Streptococcus satellite phage Javan375

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZN56|A0A4D5ZN56_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan375 OX=2558666 GN=JavanS375_0015 PE=4 SV=1
MM1 pKa = 7.37AVDD4 pKa = 3.52AVEE7 pKa = 4.07VLTAIMNDD15 pKa = 3.64PTISPYY21 pKa = 10.94ARR23 pKa = 11.84QQSARR28 pKa = 11.84TILEE32 pKa = 4.01FAYY35 pKa = 9.67KK36 pKa = 10.12AYY38 pKa = 9.89EE39 pKa = 3.87NEE41 pKa = 4.25AIIEE45 pKa = 4.08KK46 pKa = 10.61LEE48 pKa = 3.87EE49 pKa = 4.43LEE51 pKa = 4.35TIISIEE57 pKa = 4.17DD58 pKa = 3.17KK59 pKa = 11.5GNN61 pKa = 3.21

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZLN6|A0A4D5ZLN6_9VIRU Glycerate kinase OS=Streptococcus satellite phage Javan375 OX=2558666 GN=JavanS375_0008 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 9.39ITEE5 pKa = 4.35HH6 pKa = 6.35KK7 pKa = 10.28KK8 pKa = 10.06KK9 pKa = 10.66DD10 pKa = 3.22GTTVYY15 pKa = 10.32RR16 pKa = 11.84ANIYY20 pKa = 10.55LGIDD24 pKa = 3.0RR25 pKa = 11.84VTGKK29 pKa = 9.94DD30 pKa = 3.1VKK32 pKa = 10.28TSITARR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.75EE42 pKa = 3.99VKK44 pKa = 9.99QKK46 pKa = 11.04AKK48 pKa = 9.96EE49 pKa = 3.98AEE51 pKa = 3.56IDD53 pKa = 4.39FIQNGSTRR61 pKa = 11.84FKK63 pKa = 10.21EE64 pKa = 4.27ATIKK68 pKa = 9.7TYY70 pKa = 10.93KK71 pKa = 10.24EE72 pKa = 3.71LAEE75 pKa = 4.5LWWEE79 pKa = 4.4SYY81 pKa = 9.73KK82 pKa = 9.95HH83 pKa = 4.54TVKK86 pKa = 10.77PNTQGNMSKK95 pKa = 10.0ILNKK99 pKa = 10.32HH100 pKa = 5.34ILPLFGAYY108 pKa = 9.57KK109 pKa = 10.2LDD111 pKa = 3.65KK112 pKa = 10.12LTTPLIQNIINKK124 pKa = 9.41LAGKK128 pKa = 7.87TNNGEE133 pKa = 3.72QGAYY137 pKa = 9.46LHH139 pKa = 6.15YY140 pKa = 10.8EE141 pKa = 4.35KK142 pKa = 10.34IHH144 pKa = 5.37TVNKK148 pKa = 10.31RR149 pKa = 11.84ILQYY153 pKa = 10.72GVVMKK158 pKa = 10.36AIPFNPARR166 pKa = 11.84EE167 pKa = 4.24VILPRR172 pKa = 11.84NTQKK176 pKa = 10.88EE177 pKa = 3.98KK178 pKa = 9.9RR179 pKa = 11.84QKK181 pKa = 9.99IKK183 pKa = 10.87HH184 pKa = 6.05FDD186 pKa = 3.75NIEE189 pKa = 3.89LKK191 pKa = 10.12TFLDD195 pKa = 3.88YY196 pKa = 11.45LDD198 pKa = 5.25NLDD201 pKa = 3.62SDD203 pKa = 4.38RR204 pKa = 11.84YY205 pKa = 9.86RR206 pKa = 11.84YY207 pKa = 9.53FYY209 pKa = 11.12EE210 pKa = 3.65KK211 pKa = 10.07TLYY214 pKa = 10.78NFLLATGCRR223 pKa = 11.84INEE226 pKa = 3.96ALALEE231 pKa = 4.46WSDD234 pKa = 4.1IDD236 pKa = 3.97LDD238 pKa = 3.84NAIVHH243 pKa = 4.96VTKK246 pKa = 10.11TLNRR250 pKa = 11.84MQEE253 pKa = 4.14VNSPKK258 pKa = 10.4SKK260 pKa = 10.46SSYY263 pKa = 10.15RR264 pKa = 11.84DD265 pKa = 2.72IDD267 pKa = 3.53IDD269 pKa = 3.89PKK271 pKa = 10.69TVTILKK277 pKa = 9.55QYY279 pKa = 10.47KK280 pKa = 9.52RR281 pKa = 11.84RR282 pKa = 11.84QTQEE286 pKa = 2.86AWKK289 pKa = 10.02IGQTEE294 pKa = 4.12KK295 pKa = 11.13VVFSDD300 pKa = 6.3FIHH303 pKa = 7.07EE304 pKa = 4.25YY305 pKa = 9.92PNNSTLFTRR314 pKa = 11.84LKK316 pKa = 9.31THH318 pKa = 6.73FKK320 pKa = 9.77RR321 pKa = 11.84AKK323 pKa = 8.02VPNIGFHH330 pKa = 5.98GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.74ASLLLNAGIPYY347 pKa = 10.01KK348 pKa = 10.28EE349 pKa = 3.98LQHH352 pKa = 6.73RR353 pKa = 11.84LGHH356 pKa = 5.68STLSMTMDD364 pKa = 4.17TYY366 pKa = 11.68SHH368 pKa = 7.14LSKK371 pKa = 10.81EE372 pKa = 4.43SAKK375 pKa = 10.56KK376 pKa = 10.11AVSFYY381 pKa = 9.16EE382 pKa = 4.14TALGSLL388 pKa = 4.11

Molecular weight: 45.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

2947

39

409

155.1

17.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.26 ± 0.4

0.543 ± 0.193

5.599 ± 0.382

8.924 ± 0.611

4.411 ± 0.487

4.309 ± 0.275

1.798 ± 0.316

7.567 ± 0.617

9.332 ± 0.54

9.976 ± 0.557

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.309

5.633 ± 0.349

2.749 ± 0.334

4.242 ± 0.407

4.343 ± 0.372

6.481 ± 0.556

5.972 ± 0.518

5.361 ± 0.34

0.78 ± 0.165

4.411 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski