Actinomadura craniellae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6713 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A365H9A6|A0A365H9A6_9ACTN Serine/threonine-protein phosphatase OS=Actinomadura craniellae OX=2231787 GN=DPM19_14150 PE=4 SV=1
MM1 pKa = 7.3ALEE4 pKa = 4.1VSGEE8 pKa = 3.99RR9 pKa = 11.84RR10 pKa = 11.84SGDD13 pKa = 3.05GTADD17 pKa = 3.45LVAYY21 pKa = 10.21LEE23 pKa = 4.03QDD25 pKa = 3.76DD26 pKa = 4.23PDD28 pKa = 4.55GYY30 pKa = 9.26PVHH33 pKa = 6.49RR34 pKa = 11.84VVVAVCAACSGTTFGLLADD53 pKa = 4.48DD54 pKa = 4.71AEE56 pKa = 4.67SVVRR60 pKa = 11.84RR61 pKa = 11.84TCAGCGEE68 pKa = 4.41SALMLDD74 pKa = 5.52SEE76 pKa = 5.65DD77 pKa = 3.86YY78 pKa = 10.62WDD80 pKa = 4.53DD81 pKa = 3.64ADD83 pKa = 4.65PEE85 pKa = 4.48VIEE88 pKa = 4.8CEE90 pKa = 4.19CGGEE94 pKa = 4.07EE95 pKa = 4.53FEE97 pKa = 4.62VAVGFTMRR105 pKa = 11.84TGADD109 pKa = 3.21EE110 pKa = 4.1TDD112 pKa = 3.17EE113 pKa = 4.15VRR115 pKa = 11.84WVSVGVRR122 pKa = 11.84CVRR125 pKa = 11.84DD126 pKa = 3.44GRR128 pKa = 11.84LGYY131 pKa = 10.24CADD134 pKa = 3.26WKK136 pKa = 10.5IDD138 pKa = 3.68YY139 pKa = 10.67SPSSDD144 pKa = 3.67LLDD147 pKa = 3.52RR148 pKa = 11.84VV149 pKa = 3.73

Molecular weight: 16.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A365H6R7|A0A365H6R7_9ACTN Uncharacterized protein OS=Actinomadura craniellae OX=2231787 GN=DPM19_13825 PE=4 SV=1
MM1 pKa = 7.33TGAITMRR8 pKa = 11.84DD9 pKa = 3.51RR10 pKa = 11.84VHH12 pKa = 6.93SSAGRR17 pKa = 11.84LIRR20 pKa = 11.84GWITSTRR27 pKa = 11.84VAALVITRR35 pKa = 11.84RR36 pKa = 11.84SSARR40 pKa = 11.84ARR42 pKa = 11.84ASQPVTSRR50 pKa = 11.84SRR52 pKa = 11.84STAFASPSLSSTNVPAGVATGRR74 pKa = 11.84FTPHH78 pKa = 5.18STRR81 pKa = 11.84PAAPVPAPAHH91 pKa = 5.69RR92 pKa = 11.84AWPGSPRR99 pKa = 11.84RR100 pKa = 3.8

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6713

0

6713

2180549

27

9536

324.8

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.584 ± 0.049

0.795 ± 0.008

5.952 ± 0.022

5.789 ± 0.027

2.737 ± 0.015

9.532 ± 0.03

2.259 ± 0.016

3.434 ± 0.021

1.755 ± 0.023

10.592 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.013

1.669 ± 0.015

6.46 ± 0.033

2.617 ± 0.02

8.837 ± 0.034

4.479 ± 0.019

5.746 ± 0.024

8.443 ± 0.028

1.529 ± 0.013

2.027 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski