Amino acid dipepetide frequency for Pectinobacterium phage vB_PcaM_CBB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.342AlaAla: 4.342 ± 0.305
0.456AlaCys: 0.456 ± 0.065
3.664AlaAsp: 3.664 ± 0.196
4.166AlaGlu: 4.166 ± 0.264
2.557AlaPhe: 2.557 ± 0.165
3.794AlaGly: 3.794 ± 0.285
0.986AlaHis: 0.986 ± 0.094
4.017AlaIle: 4.017 ± 0.173
4.249AlaLys: 4.249 ± 0.227
4.566AlaLeu: 4.566 ± 0.229
1.85AlaMet: 1.85 ± 0.121
3.264AlaAsn: 3.264 ± 0.193
1.832AlaPro: 1.832 ± 0.139
2.232AlaGln: 2.232 ± 0.19
2.473AlaArg: 2.473 ± 0.166
3.487AlaSer: 3.487 ± 0.221
3.059AlaThr: 3.059 ± 0.302
4.045AlaVal: 4.045 ± 0.216
0.651AlaTrp: 0.651 ± 0.074
2.269AlaTyr: 2.269 ± 0.155
0.0AlaXaa: 0.0 ± 0.0
Cys
0.511CysAla: 0.511 ± 0.089
0.195CysCys: 0.195 ± 0.045
0.642CysAsp: 0.642 ± 0.087
0.911CysGlu: 0.911 ± 0.083
0.428CysPhe: 0.428 ± 0.069
0.79CysGly: 0.79 ± 0.097
0.205CysHis: 0.205 ± 0.046
0.735CysIle: 0.735 ± 0.084
0.781CysLys: 0.781 ± 0.109
0.735CysLeu: 0.735 ± 0.096
0.325CysMet: 0.325 ± 0.048
0.614CysAsn: 0.614 ± 0.072
0.418CysPro: 0.418 ± 0.069
0.446CysGln: 0.446 ± 0.071
0.353CysArg: 0.353 ± 0.058
0.921CysSer: 0.921 ± 0.089
0.986CysThr: 0.986 ± 0.094
0.716CysVal: 0.716 ± 0.084
0.158CysTrp: 0.158 ± 0.034
0.428CysTyr: 0.428 ± 0.067
0.0CysXaa: 0.0 ± 0.0
Asp
4.222AspAla: 4.222 ± 0.19
0.753AspCys: 0.753 ± 0.094
4.352AspAsp: 4.352 ± 0.268
5.644AspGlu: 5.644 ± 0.295
3.227AspPhe: 3.227 ± 0.184
4.37AspGly: 4.37 ± 0.212
1.069AspHis: 1.069 ± 0.094
4.668AspIle: 4.668 ± 0.234
3.757AspLys: 3.757 ± 0.222
5.142AspLeu: 5.142 ± 0.281
1.888AspMet: 1.888 ± 0.145
3.552AspAsn: 3.552 ± 0.219
2.222AspPro: 2.222 ± 0.129
1.618AspGln: 1.618 ± 0.138
2.399AspArg: 2.399 ± 0.156
4.166AspSer: 4.166 ± 0.168
3.85AspThr: 3.85 ± 0.185
4.705AspVal: 4.705 ± 0.213
0.921AspTrp: 0.921 ± 0.101
3.654AspTyr: 3.654 ± 0.191
0.0AspXaa: 0.0 ± 0.0
Glu
4.138GluAla: 4.138 ± 0.249
0.967GluCys: 0.967 ± 0.109
4.779GluAsp: 4.779 ± 0.267
6.211GluGlu: 6.211 ± 0.31
3.915GluPhe: 3.915 ± 0.165
3.199GluGly: 3.199 ± 0.184
1.72GluHis: 1.72 ± 0.124
4.993GluIle: 4.993 ± 0.246
4.342GluLys: 4.342 ± 0.288
6.667GluLeu: 6.667 ± 0.311
2.566GluMet: 2.566 ± 0.168
3.803GluAsn: 3.803 ± 0.201
2.139GluPro: 2.139 ± 0.152
3.134GluGln: 3.134 ± 0.182
3.087GluArg: 3.087 ± 0.194
3.896GluSer: 3.896 ± 0.212
3.626GluThr: 3.626 ± 0.197
5.049GluVal: 5.049 ± 0.206
0.967GluTrp: 0.967 ± 0.107
3.896GluTyr: 3.896 ± 0.221
0.0GluXaa: 0.0 ± 0.0
Phe
2.334PheAla: 2.334 ± 0.134
0.66PheCys: 0.66 ± 0.085
3.543PheAsp: 3.543 ± 0.177
3.58PheGlu: 3.58 ± 0.161
1.925PhePhe: 1.925 ± 0.156
3.059PheGly: 3.059 ± 0.186
0.837PheHis: 0.837 ± 0.1
3.143PheIle: 3.143 ± 0.159
3.366PheLys: 3.366 ± 0.182
2.994PheLeu: 2.994 ± 0.182
1.209PheMet: 1.209 ± 0.114
3.124PheAsn: 3.124 ± 0.19
1.348PhePro: 1.348 ± 0.126
1.246PheGln: 1.246 ± 0.114
1.46PheArg: 1.46 ± 0.099
2.966PheSer: 2.966 ± 0.183
2.687PheThr: 2.687 ± 0.176
3.394PheVal: 3.394 ± 0.162
0.79PheTrp: 0.79 ± 0.084
1.85PheTyr: 1.85 ± 0.123
0.0PheXaa: 0.0 ± 0.0
Gly
2.892GlyAla: 2.892 ± 0.221
0.669GlyCys: 0.669 ± 0.09
3.636GlyAsp: 3.636 ± 0.207
3.822GlyGlu: 3.822 ± 0.199
2.594GlyPhe: 2.594 ± 0.174
3.031GlyGly: 3.031 ± 0.284
1.125GlyHis: 1.125 ± 0.118
4.212GlyIle: 4.212 ± 0.189
4.119GlyLys: 4.119 ± 0.239
4.147GlyLeu: 4.147 ± 0.213
1.376GlyMet: 1.376 ± 0.109
3.682GlyAsn: 3.682 ± 0.325
0.623GlyPro: 0.623 ± 0.073
2.027GlyGln: 2.027 ± 0.134
2.26GlyArg: 2.26 ± 0.153
4.621GlySer: 4.621 ± 0.214
5.412GlyThr: 5.412 ± 0.492
3.803GlyVal: 3.803 ± 0.186
0.688GlyTrp: 0.688 ± 0.083
3.217GlyTyr: 3.217 ± 0.201
0.0GlyXaa: 0.0 ± 0.0
His
0.976HisAla: 0.976 ± 0.096
0.26HisCys: 0.26 ± 0.05
1.367HisAsp: 1.367 ± 0.121
1.478HisGlu: 1.478 ± 0.116
1.069HisPhe: 1.069 ± 0.115
1.265HisGly: 1.265 ± 0.118
0.437HisHis: 0.437 ± 0.075
1.274HisIle: 1.274 ± 0.109
1.255HisLys: 1.255 ± 0.129
1.469HisLeu: 1.469 ± 0.118
0.632HisMet: 0.632 ± 0.084
1.181HisAsn: 1.181 ± 0.121
0.837HisPro: 0.837 ± 0.081
0.586HisGln: 0.586 ± 0.08
0.707HisArg: 0.707 ± 0.086
1.395HisSer: 1.395 ± 0.119
1.097HisThr: 1.097 ± 0.105
1.125HisVal: 1.125 ± 0.101
0.279HisTrp: 0.279 ± 0.06
0.921HisTyr: 0.921 ± 0.103
0.0HisXaa: 0.0 ± 0.0
Ile
4.417IleAla: 4.417 ± 0.232
0.772IleCys: 0.772 ± 0.094
4.705IleAsp: 4.705 ± 0.229
5.198IleGlu: 5.198 ± 0.279
2.52IlePhe: 2.52 ± 0.169
3.571IleGly: 3.571 ± 0.182
1.497IleHis: 1.497 ± 0.13
4.696IleIle: 4.696 ± 0.231
4.742IleLys: 4.742 ± 0.235
4.975IleLeu: 4.975 ± 0.225
1.813IleMet: 1.813 ± 0.158
4.538IleAsn: 4.538 ± 0.243
2.418IlePro: 2.418 ± 0.152
2.752IleGln: 2.752 ± 0.151
3.227IleArg: 3.227 ± 0.169
4.696IleSer: 4.696 ± 0.267
4.24IleThr: 4.24 ± 0.227
4.324IleVal: 4.324 ± 0.177
0.725IleTrp: 0.725 ± 0.088
2.65IleTyr: 2.65 ± 0.15
0.0IleXaa: 0.0 ± 0.0
Lys
3.599LysAla: 3.599 ± 0.225
0.614LysCys: 0.614 ± 0.083
3.998LysAsp: 3.998 ± 0.198
5.077LysGlu: 5.077 ± 0.288
2.976LysPhe: 2.976 ± 0.193
2.985LysGly: 2.985 ± 0.168
1.488LysHis: 1.488 ± 0.131
4.5LysIle: 4.5 ± 0.239
4.249LysLys: 4.249 ± 0.262
5.421LysLeu: 5.421 ± 0.262
2.315LysMet: 2.315 ± 0.144
4.036LysAsn: 4.036 ± 0.202
2.148LysPro: 2.148 ± 0.156
2.464LysGln: 2.464 ± 0.154
3.05LysArg: 3.05 ± 0.18
3.515LysSer: 3.515 ± 0.173
3.571LysThr: 3.571 ± 0.206
4.863LysVal: 4.863 ± 0.205
0.623LysTrp: 0.623 ± 0.096
3.998LysTyr: 3.998 ± 0.18
0.0LysXaa: 0.0 ± 0.0
Leu
4.77LeuAla: 4.77 ± 0.256
0.883LeuCys: 0.883 ± 0.096
5.179LeuAsp: 5.179 ± 0.209
5.458LeuGlu: 5.458 ± 0.241
3.134LeuPhe: 3.134 ± 0.184
3.915LeuGly: 3.915 ± 0.196
1.553LeuHis: 1.553 ± 0.113
4.398LeuIle: 4.398 ± 0.212
5.598LeuLys: 5.598 ± 0.274
5.588LeuLeu: 5.588 ± 0.252
2.287LeuMet: 2.287 ± 0.15
5.365LeuAsn: 5.365 ± 0.204
3.031LeuPro: 3.031 ± 0.201
2.985LeuGln: 2.985 ± 0.18
3.571LeuArg: 3.571 ± 0.192
5.653LeuSer: 5.653 ± 0.216
4.575LeuThr: 4.575 ± 0.316
4.863LeuVal: 4.863 ± 0.26
0.865LeuTrp: 0.865 ± 0.091
3.301LeuTyr: 3.301 ± 0.188
0.0LeuXaa: 0.0 ± 0.0
Met
1.878MetAla: 1.878 ± 0.124
0.307MetCys: 0.307 ± 0.057
2.046MetAsp: 2.046 ± 0.146
1.739MetGlu: 1.739 ± 0.129
1.478MetPhe: 1.478 ± 0.135
1.237MetGly: 1.237 ± 0.121
0.502MetHis: 0.502 ± 0.068
1.962MetIle: 1.962 ± 0.141
2.511MetLys: 2.511 ± 0.144
2.036MetLeu: 2.036 ± 0.131
0.707MetMet: 0.707 ± 0.078
2.12MetAsn: 2.12 ± 0.162
0.809MetPro: 0.809 ± 0.1
1.041MetGln: 1.041 ± 0.101
1.172MetArg: 1.172 ± 0.112
1.971MetSer: 1.971 ± 0.126
1.609MetThr: 1.609 ± 0.128
1.544MetVal: 1.544 ± 0.129
0.344MetTrp: 0.344 ± 0.054
1.516MetTyr: 1.516 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
3.682AsnAla: 3.682 ± 0.212
0.669AsnCys: 0.669 ± 0.102
3.747AsnAsp: 3.747 ± 0.173
4.11AsnGlu: 4.11 ± 0.244
2.622AsnPhe: 2.622 ± 0.146
4.686AsnGly: 4.686 ± 0.251
1.079AsnHis: 1.079 ± 0.082
4.389AsnIle: 4.389 ± 0.227
3.766AsnLys: 3.766 ± 0.158
4.222AsnLeu: 4.222 ± 0.195
1.664AsnMet: 1.664 ± 0.143
3.803AsnAsn: 3.803 ± 0.227
2.771AsnPro: 2.771 ± 0.203
1.813AsnGln: 1.813 ± 0.133
2.492AsnArg: 2.492 ± 0.143
4.361AsnSer: 4.361 ± 0.209
4.287AsnThr: 4.287 ± 0.289
4.054AsnVal: 4.054 ± 0.302
0.511AsnTrp: 0.511 ± 0.069
2.529AsnTyr: 2.529 ± 0.192
0.0AsnXaa: 0.0 ± 0.0
Pro
1.906ProAla: 1.906 ± 0.132
0.325ProCys: 0.325 ± 0.053
2.669ProAsp: 2.669 ± 0.176
2.994ProGlu: 2.994 ± 0.174
1.655ProPhe: 1.655 ± 0.126
1.86ProGly: 1.86 ± 0.124
0.53ProHis: 0.53 ± 0.079
2.204ProIle: 2.204 ± 0.159
1.915ProLys: 1.915 ± 0.13
2.167ProLeu: 2.167 ± 0.133
0.707ProMet: 0.707 ± 0.083
2.055ProAsn: 2.055 ± 0.123
0.772ProPro: 0.772 ± 0.086
1.134ProGln: 1.134 ± 0.114
1.144ProArg: 1.144 ± 0.102
2.25ProSer: 2.25 ± 0.174
2.036ProThr: 2.036 ± 0.149
2.446ProVal: 2.446 ± 0.134
0.288ProTrp: 0.288 ± 0.061
1.534ProTyr: 1.534 ± 0.121
0.0ProXaa: 0.0 ± 0.0
Gln
1.897GlnAla: 1.897 ± 0.155
0.381GlnCys: 0.381 ± 0.06
1.943GlnAsp: 1.943 ± 0.149
2.91GlnGlu: 2.91 ± 0.177
1.674GlnPhe: 1.674 ± 0.136
1.637GlnGly: 1.637 ± 0.144
0.707GlnHis: 0.707 ± 0.078
2.641GlnIle: 2.641 ± 0.152
2.436GlnLys: 2.436 ± 0.155
2.873GlnLeu: 2.873 ± 0.191
1.311GlnMet: 1.311 ± 0.113
2.297GlnAsn: 2.297 ± 0.174
0.967GlnPro: 0.967 ± 0.099
1.367GlnGln: 1.367 ± 0.114
1.841GlnArg: 1.841 ± 0.132
2.194GlnSer: 2.194 ± 0.152
1.73GlnThr: 1.73 ± 0.129
2.074GlnVal: 2.074 ± 0.141
0.493GlnTrp: 0.493 ± 0.06
2.446GlnTyr: 2.446 ± 0.153
0.0GlnXaa: 0.0 ± 0.0
Arg
2.101ArgAla: 2.101 ± 0.139
0.456ArgCys: 0.456 ± 0.062
2.455ArgAsp: 2.455 ± 0.16
2.92ArgGlu: 2.92 ± 0.22
2.074ArgPhe: 2.074 ± 0.144
2.325ArgGly: 2.325 ± 0.139
0.772ArgHis: 0.772 ± 0.079
2.892ArgIle: 2.892 ± 0.163
3.096ArgLys: 3.096 ± 0.217
3.208ArgLeu: 3.208 ± 0.181
1.246ArgMet: 1.246 ± 0.107
2.697ArgAsn: 2.697 ± 0.154
1.069ArgPro: 1.069 ± 0.107
1.311ArgGln: 1.311 ± 0.091
1.841ArgArg: 1.841 ± 0.148
2.501ArgSer: 2.501 ± 0.175
2.371ArgThr: 2.371 ± 0.159
2.631ArgVal: 2.631 ± 0.16
0.558ArgTrp: 0.558 ± 0.08
2.157ArgTyr: 2.157 ± 0.142
0.0ArgXaa: 0.0 ± 0.0
Ser
3.933SerAla: 3.933 ± 0.212
0.632SerCys: 0.632 ± 0.09
4.082SerAsp: 4.082 ± 0.212
4.24SerGlu: 4.24 ± 0.201
3.087SerPhe: 3.087 ± 0.149
4.686SerGly: 4.686 ± 0.321
1.06SerHis: 1.06 ± 0.099
4.733SerIle: 4.733 ± 0.219
3.301SerLys: 3.301 ± 0.196
5.021SerLeu: 5.021 ± 0.227
1.599SerMet: 1.599 ± 0.122
3.757SerAsn: 3.757 ± 0.225
1.934SerPro: 1.934 ± 0.13
2.083SerGln: 2.083 ± 0.141
2.455SerArg: 2.455 ± 0.143
4.528SerSer: 4.528 ± 0.288
6.574SerThr: 6.574 ± 0.317
4.556SerVal: 4.556 ± 0.227
0.828SerTrp: 0.828 ± 0.093
2.91SerTyr: 2.91 ± 0.187
0.0SerXaa: 0.0 ± 0.0
Thr
3.97ThrAla: 3.97 ± 0.25
0.577ThrCys: 0.577 ± 0.096
3.961ThrAsp: 3.961 ± 0.204
4.045ThrGlu: 4.045 ± 0.21
3.05ThrPhe: 3.05 ± 0.218
4.491ThrGly: 4.491 ± 0.448
1.079ThrHis: 1.079 ± 0.108
4.64ThrIle: 4.64 ± 0.208
3.673ThrLys: 3.673 ± 0.178
5.096ThrLeu: 5.096 ± 0.369
1.534ThrMet: 1.534 ± 0.131
3.58ThrAsn: 3.58 ± 0.231
2.52ThrPro: 2.52 ± 0.157
2.046ThrGln: 2.046 ± 0.192
1.99ThrArg: 1.99 ± 0.138
4.324ThrSer: 4.324 ± 0.334
4.073ThrThr: 4.073 ± 0.454
5.291ThrVal: 5.291 ± 0.467
0.902ThrTrp: 0.902 ± 0.107
2.948ThrTyr: 2.948 ± 0.187
0.0ThrXaa: 0.0 ± 0.0
Val
3.478ValAla: 3.478 ± 0.179
0.725ValCys: 0.725 ± 0.085
4.64ValAsp: 4.64 ± 0.232
4.426ValGlu: 4.426 ± 0.237
2.613ValPhe: 2.613 ± 0.15
3.338ValGly: 3.338 ± 0.214
1.534ValHis: 1.534 ± 0.13
4.156ValIle: 4.156 ± 0.195
4.277ValLys: 4.277 ± 0.193
6.881ValLeu: 6.881 ± 0.279
1.832ValMet: 1.832 ± 0.128
3.794ValAsn: 3.794 ± 0.24
3.013ValPro: 3.013 ± 0.201
3.282ValGln: 3.282 ± 0.171
2.697ValArg: 2.697 ± 0.147
4.761ValSer: 4.761 ± 0.26
4.054ValThr: 4.054 ± 0.467
4.826ValVal: 4.826 ± 0.234
0.679ValTrp: 0.679 ± 0.085
3.199ValTyr: 3.199 ± 0.204
0.0ValXaa: 0.0 ± 0.0
Trp
0.577TrpAla: 0.577 ± 0.078
0.177TrpCys: 0.177 ± 0.038
0.902TrpAsp: 0.902 ± 0.099
0.781TrpGlu: 0.781 ± 0.088
0.549TrpPhe: 0.549 ± 0.078
0.604TrpGly: 0.604 ± 0.075
0.288TrpHis: 0.288 ± 0.051
0.883TrpIle: 0.883 ± 0.091
0.781TrpLys: 0.781 ± 0.084
0.79TrpLeu: 0.79 ± 0.09
0.344TrpMet: 0.344 ± 0.048
0.865TrpAsn: 0.865 ± 0.093
0.186TrpPro: 0.186 ± 0.045
0.428TrpGln: 0.428 ± 0.07
0.456TrpArg: 0.456 ± 0.064
0.818TrpSer: 0.818 ± 0.099
0.818TrpThr: 0.818 ± 0.088
0.8TrpVal: 0.8 ± 0.099
0.149TrpTrp: 0.149 ± 0.038
0.79TrpTyr: 0.79 ± 0.087
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.334TyrAla: 2.334 ± 0.144
0.818TyrCys: 0.818 ± 0.099
3.868TyrAsp: 3.868 ± 0.187
3.394TyrGlu: 3.394 ± 0.205
2.371TyrPhe: 2.371 ± 0.145
3.115TyrGly: 3.115 ± 0.192
1.144TyrHis: 1.144 ± 0.126
3.338TyrIle: 3.338 ± 0.184
3.273TyrLys: 3.273 ± 0.183
2.976TyrLeu: 2.976 ± 0.177
1.311TyrMet: 1.311 ± 0.105
3.134TyrAsn: 3.134 ± 0.216
1.534TyrPro: 1.534 ± 0.118
1.823TyrGln: 1.823 ± 0.148
2.008TyrArg: 2.008 ± 0.129
2.976TyrSer: 2.976 ± 0.175
3.134TyrThr: 3.134 ± 0.175
3.096TyrVal: 3.096 ± 0.216
0.567TyrTrp: 0.567 ± 0.076
2.38TyrTyr: 2.38 ± 0.154
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 555 proteins (107545 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski