Amino acid dipepetide frequency for Arthrobacter phage Amigo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.913AlaAla: 12.913 ± 2.596
0.404AlaCys: 0.404 ± 0.191
6.399AlaAsp: 6.399 ± 0.765
5.938AlaGlu: 5.938 ± 0.692
2.536AlaPhe: 2.536 ± 0.463
7.898AlaGly: 7.898 ± 0.848
2.191AlaHis: 2.191 ± 0.462
5.073AlaIle: 5.073 ± 0.577
5.822AlaLys: 5.822 ± 0.776
8.935AlaLeu: 8.935 ± 0.73
2.594AlaMet: 2.594 ± 0.437
3.978AlaAsn: 3.978 ± 0.589
3.459AlaPro: 3.459 ± 0.44
4.093AlaGln: 4.093 ± 0.62
4.9AlaArg: 4.9 ± 0.531
5.822AlaSer: 5.822 ± 0.598
5.938AlaThr: 5.938 ± 0.69
6.111AlaVal: 6.111 ± 0.568
1.384AlaTrp: 1.384 ± 0.292
2.767AlaTyr: 2.767 ± 0.332
0.0AlaXaa: 0.0 ± 0.0
Cys
0.576CysAla: 0.576 ± 0.181
0.115CysCys: 0.115 ± 0.084
0.346CysAsp: 0.346 ± 0.143
0.346CysGlu: 0.346 ± 0.164
0.173CysPhe: 0.173 ± 0.103
0.749CysGly: 0.749 ± 0.247
0.173CysHis: 0.173 ± 0.096
0.173CysIle: 0.173 ± 0.095
0.346CysLys: 0.346 ± 0.151
0.461CysLeu: 0.461 ± 0.141
0.058CysMet: 0.058 ± 0.059
0.231CysAsn: 0.231 ± 0.106
0.634CysPro: 0.634 ± 0.173
0.404CysGln: 0.404 ± 0.161
0.404CysArg: 0.404 ± 0.152
0.576CysSer: 0.576 ± 0.216
0.346CysThr: 0.346 ± 0.146
0.461CysVal: 0.461 ± 0.214
0.115CysTrp: 0.115 ± 0.079
0.346CysTyr: 0.346 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
5.822AspAla: 5.822 ± 0.574
0.404AspCys: 0.404 ± 0.162
3.516AspAsp: 3.516 ± 0.43
4.151AspGlu: 4.151 ± 0.615
2.825AspPhe: 2.825 ± 0.388
4.439AspGly: 4.439 ± 0.481
0.576AspHis: 0.576 ± 0.236
3.401AspIle: 3.401 ± 0.433
3.689AspLys: 3.689 ± 0.52
4.842AspLeu: 4.842 ± 0.521
1.326AspMet: 1.326 ± 0.271
2.652AspAsn: 2.652 ± 0.469
2.709AspPro: 2.709 ± 0.417
1.384AspGln: 1.384 ± 0.314
3.747AspArg: 3.747 ± 0.495
3.459AspSer: 3.459 ± 0.513
3.689AspThr: 3.689 ± 0.393
3.574AspVal: 3.574 ± 0.443
1.326AspTrp: 1.326 ± 0.292
3.171AspTyr: 3.171 ± 0.5
0.0AspXaa: 0.0 ± 0.0
Glu
7.609GluAla: 7.609 ± 0.915
0.404GluCys: 0.404 ± 0.126
4.669GluAsp: 4.669 ± 0.624
4.266GluGlu: 4.266 ± 0.657
3.574GluPhe: 3.574 ± 0.535
4.612GluGly: 4.612 ± 0.605
1.441GluHis: 1.441 ± 0.458
3.862GluIle: 3.862 ± 0.484
3.689GluLys: 3.689 ± 0.525
7.148GluLeu: 7.148 ± 0.702
1.441GluMet: 1.441 ± 0.355
2.536GluAsn: 2.536 ± 0.324
2.536GluPro: 2.536 ± 0.408
2.882GluGln: 2.882 ± 0.391
4.093GluArg: 4.093 ± 0.638
3.516GluSer: 3.516 ± 0.526
4.035GluThr: 4.035 ± 0.579
5.246GluVal: 5.246 ± 0.689
1.326GluTrp: 1.326 ± 0.331
2.306GluTyr: 2.306 ± 0.42
0.0GluXaa: 0.0 ± 0.0
Phe
2.652PheAla: 2.652 ± 0.406
0.404PheCys: 0.404 ± 0.149
3.113PheAsp: 3.113 ± 0.431
2.075PheGlu: 2.075 ± 0.392
1.326PhePhe: 1.326 ± 0.212
2.825PheGly: 2.825 ± 0.333
0.922PheHis: 0.922 ± 0.231
1.729PheIle: 1.729 ± 0.321
1.499PheLys: 1.499 ± 0.243
2.075PheLeu: 2.075 ± 0.268
0.98PheMet: 0.98 ± 0.312
1.556PheAsn: 1.556 ± 0.332
1.038PhePro: 1.038 ± 0.265
1.614PheGln: 1.614 ± 0.308
1.672PheArg: 1.672 ± 0.304
2.652PheSer: 2.652 ± 0.342
2.652PheThr: 2.652 ± 0.329
3.286PheVal: 3.286 ± 0.457
0.231PheTrp: 0.231 ± 0.118
0.749PheTyr: 0.749 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
6.514GlyAla: 6.514 ± 0.827
0.519GlyCys: 0.519 ± 0.223
4.439GlyAsp: 4.439 ± 0.514
4.554GlyGlu: 4.554 ± 0.364
3.401GlyPhe: 3.401 ± 0.397
5.592GlyGly: 5.592 ± 0.592
1.326GlyHis: 1.326 ± 0.249
3.228GlyIle: 3.228 ± 0.447
4.669GlyLys: 4.669 ± 0.572
5.822GlyLeu: 5.822 ± 0.518
1.787GlyMet: 1.787 ± 0.32
4.093GlyAsn: 4.093 ± 0.69
2.536GlyPro: 2.536 ± 0.47
2.767GlyGln: 2.767 ± 0.332
2.998GlyArg: 2.998 ± 0.454
5.073GlySer: 5.073 ± 0.624
5.995GlyThr: 5.995 ± 0.607
6.975GlyVal: 6.975 ± 0.562
1.556GlyTrp: 1.556 ± 0.338
3.055GlyTyr: 3.055 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
1.268HisAla: 1.268 ± 0.257
0.231HisCys: 0.231 ± 0.111
0.98HisAsp: 0.98 ± 0.265
1.326HisGlu: 1.326 ± 0.266
0.576HisPhe: 0.576 ± 0.155
1.268HisGly: 1.268 ± 0.266
0.461HisHis: 0.461 ± 0.186
1.095HisIle: 1.095 ± 0.279
1.038HisLys: 1.038 ± 0.292
1.556HisLeu: 1.556 ± 0.277
0.519HisMet: 0.519 ± 0.157
0.576HisAsn: 0.576 ± 0.163
1.153HisPro: 1.153 ± 0.215
0.461HisGln: 0.461 ± 0.152
1.268HisArg: 1.268 ± 0.31
1.038HisSer: 1.038 ± 0.238
1.211HisThr: 1.211 ± 0.25
1.614HisVal: 1.614 ± 0.369
0.461HisTrp: 0.461 ± 0.152
0.461HisTyr: 0.461 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
4.9IleAla: 4.9 ± 0.504
0.519IleCys: 0.519 ± 0.161
3.805IleAsp: 3.805 ± 0.407
4.612IleGlu: 4.612 ± 0.614
1.326IlePhe: 1.326 ± 0.332
3.689IleGly: 3.689 ± 0.618
0.519IleHis: 0.519 ± 0.218
2.364IleIle: 2.364 ± 0.469
2.767IleLys: 2.767 ± 0.401
2.536IleLeu: 2.536 ± 0.345
1.038IleMet: 1.038 ± 0.254
2.133IleAsn: 2.133 ± 0.375
2.191IlePro: 2.191 ± 0.348
1.902IleGln: 1.902 ± 0.47
2.825IleArg: 2.825 ± 0.35
2.998IleSer: 2.998 ± 0.398
3.344IleThr: 3.344 ± 0.403
3.632IleVal: 3.632 ± 0.422
1.095IleTrp: 1.095 ± 0.262
1.902IleTyr: 1.902 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
6.284LysAla: 6.284 ± 0.625
0.231LysCys: 0.231 ± 0.118
2.364LysAsp: 2.364 ± 0.337
3.747LysGlu: 3.747 ± 0.516
1.614LysPhe: 1.614 ± 0.309
3.689LysGly: 3.689 ± 0.336
1.672LysHis: 1.672 ± 0.384
2.882LysIle: 2.882 ± 0.38
2.94LysLys: 2.94 ± 0.388
5.015LysLeu: 5.015 ± 0.497
1.499LysMet: 1.499 ± 0.29
2.536LysAsn: 2.536 ± 0.433
1.845LysPro: 1.845 ± 0.285
2.421LysGln: 2.421 ± 0.503
2.709LysArg: 2.709 ± 0.441
2.998LysSer: 2.998 ± 0.442
4.093LysThr: 4.093 ± 0.393
3.92LysVal: 3.92 ± 0.529
0.922LysTrp: 0.922 ± 0.24
1.326LysTyr: 1.326 ± 0.254
0.0LysXaa: 0.0 ± 0.0
Leu
8.359LeuAla: 8.359 ± 0.803
0.288LeuCys: 0.288 ± 0.117
4.727LeuAsp: 4.727 ± 0.473
5.88LeuGlu: 5.88 ± 0.706
2.133LeuPhe: 2.133 ± 0.312
5.592LeuGly: 5.592 ± 0.626
1.845LeuHis: 1.845 ± 0.347
3.92LeuIle: 3.92 ± 0.392
3.805LeuLys: 3.805 ± 0.393
5.592LeuLeu: 5.592 ± 0.562
1.96LeuMet: 1.96 ± 0.301
3.286LeuAsn: 3.286 ± 0.47
3.516LeuPro: 3.516 ± 0.46
3.055LeuGln: 3.055 ± 0.445
4.266LeuArg: 4.266 ± 0.505
4.727LeuSer: 4.727 ± 0.551
5.822LeuThr: 5.822 ± 0.648
5.188LeuVal: 5.188 ± 0.476
1.384LeuTrp: 1.384 ± 0.353
2.018LeuTyr: 2.018 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
2.594MetAla: 2.594 ± 0.476
0.288MetCys: 0.288 ± 0.129
0.749MetAsp: 0.749 ± 0.194
1.614MetGlu: 1.614 ± 0.312
0.865MetPhe: 0.865 ± 0.191
1.729MetGly: 1.729 ± 0.289
0.461MetHis: 0.461 ± 0.175
0.922MetIle: 0.922 ± 0.228
1.095MetLys: 1.095 ± 0.245
1.441MetLeu: 1.441 ± 0.302
0.461MetMet: 0.461 ± 0.183
0.807MetAsn: 0.807 ± 0.172
1.211MetPro: 1.211 ± 0.316
0.922MetGln: 0.922 ± 0.277
2.075MetArg: 2.075 ± 0.397
1.96MetSer: 1.96 ± 0.361
1.556MetThr: 1.556 ± 0.325
1.499MetVal: 1.499 ± 0.244
0.231MetTrp: 0.231 ± 0.115
0.749MetTyr: 0.749 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
4.093AsnAla: 4.093 ± 0.487
0.173AsnCys: 0.173 ± 0.102
2.652AsnAsp: 2.652 ± 0.363
2.825AsnGlu: 2.825 ± 0.429
1.038AsnPhe: 1.038 ± 0.225
3.805AsnGly: 3.805 ± 0.412
0.519AsnHis: 0.519 ± 0.161
2.018AsnIle: 2.018 ± 0.346
2.133AsnLys: 2.133 ± 0.449
3.401AsnLeu: 3.401 ± 0.541
0.98AsnMet: 0.98 ± 0.235
2.075AsnAsn: 2.075 ± 0.416
2.709AsnPro: 2.709 ± 0.369
1.268AsnGln: 1.268 ± 0.349
2.479AsnArg: 2.479 ± 0.328
2.709AsnSer: 2.709 ± 0.447
2.133AsnThr: 2.133 ± 0.334
3.632AsnVal: 3.632 ± 0.649
0.519AsnTrp: 0.519 ± 0.152
2.018AsnTyr: 2.018 ± 0.525
0.0AsnXaa: 0.0 ± 0.0
Pro
3.459ProAla: 3.459 ± 0.407
0.346ProCys: 0.346 ± 0.169
2.94ProAsp: 2.94 ± 0.514
5.015ProGlu: 5.015 ± 0.465
1.441ProPhe: 1.441 ± 0.278
2.075ProGly: 2.075 ± 0.393
0.692ProHis: 0.692 ± 0.227
1.614ProIle: 1.614 ± 0.276
2.479ProLys: 2.479 ± 0.346
2.882ProLeu: 2.882 ± 0.367
1.038ProMet: 1.038 ± 0.242
1.787ProAsn: 1.787 ± 0.323
1.614ProPro: 1.614 ± 0.337
1.038ProGln: 1.038 ± 0.26
1.96ProArg: 1.96 ± 0.349
2.709ProSer: 2.709 ± 0.384
2.825ProThr: 2.825 ± 0.425
3.171ProVal: 3.171 ± 0.453
0.807ProTrp: 0.807 ± 0.263
1.787ProTyr: 1.787 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
3.805GlnAla: 3.805 ± 0.503
0.461GlnCys: 0.461 ± 0.164
1.96GlnAsp: 1.96 ± 0.336
3.113GlnGlu: 3.113 ± 0.47
1.729GlnPhe: 1.729 ± 0.338
2.479GlnGly: 2.479 ± 0.34
0.576GlnHis: 0.576 ± 0.165
1.845GlnIle: 1.845 ± 0.363
1.672GlnLys: 1.672 ± 0.275
2.767GlnLeu: 2.767 ± 0.348
0.922GlnMet: 0.922 ± 0.194
1.556GlnAsn: 1.556 ± 0.352
0.865GlnPro: 0.865 ± 0.207
2.075GlnGln: 2.075 ± 0.398
1.845GlnArg: 1.845 ± 0.373
2.536GlnSer: 2.536 ± 0.427
2.364GlnThr: 2.364 ± 0.408
2.479GlnVal: 2.479 ± 0.342
0.865GlnTrp: 0.865 ± 0.263
1.384GlnTyr: 1.384 ± 0.274
0.0GlnXaa: 0.0 ± 0.0
Arg
4.151ArgAla: 4.151 ± 0.57
0.288ArgCys: 0.288 ± 0.159
3.344ArgAsp: 3.344 ± 0.439
3.344ArgGlu: 3.344 ± 0.53
1.326ArgPhe: 1.326 ± 0.244
3.92ArgGly: 3.92 ± 0.559
1.038ArgHis: 1.038 ± 0.199
3.401ArgIle: 3.401 ± 0.527
3.344ArgLys: 3.344 ± 0.489
4.612ArgLeu: 4.612 ± 0.522
1.441ArgMet: 1.441 ± 0.232
2.248ArgAsn: 2.248 ± 0.378
2.594ArgPro: 2.594 ± 0.541
2.652ArgGln: 2.652 ± 0.451
2.536ArgArg: 2.536 ± 0.412
2.421ArgSer: 2.421 ± 0.34
2.998ArgThr: 2.998 ± 0.357
4.669ArgVal: 4.669 ± 0.581
0.807ArgTrp: 0.807 ± 0.197
1.672ArgTyr: 1.672 ± 0.299
0.0ArgXaa: 0.0 ± 0.0
Ser
7.033SerAla: 7.033 ± 0.747
0.461SerCys: 0.461 ± 0.153
4.554SerAsp: 4.554 ± 0.479
4.324SerGlu: 4.324 ± 0.711
2.594SerPhe: 2.594 ± 0.348
5.707SerGly: 5.707 ± 0.835
0.865SerHis: 0.865 ± 0.213
3.113SerIle: 3.113 ± 0.36
3.113SerLys: 3.113 ± 0.365
4.439SerLeu: 4.439 ± 0.495
1.787SerMet: 1.787 ± 0.252
2.594SerAsn: 2.594 ± 0.4
1.902SerPro: 1.902 ± 0.371
1.902SerGln: 1.902 ± 0.393
2.191SerArg: 2.191 ± 0.456
4.554SerSer: 4.554 ± 0.875
3.286SerThr: 3.286 ± 0.482
3.574SerVal: 3.574 ± 0.417
0.749SerTrp: 0.749 ± 0.239
1.902SerTyr: 1.902 ± 0.338
0.0SerXaa: 0.0 ± 0.0
Thr
6.341ThrAla: 6.341 ± 0.741
0.346ThrCys: 0.346 ± 0.153
3.113ThrAsp: 3.113 ± 0.381
4.612ThrGlu: 4.612 ± 0.428
2.825ThrPhe: 2.825 ± 0.439
6.687ThrGly: 6.687 ± 0.708
1.384ThrHis: 1.384 ± 0.291
3.632ThrIle: 3.632 ± 0.481
3.401ThrLys: 3.401 ± 0.459
5.073ThrLeu: 5.073 ± 0.561
0.865ThrMet: 0.865 ± 0.261
3.171ThrAsn: 3.171 ± 0.534
3.055ThrPro: 3.055 ± 0.464
2.191ThrGln: 2.191 ± 0.338
3.689ThrArg: 3.689 ± 0.454
3.747ThrSer: 3.747 ± 0.537
4.439ThrThr: 4.439 ± 0.6
5.246ThrVal: 5.246 ± 0.703
0.865ThrTrp: 0.865 ± 0.246
1.96ThrTyr: 1.96 ± 0.33
0.0ThrXaa: 0.0 ± 0.0
Val
6.399ValAla: 6.399 ± 0.62
0.807ValCys: 0.807 ± 0.22
3.805ValAsp: 3.805 ± 0.414
6.226ValGlu: 6.226 ± 0.722
1.614ValPhe: 1.614 ± 0.312
6.111ValGly: 6.111 ± 0.637
1.038ValHis: 1.038 ± 0.24
3.286ValIle: 3.286 ± 0.442
4.669ValLys: 4.669 ± 0.516
4.785ValLeu: 4.785 ± 0.572
1.614ValMet: 1.614 ± 0.261
3.055ValAsn: 3.055 ± 0.455
3.747ValPro: 3.747 ± 0.42
2.421ValGln: 2.421 ± 0.367
3.862ValArg: 3.862 ± 0.683
4.785ValSer: 4.785 ± 0.592
6.053ValThr: 6.053 ± 0.605
4.9ValVal: 4.9 ± 0.486
1.384ValTrp: 1.384 ± 0.339
2.364ValTyr: 2.364 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
1.787TrpAla: 1.787 ± 0.343
0.115TrpCys: 0.115 ± 0.074
1.211TrpAsp: 1.211 ± 0.234
1.326TrpGlu: 1.326 ± 0.266
0.576TrpPhe: 0.576 ± 0.183
1.095TrpGly: 1.095 ± 0.251
0.231TrpHis: 0.231 ± 0.109
0.634TrpIle: 0.634 ± 0.192
0.692TrpLys: 0.692 ± 0.238
1.556TrpLeu: 1.556 ± 0.367
0.231TrpMet: 0.231 ± 0.124
0.576TrpAsn: 0.576 ± 0.161
0.922TrpPro: 0.922 ± 0.221
0.749TrpGln: 0.749 ± 0.241
1.326TrpArg: 1.326 ± 0.303
0.749TrpSer: 0.749 ± 0.204
0.865TrpThr: 0.865 ± 0.2
1.384TrpVal: 1.384 ± 0.325
0.634TrpTrp: 0.634 ± 0.187
0.634TrpTyr: 0.634 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.594TyrAla: 2.594 ± 0.543
0.231TyrCys: 0.231 ± 0.112
2.018TyrAsp: 2.018 ± 0.283
1.902TyrGlu: 1.902 ± 0.397
1.556TyrPhe: 1.556 ± 0.331
2.94TyrGly: 2.94 ± 0.436
0.634TyrHis: 0.634 ± 0.194
1.902TyrIle: 1.902 ± 0.328
1.845TyrLys: 1.845 ± 0.3
2.479TyrLeu: 2.479 ± 0.384
0.634TyrMet: 0.634 ± 0.234
1.729TyrAsn: 1.729 ± 0.274
1.441TyrPro: 1.441 ± 0.283
1.095TyrGln: 1.095 ± 0.239
1.96TyrArg: 1.96 ± 0.35
1.614TyrSer: 1.614 ± 0.254
2.998TyrThr: 2.998 ± 0.317
2.364TyrVal: 2.364 ± 0.407
0.634TyrTrp: 0.634 ± 0.208
0.98TyrTyr: 0.98 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (17348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski