = 3.76LAFSDD77 pKa = 3.99EE78 pKa = 4.17LEE80 pKa = 4.38EE81 pKa = 4.53VGGLALGGRR90 pKa = 11.84AYY92 pKa = 9.72IVFGSSTPFPKK103 pKa = 10.61EE104 pKa = 3.72FDD106 pKa = 3.57PKK108 pKa = 10.15TLNGSNGFMVDD119 pKa = 3.13PTTADD124 pKa = 3.1EE125 pKa = 5.09RR126 pKa = 11.84RR127 pKa = 11.84GQSIAGPGDD136 pKa = 3.43INGDD140 pKa = 3.97GIDD143 pKa = 4.78DD144 pKa = 4.84IIVSATSGEE153 pKa = 4.26LTMVIYY159 pKa = 10.35GRR161 pKa = 11.84SGAFPAVMKK170 pKa = 11.0VGDD173 pKa = 3.76INGTNGFVIQIDD185 pKa = 3.76GTTQVDD191 pKa = 3.51ALGDD195 pKa = 3.8MNGDD199 pKa = 4.55GINDD203 pKa = 3.85FAISNPHH210 pKa = 4.91WSEE213 pKa = 3.84RR214 pKa = 11.84AWVIFGRR221 pKa = 11.84SGNFPASINNTWLDD235 pKa = 3.64GVKK238 pKa = 10.88GFMTNPFPGSRR249 pKa = 11.84PAYY252 pKa = 9.22RR253 pKa = 11.84VGAAGDD259 pKa = 3.96INNDD263 pKa = 3.29GYY265 pKa = 11.65DD266 pKa = 3.82DD267 pKa = 4.84LLVGNWASASAEE279 pKa = 3.91AFSFLLFGRR288 pKa = 11.84ASFTSQVEE296 pKa = 4.31LTTLNGTDD304 pKa = 3.41GFRR307 pKa = 11.84IEE309 pKa = 4.18NAGNNFITCVGRR321 pKa = 11.84VGDD324 pKa = 3.99INGDD328 pKa = 3.51GMDD331 pKa = 4.41DD332 pKa = 3.94CFSEE336 pKa = 4.17NNLIFGSTSPFPSTLSMSSLNGNNGALLDD365 pKa = 4.54GYY367 pKa = 8.83VHH369 pKa = 6.87HH370 pKa = 7.29ACSAGDD376 pKa = 3.69LSGDD380 pKa = 4.1GIDD383 pKa = 4.39DD384 pKa = 4.39LLVVGQTGNYY394 pKa = 7.67VVFGTASGFPALVDD408 pKa = 3.84PEE410 pKa = 6.32DD411 pKa = 3.88IDD413 pKa = 3.79GTNGFTIPTSNTNIGRR429 pKa = 11.84PIDD432 pKa = 3.79GGKK435 pKa = 10.25DD436 pKa = 3.41FNGDD440 pKa = 3.38GFSDD444 pKa = 4.18FAYY447 pKa = 10.72DD448 pKa = 4.03NISGDD453 pKa = 3.39GTVAVVFGGDD463 pKa = 4.31HH464 pKa = 6.76IALPLNNNPSIIQLRR479 pKa = 11.84SDD481 pKa = 3.77GFRR484 pKa = 11.84IRR486 pKa = 11.84FNAKK490 pKa = 6.58EE491 pKa = 4.01TGIIRR496 pKa = 11.84YY497 pKa = 8.4AVYY500 pKa = 9.84PSSTPLMNNLDD511 pKa = 4.11VIDD514 pKa = 4.7NGTGAIVSGSFPIGSSNVDD533 pKa = 3.54IIHH536 pKa = 6.86LLDD539 pKa = 3.95GLAPDD544 pKa = 4.02TEE546 pKa = 4.56YY547 pKa = 11.37DD548 pKa = 3.91VYY550 pKa = 11.96VHH552 pKa = 6.92FLDD555 pKa = 5.09DD556 pKa = 5.44AGNKK560 pKa = 9.38DD561 pKa = 3.39INYY564 pKa = 8.64HH565 pKa = 5.49HH566 pKa = 7.66WDD568 pKa = 3.53NVKK571 pKa = 8.72TLPLGDD577 pKa = 3.78ITPPSITCPGNQTVVCTATTIPDD600 pKa = 3.27YY601 pKa = 10.12TALVTATDD609 pKa = 3.87NTDD612 pKa = 3.19PTPTVTQSPVSGSTFVNGMTITMTATDD639 pKa = 4.33DD640 pKa = 3.71AGNTQTCTFTVTKK653 pKa = 9.87TVDD656 pKa = 3.23AQKK659 pKa = 9.15PTITCPTDD667 pKa = 2.98RR668 pKa = 11.84TLSLNATIPDD678 pKa = 3.96YY679 pKa = 11.08RR680 pKa = 11.84SLATVTDD687 pKa = 3.64NCDD690 pKa = 