Pseudolabrys taiwanensis
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5199 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345ZXA0|A0A345ZXA0_9RHIZ Uncharacterized protein OS=Pseudolabrys taiwanensis OX=331696 GN=DW352_14100 PE=4 SV=1
MM1 pKa = 7.48 TISSVSSAYY10 pKa = 10.13 AAATASTSSTSSTDD24 pKa = 2.76 SSMSLSTTDD33 pKa = 3.86 FLQLIVTQLKK43 pKa = 8.91 NQNPLDD49 pKa = 4.25 PTDD52 pKa = 3.71 PTQFMSQLVSYY63 pKa = 10.9 ASYY66 pKa = 10.8 DD67 pKa = 3.12 QQTQMNSNLTSMVSSMNSLLTSNGIGYY94 pKa = 9.82 IGQTVEE100 pKa = 4.45 AKK102 pKa = 10.8 GDD104 pKa = 3.76 TTPLTGGQANWGYY117 pKa = 10.38 SLSSAAASVNITVKK131 pKa = 10.5 DD132 pKa = 3.64 QNGNAVYY139 pKa = 7.56 TTTGDD144 pKa = 3.39 TAAGSHH150 pKa = 5.5 TFTWDD155 pKa = 2.7 GTTNSGGTAADD166 pKa = 3.25 GDD168 pKa = 4.25 YY169 pKa = 10.25 TISVGAANASGSSVLDD185 pKa = 3.46 YY186 pKa = 8.31 TTVIGKK192 pKa = 7.83 VTGVDD197 pKa = 3.63 STSGTSQLLIGDD209 pKa = 3.89 TTVALTSVVGVKK221 pKa = 9.68 SANN224 pKa = 3.23
Molecular weight: 22.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.503
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.783
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.706
Nozaki 3.897
DTASelect 4.228
Thurlkill 3.592
EMBOSS 3.783
Sillero 3.872
Patrickios 0.54
IPC_peptide 3.745
IPC2_peptide 3.834
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A346A3H3|A0A346A3H3_9RHIZ ABC transporter substrate-binding protein OS=Pseudolabrys taiwanensis OX=331696 GN=DW352_26240 PE=4 SV=1
MM1 pKa = 7.32 VLPSAPPPPPPRR13 pKa = 11.84 SRR15 pKa = 11.84 PPPLPPRR22 pKa = 11.84 SRR24 pKa = 11.84 PPPPRR29 pKa = 11.84 RR30 pKa = 11.84 PQRR33 pKa = 11.84 PRR35 pKa = 11.84 RR36 pKa = 11.84 PSRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 CRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 PRR47 pKa = 11.84 PPQPRR52 pKa = 11.84 PPLPPISYY60 pKa = 10.14 RR61 pKa = 3.5
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.93
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5199
0
5199
1630845
29
6358
313.7
34.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.79 ± 0.048
0.823 ± 0.011
5.501 ± 0.027
5.229 ± 0.035
3.742 ± 0.027
8.385 ± 0.038
1.998 ± 0.017
5.211 ± 0.024
3.851 ± 0.033
9.843 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.463 ± 0.018
2.754 ± 0.023
5.344 ± 0.032
3.131 ± 0.024
6.877 ± 0.038
5.149 ± 0.029
5.505 ± 0.039
7.754 ± 0.029
1.301 ± 0.015
2.349 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here