Amino acid dipepetide frequency for Paenibacillus donghaensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.132AlaAla: 10.132 ± 0.124	0.733AlaCys: 0.733 ± 0.02	4.447AlaAsp: 4.447 ± 0.052	6.159AlaGlu: 6.159 ± 0.061	3.16AlaPhe: 3.16 ± 0.04	7.238AlaGly: 7.238 ± 0.084	1.387AlaHis: 1.387 ± 0.032	4.913AlaIle: 4.913 ± 0.058	3.96AlaLys: 3.96 ± 0.047	8.394AlaLeu: 8.394 ± 0.069	2.143AlaMet: 2.143 ± 0.031	2.475AlaAsn: 2.475 ± 0.04	2.848AlaPro: 2.848 ± 0.047	2.816AlaGln: 2.816 ± 0.038	3.553AlaArg: 3.553 ± 0.044	4.946AlaSer: 4.946 ± 0.055	3.668AlaThr: 3.668 ± 0.051	6.729AlaVal: 6.729 ± 0.071	0.94AlaTrp: 0.94 ± 0.024	2.801AlaTyr: 2.801 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.56CysAla: 0.56 ± 0.019	0.139CysCys: 0.139 ± 0.009	0.401CysAsp: 0.401 ± 0.013	0.442CysGlu: 0.442 ± 0.013	0.34CysPhe: 0.34 ± 0.011	0.832CysGly: 0.832 ± 0.019	0.182CysHis: 0.182 ± 0.008	0.529CysIle: 0.529 ± 0.014	0.325CysLys: 0.325 ± 0.013	0.815CysLeu: 0.815 ± 0.021	0.214CysMet: 0.214 ± 0.009	0.29CysAsn: 0.29 ± 0.012	0.331CysPro: 0.331 ± 0.012	0.237CysGln: 0.237 ± 0.011	0.52CysArg: 0.52 ± 0.015	0.64CysSer: 0.64 ± 0.017	0.405CysThr: 0.405 ± 0.014	0.417CysVal: 0.417 ± 0.013	0.119CysTrp: 0.119 ± 0.007	0.306CysTyr: 0.306 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.626AspAla: 3.626 ± 0.044	0.398AspCys: 0.398 ± 0.013	2.237AspAsp: 2.237 ± 0.04	3.626AspGlu: 3.626 ± 0.041	2.202AspPhe: 2.202 ± 0.031	3.895AspGly: 3.895 ± 0.046	1.045AspHis: 1.045 ± 0.021	3.609AspIle: 3.609 ± 0.046	2.81AspLys: 2.81 ± 0.039	4.753AspLeu: 4.753 ± 0.048	1.356AspMet: 1.356 ± 0.027	2.052AspAsn: 2.052 ± 0.032	2.243AspPro: 2.243 ± 0.036	1.853AspGln: 1.853 ± 0.029	2.492AspArg: 2.492 ± 0.034	3.081AspSer: 3.081 ± 0.036	2.656AspThr: 2.656 ± 0.039	3.157AspVal: 3.157 ± 0.04	0.811AspTrp: 0.811 ± 0.02	2.294AspTyr: 2.294 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	6.072GluAla: 6.072 ± 0.069	0.454GluCys: 0.454 ± 0.015	3.3GluAsp: 3.3 ± 0.04	5.325GluGlu: 5.325 ± 0.063	2.259GluPhe: 2.259 ± 0.036	4.558GluGly: 4.558 ± 0.055	1.524GluHis: 1.524 ± 0.027	4.454GluIle: 4.454 ± 0.047	3.45GluLys: 3.45 ± 0.044	7.648GluLeu: 7.648 ± 0.077	2.031GluMet: 2.031 ± 0.03	2.492GluAsn: 2.492 ± 0.035	2.299GluPro: 2.299 ± 0.039	3.893GluGln: 3.893 ± 0.044	3.76GluArg: 3.76 ± 0.048	3.497GluSer: 3.497 ± 0.042	3.244GluThr: 3.244 ± 0.036	4.496GluVal: 4.496 ± 0.052	0.934GluTrp: 0.934 ± 0.025	2.236GluTyr: 2.236 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.174PheAla: 3.174 ± 0.044	0.379PheCys: 0.379 ± 0.012	2.152PheAsp: 2.152 ± 0.034	2.316PheGlu: 2.316 ± 0.036	1.785PhePhe: 1.785 ± 0.033	3.049PheGly: 3.049 ± 0.043	0.826PheHis: 0.826 ± 0.02	2.805PheIle: 2.805 ± 0.04	2.051PheLys: 2.051 ± 0.027	3.779PheLeu: 3.779 ± 0.045	1.17PheMet: 1.17 ± 0.026	1.7PheAsn: 1.7 ± 0.029	1.537PhePro: 1.537 ± 0.028	1.364PheGln: 1.364 ± 0.023	1.979PheArg: 1.979 ± 0.029	3.025PheSer: 3.025 ± 0.041	2.527PheThr: 2.527 ± 0.035	2.702PheVal: 2.702 ± 0.035	0.54PheTrp: 0.54 ± 0.016	1.547PheTyr: 1.547 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	5.495GlyAla: 5.495 ± 0.073	0.726GlyCys: 0.726 ± 0.019	3.462GlyAsp: 3.462 ± 0.043	4.748GlyGlu: 4.748 ± 0.05	3.13GlyPhe: 3.13 ± 0.037	5.703GlyGly: 5.703 ± 0.071	1.438GlyHis: 1.438 ± 0.026	5.528GlyIle: 5.528 ± 0.063	4.188GlyLys: 4.188 ± 0.052	7.633GlyLeu: 7.633 ± 0.076	2.359GlyMet: 2.359 ± 0.031	2.887GlyAsn: 2.887 ± 0.047	1.895GlyPro: 1.895 ± 0.031	2.799GlyGln: 2.799 ± 0.039	3.64GlyArg: 3.64 ± 0.045	4.935GlySer: 4.935 ± 0.055	4.381GlyThr: 4.381 ± 0.064	5.082GlyVal: 5.082 ± 0.052	1.072GlyTrp: 1.072 ± 0.025	3.057GlyTyr: 3.057 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	1.378HisAla: 1.378 ± 0.025	0.197HisCys: 0.197 ± 0.01	0.916HisAsp: 0.916 ± 0.022	1.259HisGlu: 1.259 ± 0.026	0.972HisPhe: 0.972 ± 0.019	1.425HisGly: 1.425 ± 0.025	0.556HisHis: 0.556 ± 0.021	1.309HisIle: 1.309 ± 0.027	0.901HisLys: 0.901 ± 0.019	2.042HisLeu: 2.042 ± 0.06	0.526HisMet: 0.526 ± 0.016	0.755HisAsn: 0.755 ± 0.019	1.175HisPro: 1.175 ± 0.024	0.759HisGln: 0.759 ± 0.019	1.03HisArg: 1.03 ± 0.024	1.327HisSer: 1.327 ± 0.023	1.095HisThr: 1.095 ± 0.026	1.254HisVal: 1.254 ± 0.025	0.302HisTrp: 0.302 ± 0.012	0.885HisTyr: 0.885 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.527IleAla: 5.527 ± 0.06	0.625IleCys: 0.625 ± 0.015	3.403IleAsp: 3.403 ± 0.043	4.124IleGlu: 4.124 ± 0.053	2.409IlePhe: 2.409 ± 0.037	4.874IleGly: 4.874 ± 0.056	1.435IleHis: 1.435 ± 0.025	4.285IleIle: 4.285 ± 0.047	3.092IleLys: 3.092 ± 0.041	6.033IleLeu: 6.033 ± 0.062	1.664IleMet: 1.664 ± 0.029	2.602IleAsn: 2.602 ± 0.041	3.083IlePro: 3.083 ± 0.04	2.406IleGln: 2.406 ± 0.034	3.518IleArg: 3.518 ± 0.045	4.773IleSer: 4.773 ± 0.056	4.112IleThr: 4.112 ± 0.041	4.696IleVal: 4.696 ± 0.051	0.667IleTrp: 0.667 ± 0.019	2.26IleTyr: 2.26 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.044LysAla: 4.044 ± 0.057	0.271LysCys: 0.271 ± 0.012	2.874LysAsp: 2.874 ± 0.041	4.164LysGlu: 4.164 ± 0.049	1.546LysPhe: 1.546 ± 0.028	3.492LysGly: 3.492 ± 0.048	1.009LysHis: 1.009 ± 0.022	3.018LysIle: 3.018 ± 0.044	3.047LysLys: 3.047 ± 0.047	5.665LysLeu: 5.665 ± 0.061	1.48LysMet: 1.48 ± 0.024	2.14LysAsn: 2.14 ± 0.031	2.111LysPro: 2.111 ± 0.032	2.526LysGln: 2.526 ± 0.033	2.634LysArg: 2.634 ± 0.038	2.94LysSer: 2.94 ± 0.041	2.67LysThr: 2.67 ± 0.044	3.634LysVal: 3.634 ± 0.047	0.718LysTrp: 0.718 ± 0.02	1.873LysTyr: 1.873 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	8.676LeuAla: 8.676 ± 0.091	0.913LeuCys: 0.913 ± 0.022	5.257LeuAsp: 5.257 ± 0.05	6.674LeuGlu: 6.674 ± 0.061	4.501LeuPhe: 4.501 ± 0.055	6.893LeuGly: 6.893 ± 0.067	2.114LeuHis: 2.114 ± 0.032	6.52LeuIle: 6.52 ± 0.064	5.557LeuLys: 5.557 ± 0.056	12.271LeuLeu: 12.271 ± 0.12	2.724LeuMet: 2.724 ± 0.036	4.168LeuAsn: 4.168 ± 0.048	4.747LeuPro: 4.747 ± 0.052	4.559LeuGln: 4.559 ± 0.051	5.274LeuArg: 5.274 ± 0.061	7.603LeuSer: 7.603 ± 0.056	5.752LeuThr: 5.752 ± 0.053	6.345LeuVal: 6.345 ± 0.064	1.109LeuTrp: 1.109 ± 0.026	3.489LeuTyr: 3.489 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.135MetAla: 2.135 ± 0.031	0.166MetCys: 0.166 ± 0.009	1.531MetAsp: 1.531 ± 0.025	1.862MetGlu: 1.862 ± 0.034	1.028MetPhe: 1.028 ± 0.022	1.744MetGly: 1.744 ± 0.029	0.442MetHis: 0.442 ± 0.014	1.926MetIle: 1.926 ± 0.031	1.913MetLys: 1.913 ± 0.031	3.053MetLeu: 3.053 ± 0.038	0.815MetMet: 0.815 ± 0.02	1.552MetAsn: 1.552 ± 0.026	1.073MetPro: 1.073 ± 0.022	1.014MetGln: 1.014 ± 0.022	1.254MetArg: 1.254 ± 0.025	1.782MetSer: 1.782 ± 0.028	1.508MetThr: 1.508 ± 0.028	1.747MetVal: 1.747 ± 0.032	0.234MetTrp: 0.234 ± 0.01	0.805MetTyr: 0.805 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.822AsnAla: 2.822 ± 0.039	0.296AsnCys: 0.296 ± 0.014	1.877AsnAsp: 1.877 ± 0.029	2.575AsnGlu: 2.575 ± 0.041	1.417AsnPhe: 1.417 ± 0.025	3.201AsnGly: 3.201 ± 0.044	0.846AsnHis: 0.846 ± 0.021	2.58AsnIle: 2.58 ± 0.037	2.227AsnLys: 2.227 ± 0.042	3.607AsnLeu: 3.607 ± 0.045	1.044AsnMet: 1.044 ± 0.022	1.858AsnAsn: 1.858 ± 0.036	1.996AsnPro: 1.996 ± 0.03	1.538AsnGln: 1.538 ± 0.028	2.031AsnArg: 2.031 ± 0.034	2.417AsnSer: 2.417 ± 0.037	2.167AsnThr: 2.167 ± 0.033	2.495AsnVal: 2.495 ± 0.039	0.525AsnTrp: 0.525 ± 0.017	1.524AsnTyr: 1.524 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	3.826ProAla: 3.826 ± 0.057	0.248ProCys: 0.248 ± 0.011	2.407ProAsp: 2.407 ± 0.035	3.443ProGlu: 3.443 ± 0.043	1.783ProPhe: 1.783 ± 0.027	3.061ProGly: 3.061 ± 0.044	0.814ProHis: 0.814 ± 0.02	2.096ProIle: 2.096 ± 0.034	1.666ProLys: 1.666 ± 0.029	4.23ProLeu: 4.23 ± 0.056	0.912ProMet: 0.912 ± 0.02	1.309ProAsn: 1.309 ± 0.022	1.302ProPro: 1.302 ± 0.026	1.701ProGln: 1.701 ± 0.033	1.411ProArg: 1.411 ± 0.028	2.445ProSer: 2.445 ± 0.032	1.882ProThr: 1.882 ± 0.04	3.35ProVal: 3.35 ± 0.041	0.504ProTrp: 0.504 ± 0.017	1.491ProTyr: 1.491 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.531GlnAla: 3.531 ± 0.046	0.225GlnCys: 0.225 ± 0.011	1.799GlnAsp: 1.799 ± 0.03	2.9GlnGlu: 2.9 ± 0.04	1.508GlnPhe: 1.508 ± 0.024	2.767GlnGly: 2.767 ± 0.04	0.844GlnHis: 0.844 ± 0.021	2.489GlnIle: 2.489 ± 0.039	1.939GlnLys: 1.939 ± 0.031	4.626GlnLeu: 4.626 ± 0.051	1.156GlnMet: 1.156 ± 0.021	1.381GlnAsn: 1.381 ± 0.027	1.684GlnPro: 1.684 ± 0.031	2.174GlnGln: 2.174 ± 0.041	2.038GlnArg: 2.038 ± 0.036	2.368GlnSer: 2.368 ± 0.034	2.067GlnThr: 2.067 ± 0.031	2.571GlnVal: 2.571 ± 0.036	0.551GlnTrp: 0.551 ± 0.016	1.432GlnTyr: 1.432 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	3.242ArgAla: 3.242 ± 0.046	0.383ArgCys: 0.383 ± 0.013	2.32ArgAsp: 2.32 ± 0.035	3.684ArgGlu: 3.684 ± 0.048	2.081ArgPhe: 2.081 ± 0.036	2.952ArgGly: 2.952 ± 0.04	1.026ArgHis: 1.026 ± 0.026	3.698ArgIle: 3.698 ± 0.044	2.943ArgLys: 2.943 ± 0.041	5.429ArgLeu: 5.429 ± 0.059	1.595ArgMet: 1.595 ± 0.03	2.093ArgAsn: 2.093 ± 0.031	1.634ArgPro: 1.634 ± 0.028	2.163ArgGln: 2.163 ± 0.035	2.869ArgArg: 2.869 ± 0.049	3.299ArgSer: 3.299 ± 0.044	2.661ArgThr: 2.661 ± 0.034	3.031ArgVal: 3.031 ± 0.039	0.644ArgTrp: 0.644 ± 0.015	1.948ArgTyr: 1.948 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	5.259SerAla: 5.259 ± 0.054	0.469SerCys: 0.469 ± 0.015	2.953SerAsp: 2.953 ± 0.039	3.863SerGlu: 3.863 ± 0.048	3.017SerPhe: 3.017 ± 0.036	5.971SerGly: 5.971 ± 0.059	1.205SerHis: 1.205 ± 0.026	4.379SerIle: 4.379 ± 0.053	3.285SerLys: 3.285 ± 0.041	6.871SerLeu: 6.871 ± 0.057	1.789SerMet: 1.789 ± 0.028	2.376SerAsn: 2.376 ± 0.035	2.621SerPro: 2.621 ± 0.034	2.141SerGln: 2.141 ± 0.029	3.395SerArg: 3.395 ± 0.044	4.646SerSer: 4.646 ± 0.056	3.242SerThr: 3.242 ± 0.042	4.499SerVal: 4.499 ± 0.048	0.859SerTrp: 0.859 ± 0.022	2.448SerTyr: 2.448 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	5.253ThrAla: 5.253 ± 0.067	0.373ThrCys: 0.373 ± 0.014	2.779ThrAsp: 2.779 ± 0.041	3.259ThrGlu: 3.259 ± 0.039	2.224ThrPhe: 2.224 ± 0.034	4.68ThrGly: 4.68 ± 0.052	0.996ThrHis: 0.996 ± 0.021	3.45ThrIle: 3.45 ± 0.043	2.314ThrLys: 2.314 ± 0.034	5.788ThrLeu: 5.788 ± 0.059	1.28ThrMet: 1.28 ± 0.025	1.79ThrAsn: 1.79 ± 0.03	2.562ThrPro: 2.562 ± 0.043	1.61ThrGln: 1.61 ± 0.029	2.34ThrArg: 2.34 ± 0.035	3.331ThrSer: 3.331 ± 0.042	2.915ThrThr: 2.915 ± 0.043	4.483ThrVal: 4.483 ± 0.048	0.582ThrTrp: 0.582 ± 0.018	1.914ThrTyr: 1.914 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	5.137ValAla: 5.137 ± 0.057	0.623ValCys: 0.623 ± 0.017	3.458ValAsp: 3.458 ± 0.043	4.372ValGlu: 4.372 ± 0.052	2.906ValPhe: 2.906 ± 0.038	4.185ValGly: 4.185 ± 0.051	1.349ValHis: 1.349 ± 0.026	4.801ValIle: 4.801 ± 0.048	3.676ValLys: 3.676 ± 0.044	7.443ValLeu: 7.443 ± 0.06	1.979ValMet: 1.979 ± 0.028	2.817ValAsn: 2.817 ± 0.042	2.838ValPro: 2.838 ± 0.04	2.705ValGln: 2.705 ± 0.039	3.199ValArg: 3.199 ± 0.042	4.797ValSer: 4.797 ± 0.044	4.236ValThr: 4.236 ± 0.053	4.757ValVal: 4.757 ± 0.058	0.824ValTrp: 0.824 ± 0.021	2.392ValTyr: 2.392 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	0.801TrpAla: 0.801 ± 0.021	0.104TrpCys: 0.104 ± 0.007	0.674TrpAsp: 0.674 ± 0.018	0.787TrpGlu: 0.787 ± 0.02	0.529TrpPhe: 0.529 ± 0.016	0.882TrpGly: 0.882 ± 0.023	0.24TrpHis: 0.24 ± 0.01	0.832TrpIle: 0.832 ± 0.019	0.715TrpLys: 0.715 ± 0.021	1.426TrpLeu: 1.426 ± 0.027	0.395TrpMet: 0.395 ± 0.014	0.699TrpAsn: 0.699 ± 0.019	0.346TrpPro: 0.346 ± 0.012	0.511TrpGln: 0.511 ± 0.015	0.631TrpArg: 0.631 ± 0.016	0.934TrpSer: 0.934 ± 0.024	0.689TrpThr: 0.689 ± 0.018	0.79TrpVal: 0.79 ± 0.021	0.186TrpTrp: 0.186 ± 0.009	0.41TrpTyr: 0.41 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.753TyrAla: 2.753 ± 0.034	0.325TyrCys: 0.325 ± 0.011	1.949TyrAsp: 1.949 ± 0.033	2.344TyrGlu: 2.344 ± 0.035	1.612TyrPhe: 1.612 ± 0.029	2.755TyrGly: 2.755 ± 0.036	0.786TyrHis: 0.786 ± 0.019	2.367TyrIle: 2.367 ± 0.033	1.796TyrLys: 1.796 ± 0.026	3.642TyrLeu: 3.642 ± 0.039	0.95TyrMet: 0.95 ± 0.021	1.606TyrAsn: 1.606 ± 0.03	1.557TyrPro: 1.557 ± 0.026	1.282TyrGln: 1.282 ± 0.027	2.125TyrArg: 2.125 ± 0.031	2.5TyrSer: 2.5 ± 0.033	2.045TyrThr: 2.045 ± 0.032	2.261TyrVal: 2.261 ± 0.031	0.458TyrTrp: 0.458 ± 0.016	1.518TyrTyr: 1.518 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7234 proteins (2279734 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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