Bosea sp. AAP35

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1LFH5|A0A0N1LFH5_9BRAD Phosphoenolpyruvate carboxykinase (ATP) OS=Bosea sp. AAP35 OX=1523417 GN=pckA PE=3 SV=1
MM1 pKa = 7.06QALPVPSLLNDD12 pKa = 3.52YY13 pKa = 10.35LPLVIFIGVAAFIGLALLVAPFAIAYY39 pKa = 7.78SNPDD43 pKa = 3.58PEE45 pKa = 4.58KK46 pKa = 10.89LSAYY50 pKa = 9.36EE51 pKa = 4.12CGFNAFDD58 pKa = 4.17DD59 pKa = 4.03ARR61 pKa = 11.84MKK63 pKa = 10.76FDD65 pKa = 3.12VRR67 pKa = 11.84FYY69 pKa = 11.17LVAILFIIFDD79 pKa = 4.21LEE81 pKa = 4.33VAFLFPWAVAFGGLGWYY98 pKa = 8.43GFWSMMLFLGVLTVGFVYY116 pKa = 10.34EE117 pKa = 4.02WRR119 pKa = 11.84KK120 pKa = 9.56GALEE124 pKa = 3.81WDD126 pKa = 3.56

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1B1Z4|A0A0N1B1Z4_9BRAD 2-nitropropane dioxygenase OS=Bosea sp. AAP35 OX=1523417 GN=IP69_18155 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84ARR4 pKa = 11.84TSASQAFGSTSLRR17 pKa = 11.84RR18 pKa = 11.84AVAMSVSMTAARR30 pKa = 11.84SAPRR34 pKa = 11.84WLPPKK39 pKa = 9.62VQLRR43 pKa = 11.84RR44 pKa = 11.84PRR46 pKa = 11.84AKK48 pKa = 9.98PRR50 pKa = 11.84SALSAALLLRR60 pKa = 11.84QILPSSRR67 pKa = 11.84KK68 pKa = 8.54RR69 pKa = 11.84AKK71 pKa = 9.66SAQRR75 pKa = 11.84LSMM78 pKa = 4.34

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3974

0

3974

1218785

30

2139

306.7

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.539 ± 0.055

0.803 ± 0.01

5.473 ± 0.029

5.456 ± 0.036

3.639 ± 0.023

8.847 ± 0.035

1.945 ± 0.019

5.233 ± 0.023

3.105 ± 0.033

10.493 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.016

2.207 ± 0.022

5.337 ± 0.033

3.075 ± 0.021

7.248 ± 0.041

5.143 ± 0.025

5.243 ± 0.026

7.496 ± 0.032

1.296 ± 0.017

1.98 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski