Wuhan insect virus 27
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFC1|A0A1L3KFC1_9VIRU LA-virus_coat domain-containing protein OS=Wuhan insect virus 27 OX=1923731 PE=4 SV=1
MM1 pKa = 7.91 PLNFVNSLTNFSVSRR16 pKa = 11.84 KK17 pKa = 9.01 YY18 pKa = 10.91 VPSVKK23 pKa = 10.15 NGRR26 pKa = 11.84 FSVINDD32 pKa = 3.65 TVATMHH38 pKa = 7.1 AANSTYY44 pKa = 10.98 NCDD47 pKa = 3.33 LQLRR51 pKa = 11.84 TDD53 pKa = 4.37 FFYY56 pKa = 11.13 SGANTKK62 pKa = 10.16 LVEE65 pKa = 4.32 IDD67 pKa = 3.87 CHH69 pKa = 7.0 SSYY72 pKa = 11.22 YY73 pKa = 10.6 GYY75 pKa = 10.08 NRR77 pKa = 11.84 KK78 pKa = 10.14 YY79 pKa = 10.0 IDD81 pKa = 3.45 EE82 pKa = 4.46 NGAYY86 pKa = 9.85 DD87 pKa = 3.73 PSLAIEE93 pKa = 4.23 EE94 pKa = 4.4 FARR97 pKa = 11.84 VAPEE101 pKa = 3.6 KK102 pKa = 10.49 RR103 pKa = 11.84 VVKK106 pKa = 10.23 SDD108 pKa = 3.38 VMALLQSHH116 pKa = 6.1 TQRR119 pKa = 11.84 DD120 pKa = 3.49 SHH122 pKa = 5.96 EE123 pKa = 4.54 AYY125 pKa = 10.16 LFNMLISYY133 pKa = 8.82 GFALLKK139 pKa = 10.79 KK140 pKa = 9.23 MNNDD144 pKa = 3.07 KK145 pKa = 11.44 LEE147 pKa = 4.15 TLNISTPEE155 pKa = 3.9 YY156 pKa = 10.13 RR157 pKa = 11.84 DD158 pKa = 2.8 SHH160 pKa = 5.66 YY161 pKa = 10.91 QVNLADD167 pKa = 3.58 STGAITQEE175 pKa = 3.93 IEE177 pKa = 3.93 LGVPNDD183 pKa = 4.43 CEE185 pKa = 5.61 EE186 pKa = 4.01 MDD188 pKa = 3.88 QPLWDD193 pKa = 4.31 LRR195 pKa = 11.84 RR196 pKa = 11.84 EE197 pKa = 3.96 DD198 pKa = 4.42 NYY200 pKa = 10.92 FKK202 pKa = 10.78 KK203 pKa = 10.24 PYY205 pKa = 8.24 YY206 pKa = 9.94 LHH208 pKa = 6.76 YY209 pKa = 9.98 NAASRR214 pKa = 11.84 GQEE217 pKa = 3.91 VFYY220 pKa = 10.54 LSHH223 pKa = 5.8 VFGRR227 pKa = 11.84 KK228 pKa = 8.57 RR229 pKa = 11.84 RR230 pKa = 11.84 SRR232 pKa = 11.84 VNFDD236 pKa = 3.94 LDD238 pKa = 2.96 ISGIDD243 pKa = 3.74 TEE245 pKa = 5.02 QLTLDD250 pKa = 3.84 PLNGKK255 pKa = 10.12 GYY257 pKa = 10.75 LPASYY262 pKa = 10.8 DD263 pKa = 3.37 GVPWDD268 pKa = 3.94 KK269 pKa = 11.44 PEE271 pKa = 4.08 TLWMWIVDD279 pKa = 3.83 YY280 pKa = 11.54 VKK282 pKa = 10.91 LNRR285 pKa = 11.84 LEE287 pKa = 4.3 QNFAPVVEE295 pKa = 4.9 CFAAVATQPLWEE307 pKa = 4.33 SSEE310 pKa = 4.11 ACVWQLAKK318 pKa = 10.53 KK319 pKa = 9.72 VVSLSSFSPTRR330 pKa = 11.84 ARR332 pKa = 11.84 LRR334 pKa = 11.84 TNLDD338 pKa = 3.16 GEE340 pKa = 4.72 PYY342 pKa = 10.56 VVDD345 pKa = 4.04 PEE347 pKa = 4.42 AVSFLYY353 pKa = 11.08 NEE355 pKa = 4.38 VVDD358 pKa = 4.15 PASYY362 pKa = 10.74 LYY364 pKa = 9.84 TAVVANYY371 pKa = 9.33 YY372 pKa = 9.88 EE373 pKa = 5.09 LYY375 pKa = 10.94 GLYY378 pKa = 9.82 TLLDD382 pKa = 3.43 NEE384 pKa = 4.36 AANRR388 pKa = 11.84 EE389 pKa = 3.96 NWRR392 pKa = 11.84 DD393 pKa = 3.61 VYY395 pKa = 11.11 TSTIDD400 pKa = 3.34 DD401 pKa = 4.09 LGVIKK406 pKa = 9.17 THH408 pKa = 6.65 LCRR411 pKa = 11.84 AACVSVVTGLEE422 pKa = 3.69 IGTTMHH428 pKa = 6.88 EE429 pKa = 4.66 GCFTYY434 pKa = 10.96 YY435 pKa = 9.62 DD436 pKa = 3.63 TSDD439 pKa = 3.05 MSFCEE444 pKa = 4.03 EE445 pKa = 3.92 LTDD448 pKa = 4.82 CVSLDD453 pKa = 3.74 GSPATIPITTIPAPVSGSLVLGTLSGPFATVQHH486 pKa = 6.35 LKK488 pKa = 10.66 GLQKK492 pKa = 10.94 LPVHH496 pKa = 6.79 EE497 pKa = 4.75 DD498 pKa = 2.85 RR499 pKa = 11.84 KK500 pKa = 9.77 HH501 pKa = 5.87 AYY503 pKa = 9.08 NAIEE507 pKa = 4.26 VVQLANVYY515 pKa = 10.45 RR516 pKa = 11.84 LFGNDD521 pKa = 2.71 TTFQSLYY528 pKa = 7.6 GQKK531 pKa = 9.66 MINPWATAATCVTQMHH547 pKa = 6.04 STGATPVFDD556 pKa = 4.84 DD557 pKa = 5.29 GYY559 pKa = 10.9 LIVQSEE565 pKa = 4.31 PRR567 pKa = 11.84 GGRR570 pKa = 11.84 HH571 pKa = 4.78 YY572 pKa = 10.64 VIPNLRR578 pKa = 11.84 SLTARR583 pKa = 11.84 DD584 pKa = 3.52 EE585 pKa = 4.16 ATLTIRR591 pKa = 11.84 QPMLTVTDD599 pKa = 4.12 FQKK602 pKa = 10.06 RR603 pKa = 11.84 TLEE606 pKa = 3.74 ARR608 pKa = 11.84 PFVKK612 pKa = 10.19 SEE614 pKa = 4.19 KK615 pKa = 8.43 ITHH618 pKa = 6.09 KK619 pKa = 11.24 AEE621 pKa = 4.29 FKK623 pKa = 10.61 IKK625 pKa = 10.1 ATMSTLKK632 pKa = 10.37 NRR634 pKa = 11.84 FIAKK638 pKa = 8.59 PVPSMPNEE646 pKa = 3.86 SVFRR650 pKa = 11.84 FNPVTTTPRR659 pKa = 11.84 MPEE662 pKa = 3.98 GTRR665 pKa = 11.84 VTATQEE671 pKa = 3.76 ATEE674 pKa = 5.16 DD675 pKa = 3.37 IVLTTQSMDD684 pKa = 3.01 NAGAVEE690 pKa = 3.94
Molecular weight: 77.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.146
IPC2_protein 5.245
IPC_protein 5.232
Toseland 5.296
ProMoST 5.372
Dawson 5.296
Bjellqvist 5.397
Wikipedia 5.194
Rodwell 5.219
Grimsley 5.27
Solomon 5.296
Lehninger 5.258
Nozaki 5.448
DTASelect 5.614
Thurlkill 5.347
EMBOSS 5.321
Sillero 5.525
Patrickios 3.668
IPC_peptide 5.308
IPC2_peptide 5.525
IPC2.peptide.svr19 5.449
Protein with the highest isoelectric point:
>tr|A0A1L3KFC1|A0A1L3KFC1_9VIRU LA-virus_coat domain-containing protein OS=Wuhan insect virus 27 OX=1923731 PE=4 SV=1
MM1 pKa = 7.0 TVEE4 pKa = 4.1 PTEE7 pKa = 4.0 EE8 pKa = 3.81 RR9 pKa = 11.84 SVFRR13 pKa = 11.84 DD14 pKa = 3.12 YY15 pKa = 11.2 RR16 pKa = 11.84 FRR18 pKa = 11.84 DD19 pKa = 3.53 GAVPTGIRR27 pKa = 11.84 ISGGGSLEE35 pKa = 3.93 AAPFSDD41 pKa = 3.88 ADD43 pKa = 3.65 FVLVDD48 pKa = 4.62 RR49 pKa = 11.84 IRR51 pKa = 11.84 GKK53 pKa = 10.48 FNQGPVVIDD62 pKa = 3.48 VLNTSAAGFAVPGPGYY78 pKa = 8.28 TFLYY82 pKa = 9.46 MRR84 pKa = 11.84 VNQEE88 pKa = 4.04 LVPVNPQVMALAARR102 pKa = 11.84 HH103 pKa = 5.49 FMGDD107 pKa = 3.15 FNVYY111 pKa = 10.77 YY112 pKa = 10.79 NDD114 pKa = 4.74 LSDD117 pKa = 4.06 LKK119 pKa = 10.94 NVRR122 pKa = 11.84 LVLGEE127 pKa = 4.07 SDD129 pKa = 3.27 NKK131 pKa = 10.11 IRR133 pKa = 11.84 NNATLQALPKK143 pKa = 9.15 PKK145 pKa = 9.78 ISGSHH150 pKa = 6.66 HH151 pKa = 5.27 IHH153 pKa = 5.53 FTAAEE158 pKa = 4.1 VWGVLTDD165 pKa = 3.72 QEE167 pKa = 4.67 KK168 pKa = 9.95 EE169 pKa = 3.48 RR170 pKa = 11.84 AMQYY174 pKa = 10.39 RR175 pKa = 11.84 RR176 pKa = 11.84 LGCDD180 pKa = 2.74 LTTSFVAGVMIWLVSLNPEE199 pKa = 4.09 LYY201 pKa = 10.09 EE202 pKa = 4.31 LVAASDD208 pKa = 4.73 LLDD211 pKa = 3.75 SMTVKK216 pKa = 10.48 EE217 pKa = 4.28 FAKK220 pKa = 10.07 KK221 pKa = 10.2 AKK223 pKa = 9.77 RR224 pKa = 11.84 LSVQAKK230 pKa = 8.74 SLQNVVTQDD239 pKa = 3.35 LRR241 pKa = 11.84 VLFEE245 pKa = 5.42 ADD247 pKa = 3.35 VLVNRR252 pKa = 11.84 VTGEE256 pKa = 3.91 VDD258 pKa = 2.57 WAGEE262 pKa = 4.02 KK263 pKa = 8.28 EE264 pKa = 4.46 HH265 pKa = 6.53 RR266 pKa = 11.84 TKK268 pKa = 11.16 CNLAQISPQRR278 pKa = 11.84 VKK280 pKa = 10.9 EE281 pKa = 3.9 VATRR285 pKa = 11.84 LFNEE289 pKa = 3.83 NNALGEE295 pKa = 4.05 RR296 pKa = 11.84 PRR298 pKa = 11.84 RR299 pKa = 11.84 FEE301 pKa = 4.58 WKK303 pKa = 10.37 KK304 pKa = 9.18 FWNNRR309 pKa = 11.84 WQWSAAGSIHH319 pKa = 5.63 SQYY322 pKa = 10.95 SEE324 pKa = 4.22 DD325 pKa = 3.48 MQFVSKK331 pKa = 10.51 QRR333 pKa = 11.84 EE334 pKa = 4.19 LKK336 pKa = 10.65 NKK338 pKa = 9.79 FIALITMPDD347 pKa = 3.23 MPIEE351 pKa = 4.12 YY352 pKa = 9.81 FLEE355 pKa = 4.3 RR356 pKa = 11.84 KK357 pKa = 9.67 PEE359 pKa = 3.56 IRR361 pKa = 11.84 AWSSIKK367 pKa = 10.66 YY368 pKa = 8.94 EE369 pKa = 3.9 WGKK372 pKa = 10.34 LRR374 pKa = 11.84 AIYY377 pKa = 8.57 GTDD380 pKa = 2.92 LTSYY384 pKa = 9.95 VLSHH388 pKa = 6.41 FAFYY392 pKa = 10.82 NCEE395 pKa = 4.07 DD396 pKa = 3.69 VLPAHH401 pKa = 6.48 FPVGKK406 pKa = 9.7 KK407 pKa = 9.89 ARR409 pKa = 11.84 PSYY412 pKa = 9.02 VRR414 pKa = 11.84 SRR416 pKa = 11.84 IRR418 pKa = 11.84 SILSGSIPFCIDD430 pKa = 3.16 FEE432 pKa = 4.87 DD433 pKa = 5.38 FNSQHH438 pKa = 6.54 SNEE441 pKa = 4.15 SMIAVIEE448 pKa = 4.64 AYY450 pKa = 10.68 LEE452 pKa = 4.4 VYY454 pKa = 10.11 QEE456 pKa = 4.34 SFSKK460 pKa = 9.31 EE461 pKa = 3.65 QIMALQWTIQSIKK474 pKa = 7.88 EE475 pKa = 3.93 THH477 pKa = 6.64 VIDD480 pKa = 4.2 NMGTKK485 pKa = 9.28 TEE487 pKa = 4.13 YY488 pKa = 8.36 QTKK491 pKa = 7.78 GTLMSGWRR499 pKa = 11.84 LTTFVNSVLNYY510 pKa = 9.1 VYY512 pKa = 10.05 TRR514 pKa = 11.84 EE515 pKa = 4.42 LIGEE519 pKa = 4.45 SKK521 pKa = 9.17 TVTRR525 pKa = 11.84 SVHH528 pKa = 6.05 NGDD531 pKa = 4.2 DD532 pKa = 3.64 VLVGITNLAVARR544 pKa = 11.84 DD545 pKa = 3.88 VVRR548 pKa = 11.84 NARR551 pKa = 11.84 KK552 pKa = 10.08 YY553 pKa = 9.62 NVRR556 pKa = 11.84 LQTVKK561 pKa = 11.12 SNLGSIAEE569 pKa = 4.1 FLRR572 pKa = 11.84 VDD574 pKa = 3.88 HH575 pKa = 7.11 ARR577 pKa = 11.84 GEE579 pKa = 4.21 YY580 pKa = 9.74 GQYY583 pKa = 8.87 LTRR586 pKa = 11.84 NIATLVHH593 pKa = 6.39 SRR595 pKa = 11.84 IEE597 pKa = 4.11 SQKK600 pKa = 10.61 AVSLIDD606 pKa = 4.85 LIEE609 pKa = 4.51 AMEE612 pKa = 4.31 DD613 pKa = 3.03 RR614 pKa = 11.84 FGEE617 pKa = 4.26 FFTRR621 pKa = 11.84 GGSMDD626 pKa = 5.52 LITRR630 pKa = 11.84 LRR632 pKa = 11.84 DD633 pKa = 2.97 KK634 pKa = 10.72 YY635 pKa = 8.38 YY636 pKa = 10.26 QHH638 pKa = 6.72 VAPIYY643 pKa = 9.25 NATVEE648 pKa = 3.84 IAYY651 pKa = 9.39 KK652 pKa = 10.12 IKK654 pKa = 10.17 QAHH657 pKa = 5.86 KK658 pKa = 9.66 VVGGASRR665 pKa = 11.84 LADD668 pKa = 3.42 ASINYY673 pKa = 9.36 IIKK676 pKa = 9.25 QAKK679 pKa = 6.92 TVSEE683 pKa = 4.2 VEE685 pKa = 4.3 LPKK688 pKa = 10.57 KK689 pKa = 10.8 LPGLDD694 pKa = 3.53 SYY696 pKa = 11.92 ADD698 pKa = 3.67 EE699 pKa = 5.75 LIASLGLSRR708 pKa = 11.84 HH709 pKa = 5.18 RR710 pKa = 11.84 KK711 pKa = 8.05 KK712 pKa = 10.75 VRR714 pKa = 11.84 EE715 pKa = 3.95 RR716 pKa = 11.84 VQQATLNAVQLVRR729 pKa = 11.84 KK730 pKa = 9.58 AISVVSNVDD739 pKa = 3.27 MKK741 pKa = 10.79 RR742 pKa = 11.84 SRR744 pKa = 11.84 VLRR747 pKa = 11.84 ALYY750 pKa = 10.11 KK751 pKa = 10.5 SYY753 pKa = 11.59 ADD755 pKa = 3.77 VADD758 pKa = 3.74 SPMFGKK764 pKa = 10.55 AKK766 pKa = 8.33 MTGFVIDD773 pKa = 3.67 VLANSTKK780 pKa = 10.54 LRR782 pKa = 11.84 TLSMKK787 pKa = 10.43 LRR789 pKa = 11.84 GSQDD793 pKa = 3.6 PIALLSVITT802 pKa = 4.34
Molecular weight: 90.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.776
IPC2_protein 8.77
IPC_protein 8.682
Toseland 9.341
ProMoST 9.165
Dawson 9.648
Bjellqvist 9.37
Wikipedia 9.838
Rodwell 9.867
Grimsley 9.736
Solomon 9.677
Lehninger 9.619
Nozaki 9.399
DTASelect 9.355
Thurlkill 9.472
EMBOSS 9.794
Sillero 9.575
Patrickios 4.889
IPC_peptide 9.663
IPC2_peptide 7.907
IPC2.peptide.svr19 7.751
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1492
690
802
746.0
84.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.708 ± 0.207
1.005 ± 0.385
5.429 ± 0.335
6.032 ± 0.036
4.088 ± 0.02
5.027 ± 0.254
2.078 ± 0.063
4.759 ± 0.647
5.295 ± 0.618
8.847 ± 0.004
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.413 ± 0.061
4.96 ± 0.263
4.088 ± 0.737
3.552 ± 0.238
6.233 ± 0.758
7.306 ± 0.228
6.702 ± 1.112
8.78 ± 0.339
1.273 ± 0.074
4.424 ± 0.518
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here