Amino acid dipepetide frequency for Sphingomonas phage PAU

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.015AlaAla: 2.015 ± 0.246
0.461AlaCys: 0.461 ± 0.096
2.145AlaAsp: 2.145 ± 0.176
2.274AlaGlu: 2.274 ± 0.195
2.332AlaPhe: 2.332 ± 0.178
2.044AlaGly: 2.044 ± 0.257
0.662AlaHis: 0.662 ± 0.088
3.901AlaIle: 3.901 ± 0.262
4.203AlaLys: 4.203 ± 0.284
4.505AlaLeu: 4.505 ± 0.346
1.151AlaMet: 1.151 ± 0.134
3.08AlaAsn: 3.08 ± 0.247
1.094AlaPro: 1.094 ± 0.138
1.353AlaGln: 1.353 ± 0.179
1.454AlaArg: 1.454 ± 0.142
2.735AlaSer: 2.735 ± 0.222
2.317AlaThr: 2.317 ± 0.254
2.317AlaVal: 2.317 ± 0.234
0.389AlaTrp: 0.389 ± 0.082
2.245AlaTyr: 2.245 ± 0.18
0.0AlaXaa: 0.0 ± 0.0
Cys
0.288CysAla: 0.288 ± 0.059
0.101CysCys: 0.101 ± 0.045
0.619CysAsp: 0.619 ± 0.1
0.676CysGlu: 0.676 ± 0.101
0.461CysPhe: 0.461 ± 0.083
0.331CysGly: 0.331 ± 0.067
0.288CysHis: 0.288 ± 0.067
0.892CysIle: 0.892 ± 0.123
0.849CysLys: 0.849 ± 0.137
0.648CysLeu: 0.648 ± 0.103
0.259CysMet: 0.259 ± 0.064
0.504CysAsn: 0.504 ± 0.078
0.273CysPro: 0.273 ± 0.054
0.273CysGln: 0.273 ± 0.065
0.302CysArg: 0.302 ± 0.085
0.993CysSer: 0.993 ± 0.168
0.533CysThr: 0.533 ± 0.128
0.633CysVal: 0.633 ± 0.099
0.115CysTrp: 0.115 ± 0.042
0.345CysTyr: 0.345 ± 0.068
0.0CysXaa: 0.0 ± 0.0
Asp
2.663AspAla: 2.663 ± 0.215
0.461AspCys: 0.461 ± 0.101
3.339AspAsp: 3.339 ± 0.228
3.987AspGlu: 3.987 ± 0.262
4.174AspPhe: 4.174 ± 0.257
3.123AspGly: 3.123 ± 0.192
0.936AspHis: 0.936 ± 0.114
6.319AspIle: 6.319 ± 0.283
5.34AspLys: 5.34 ± 0.315
6.592AspLeu: 6.592 ± 0.329
1.598AspMet: 1.598 ± 0.153
4.332AspAsn: 4.332 ± 0.24
1.655AspPro: 1.655 ± 0.159
1.468AspGln: 1.468 ± 0.147
2.36AspArg: 2.36 ± 0.212
5.153AspSer: 5.153 ± 0.248
3.757AspThr: 3.757 ± 0.215
3.627AspVal: 3.627 ± 0.245
0.72AspTrp: 0.72 ± 0.099
3.339AspTyr: 3.339 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
3.929GluAla: 3.929 ± 0.268
0.82GluCys: 0.82 ± 0.116
4.073GluAsp: 4.073 ± 0.297
5.052GluGlu: 5.052 ± 0.488
3.8GluPhe: 3.8 ± 0.241
2.087GluGly: 2.087 ± 0.183
1.612GluHis: 1.612 ± 0.145
6.736GluIle: 6.736 ± 0.369
4.591GluLys: 4.591 ± 0.278
8.348GluLeu: 8.348 ± 0.375
1.785GluMet: 1.785 ± 0.153
4.908GluAsn: 4.908 ± 0.283
1.756GluPro: 1.756 ± 0.171
2.217GluGln: 2.217 ± 0.17
3.023GluArg: 3.023 ± 0.224
5.714GluSer: 5.714 ± 0.278
4.476GluThr: 4.476 ± 0.322
3.555GluVal: 3.555 ± 0.209
0.763GluTrp: 0.763 ± 0.094
3.238GluTyr: 3.238 ± 0.233
0.0GluXaa: 0.0 ± 0.0
Phe
2.044PheAla: 2.044 ± 0.197
0.547PheCys: 0.547 ± 0.091
4.721PheAsp: 4.721 ± 0.304
4.663PheGlu: 4.663 ± 0.261
2.058PhePhe: 2.058 ± 0.177
2.145PheGly: 2.145 ± 0.143
0.921PheHis: 0.921 ± 0.103
3.512PheIle: 3.512 ± 0.25
4.534PheLys: 4.534 ± 0.282
3.382PheLeu: 3.382 ± 0.205
1.439PheMet: 1.439 ± 0.148
4.433PheAsn: 4.433 ± 0.686
1.209PhePro: 1.209 ± 0.143
1.67PheGln: 1.67 ± 0.158
2.188PheArg: 2.188 ± 0.21
3.742PheSer: 3.742 ± 0.232
2.936PheThr: 2.936 ± 0.168
2.922PheVal: 2.922 ± 0.174
0.518PheTrp: 0.518 ± 0.09
2.13PheTyr: 2.13 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
1.9GlyAla: 1.9 ± 0.234
0.547GlyCys: 0.547 ± 0.136
2.375GlyAsp: 2.375 ± 0.209
2.504GlyGlu: 2.504 ± 0.184
2.533GlyPhe: 2.533 ± 0.17
1.986GlyGly: 1.986 ± 0.234
0.734GlyHis: 0.734 ± 0.115
3.641GlyIle: 3.641 ± 0.248
3.771GlyLys: 3.771 ± 0.245
3.857GlyLeu: 3.857 ± 0.298
1.411GlyMet: 1.411 ± 0.166
3.195GlyAsn: 3.195 ± 0.234
0.748GlyPro: 0.748 ± 0.12
1.339GlyGln: 1.339 ± 0.156
1.353GlyArg: 1.353 ± 0.143
3.181GlySer: 3.181 ± 0.191
3.051GlyThr: 3.051 ± 0.271
2.202GlyVal: 2.202 ± 0.192
0.547GlyTrp: 0.547 ± 0.1
2.231GlyTyr: 2.231 ± 0.178
0.0GlyXaa: 0.0 ± 0.0
His
0.748HisAla: 0.748 ± 0.103
0.144HisCys: 0.144 ± 0.043
1.295HisAsp: 1.295 ± 0.151
1.554HisGlu: 1.554 ± 0.156
0.792HisPhe: 0.792 ± 0.084
1.022HisGly: 1.022 ± 0.104
0.432HisHis: 0.432 ± 0.076
1.684HisIle: 1.684 ± 0.127
1.367HisLys: 1.367 ± 0.135
2.116HisLeu: 2.116 ± 0.191
0.461HisMet: 0.461 ± 0.079
1.339HisAsn: 1.339 ± 0.134
0.777HisPro: 0.777 ± 0.116
0.417HisGln: 0.417 ± 0.061
0.518HisArg: 0.518 ± 0.088
1.195HisSer: 1.195 ± 0.126
0.964HisThr: 0.964 ± 0.116
1.123HisVal: 1.123 ± 0.138
0.202HisTrp: 0.202 ± 0.046
0.576HisTyr: 0.576 ± 0.098
0.0HisXaa: 0.0 ± 0.0
Ile
3.613IleAla: 3.613 ± 0.271
0.835IleCys: 0.835 ± 0.114
7.038IleAsp: 7.038 ± 0.359
6.822IleGlu: 6.822 ± 0.401
3.584IlePhe: 3.584 ± 0.248
3.354IleGly: 3.354 ± 0.321
1.554IleHis: 1.554 ± 0.172
5.527IleIle: 5.527 ± 0.297
6.894IleLys: 6.894 ± 0.305
6.131IleLeu: 6.131 ± 0.301
1.67IleMet: 1.67 ± 0.173
5.743IleAsn: 5.743 ± 0.325
2.576IlePro: 2.576 ± 0.186
2.907IleGln: 2.907 ± 0.202
3.008IleArg: 3.008 ± 0.211
6.534IleSer: 6.534 ± 0.307
4.85IleThr: 4.85 ± 0.247
5.397IleVal: 5.397 ± 0.293
0.461IleTrp: 0.461 ± 0.081
2.893IleTyr: 2.893 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
4.16LysAla: 4.16 ± 0.236
0.633LysCys: 0.633 ± 0.095
5.239LysAsp: 5.239 ± 0.266
6.088LysGlu: 6.088 ± 0.374
4.116LysPhe: 4.116 ± 0.241
3.051LysGly: 3.051 ± 0.223
1.828LysHis: 1.828 ± 0.172
6.117LysIle: 6.117 ± 0.259
4.721LysLys: 4.721 ± 0.274
8.406LysLeu: 8.406 ± 0.346
2.101LysMet: 2.101 ± 0.194
5.21LysAsn: 5.21 ± 0.306
2.36LysPro: 2.36 ± 0.207
2.62LysGln: 2.62 ± 0.208
3.8LysArg: 3.8 ± 0.236
6.434LysSer: 6.434 ± 0.408
5.743LysThr: 5.743 ± 0.296
4.707LysVal: 4.707 ± 0.236
0.662LysTrp: 0.662 ± 0.098
3.843LysTyr: 3.843 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
4.577LeuAla: 4.577 ± 0.346
0.734LeuCys: 0.734 ± 0.098
5.872LeuAsp: 5.872 ± 0.283
7.053LeuGlu: 7.053 ± 0.373
4.188LeuPhe: 4.188 ± 0.233
3.67LeuGly: 3.67 ± 0.285
2.13LeuHis: 2.13 ± 0.195
6.722LeuIle: 6.722 ± 0.349
7.456LeuLys: 7.456 ± 0.329
6.578LeuLeu: 6.578 ± 0.324
2.26LeuMet: 2.26 ± 0.172
6.448LeuAsn: 6.448 ± 0.309
2.691LeuPro: 2.691 ± 0.192
3.051LeuGln: 3.051 ± 0.212
3.757LeuArg: 3.757 ± 0.235
7.801LeuSer: 7.801 ± 0.394
6.16LeuThr: 6.16 ± 0.277
4.678LeuVal: 4.678 ± 0.256
0.59LeuTrp: 0.59 ± 0.091
3.987LeuTyr: 3.987 ± 0.252
0.0LeuXaa: 0.0 ± 0.0
Met
0.792MetAla: 0.792 ± 0.126
0.187MetCys: 0.187 ± 0.047
1.281MetAsp: 1.281 ± 0.122
1.655MetGlu: 1.655 ± 0.163
2.029MetPhe: 2.029 ± 0.545
0.777MetGly: 0.777 ± 0.113
0.273MetHis: 0.273 ± 0.062
2.231MetIle: 2.231 ± 0.161
3.109MetLys: 3.109 ± 0.241
2.145MetLeu: 2.145 ± 0.203
0.547MetMet: 0.547 ± 0.105
1.9MetAsn: 1.9 ± 0.156
0.461MetPro: 0.461 ± 0.084
0.792MetGln: 0.792 ± 0.096
0.748MetArg: 0.748 ± 0.108
2.404MetSer: 2.404 ± 0.261
1.799MetThr: 1.799 ± 0.163
0.82MetVal: 0.82 ± 0.102
0.086MetTrp: 0.086 ± 0.034
0.964MetTyr: 0.964 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
3.253AsnAla: 3.253 ± 0.257
0.777AsnCys: 0.777 ± 0.172
4.865AsnAsp: 4.865 ± 0.256
5.498AsnGlu: 5.498 ± 0.277
3.397AsnPhe: 3.397 ± 0.251
3.771AsnGly: 3.771 ± 0.322
1.454AsnHis: 1.454 ± 0.16
5.541AsnIle: 5.541 ± 0.292
4.75AsnLys: 4.75 ± 0.295
6.822AsnLeu: 6.822 ± 0.27
1.842AsnMet: 1.842 ± 0.34
4.174AsnAsn: 4.174 ± 0.283
2.735AsnPro: 2.735 ± 0.172
2.749AsnGln: 2.749 ± 0.336
2.936AsnArg: 2.936 ± 0.228
5.354AsnSer: 5.354 ± 0.368
4.016AsnThr: 4.016 ± 0.316
4.85AsnVal: 4.85 ± 0.344
0.849AsnTrp: 0.849 ± 0.112
3.023AsnTyr: 3.023 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
1.094ProAla: 1.094 ± 0.122
0.216ProCys: 0.216 ± 0.071
1.713ProAsp: 1.713 ± 0.161
2.706ProGlu: 2.706 ± 0.251
1.238ProPhe: 1.238 ± 0.131
1.166ProGly: 1.166 ± 0.147
0.374ProHis: 0.374 ± 0.075
2.029ProIle: 2.029 ± 0.201
2.188ProLys: 2.188 ± 0.18
2.332ProLeu: 2.332 ± 0.207
0.648ProMet: 0.648 ± 0.092
2.001ProAsn: 2.001 ± 0.173
0.648ProPro: 0.648 ± 0.085
0.792ProGln: 0.792 ± 0.13
0.892ProArg: 0.892 ± 0.115
1.914ProSer: 1.914 ± 0.129
1.857ProThr: 1.857 ± 0.163
2.188ProVal: 2.188 ± 0.219
0.245ProTrp: 0.245 ± 0.065
1.209ProTyr: 1.209 ± 0.147
0.0ProXaa: 0.0 ± 0.0
Gln
1.742GlnAla: 1.742 ± 0.178
0.23GlnCys: 0.23 ± 0.065
1.626GlnAsp: 1.626 ± 0.153
1.972GlnGlu: 1.972 ± 0.164
1.842GlnPhe: 1.842 ± 0.154
1.295GlnGly: 1.295 ± 0.169
0.403GlnHis: 0.403 ± 0.084
2.274GlnIle: 2.274 ± 0.167
2.648GlnLys: 2.648 ± 0.182
2.778GlnLeu: 2.778 ± 0.191
0.576GlnMet: 0.576 ± 0.066
2.217GlnAsn: 2.217 ± 0.188
0.806GlnPro: 0.806 ± 0.196
0.835GlnGln: 0.835 ± 0.119
1.151GlnArg: 1.151 ± 0.162
1.828GlnSer: 1.828 ± 0.171
1.67GlnThr: 1.67 ± 0.142
1.857GlnVal: 1.857 ± 0.201
0.317GlnTrp: 0.317 ± 0.067
1.425GlnTyr: 1.425 ± 0.15
0.0GlnXaa: 0.0 ± 0.0
Arg
1.166ArgAla: 1.166 ± 0.141
0.475ArgCys: 0.475 ± 0.102
1.684ArgAsp: 1.684 ± 0.153
2.159ArgGlu: 2.159 ± 0.19
2.476ArgPhe: 2.476 ± 0.207
1.324ArgGly: 1.324 ± 0.141
0.72ArgHis: 0.72 ± 0.122
3.8ArgIle: 3.8 ± 0.257
3.368ArgLys: 3.368 ± 0.223
4.102ArgLeu: 4.102 ± 0.245
1.094ArgMet: 1.094 ± 0.13
3.44ArgAsn: 3.44 ± 0.21
0.878ArgPro: 0.878 ± 0.134
0.907ArgGln: 0.907 ± 0.115
1.511ArgArg: 1.511 ± 0.154
2.274ArgSer: 2.274 ± 0.166
2.504ArgThr: 2.504 ± 0.204
1.655ArgVal: 1.655 ± 0.155
0.374ArgTrp: 0.374 ± 0.073
2.332ArgTyr: 2.332 ± 0.226
0.0ArgXaa: 0.0 ± 0.0
Ser
2.389SerAla: 2.389 ± 0.204
0.763SerCys: 0.763 ± 0.112
5.585SerAsp: 5.585 ± 0.303
5.844SerGlu: 5.844 ± 0.307
3.814SerPhe: 3.814 ± 0.281
3.512SerGly: 3.512 ± 0.262
1.022SerHis: 1.022 ± 0.123
6.966SerIle: 6.966 ± 0.305
7.34SerLys: 7.34 ± 0.366
7.009SerLeu: 7.009 ± 0.334
2.217SerMet: 2.217 ± 0.314
6.103SerAsn: 6.103 ± 0.395
1.626SerPro: 1.626 ± 0.161
1.727SerGln: 1.727 ± 0.187
2.763SerArg: 2.763 ± 0.242
5.153SerSer: 5.153 ± 0.349
4.174SerThr: 4.174 ± 0.283
4.678SerVal: 4.678 ± 0.247
0.561SerTrp: 0.561 ± 0.091
3.411SerTyr: 3.411 ± 0.282
0.0SerXaa: 0.0 ± 0.0
Thr
2.202ThrAla: 2.202 ± 0.228
0.461ThrCys: 0.461 ± 0.077
3.857ThrAsp: 3.857 ± 0.188
4.505ThrGlu: 4.505 ± 0.27
3.426ThrPhe: 3.426 ± 0.237
3.368ThrGly: 3.368 ± 0.253
0.835ThrHis: 0.835 ± 0.116
5.815ThrIle: 5.815 ± 0.302
5.225ThrLys: 5.225 ± 0.296
5.196ThrLeu: 5.196 ± 0.301
1.151ThrMet: 1.151 ± 0.121
4.994ThrAsn: 4.994 ± 0.349
1.67ThrPro: 1.67 ± 0.138
1.238ThrGln: 1.238 ± 0.123
2.231ThrArg: 2.231 ± 0.185
4.519ThrSer: 4.519 ± 0.433
3.296ThrThr: 3.296 ± 0.285
4.304ThrVal: 4.304 ± 0.273
0.734ThrTrp: 0.734 ± 0.103
2.634ThrTyr: 2.634 ± 0.192
0.0ThrXaa: 0.0 ± 0.0
Val
2.001ValAla: 2.001 ± 0.199
0.302ValCys: 0.302 ± 0.07
2.907ValAsp: 2.907 ± 0.183
3.397ValGlu: 3.397 ± 0.207
3.066ValPhe: 3.066 ± 0.257
2.202ValGly: 2.202 ± 0.189
1.18ValHis: 1.18 ± 0.128
4.232ValIle: 4.232 ± 0.243
5.325ValLys: 5.325 ± 0.256
5.009ValLeu: 5.009 ± 0.276
1.454ValMet: 1.454 ± 0.153
4.347ValAsn: 4.347 ± 0.286
1.842ValPro: 1.842 ± 0.195
1.885ValGln: 1.885 ± 0.223
2.044ValArg: 2.044 ± 0.214
5.455ValSer: 5.455 ± 0.33
4.548ValThr: 4.548 ± 0.404
2.605ValVal: 2.605 ± 0.168
0.633ValTrp: 0.633 ± 0.094
2.936ValTyr: 2.936 ± 0.189
0.0ValXaa: 0.0 ± 0.0
Trp
0.389TrpAla: 0.389 ± 0.072
0.086TrpCys: 0.086 ± 0.033
0.705TrpAsp: 0.705 ± 0.099
0.489TrpGlu: 0.489 ± 0.078
0.518TrpPhe: 0.518 ± 0.088
0.561TrpGly: 0.561 ± 0.088
0.245TrpHis: 0.245 ± 0.054
0.648TrpIle: 0.648 ± 0.094
0.907TrpLys: 0.907 ± 0.106
0.849TrpLeu: 0.849 ± 0.093
0.202TrpMet: 0.202 ± 0.051
0.979TrpAsn: 0.979 ± 0.165
0.043TrpPro: 0.043 ± 0.023
0.23TrpGln: 0.23 ± 0.058
0.302TrpArg: 0.302 ± 0.078
0.792TrpSer: 0.792 ± 0.115
0.417TrpThr: 0.417 ± 0.069
0.518TrpVal: 0.518 ± 0.089
0.072TrpTrp: 0.072 ± 0.035
0.446TrpTyr: 0.446 ± 0.08
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.713TyrAla: 1.713 ± 0.171
0.605TyrCys: 0.605 ± 0.096
4.001TyrAsp: 4.001 ± 0.217
3.584TyrGlu: 3.584 ± 0.279
1.871TyrPhe: 1.871 ± 0.188
2.317TyrGly: 2.317 ± 0.183
1.123TyrHis: 1.123 ± 0.126
2.807TyrIle: 2.807 ± 0.229
3.253TyrLys: 3.253 ± 0.204
3.613TyrLeu: 3.613 ± 0.238
1.123TyrMet: 1.123 ± 0.131
3.31TyrAsn: 3.31 ± 0.222
1.598TyrPro: 1.598 ± 0.144
1.022TyrGln: 1.022 ± 0.108
1.9TyrArg: 1.9 ± 0.216
3.483TyrSer: 3.483 ± 0.245
2.591TyrThr: 2.591 ± 0.227
2.735TyrVal: 2.735 ± 0.214
0.561TyrTrp: 0.561 ± 0.088
2.202TyrTyr: 2.202 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 295 proteins (69479 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski