Synechococcus phage S-CBM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3ULJ7|I3ULJ7_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBM2 OX=351708 GN=SWPG_00113 PE=4 SV=1
MM1 pKa = 6.99KK2 pKa = 9.92TKK4 pKa = 10.79LNWWEE9 pKa = 3.7YY10 pKa = 9.8WIGHH14 pKa = 5.46CWMTGWQSIRR24 pKa = 11.84MTFRR28 pKa = 11.84IWADD32 pKa = 3.23LMTSNYY38 pKa = 10.48DD39 pKa = 3.02GYY41 pKa = 11.87ALLKK45 pKa = 10.44EE46 pKa = 4.74DD47 pKa = 5.12DD48 pKa = 4.65PEE50 pKa = 4.48TEE52 pKa = 4.05CVEE55 pKa = 4.24WFWASLNEE63 pKa = 4.34DD64 pKa = 3.23DD65 pKa = 6.22VYY67 pKa = 10.97PQEE70 pKa = 3.95FLEE73 pKa = 5.14YY74 pKa = 10.25LMQMCEE80 pKa = 4.28DD81 pKa = 3.64IEE83 pKa = 4.34LGKK86 pKa = 10.62VEE88 pKa = 4.72TYY90 pKa = 11.62SMDD93 pKa = 3.24EE94 pKa = 3.88VMDD97 pKa = 4.23RR98 pKa = 11.84LKK100 pKa = 11.25DD101 pKa = 3.71EE102 pKa = 5.04LDD104 pKa = 3.92CEE106 pKa = 4.33EE107 pKa = 4.81LTDD110 pKa = 4.8DD111 pKa = 4.58

Molecular weight: 13.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3ULM1|I3ULM1_9CAUD Baseplate wedge component gp53 OS=Synechococcus phage S-CBM2 OX=351708 GN=SWPG_00137 PE=4 SV=1
MM1 pKa = 7.94KK2 pKa = 10.17IPNWQHH8 pKa = 5.71HH9 pKa = 5.56SKK11 pKa = 10.74KK12 pKa = 8.69EE13 pKa = 4.17QKK15 pKa = 9.99RR16 pKa = 11.84HH17 pKa = 5.78LKK19 pKa = 8.1PQAMRR24 pKa = 11.84QAKK27 pKa = 9.47KK28 pKa = 10.12RR29 pKa = 11.84RR30 pKa = 11.84QSLKK34 pKa = 10.43RR35 pKa = 11.84KK36 pKa = 9.01LLSASVLLVGVTSSPANAITWGEE59 pKa = 3.68FWEE62 pKa = 4.76PFDD65 pKa = 4.0GHH67 pKa = 5.57HH68 pKa = 6.87HH69 pKa = 4.37YY70 pKa = 10.35HH71 pKa = 5.73YY72 pKa = 10.35RR73 pKa = 11.84HH74 pKa = 6.14YY75 pKa = 10.82DD76 pKa = 3.28PRR78 pKa = 11.84PVRR81 pKa = 11.84RR82 pKa = 11.84LCWTEE87 pKa = 3.88VQVEE91 pKa = 4.73RR92 pKa = 11.84YY93 pKa = 10.02LPGDD97 pKa = 3.27RR98 pKa = 11.84WRR100 pKa = 11.84RR101 pKa = 11.84GRR103 pKa = 11.84VVFEE107 pKa = 4.44TKK109 pKa = 8.97WVEE112 pKa = 4.2RR113 pKa = 11.84PCC115 pKa = 5.39

Molecular weight: 14.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

197

0

197

53844

49

4440

273.3

30.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.491 ± 0.259

0.93 ± 0.102

6.368 ± 0.146

6.01 ± 0.328

4.279 ± 0.098

7.862 ± 0.39

1.456 ± 0.119

6.55 ± 0.206

5.724 ± 0.45

7.356 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.198

6.043 ± 0.283

3.898 ± 0.155

3.74 ± 0.117

4.234 ± 0.159

7.264 ± 0.218

7.566 ± 0.454

6.76 ± 0.201

1.25 ± 0.125

4.247 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski