Ruegeria phage DSS3-P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097EVU9|A0A097EVU9_9CAUD Uncharacterized protein OS=Ruegeria phage DSS3-P1 OX=1555208 GN=DSS3P1_14 PE=4 SV=1
MM1 pKa = 7.46SNTYY5 pKa = 8.72TVAVVEE11 pKa = 4.9GSTPLPPCYY20 pKa = 9.19PDD22 pKa = 3.63PLLYY26 pKa = 10.62VVTVSDD32 pKa = 3.7PHH34 pKa = 8.21DD35 pKa = 4.06SEE37 pKa = 6.28SILDD41 pKa = 3.7QVAEE45 pKa = 4.07ARR47 pKa = 11.84ALEE50 pKa = 4.07LLGEE54 pKa = 4.49PEE56 pKa = 5.59DD57 pKa = 4.07YY58 pKa = 10.73TPQTFDD64 pKa = 3.39ALRR67 pKa = 11.84AGLRR71 pKa = 11.84LLFVLAGDD79 pKa = 4.45VYY81 pKa = 10.73PVADD85 pKa = 4.78FRR87 pKa = 11.84QSS89 pKa = 2.73

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097EW06|A0A097EW06_9CAUD Uncharacterized protein OS=Ruegeria phage DSS3-P1 OX=1555208 GN=DSS3P1_10 PE=4 SV=1
MM1 pKa = 7.75PPSQIFQNSDD11 pKa = 3.34YY12 pKa = 10.76VVAVEE17 pKa = 4.14GLEE20 pKa = 3.91GLAVDD25 pKa = 4.8FAEE28 pKa = 4.18ITDD31 pKa = 5.04RR32 pKa = 11.84IAEE35 pKa = 4.21RR36 pKa = 11.84ASMAINTTARR46 pKa = 11.84RR47 pKa = 11.84YY48 pKa = 7.34RR49 pKa = 11.84TTSSRR54 pKa = 11.84LIRR57 pKa = 11.84DD58 pKa = 3.26QVAFPARR65 pKa = 11.84YY66 pKa = 9.59LDD68 pKa = 3.8AQANGALRR76 pKa = 11.84VRR78 pKa = 11.84QQATAAKK85 pKa = 9.71LEE87 pKa = 3.97AAIEE91 pKa = 4.25GRR93 pKa = 11.84FDD95 pKa = 3.43PTSLARR101 pKa = 11.84FAKK104 pKa = 9.1GTVAHH109 pKa = 6.37GRR111 pKa = 11.84KK112 pKa = 9.43APRR115 pKa = 11.84LRR117 pKa = 11.84VNPGRR122 pKa = 11.84TTQIPNSFIMNLRR135 pKa = 11.84NGNRR139 pKa = 11.84GLAIRR144 pKa = 11.84LKK146 pKa = 10.44PGEE149 pKa = 4.22TIQNKK154 pKa = 8.83RR155 pKa = 11.84RR156 pKa = 11.84MVSFSRR162 pKa = 11.84KK163 pKa = 9.66DD164 pKa = 3.28SNLYY168 pKa = 10.21LLYY171 pKa = 10.56GPSVDD176 pKa = 3.52QVFRR180 pKa = 11.84SVALDD185 pKa = 3.54VAPDD189 pKa = 3.39AASFLEE195 pKa = 4.38TEE197 pKa = 5.55FIRR200 pKa = 11.84LTEE203 pKa = 3.9NLII206 pKa = 3.93

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

17943

31

1744

218.8

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.704 ± 0.613

0.83 ± 0.121

6.705 ± 0.294

7.279 ± 0.322

3.439 ± 0.132

8.12 ± 0.255

1.984 ± 0.176

4.436 ± 0.146

4.002 ± 0.316

8.789 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.13

3.138 ± 0.156

5.027 ± 0.418

3.361 ± 0.289

7.156 ± 0.309

4.904 ± 0.171

5.902 ± 0.308

6.716 ± 0.198

1.672 ± 0.167

2.586 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski