Amino acid dipepetide frequency for Thermus phage YS40_Isch

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.656AlaAla: 1.656 ± 0.216
0.256AlaCys: 0.256 ± 0.065
1.972AlaAsp: 1.972 ± 0.198
2.819AlaGlu: 2.819 ± 0.288
2.76AlaPhe: 2.76 ± 0.215
1.912AlaGly: 1.912 ± 0.23
0.473AlaHis: 0.473 ± 0.083
3.707AlaIle: 3.707 ± 0.248
4.416AlaLys: 4.416 ± 0.283
5.323AlaLeu: 5.323 ± 0.398
0.808AlaMet: 0.808 ± 0.133
2.918AlaAsn: 2.918 ± 0.208
1.341AlaPro: 1.341 ± 0.154
1.755AlaGln: 1.755 ± 0.192
1.893AlaArg: 1.893 ± 0.245
3.667AlaSer: 3.667 ± 0.326
2.642AlaThr: 2.642 ± 0.26
2.701AlaVal: 2.701 ± 0.219
0.414AlaTrp: 0.414 ± 0.098
2.07AlaTyr: 2.07 ± 0.192
0.0AlaXaa: 0.0 ± 0.0
Cys
0.256CysAla: 0.256 ± 0.077
0.02CysCys: 0.02 ± 0.02
0.237CysAsp: 0.237 ± 0.064
0.355CysGlu: 0.355 ± 0.08
0.355CysPhe: 0.355 ± 0.081
0.375CysGly: 0.375 ± 0.109
0.079CysHis: 0.079 ± 0.036
0.256CysIle: 0.256 ± 0.09
0.355CysLys: 0.355 ± 0.086
0.394CysLeu: 0.394 ± 0.104
0.039CysMet: 0.039 ± 0.032
0.315CysAsn: 0.315 ± 0.078
0.355CysPro: 0.355 ± 0.086
0.138CysGln: 0.138 ± 0.054
0.197CysArg: 0.197 ± 0.058
0.651CysSer: 0.651 ± 0.141
0.177CysThr: 0.177 ± 0.055
0.315CysVal: 0.315 ± 0.08
0.059CysTrp: 0.059 ± 0.034
0.256CysTyr: 0.256 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
2.819AspAla: 2.819 ± 0.297
0.276AspCys: 0.276 ± 0.085
3.036AspAsp: 3.036 ± 0.266
4.988AspGlu: 4.988 ± 0.283
4.673AspPhe: 4.673 ± 0.274
2.011AspGly: 2.011 ± 0.212
0.434AspHis: 0.434 ± 0.092
4.693AspIle: 4.693 ± 0.328
4.456AspLys: 4.456 ± 0.405
5.54AspLeu: 5.54 ± 0.301
0.651AspMet: 0.651 ± 0.104
2.543AspAsn: 2.543 ± 0.229
1.558AspPro: 1.558 ± 0.223
0.868AspGln: 0.868 ± 0.1
1.893AspArg: 1.893 ± 0.205
3.115AspSer: 3.115 ± 0.215
2.642AspThr: 2.642 ± 0.226
3.904AspVal: 3.904 ± 0.314
0.473AspTrp: 0.473 ± 0.098
3.411AspTyr: 3.411 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
4.14GluAla: 4.14 ± 0.393
0.217GluCys: 0.217 ± 0.058
4.929GluAsp: 4.929 ± 0.432
9.306GluGlu: 9.306 ± 0.804
4.712GluPhe: 4.712 ± 0.306
3.49GluGly: 3.49 ± 0.239
1.36GluHis: 1.36 ± 0.161
6.94GluIle: 6.94 ± 0.426
8.616GluLys: 8.616 ± 0.61
6.92GluLeu: 6.92 ± 0.438
1.321GluMet: 1.321 ± 0.169
6.152GluAsn: 6.152 ± 0.312
1.676GluPro: 1.676 ± 0.172
2.07GluGln: 2.07 ± 0.261
2.938GluArg: 2.938 ± 0.271
4.259GluSer: 4.259 ± 0.389
3.569GluThr: 3.569 ± 0.342
6.073GluVal: 6.073 ± 0.461
0.335GluTrp: 0.335 ± 0.086
3.786GluTyr: 3.786 ± 0.285
0.0GluXaa: 0.0 ± 0.0
Phe
2.918PheAla: 2.918 ± 0.225
0.552PheCys: 0.552 ± 0.094
3.667PheAsp: 3.667 ± 0.29
4.87PheGlu: 4.87 ± 0.379
4.081PhePhe: 4.081 ± 0.339
2.997PheGly: 2.997 ± 0.267
0.73PheHis: 0.73 ± 0.127
3.983PheIle: 3.983 ± 0.314
4.594PheLys: 4.594 ± 0.304
6.664PheLeu: 6.664 ± 0.44
1.144PheMet: 1.144 ± 0.147
3.805PheAsn: 3.805 ± 0.274
2.051PhePro: 2.051 ± 0.179
0.868PheGln: 0.868 ± 0.135
2.366PheArg: 2.366 ± 0.236
6.605PheSer: 6.605 ± 0.437
2.78PheThr: 2.78 ± 0.264
5.146PheVal: 5.146 ± 0.343
0.453PheTrp: 0.453 ± 0.103
4.633PheTyr: 4.633 ± 0.36
0.0PheXaa: 0.0 ± 0.0
Gly
2.228GlyAla: 2.228 ± 0.28
0.256GlyCys: 0.256 ± 0.078
2.07GlyAsp: 2.07 ± 0.213
3.194GlyGlu: 3.194 ± 0.233
2.583GlyPhe: 2.583 ± 0.198
3.135GlyGly: 3.135 ± 0.374
0.513GlyHis: 0.513 ± 0.113
3.805GlyIle: 3.805 ± 0.26
4.357GlyLys: 4.357 ± 0.277
3.746GlyLeu: 3.746 ± 0.301
0.927GlyMet: 0.927 ± 0.135
3.45GlyAsn: 3.45 ± 0.322
0.907GlyPro: 0.907 ± 0.123
1.262GlyGln: 1.262 ± 0.192
1.893GlyArg: 1.893 ± 0.202
3.352GlySer: 3.352 ± 0.381
2.721GlyThr: 2.721 ± 0.289
2.997GlyVal: 2.997 ± 0.241
0.453GlyTrp: 0.453 ± 0.099
2.287GlyTyr: 2.287 ± 0.207
0.0GlyXaa: 0.0 ± 0.0
His
0.473HisAla: 0.473 ± 0.083
0.079HisCys: 0.079 ± 0.035
0.513HisAsp: 0.513 ± 0.091
0.848HisGlu: 0.848 ± 0.126
0.69HisPhe: 0.69 ± 0.096
0.493HisGly: 0.493 ± 0.096
0.217HisHis: 0.217 ± 0.067
1.36HisIle: 1.36 ± 0.164
1.163HisLys: 1.163 ± 0.176
1.479HisLeu: 1.479 ± 0.169
0.099HisMet: 0.099 ± 0.04
0.453HisAsn: 0.453 ± 0.085
0.67HisPro: 0.67 ± 0.113
0.237HisGln: 0.237 ± 0.072
0.375HisArg: 0.375 ± 0.094
0.769HisSer: 0.769 ± 0.135
0.532HisThr: 0.532 ± 0.101
0.67HisVal: 0.67 ± 0.1
0.059HisTrp: 0.059 ± 0.028
0.513HisTyr: 0.513 ± 0.095
0.0HisXaa: 0.0 ± 0.0
Ile
3.825IleAla: 3.825 ± 0.272
0.394IleCys: 0.394 ± 0.089
4.574IleAsp: 4.574 ± 0.274
6.447IleGlu: 6.447 ± 0.352
4.909IlePhe: 4.909 ± 0.337
3.135IleGly: 3.135 ± 0.241
1.006IleHis: 1.006 ± 0.132
4.693IleIle: 4.693 ± 0.331
7.098IleLys: 7.098 ± 0.435
7.078IleLeu: 7.078 ± 0.374
0.927IleMet: 0.927 ± 0.154
5.185IleAsn: 5.185 ± 0.479
3.036IlePro: 3.036 ± 0.203
1.952IleGln: 1.952 ± 0.185
3.056IleArg: 3.056 ± 0.256
6.861IleSer: 6.861 ± 0.397
3.884IleThr: 3.884 ± 0.256
4.555IleVal: 4.555 ± 0.306
0.335IleTrp: 0.335 ± 0.084
4.85IleTyr: 4.85 ± 0.373
0.0IleXaa: 0.0 ± 0.0
Lys
3.845LysAla: 3.845 ± 0.28
0.217LysCys: 0.217 ± 0.072
5.264LysAsp: 5.264 ± 0.329
9.72LysGlu: 9.72 ± 0.858
5.205LysPhe: 5.205 ± 0.433
3.036LysGly: 3.036 ± 0.228
1.124LysHis: 1.124 ± 0.181
7.689LysIle: 7.689 ± 0.543
7.965LysLys: 7.965 ± 0.656
7.571LysLeu: 7.571 ± 0.433
1.42LysMet: 1.42 ± 0.184
6.408LysAsn: 6.408 ± 0.393
2.11LysPro: 2.11 ± 0.177
2.287LysGln: 2.287 ± 0.229
4.101LysArg: 4.101 ± 0.306
4.752LysSer: 4.752 ± 0.347
4.555LysThr: 4.555 ± 0.366
5.698LysVal: 5.698 ± 0.323
0.394LysTrp: 0.394 ± 0.1
4.278LysTyr: 4.278 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
4.456LeuAla: 4.456 ± 0.354
0.552LeuCys: 0.552 ± 0.11
6.112LeuAsp: 6.112 ± 0.393
8.222LeuGlu: 8.222 ± 0.471
5.146LeuPhe: 5.146 ± 0.342
4.969LeuGly: 4.969 ± 0.321
1.045LeuHis: 1.045 ± 0.137
6.881LeuIle: 6.881 ± 0.329
9.05LeuLys: 9.05 ± 0.438
8.577LeuLeu: 8.577 ± 0.43
1.518LeuMet: 1.518 ± 0.181
6.704LeuAsn: 6.704 ± 0.425
3.746LeuPro: 3.746 ± 0.225
2.681LeuGln: 2.681 ± 0.251
3.963LeuArg: 3.963 ± 0.282
8.596LeuSer: 8.596 ± 0.467
4.476LeuThr: 4.476 ± 0.318
5.659LeuVal: 5.659 ± 0.293
0.513LeuTrp: 0.513 ± 0.1
5.087LeuTyr: 5.087 ± 0.346
0.0LeuXaa: 0.0 ± 0.0
Met
0.572MetAla: 0.572 ± 0.091
0.079MetCys: 0.079 ± 0.04
0.513MetAsp: 0.513 ± 0.102
0.828MetGlu: 0.828 ± 0.132
1.144MetPhe: 1.144 ± 0.168
0.67MetGly: 0.67 ± 0.112
0.296MetHis: 0.296 ± 0.077
1.045MetIle: 1.045 ± 0.154
1.715MetLys: 1.715 ± 0.168
1.459MetLeu: 1.459 ± 0.179
0.414MetMet: 0.414 ± 0.097
0.69MetAsn: 0.69 ± 0.133
0.591MetPro: 0.591 ± 0.108
0.513MetGln: 0.513 ± 0.099
0.67MetArg: 0.67 ± 0.123
1.242MetSer: 1.242 ± 0.153
0.907MetThr: 0.907 ± 0.134
0.71MetVal: 0.71 ± 0.132
0.039MetTrp: 0.039 ± 0.031
0.611MetTyr: 0.611 ± 0.115
0.0MetXaa: 0.0 ± 0.0
Asn
2.977AsnAla: 2.977 ± 0.199
0.355AsnCys: 0.355 ± 0.096
3.273AsnAsp: 3.273 ± 0.249
4.693AsnGlu: 4.693 ± 0.331
4.673AsnPhe: 4.673 ± 0.271
2.839AsnGly: 2.839 ± 0.313
0.71AsnHis: 0.71 ± 0.112
5.461AsnIle: 5.461 ± 0.381
5.185AsnLys: 5.185 ± 0.358
7.571AsnLeu: 7.571 ± 0.429
0.532AsnMet: 0.532 ± 0.107
4.062AsnAsn: 4.062 ± 0.355
2.701AsnPro: 2.701 ± 0.282
1.715AsnGln: 1.715 ± 0.234
2.267AsnArg: 2.267 ± 0.248
4.416AsnSer: 4.416 ± 0.417
3.864AsnThr: 3.864 ± 0.385
3.45AsnVal: 3.45 ± 0.282
0.394AsnTrp: 0.394 ± 0.098
3.707AsnTyr: 3.707 ± 0.325
0.0AsnXaa: 0.0 ± 0.0
Pro
1.104ProAla: 1.104 ± 0.149
0.099ProCys: 0.099 ± 0.044
1.696ProAsp: 1.696 ± 0.187
2.405ProGlu: 2.405 ± 0.271
1.991ProPhe: 1.991 ± 0.213
1.163ProGly: 1.163 ± 0.18
0.296ProHis: 0.296 ± 0.081
2.859ProIle: 2.859 ± 0.246
2.741ProLys: 2.741 ± 0.28
2.957ProLeu: 2.957 ± 0.265
0.473ProMet: 0.473 ± 0.092
2.189ProAsn: 2.189 ± 0.247
1.282ProPro: 1.282 ± 0.211
1.36ProGln: 1.36 ± 0.191
1.183ProArg: 1.183 ± 0.156
2.938ProSer: 2.938 ± 0.248
1.735ProThr: 1.735 ± 0.22
2.169ProVal: 2.169 ± 0.229
0.256ProTrp: 0.256 ± 0.064
2.031ProTyr: 2.031 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
1.36GlnAla: 1.36 ± 0.157
0.059GlnCys: 0.059 ± 0.036
1.262GlnAsp: 1.262 ± 0.152
2.484GlnGlu: 2.484 ± 0.267
1.282GlnPhe: 1.282 ± 0.151
1.4GlnGly: 1.4 ± 0.153
0.118GlnHis: 0.118 ± 0.052
2.149GlnIle: 2.149 ± 0.227
2.681GlnLys: 2.681 ± 0.264
2.287GlnLeu: 2.287 ± 0.259
0.513GlnMet: 0.513 ± 0.091
2.386GlnAsn: 2.386 ± 0.231
0.591GlnPro: 0.591 ± 0.102
1.222GlnGln: 1.222 ± 0.233
1.025GlnArg: 1.025 ± 0.148
1.498GlnSer: 1.498 ± 0.181
1.439GlnThr: 1.439 ± 0.175
1.538GlnVal: 1.538 ± 0.195
0.237GlnTrp: 0.237 ± 0.065
1.242GlnTyr: 1.242 ± 0.151
0.0GlnXaa: 0.0 ± 0.0
Arg
1.538ArgAla: 1.538 ± 0.201
0.177ArgCys: 0.177 ± 0.059
1.735ArgAsp: 1.735 ± 0.187
3.45ArgGlu: 3.45 ± 0.267
2.662ArgPhe: 2.662 ± 0.224
1.912ArgGly: 1.912 ± 0.22
0.493ArgHis: 0.493 ± 0.081
3.51ArgIle: 3.51 ± 0.288
3.569ArgLys: 3.569 ± 0.344
3.963ArgLeu: 3.963 ± 0.287
0.552ArgMet: 0.552 ± 0.108
2.622ArgAsn: 2.622 ± 0.292
1.065ArgPro: 1.065 ± 0.135
1.006ArgGln: 1.006 ± 0.15
1.912ArgArg: 1.912 ± 0.221
2.189ArgSer: 2.189 ± 0.246
1.715ArgThr: 1.715 ± 0.188
2.307ArgVal: 2.307 ± 0.187
0.158ArgTrp: 0.158 ± 0.058
1.873ArgTyr: 1.873 ± 0.237
0.0ArgXaa: 0.0 ± 0.0
Ser
3.588SerAla: 3.588 ± 0.293
0.611SerCys: 0.611 ± 0.125
3.332SerAsp: 3.332 ± 0.312
5.757SerGlu: 5.757 ± 0.499
5.816SerPhe: 5.816 ± 0.35
4.042SerGly: 4.042 ± 0.438
0.73SerHis: 0.73 ± 0.117
5.856SerIle: 5.856 ± 0.445
5.166SerLys: 5.166 ± 0.353
8.38SerLeu: 8.38 ± 0.475
1.144SerMet: 1.144 ± 0.166
4.219SerAsn: 4.219 ± 0.313
2.603SerPro: 2.603 ± 0.225
2.189SerGln: 2.189 ± 0.212
2.484SerArg: 2.484 ± 0.272
6.723SerSer: 6.723 ± 0.58
3.687SerThr: 3.687 ± 0.275
4.673SerVal: 4.673 ± 0.334
0.513SerTrp: 0.513 ± 0.102
3.943SerTyr: 3.943 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
1.834ThrAla: 1.834 ± 0.181
0.237ThrCys: 0.237 ± 0.082
2.248ThrAsp: 2.248 ± 0.233
2.898ThrGlu: 2.898 ± 0.278
3.707ThrPhe: 3.707 ± 0.313
2.287ThrGly: 2.287 ± 0.272
0.591ThrHis: 0.591 ± 0.102
4.436ThrIle: 4.436 ± 0.278
3.805ThrLys: 3.805 ± 0.3
5.757ThrLeu: 5.757 ± 0.331
0.611ThrMet: 0.611 ± 0.123
3.549ThrAsn: 3.549 ± 0.304
2.327ThrPro: 2.327 ± 0.27
1.558ThrGln: 1.558 ± 0.175
1.518ThrArg: 1.518 ± 0.175
4.298ThrSer: 4.298 ± 0.33
3.529ThrThr: 3.529 ± 0.345
2.346ThrVal: 2.346 ± 0.28
0.335ThrTrp: 0.335 ± 0.091
2.208ThrTyr: 2.208 ± 0.242
0.0ThrXaa: 0.0 ± 0.0
Val
3.312ValAla: 3.312 ± 0.271
0.375ValCys: 0.375 ± 0.081
4.239ValAsp: 4.239 ± 0.238
5.501ValGlu: 5.501 ± 0.368
4.535ValPhe: 4.535 ± 0.323
3.194ValGly: 3.194 ± 0.281
0.749ValHis: 0.749 ± 0.099
3.904ValIle: 3.904 ± 0.285
5.363ValLys: 5.363 ± 0.343
5.915ValLeu: 5.915 ± 0.309
0.67ValMet: 0.67 ± 0.155
3.845ValAsn: 3.845 ± 0.302
2.366ValPro: 2.366 ± 0.219
1.479ValGln: 1.479 ± 0.158
2.031ValArg: 2.031 ± 0.188
4.811ValSer: 4.811 ± 0.314
2.011ValThr: 2.011 ± 0.237
4.377ValVal: 4.377 ± 0.413
0.513ValTrp: 0.513 ± 0.105
3.845ValTyr: 3.845 ± 0.3
0.0ValXaa: 0.0 ± 0.0
Trp
0.296TrpAla: 0.296 ± 0.098
0.02TrpCys: 0.02 ± 0.019
0.355TrpAsp: 0.355 ± 0.097
0.591TrpGlu: 0.591 ± 0.128
0.473TrpPhe: 0.473 ± 0.108
0.434TrpGly: 0.434 ± 0.092
0.118TrpHis: 0.118 ± 0.043
0.473TrpIle: 0.473 ± 0.081
0.453TrpLys: 0.453 ± 0.099
0.414TrpLeu: 0.414 ± 0.085
0.177TrpMet: 0.177 ± 0.065
0.434TrpAsn: 0.434 ± 0.09
0.177TrpPro: 0.177 ± 0.061
0.177TrpGln: 0.177 ± 0.061
0.276TrpArg: 0.276 ± 0.058
0.375TrpSer: 0.375 ± 0.069
0.375TrpThr: 0.375 ± 0.088
0.394TrpVal: 0.394 ± 0.079
0.039TrpTrp: 0.039 ± 0.024
0.335TrpTyr: 0.335 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.307TyrAla: 2.307 ± 0.192
0.355TyrCys: 0.355 ± 0.094
2.957TyrAsp: 2.957 ± 0.235
3.431TyrGlu: 3.431 ± 0.236
3.51TyrPhe: 3.51 ± 0.286
2.642TyrGly: 2.642 ± 0.246
0.611TyrHis: 0.611 ± 0.105
4.101TyrIle: 4.101 ± 0.33
4.89TyrLys: 4.89 ± 0.347
5.954TyrLeu: 5.954 ± 0.359
0.749TyrMet: 0.749 ± 0.129
2.918TyrAsn: 2.918 ± 0.18
1.774TyrPro: 1.774 ± 0.192
1.439TyrGln: 1.439 ± 0.173
2.386TyrArg: 2.386 ± 0.205
4.338TyrSer: 4.338 ± 0.265
2.839TyrThr: 2.839 ± 0.272
3.352TyrVal: 3.352 ± 0.246
0.414TyrTrp: 0.414 ± 0.093
2.8TyrTyr: 2.8 ± 0.236
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 175 proteins (50720 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski