Macaca fascicularis papillomavirus 6
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3PU82|C3PU82_RHPV1 Replication protein E1 OS=Macaca fascicularis papillomavirus 6 OX=471184 GN=E1 PE=3 SV=1
MM1 pKa = 7.69 IGPKK5 pKa = 8.62 PTLQDD10 pKa = 2.85 IVLTSQPEE18 pKa = 4.12 PQPEE22 pKa = 4.11 PVDD25 pKa = 4.12 LMCHH29 pKa = 5.08 EE30 pKa = 5.06 HH31 pKa = 7.51 LSDD34 pKa = 3.65 SSEE37 pKa = 4.57 DD38 pKa = 3.52 EE39 pKa = 4.94 DD40 pKa = 5.92 EE41 pKa = 4.62 IDD43 pKa = 2.92 HH44 pKa = 6.75 HH45 pKa = 8.28 YY46 pKa = 10.79 EE47 pKa = 4.28 DD48 pKa = 5.1 RR49 pKa = 11.84 LEE51 pKa = 4.09 EE52 pKa = 3.92 QQLYY56 pKa = 10.49 RR57 pKa = 11.84 ILSACAHH64 pKa = 6.66 CGSAVRR70 pKa = 11.84 LVVSSTHH77 pKa = 6.91 SEE79 pKa = 4.24 VQVLQQLLMGNLEE92 pKa = 4.31 IVCPSCAGRR101 pKa = 11.84 AA102 pKa = 3.49
Molecular weight: 11.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.237
IPC2_protein 4.495
IPC_protein 4.393
Toseland 4.253
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.215
Rodwell 4.24
Grimsley 4.177
Solomon 4.342
Lehninger 4.291
Nozaki 4.457
DTASelect 4.596
Thurlkill 4.266
EMBOSS 4.24
Sillero 4.52
Patrickios 1.074
IPC_peptide 4.342
IPC2_peptide 4.507
IPC2.peptide.svr19 4.398
Protein with the highest isoelectric point:
>tr|C3PU86|C3PU86_RHPV1 Minor capsid protein L2 OS=Macaca fascicularis papillomavirus 6 OX=471184 GN=L2 PE=3 SV=1
TT1 pKa = 7.36 AKK3 pKa = 10.11 ILLFIVIFLFLFFLGLVVCARR24 pKa = 11.84 LEE26 pKa = 4.16 SLVLCLCVYY35 pKa = 11.04 AKK37 pKa = 10.78 VLLLMLLFWVTIVPPFSAFALCFVCFLLPLLFIHH71 pKa = 6.52 LHH73 pKa = 5.63 ALSVVNSRR81 pKa = 11.84 QQ82 pKa = 3.09
Molecular weight: 9.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.908
IPC2_protein 7.702
IPC_protein 7.688
Toseland 7.132
ProMoST 8.112
Dawson 8.273
Bjellqvist 8.77
Wikipedia 8.185
Rodwell 8.273
Grimsley 7.088
Solomon 8.448
Lehninger 8.478
Nozaki 9.18
DTASelect 8.404
Thurlkill 8.448
EMBOSS 8.507
Sillero 8.843
Patrickios 0.846
IPC_peptide 8.434
IPC2_peptide 8.565
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2394
82
631
299.3
33.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.475 ± 0.514
2.799 ± 0.621
5.556 ± 0.681
5.639 ± 0.831
3.759 ± 0.524
6.14 ± 0.468
2.966 ± 0.369
3.926 ± 0.507
5.18 ± 1.1
9.607 ± 0.998
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.796 ± 0.292
3.342 ± 0.695
6.6 ± 1.384
4.553 ± 0.41
5.263 ± 0.515
7.435 ± 0.41
6.266 ± 0.947
7.686 ± 0.534
1.378 ± 0.384
3.634 ± 0.432
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here