Streptococcus phage SW15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7W9X8|A0A3S7W9X8_9CAUD Uncharacterized protein OS=Streptococcus phage SW15 OX=2419635 GN=SW15_044 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNWFVNHH14 pKa = 6.82RR15 pKa = 11.84GKK17 pKa = 9.04LTYY20 pKa = 11.06SMYY23 pKa = 10.77GSRR26 pKa = 11.84NGADD30 pKa = 3.25GTADD34 pKa = 3.34CSGSISQALKK44 pKa = 10.06EE45 pKa = 4.17AGIPIQGLPSTVTLGQQLAKK65 pKa = 10.58NGFYY69 pKa = 10.18RR70 pKa = 11.84VSINQDD76 pKa = 2.14WDD78 pKa = 3.55ALTGDD83 pKa = 4.07IVLMSWGADD92 pKa = 2.98MSTSGGAGGHH102 pKa = 5.81VGVMMDD108 pKa = 4.16SINFISCDD116 pKa = 3.38YY117 pKa = 9.29STQGALGQAINTYY130 pKa = 9.03PWNDD134 pKa = 3.08YY135 pKa = 10.06YY136 pKa = 11.52AANKK140 pKa = 9.23PSYY143 pKa = 10.16IEE145 pKa = 3.55VWRR148 pKa = 11.84YY149 pKa = 10.13SDD151 pKa = 5.37SAPQTNNQANTAVAPQQKK169 pKa = 10.27AYY171 pKa = 11.2YY172 pKa = 8.17EE173 pKa = 4.27ANEE176 pKa = 3.96VKK178 pKa = 10.25YY179 pKa = 11.21VNGIWQIKK187 pKa = 9.47CDD189 pKa = 4.14YY190 pKa = 9.88LCPIGFNYY198 pKa = 8.74FQNGVPVTMVNWVDD212 pKa = 3.69KK213 pKa = 11.03DD214 pKa = 4.24GNDD217 pKa = 3.97LPDD220 pKa = 4.26GADD223 pKa = 3.29QEE225 pKa = 5.13FKK227 pKa = 11.28AGMFFSFAGDD237 pKa = 3.56EE238 pKa = 4.15NNITDD243 pKa = 4.03TGQGGYY249 pKa = 10.17YY250 pKa = 9.8GGYY253 pKa = 7.73YY254 pKa = 9.41YY255 pKa = 10.76RR256 pKa = 11.84RR257 pKa = 11.84FEE259 pKa = 4.06FGQFGTVWLSCWNKK273 pKa = 10.66DD274 pKa = 3.89DD275 pKa = 5.14LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7W9X7|A0A3S7W9X7_9CAUD Lysin OS=Streptococcus phage SW15 OX=2419635 GN=SW15_027 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.48NYY4 pKa = 10.03FEE6 pKa = 4.3KK7 pKa = 11.05LGVKK11 pKa = 8.49VLKK14 pKa = 10.07TMAQSAVGVIGASTLISQVDD34 pKa = 3.06WRR36 pKa = 11.84TQRR39 pKa = 11.84KK40 pKa = 7.53YY41 pKa = 11.2

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

10458

41

1517

237.7

26.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.841 ± 1.402

0.478 ± 0.112

6.263 ± 0.585

6.435 ± 0.731

4.15 ± 0.157

6.952 ± 0.546

1.224 ± 0.213

7.076 ± 0.406

7.793 ± 0.644

7.85 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.393

5.785 ± 0.465

2.811 ± 0.268

3.873 ± 0.287

4.255 ± 0.424

6.713 ± 0.749

6.378 ± 0.322

6.588 ± 0.306

1.195 ± 0.188

3.882 ± 0.523
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski