Agrobacterium rosae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5403 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R3TD11|A0A1R3TD11_9RHIZ Putative aliphatic sulfonates-binding protein OS=Agrobacterium rosae OX=1972867 GN=ssuA_3 PE=3 SV=1
MM1 pKa = 7.22AQSIMNALGKK11 pKa = 9.22PLYY14 pKa = 10.7YY15 pKa = 10.32SDD17 pKa = 3.55TSTGFFSATGSGPDD31 pKa = 4.28LYY33 pKa = 10.81GTTKK37 pKa = 10.43NDD39 pKa = 3.85SMWGDD44 pKa = 3.3SGVNVTMHH52 pKa = 6.9GGTGDD57 pKa = 4.24DD58 pKa = 3.2IYY60 pKa = 11.69YY61 pKa = 10.45LYY63 pKa = 10.1STINRR68 pKa = 11.84ATEE71 pKa = 3.89AADD74 pKa = 3.79QGTDD78 pKa = 3.98TINTWMSYY86 pKa = 7.36TLPDD90 pKa = 4.13NIEE93 pKa = 3.98NLIVTGDD100 pKa = 3.34GRR102 pKa = 11.84NAFGNALDD110 pKa = 4.69NIISGGSAKK119 pKa = 8.45QTIDD123 pKa = 3.04GGAGNDD129 pKa = 3.45VLTGGGGADD138 pKa = 2.86TFVFAKK144 pKa = 10.91GNGTDD149 pKa = 4.75LITDD153 pKa = 4.62FGSDD157 pKa = 3.59DD158 pKa = 4.75FIRR161 pKa = 11.84LDD163 pKa = 3.88GYY165 pKa = 11.29GVTSFDD171 pKa = 3.95HH172 pKa = 7.08LISTSTQKK180 pKa = 11.11GADD183 pKa = 2.98LWLNFDD189 pKa = 3.62NGEE192 pKa = 4.38SVVLANTTADD202 pKa = 4.54SLDD205 pKa = 3.46ADD207 pKa = 3.98QFRR210 pKa = 11.84LTLDD214 pKa = 3.27RR215 pKa = 11.84SKK217 pKa = 10.57LTSTFADD224 pKa = 3.65EE225 pKa = 4.86FNSLSLSDD233 pKa = 3.82GQSGVWEE240 pKa = 4.37PKK242 pKa = 9.24FWWAGEE248 pKa = 4.19EE249 pKa = 4.31GGSLHH254 pKa = 6.51TNSEE258 pKa = 4.3KK259 pKa = 10.16QWYY262 pKa = 8.9INPAYY267 pKa = 10.42QPTSSVNPFSVQNGVLTITAAATPDD292 pKa = 4.29AISDD296 pKa = 3.67AVNGYY301 pKa = 10.71DD302 pKa = 3.63YY303 pKa = 10.99TSGMLTTHH311 pKa = 6.61SSFSQTYY318 pKa = 9.36GYY320 pKa = 11.18FEE322 pKa = 4.56MRR324 pKa = 11.84ADD326 pKa = 3.65MPSDD330 pKa = 3.24QGAWPAFWLLPEE342 pKa = 4.83DD343 pKa = 4.56GKK345 pKa = 10.23WPPEE349 pKa = 3.66LDD351 pKa = 3.69VIEE354 pKa = 4.62MRR356 pKa = 11.84GQNPNTLILSAHH368 pKa = 5.72SNEE371 pKa = 4.51TGQQTSTINNVNVTSTEE388 pKa = 3.97GFHH391 pKa = 6.86TYY393 pKa = 10.63GLLWDD398 pKa = 4.42EE399 pKa = 5.7DD400 pKa = 4.33HH401 pKa = 6.44ITWYY405 pKa = 10.65FDD407 pKa = 3.53DD408 pKa = 4.08VAVAQIDD415 pKa = 4.38TPTDD419 pKa = 3.25MHH421 pKa = 7.9DD422 pKa = 3.4PMYY425 pKa = 10.14MIVNLAVGGIAGTPTNGLPNGSEE448 pKa = 3.74MKK450 pKa = 9.66IDD452 pKa = 4.06YY453 pKa = 10.26IHH455 pKa = 7.39AYY457 pKa = 9.71SINDD461 pKa = 3.88PAVSAMSQTTSSDD474 pKa = 3.23NLLVV478 pKa = 3.58

Molecular weight: 51.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R3U2I2|A0A1R3U2I2_9RHIZ Putative D D-dipeptide transport system permease protein DdpC OS=Agrobacterium rosae OX=1972867 GN=ddpC_8 PE=3 SV=1
MM1 pKa = 7.45LGVHH5 pKa = 6.71LGVFFNKK12 pKa = 9.25VFSAHH17 pKa = 4.98VALAARR23 pKa = 11.84RR24 pKa = 11.84ARR26 pKa = 11.84AGSVGLNSFGRR37 pKa = 11.84FAKK40 pKa = 9.93WISASIMPPPSLVSARR56 pKa = 3.78

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5403

0

5403

1650202

29

2830

305.4

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.176 ± 0.041

0.768 ± 0.009

5.777 ± 0.025

5.713 ± 0.031

3.971 ± 0.022

7.986 ± 0.032

2.03 ± 0.016

5.929 ± 0.026

4.104 ± 0.029

9.816 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.015

3.1 ± 0.018

4.687 ± 0.023

3.313 ± 0.02

6.283 ± 0.03

6.199 ± 0.026

5.614 ± 0.022

7.314 ± 0.026

1.257 ± 0.014

2.341 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski