Changjiang tombus-like virus 14
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KG69|A0A1L3KG69_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 14 OX=1922807 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 4.68 RR3 pKa = 11.84 RR4 pKa = 11.84 MPTPGIVYY12 pKa = 7.51 THH14 pKa = 6.54 NNSLDD19 pKa = 3.41 NVLRR23 pKa = 11.84 GIGEE27 pKa = 4.21 RR28 pKa = 11.84 LKK30 pKa = 10.37 MVSDD34 pKa = 3.85 GRR36 pKa = 11.84 GGFCFPPRR44 pKa = 11.84 PKK46 pKa = 10.65 VFDD49 pKa = 3.78 LEE51 pKa = 4.56 EE52 pKa = 3.96 YY53 pKa = 10.37 RR54 pKa = 11.84 SRR56 pKa = 11.84 LLRR59 pKa = 11.84 KK60 pKa = 8.68 MPKK63 pKa = 9.29 FDD65 pKa = 4.72 APITLDD71 pKa = 3.87 EE72 pKa = 4.58 FVQLYY77 pKa = 10.1 DD78 pKa = 3.32 GPKK81 pKa = 8.26 RR82 pKa = 11.84 KK83 pKa = 9.53 RR84 pKa = 11.84 YY85 pKa = 9.05 EE86 pKa = 4.0 SAVEE90 pKa = 3.66 QLVRR94 pKa = 11.84 DD95 pKa = 4.2 GLQPIDD101 pKa = 3.81 GDD103 pKa = 3.26 IALFIKK109 pKa = 10.34 DD110 pKa = 4.15 EE111 pKa = 5.04 KK112 pKa = 9.69 ICSWSKK118 pKa = 10.5 VDD120 pKa = 3.71 PAPRR124 pKa = 11.84 LISPRR129 pKa = 11.84 SPKK132 pKa = 10.47 YY133 pKa = 9.55 CVQLGRR139 pKa = 11.84 YY140 pKa = 7.8 IKK142 pKa = 10.23 PIEE145 pKa = 4.26 HH146 pKa = 7.24 LLYY149 pKa = 10.69 KK150 pKa = 10.52 AVARR154 pKa = 11.84 VWGEE158 pKa = 3.6 TTIAKK163 pKa = 9.78 GLNFNEE169 pKa = 4.14 RR170 pKa = 11.84 GVLIQQKK177 pKa = 8.65 WEE179 pKa = 4.15 SFNDD183 pKa = 3.85 PVAVGLDD190 pKa = 3.17 ASRR193 pKa = 11.84 FDD195 pKa = 3.44 QHH197 pKa = 7.91 VSEE200 pKa = 4.87 LALMWEE206 pKa = 4.13 HH207 pKa = 5.91 SVYY210 pKa = 9.29 MRR212 pKa = 11.84 CYY214 pKa = 9.85 PRR216 pKa = 11.84 EE217 pKa = 3.94 EE218 pKa = 4.44 CDD220 pKa = 3.23 GKK222 pKa = 10.57 LARR225 pKa = 11.84 LLEE228 pKa = 4.35 RR229 pKa = 11.84 QLVNRR234 pKa = 11.84 GRR236 pKa = 11.84 CYY238 pKa = 10.77 VEE240 pKa = 3.6 NHH242 pKa = 5.06 LVEE245 pKa = 4.34 YY246 pKa = 7.64 EE247 pKa = 4.05 HH248 pKa = 7.23 RR249 pKa = 11.84 GGRR252 pKa = 11.84 MSGDD256 pKa = 3.3 MNTALGNCLIMTGLVWEE273 pKa = 5.01 HH274 pKa = 5.81 ARR276 pKa = 11.84 QLGVTVKK283 pKa = 10.74 LINDD287 pKa = 3.59 GDD289 pKa = 4.07 DD290 pKa = 3.08 CVVFMEE296 pKa = 4.4 RR297 pKa = 11.84 ADD299 pKa = 3.65 LARR302 pKa = 11.84 YY303 pKa = 9.74 LDD305 pKa = 4.45 GLEE308 pKa = 3.53 EE309 pKa = 4.07 WFRR312 pKa = 11.84 ARR314 pKa = 11.84 GFTMKK319 pKa = 10.2 VEE321 pKa = 4.28 KK322 pKa = 9.89 PAFEE326 pKa = 4.46 LEE328 pKa = 4.12 QIEE331 pKa = 4.71 FCQCRR336 pKa = 11.84 PVWNGEE342 pKa = 3.9 QYY344 pKa = 10.35 TMCRR348 pKa = 11.84 NVFKK352 pKa = 10.97 ALFTDD357 pKa = 5.38 GVHH360 pKa = 6.26 VGRR363 pKa = 11.84 TLSEE367 pKa = 3.79 IQHH370 pKa = 5.87 IRR372 pKa = 11.84 SATSKK377 pKa = 10.58 CGKK380 pKa = 9.1 VWSKK384 pKa = 10.76 GLPIFGEE391 pKa = 4.6 FYY393 pKa = 11.05 EE394 pKa = 4.59 FLSCEE399 pKa = 4.02 APRR402 pKa = 11.84 GKK404 pKa = 10.9 SNFYY408 pKa = 10.22 GDD410 pKa = 3.92 YY411 pKa = 9.75 RR412 pKa = 11.84 HH413 pKa = 7.03 SGTVWQAKK421 pKa = 8.83 GCVSGTTHH429 pKa = 5.91 ITDD432 pKa = 3.25 EE433 pKa = 4.34 ARR435 pKa = 11.84 ASFHH439 pKa = 6.4 RR440 pKa = 11.84 AFGITGSEE448 pKa = 3.93 QVLVEE453 pKa = 5.0 DD454 pKa = 5.81 FYY456 pKa = 12.1 KK457 pKa = 10.79 DD458 pKa = 3.86 LPKK461 pKa = 8.72 TTYY464 pKa = 7.68 DD465 pKa = 3.62 TPQDD469 pKa = 3.39 ALVYY473 pKa = 9.98 NPQISPDD480 pKa = 3.74 SYY482 pKa = 10.59 PLFVSEE488 pKa = 4.64 SLCEE492 pKa = 3.83 IVFNKK497 pKa = 10.3
Molecular weight: 57.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.304
IPC2_protein 6.376
IPC_protein 6.427
Toseland 6.478
ProMoST 6.912
Dawson 6.854
Bjellqvist 6.868
Wikipedia 6.839
Rodwell 6.854
Grimsley 6.561
Solomon 6.868
Lehninger 6.868
Nozaki 7.176
DTASelect 7.234
Thurlkill 7.263
EMBOSS 7.249
Sillero 7.293
Patrickios 4.151
IPC_peptide 6.883
IPC2_peptide 6.971
IPC2.peptide.svr19 6.825
Protein with the highest isoelectric point:
>tr|A0A1L3KG69|A0A1L3KG69_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 14 OX=1922807 PE=4 SV=1
MM1 pKa = 7.8 AKK3 pKa = 9.49 TKK5 pKa = 10.21 VKK7 pKa = 10.1 KK8 pKa = 10.66 VKK10 pKa = 10.52 ASSQYY15 pKa = 7.57 QRR17 pKa = 11.84 KK18 pKa = 6.01 MTGMYY23 pKa = 9.59 KK24 pKa = 10.01 QLEE27 pKa = 4.25 LGKK30 pKa = 10.01 LDD32 pKa = 4.84 KK33 pKa = 10.79 EE34 pKa = 4.39 ALQAAAMYY42 pKa = 10.26 ADD44 pKa = 4.08 PCGADD49 pKa = 4.07 LVPSVYY55 pKa = 10.43 PGDD58 pKa = 3.22 RR59 pKa = 11.84 GFINRR64 pKa = 11.84 FTNYY68 pKa = 8.37 NTVGNGATEE77 pKa = 4.17 TCAIMIWRR85 pKa = 11.84 PGTQGLYY92 pKa = 9.49 QAGAVTSATTAAVTYY107 pKa = 7.95 GTFFPGLPFLNTNASKK123 pKa = 9.41 TRR125 pKa = 11.84 CAAFCATVRR134 pKa = 11.84 PVSAPNTATGTIYY147 pKa = 10.63 FGIVNARR154 pKa = 11.84 AVRR157 pKa = 11.84 SGATLSPNDD166 pKa = 4.09 YY167 pKa = 10.6 ISLCSEE173 pKa = 4.31 SVSCSQALMAPLEE186 pKa = 4.43 VRR188 pKa = 11.84 WSPGDD193 pKa = 3.19 LDD195 pKa = 3.79 ARR197 pKa = 11.84 YY198 pKa = 10.41 SEE200 pKa = 4.45 VNNPNVAADD209 pKa = 3.77 DD210 pKa = 3.49 SDD212 pKa = 4.8 YY213 pKa = 11.75 NVLIVVAIGIPANTGMQFRR232 pKa = 11.84 ATAVMEE238 pKa = 4.25 WAPNANIGITNDD250 pKa = 3.14 ATSVKK255 pKa = 10.12 PSACDD260 pKa = 3.34 KK261 pKa = 10.51 EE262 pKa = 5.15 CVINYY267 pKa = 8.09 LKK269 pKa = 10.91 KK270 pKa = 10.04 KK271 pKa = 10.57 DD272 pKa = 4.03 RR273 pKa = 11.84 DD274 pKa = 3.32 WWWNLGKK281 pKa = 8.92 KK282 pKa = 7.03 TLNVGKK288 pKa = 10.25 SVVQGYY294 pKa = 6.8 YY295 pKa = 9.47 TGGAIGAMGSLLKK308 pKa = 10.33 YY309 pKa = 10.74 AKK311 pKa = 10.41
Molecular weight: 33.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.518
IPC2_protein 8.419
IPC_protein 8.302
Toseland 8.624
ProMoST 8.873
Dawson 9.107
Bjellqvist 9.151
Wikipedia 9.224
Rodwell 9.209
Grimsley 8.916
Solomon 9.194
Lehninger 9.165
Nozaki 9.268
DTASelect 8.975
Thurlkill 9.048
EMBOSS 9.253
Sillero 9.282
Patrickios 4.088
IPC_peptide 9.18
IPC2_peptide 8.009
IPC2.peptide.svr19 7.822
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
808
311
497
404.0
45.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.045 ± 2.748
2.847 ± 0.156
5.446 ± 0.539
5.569 ± 1.71
4.084 ± 0.862
7.921 ± 0.251
1.485 ± 0.847
4.332 ± 0.086
5.817 ± 0.35
7.673 ± 0.892
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.168
4.455 ± 1.127
4.95 ± 0.073
3.218 ± 0.185
6.312 ± 1.4
5.817 ± 0.35
5.693 ± 1.338
7.55 ± 0.096
1.856 ± 0.042
4.332 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here