Hondaea fermentalgiana

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Bigyra; Labyrinthulomycetes; Thraustochytrida; Thraustochytriaceae; Hondaea

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11849 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R5G8Q5|A0A2R5G8Q5_9STRA Uncharacterized protein OS=Hondaea fermentalgiana OX=2315210 GN=FCC1311_002622 PE=4 SV=1
MM1 pKa = 6.84FRR3 pKa = 11.84KK4 pKa = 9.32VALVAVLAAACTSAQAQDD22 pKa = 3.4NSDD25 pKa = 3.49WANDD29 pKa = 3.79TMWWDD34 pKa = 3.81DD35 pKa = 4.19DD36 pKa = 4.0EE37 pKa = 5.09PNPVGMLQDD46 pKa = 3.48GDD48 pKa = 4.46LEE50 pKa = 4.45VMYY53 pKa = 10.32EE54 pKa = 3.85ILDD57 pKa = 3.61NDD59 pKa = 4.3MVRR62 pKa = 11.84MTLTYY67 pKa = 7.28PTPNGWIGVGLSDD80 pKa = 4.85DD81 pKa = 3.71GGMVGSHH88 pKa = 5.61AVIGGTGVTGLPAPVGEE105 pKa = 4.62YY106 pKa = 9.86PLEE109 pKa = 4.69SKK111 pKa = 10.92SEE113 pKa = 4.16TPTLYY118 pKa = 10.75SGTNGIYY125 pKa = 8.3NTSVDD130 pKa = 3.74VQDD133 pKa = 4.57SVMTVQFTVEE143 pKa = 4.37SIAGRR148 pKa = 11.84SLDD151 pKa = 4.03LEE153 pKa = 4.64GNGDD157 pKa = 3.7QIIYY161 pKa = 10.29AVYY164 pKa = 10.24RR165 pKa = 11.84GSTWGGAHH173 pKa = 5.74QEE175 pKa = 4.24FGSAMVDD182 pKa = 2.54WSMATPAPTDD192 pKa = 3.59MPSTASRR199 pKa = 11.84AATLSLAAAGMLIGALYY216 pKa = 10.66

Molecular weight: 22.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R5FYP0|A0A2R5FYP0_9STRA CaiB/baiF CoA-transferase family protein DDB_G0269880 OS=Hondaea fermentalgiana OX=2315210 GN=FCC1311_010401 PE=3 SV=1
EEE2 pKa = 4.37RR3 pKa = 11.84AQRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84GLPPAALGRR18 pKa = 11.84CGRR21 pKa = 11.84APRR24 pKa = 11.84AGRR27 pKa = 11.84LRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84AGQRR35 pKa = 11.84HHH37 pKa = 6.18GQALPRR43 pKa = 11.84ALPRR47 pKa = 11.84AARR50 pKa = 11.84DDD52 pKa = 3.17AGAHHH57 pKa = 5.92PRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84AHHH64 pKa = 6.72GAEEE68 pKa = 3.53ALRR71 pKa = 11.84RR72 pKa = 11.84GARR75 pKa = 11.84LLGRR79 pKa = 11.84PRR81 pKa = 11.84GGRR84 pKa = 11.84RR85 pKa = 11.84LRR87 pKa = 11.84ARR89 pKa = 11.84APRR92 pKa = 11.84PAAPAARR99 pKa = 11.84GLRR102 pKa = 11.84ARR104 pKa = 11.84GHHH107 pKa = 6.8GLGLPPRR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84CARR119 pKa = 11.84ARR121 pKa = 11.84GRR123 pKa = 11.84GSGVLV

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11849

0

11849

7193445

22

16137

607.1

66.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.64 ± 0.025

1.481 ± 0.011

6.396 ± 0.019

7.16 ± 0.024

3.562 ± 0.014

6.776 ± 0.031

2.318 ± 0.01

3.865 ± 0.013

4.528 ± 0.025

9.347 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.009

3.252 ± 0.011

4.51 ± 0.017

4.049 ± 0.017

6.782 ± 0.025

7.923 ± 0.03

5.216 ± 0.022

6.721 ± 0.019

1.133 ± 0.007

2.12 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski