Changjiang tombus-like virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KG69|A0A1L3KG69_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 14 OX=1922807 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 4.68RR3 pKa = 11.84RR4 pKa = 11.84MPTPGIVYY12 pKa = 7.51THH14 pKa = 6.54NNSLDD19 pKa = 3.41NVLRR23 pKa = 11.84GIGEE27 pKa = 4.21RR28 pKa = 11.84LKK30 pKa = 10.37MVSDD34 pKa = 3.85GRR36 pKa = 11.84GGFCFPPRR44 pKa = 11.84PKK46 pKa = 10.65VFDD49 pKa = 3.78LEE51 pKa = 4.56EE52 pKa = 3.96YY53 pKa = 10.37RR54 pKa = 11.84SRR56 pKa = 11.84LLRR59 pKa = 11.84KK60 pKa = 8.68MPKK63 pKa = 9.29FDD65 pKa = 4.72APITLDD71 pKa = 3.87EE72 pKa = 4.58FVQLYY77 pKa = 10.1DD78 pKa = 3.32GPKK81 pKa = 8.26RR82 pKa = 11.84KK83 pKa = 9.53RR84 pKa = 11.84YY85 pKa = 9.05EE86 pKa = 4.0SAVEE90 pKa = 3.66QLVRR94 pKa = 11.84DD95 pKa = 4.2GLQPIDD101 pKa = 3.81GDD103 pKa = 3.26IALFIKK109 pKa = 10.34DD110 pKa = 4.15EE111 pKa = 5.04KK112 pKa = 9.69ICSWSKK118 pKa = 10.5VDD120 pKa = 3.71PAPRR124 pKa = 11.84LISPRR129 pKa = 11.84SPKK132 pKa = 10.47YY133 pKa = 9.55CVQLGRR139 pKa = 11.84YY140 pKa = 7.8IKK142 pKa = 10.23PIEE145 pKa = 4.26HH146 pKa = 7.24LLYY149 pKa = 10.69KK150 pKa = 10.52AVARR154 pKa = 11.84VWGEE158 pKa = 3.6TTIAKK163 pKa = 9.78GLNFNEE169 pKa = 4.14RR170 pKa = 11.84GVLIQQKK177 pKa = 8.65WEE179 pKa = 4.15SFNDD183 pKa = 3.85PVAVGLDD190 pKa = 3.17ASRR193 pKa = 11.84FDD195 pKa = 3.44QHH197 pKa = 7.91VSEE200 pKa = 4.87LALMWEE206 pKa = 4.13HH207 pKa = 5.91SVYY210 pKa = 9.29MRR212 pKa = 11.84CYY214 pKa = 9.85PRR216 pKa = 11.84EE217 pKa = 3.94EE218 pKa = 4.44CDD220 pKa = 3.23GKK222 pKa = 10.57LARR225 pKa = 11.84LLEE228 pKa = 4.35RR229 pKa = 11.84QLVNRR234 pKa = 11.84GRR236 pKa = 11.84CYY238 pKa = 10.77VEE240 pKa = 3.6NHH242 pKa = 5.06LVEE245 pKa = 4.34YY246 pKa = 7.64EE247 pKa = 4.05HH248 pKa = 7.23RR249 pKa = 11.84GGRR252 pKa = 11.84MSGDD256 pKa = 3.3MNTALGNCLIMTGLVWEE273 pKa = 5.01HH274 pKa = 5.81ARR276 pKa = 11.84QLGVTVKK283 pKa = 10.74LINDD287 pKa = 3.59GDD289 pKa = 4.07DD290 pKa = 3.08CVVFMEE296 pKa = 4.4RR297 pKa = 11.84ADD299 pKa = 3.65LARR302 pKa = 11.84YY303 pKa = 9.74LDD305 pKa = 4.45GLEE308 pKa = 3.53EE309 pKa = 4.07WFRR312 pKa = 11.84ARR314 pKa = 11.84GFTMKK319 pKa = 10.2VEE321 pKa = 4.28KK322 pKa = 9.89PAFEE326 pKa = 4.46LEE328 pKa = 4.12QIEE331 pKa = 4.71FCQCRR336 pKa = 11.84PVWNGEE342 pKa = 3.9QYY344 pKa = 10.35TMCRR348 pKa = 11.84NVFKK352 pKa = 10.97ALFTDD357 pKa = 5.38GVHH360 pKa = 6.26VGRR363 pKa = 11.84TLSEE367 pKa = 3.79IQHH370 pKa = 5.87IRR372 pKa = 11.84SATSKK377 pKa = 10.58CGKK380 pKa = 9.1VWSKK384 pKa = 10.76GLPIFGEE391 pKa = 4.6FYY393 pKa = 11.05EE394 pKa = 4.59FLSCEE399 pKa = 4.02APRR402 pKa = 11.84GKK404 pKa = 10.9SNFYY408 pKa = 10.22GDD410 pKa = 3.92YY411 pKa = 9.75RR412 pKa = 11.84HH413 pKa = 7.03SGTVWQAKK421 pKa = 8.83GCVSGTTHH429 pKa = 5.91ITDD432 pKa = 3.25EE433 pKa = 4.34ARR435 pKa = 11.84ASFHH439 pKa = 6.4RR440 pKa = 11.84AFGITGSEE448 pKa = 3.93QVLVEE453 pKa = 5.0DD454 pKa = 5.81FYY456 pKa = 12.1KK457 pKa = 10.79DD458 pKa = 3.86LPKK461 pKa = 8.72TTYY464 pKa = 7.68DD465 pKa = 3.62TPQDD469 pKa = 3.39ALVYY473 pKa = 9.98NPQISPDD480 pKa = 3.74SYY482 pKa = 10.59PLFVSEE488 pKa = 4.64SLCEE492 pKa = 3.83IVFNKK497 pKa = 10.3

Molecular weight:
57.06 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KG69|A0A1L3KG69_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 14 OX=1922807 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.49TKK5 pKa = 10.21VKK7 pKa = 10.1KK8 pKa = 10.66VKK10 pKa = 10.52ASSQYY15 pKa = 7.57QRR17 pKa = 11.84KK18 pKa = 6.01MTGMYY23 pKa = 9.59KK24 pKa = 10.01QLEE27 pKa = 4.25LGKK30 pKa = 10.01LDD32 pKa = 4.84KK33 pKa = 10.79EE34 pKa = 4.39ALQAAAMYY42 pKa = 10.26ADD44 pKa = 4.08PCGADD49 pKa = 4.07LVPSVYY55 pKa = 10.43PGDD58 pKa = 3.22RR59 pKa = 11.84GFINRR64 pKa = 11.84FTNYY68 pKa = 8.37NTVGNGATEE77 pKa = 4.17TCAIMIWRR85 pKa = 11.84PGTQGLYY92 pKa = 9.49QAGAVTSATTAAVTYY107 pKa = 7.95GTFFPGLPFLNTNASKK123 pKa = 9.41TRR125 pKa = 11.84CAAFCATVRR134 pKa = 11.84PVSAPNTATGTIYY147 pKa = 10.63FGIVNARR154 pKa = 11.84AVRR157 pKa = 11.84SGATLSPNDD166 pKa = 4.09YY167 pKa = 10.6ISLCSEE173 pKa = 4.31SVSCSQALMAPLEE186 pKa = 4.43VRR188 pKa = 11.84WSPGDD193 pKa = 3.19LDD195 pKa = 3.79ARR197 pKa = 11.84YY198 pKa = 10.41SEE200 pKa = 4.45VNNPNVAADD209 pKa = 3.77DD210 pKa = 3.49SDD212 pKa = 4.8YY213 pKa = 11.75NVLIVVAIGIPANTGMQFRR232 pKa = 11.84ATAVMEE238 pKa = 4.25WAPNANIGITNDD250 pKa = 3.14ATSVKK255 pKa = 10.12PSACDD260 pKa = 3.34KK261 pKa = 10.51EE262 pKa = 5.15CVINYY267 pKa = 8.09LKK269 pKa = 10.91KK270 pKa = 10.04KK271 pKa = 10.57DD272 pKa = 4.03RR273 pKa = 11.84DD274 pKa = 3.32WWWNLGKK281 pKa = 8.92KK282 pKa = 7.03TLNVGKK288 pKa = 10.25SVVQGYY294 pKa = 6.8YY295 pKa = 9.47TGGAIGAMGSLLKK308 pKa = 10.33YY309 pKa = 10.74AKK311 pKa = 10.41

Molecular weight:
33.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

808

311

497

404.0

45.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.045 ± 2.748

2.847 ± 0.156

5.446 ± 0.539

5.569 ± 1.71

4.084 ± 0.862

7.921 ± 0.251

1.485 ± 0.847

4.332 ± 0.086

5.817 ± 0.35

7.673 ± 0.892

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.168

4.455 ± 1.127

4.95 ± 0.073

3.218 ± 0.185

6.312 ± 1.4

5.817 ± 0.35

5.693 ± 1.338

7.55 ± 0.096

1.856 ± 0.042

4.332 ± 0.28

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski