Herbiconiux ginsengi
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4507 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3TJS6|A0A1H3TJS6_9MICO ATP-binding cassette subfamily B OS=Herbiconiux ginsengi OX=381665 GN=SAMN05216554_4275 PE=4 SV=1
MM1 pKa = 7.62 LANKK5 pKa = 10.0 SFTLIGSGVSVMLIAALTGCSASGGVPGASKK36 pKa = 10.77 GGEE39 pKa = 3.9 LRR41 pKa = 11.84 LALLGDD47 pKa = 4.14 ISTPDD52 pKa = 3.95 PDD54 pKa = 3.53 TAYY57 pKa = 10.64 DD58 pKa = 3.79 GSEE61 pKa = 3.96 LNIVNSAYY69 pKa = 10.05 EE70 pKa = 3.98 GLLGYY75 pKa = 10.45 VPGKK79 pKa = 9.5 QEE81 pKa = 3.93 PEE83 pKa = 4.06 LTGVLATEE91 pKa = 4.85 WTASANNTVFTFRR104 pKa = 11.84 LRR106 pKa = 11.84 EE107 pKa = 3.85 GVTFHH112 pKa = 7.72 DD113 pKa = 4.45 GTPFTADD120 pKa = 4.25 AIQSSFDD127 pKa = 3.13 RR128 pKa = 11.84 RR129 pKa = 11.84 NAIGEE134 pKa = 4.41 GPSYY138 pKa = 8.43 MTAGVASVVAADD150 pKa = 4.66 DD151 pKa = 4.13 FNVTITLRR159 pKa = 11.84 QPNSSFLDD167 pKa = 3.65 LLASPFGPKK176 pKa = 9.23 MISPTALKK184 pKa = 9.39 EE185 pKa = 4.07 HH186 pKa = 6.6 PVVNGSEE193 pKa = 3.96 DD194 pKa = 3.21 WFDD197 pKa = 4.53 SNDD200 pKa = 3.68 AGTGPYY206 pKa = 9.57 TYY208 pKa = 10.94 GAFAPGTSYY217 pKa = 11.2 QLNAYY222 pKa = 8.74 GGYY225 pKa = 9.23 WGDD228 pKa = 3.43 APGYY232 pKa = 9.65 DD233 pKa = 2.96 AVSFDD238 pKa = 3.83 VFSNSATIQLEE249 pKa = 4.22 LQDD252 pKa = 4.43 GGVDD256 pKa = 3.77 GLIGYY261 pKa = 10.14 ADD263 pKa = 4.19 ASTFDD268 pKa = 3.14 QLKK271 pKa = 10.51 RR272 pKa = 11.84 SDD274 pKa = 3.96 RR275 pKa = 11.84 LSAYY279 pKa = 9.32 AFPSMEE285 pKa = 4.26 TPTMFVNPQSPDD297 pKa = 3.2 LADD300 pKa = 4.26 PDD302 pKa = 3.7 VRR304 pKa = 11.84 RR305 pKa = 11.84 SFISSIDD312 pKa = 3.62 FNALADD318 pKa = 3.67 TALADD323 pKa = 3.81 TATPTDD329 pKa = 3.64 GVFPTTLLPANLNTQAITYY348 pKa = 8.88 NPNALAQLAKK358 pKa = 10.36 GQLAGSTITIAYY370 pKa = 8.0 AQAGPAAQALSDD382 pKa = 3.79 NLAAVLNGAGIAAEE396 pKa = 4.16 SVGYY400 pKa = 8.33 DD401 pKa = 3.01 TGSYY405 pKa = 9.62 YY406 pKa = 11.06 AALTEE411 pKa = 4.46 GAAAPDD417 pKa = 3.2 ITFYY421 pKa = 11.31 SGFPDD426 pKa = 3.73 TASPEE431 pKa = 3.25 AWAFVFYY438 pKa = 10.37 TPAGGLDD445 pKa = 3.73 LFGADD450 pKa = 3.53 VPGVADD456 pKa = 6.23 LIDD459 pKa = 3.47 QATVTGDD466 pKa = 3.22 DD467 pKa = 3.56 ALYY470 pKa = 10.88 GDD472 pKa = 3.85 VAQRR476 pKa = 11.84 VSEE479 pKa = 4.13 SGYY482 pKa = 7.69 WRR484 pKa = 11.84 SVATSLGLAVFQKK497 pKa = 10.83 GISGVDD503 pKa = 2.94 RR504 pKa = 11.84 AYY506 pKa = 11.24 SPVITGILDD515 pKa = 3.23 IALIEE520 pKa = 4.34 PPAA523 pKa = 5.46
Molecular weight: 54.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.592
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.948
Patrickios 1.341
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A1H3TLW1|A0A1H3TLW1_9MICO AraC-type DNA-binding protein OS=Herbiconiux ginsengi OX=381665 GN=SAMN05216554_4304 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.23 VRR4 pKa = 11.84 NSLGSMKK11 pKa = 10.21 KK12 pKa = 7.81 MQGSQVVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINHH30 pKa = 6.31 ANPRR34 pKa = 11.84 FKK36 pKa = 10.81 ARR38 pKa = 11.84 QGG40 pKa = 3.28
Molecular weight: 4.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.501
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.237
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.083
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4507
0
4507
1471252
25
2301
326.4
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.319 ± 0.05
0.479 ± 0.009
6.157 ± 0.029
5.451 ± 0.037
3.248 ± 0.022
9.043 ± 0.038
1.967 ± 0.02
4.625 ± 0.028
1.925 ± 0.023
9.999 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.657 ± 0.013
2.138 ± 0.022
5.531 ± 0.028
2.721 ± 0.021
6.872 ± 0.044
6.181 ± 0.026
6.275 ± 0.04
8.906 ± 0.038
1.46 ± 0.016
2.046 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here