3.05ASPTVTQSPVAGTIFTGPLTVTLTATDD717 pKa = 3.45ASSNVQTCSFFVGKK731 pKa = 10.37APDD734 pKa = 3.78TAPPTITCSGNQSLSCASTTIPSYY758 pKa = 11.33VSLATVSDD766 pKa = 4.23NEE768 pKa = 4.41DD769 pKa = 3.15ATPTVTQSPAAGSPFVDD786 pKa = 3.45GMTVTLTAIDD796 pKa = 4.13DD797 pKa = 4.12EE798 pKa = 5.49GNVASCNFIVAKK810 pKa = 8.37TTDD813 pKa = 3.42TQKK816 pKa = 11.05PIINCPGDD824 pKa = 3.55QVLTPGFVLPNYY836 pKa = 9.94VPTATTSDD844 pKa = 3.95NCDD847 pKa = 3.2MAPVVTQSPASGTMFITSQVVTLTVTDD874 pKa = 3.8ASGNSEE880 pKa = 3.83SCSFNVTPAPDD891 pKa = 3.63VEE893 pKa = 5.45DD894 pKa = 3.99PTISCPGNQSVSCTAITLPSYY915 pKa = 10.79ISLATVSDD923 pKa = 4.03NQDD926 pKa = 2.81ATPTVTQSPAAGSPVVTGMTVTLTATDD953 pKa = 3.99DD954 pKa = 3.92AGNTATCSFVVSKK967 pKa = 9.61TVDD970 pKa = 3.23AQKK973 pKa = 9.16PTITCPGNKK982 pKa = 9.23VLTIGEE988 pKa = 4.48TLPDD992 pKa = 3.79YY993 pKa = 10.81RR994 pKa = 11.84SLATAVDD1001 pKa = 3.84NCDD1004 pKa = 3.64LAPVITQSPASGGVFMAATVVTLTVTDD1031 pKa = 3.83ASGNSEE1037 pKa = 4.1TCSFTVTPAPDD1048 pKa = 3.52TEE1050 pKa = 4.97APAISCAGNQSVTCMTTSLPNYY1072 pKa = 9.06ISLATVSDD1080 pKa = 4.03NQDD1083 pKa = 2.99ATPTITQSPATGTPITNGMTVTLTATDD1110 pKa = 3.86DD1111 pKa = 3.82AGNSSTCSFMVAKK1124 pKa = 9.76PADD1127 pKa = 3.81TQKK1130 pKa = 11.12PIITCPANTTLNMGDD1145 pKa = 4.66AIADD1149 pKa = 3.72YY1150 pKa = 9.78TSQAIVADD1158 pKa = 4.04NCDD1161 pKa = 3.32ATPTVTQSPIAGTSFTGALTVTLTAKK1187 pKa = 10.26DD1188 pKa = 3.36ASGNTEE1194 pKa = 3.64VCSFSIAEE1202 pKa = 4.55APDD1205 pKa = 3.74SQSPTLTCPGDD1216 pKa = 3.49QLVDD1220 pKa = 3.7CDD1222 pKa = 3.8VTTVADD1228 pKa = 3.65YY1229 pKa = 9.85TSQVVASDD1237 pKa = 3.97NKK1239 pKa = 10.39DD1240 pKa = 3.11ASPTVTQSPAPGTTLTNGMTITLTAADD1267 pKa = 3.38IAGNEE1272 pKa = 4.44TTCSFTINVKK1282 pKa = 10.76SNVRR1286 pKa = 11.84VNAGRR1291 pKa = 11.84DD1292 pKa = 3.34VIMTVGGNVQLSAVASIDD1310 pKa = 3.15NGTFLWTPGEE1320 pKa = 4.11SLDD1323 pKa = 5.46RR1324 pKa = 11.84DD1325 pKa = 4.49DD1326 pKa = 5.22IPNPVATPLATTTYY1340 pKa = 10.03KK1341 pKa = 10.95VLFTTPNGCTATDD1354 pKa = 3.66EE1355 pKa = 4.65VKK1357 pKa = 10.98VEE1359 pKa = 4.2VKK1361 pKa = 10.34DD1362 pKa = 3.37LHH1364 pKa = 7.89IPEE1367 pKa = 4.96GFSPDD1372 pKa = 3.35GDD1374 pKa = 3.49GLNEE1378 pKa = 3.69NWILTGIEE1386 pKa = 4.01KK1387 pKa = 10.93YY1388 pKa = 8.82KK1389 pKa = 10.14TNEE1392 pKa = 3.47VNIFNRR1398 pKa = 11.84WGNCVYY1404 pKa = 10.56SVKK1407 pKa = 10.6GYY1409 pKa = 11.0NNQTLVFNGNANRR1422 pKa = 11.84LSALGADD1429 pKa = 4.0NLPDD1433 pKa = 3.24GTYY1436 pKa = 10.1FYY1438 pKa = 10.94SITLNDD1444 pKa = 3.21SSTPIKK1450 pKa = 10.74GFIVLKK1456 pKa = 10.59RR1457 pKa = 3.57

Molecular weight: 151.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A364XWY4|A0A364XWY4_9BACT Uncharacterized protein OS=Chryseolinea flava OX=2059302 GN=DQQ10_25885 PE=3 SV=1
MM1 pKa = 7.55AKK3 pKa = 10.01YY4 pKa = 9.99GKK6 pKa = 9.64KK7 pKa = 9.89AQSTVRR13 pKa = 11.84SAMKK17 pKa = 9.92RR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 9.86KK21 pKa = 9.44GTLRR25 pKa = 11.84SGSGAKK31 pKa = 8.46VTSRR35 pKa = 11.84KK36 pKa = 8.12QAIAIGLSEE45 pKa = 4.44ARR47 pKa = 11.84QKK49 pKa = 9.87GAKK52 pKa = 9.43VPSKK56 pKa = 9.53KK57 pKa = 8.95TPSKK61 pKa = 9.79TRR63 pKa = 11.84AKK65 pKa = 10.15KK66 pKa = 9.07SARR69 pKa = 11.84RR70 pKa = 11.84KK71 pKa = 5.55PTKK74 pKa = 10.45RR75 pKa = 11.84MAKK78 pKa = 9.64RR79 pKa = 11.84SKK81 pKa = 8.96KK82 pKa = 6.56TARR85 pKa = 11.84AKK87 pKa = 10.44RR88 pKa = 3.58

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5446

0

5446

1947148

33

3382

357.5

40.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.415 ± 0.033

0.759 ± 0.012

5.576 ± 0.025

5.835 ± 0.036

5.058 ± 0.025

6.738 ± 0.035

2.034 ± 0.017

7.04 ± 0.024

6.618 ± 0.04

9.14 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.015

5.219 ± 0.032

3.71 ± 0.023

3.637 ± 0.019

4.352 ± 0.023

6.563 ± 0.029

6.058 ± 0.045

6.855 ± 0.025

1.234 ± 0.012

3.873 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski