Lysobacter lycopersici
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2491 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516V8K4|A0A516V8K4_9GAMM Uncharacterized protein OS=Lysobacter lycopersici OX=1324796 GN=FNZ56_12545 PE=4 SV=1
MM1 pKa = 6.76 KK2 pKa = 10.34 QCMLWGDD9 pKa = 4.43 LSSDD13 pKa = 3.61 RR14 pKa = 11.84 ASEE17 pKa = 4.13 QYY19 pKa = 10.32 PEE21 pKa = 4.59 EE22 pKa = 4.36 PVCTGCIKK30 pKa = 10.88 DD31 pKa = 3.59 EE32 pKa = 4.15 EE33 pKa = 4.4 ARR35 pKa = 11.84 GEE37 pKa = 3.95 DD38 pKa = 3.49 SRR40 pKa = 11.84 IVSVGDD46 pKa = 3.5 LVTDD50 pKa = 4.35 PEE52 pKa = 4.63 AVCALCDD59 pKa = 4.98 CGFDD63 pKa = 4.29 DD64 pKa = 5.5
Molecular weight: 6.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.554
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A516V4X4|A0A516V4X4_9GAMM DUF4097 domain-containing protein OS=Lysobacter lycopersici OX=1324796 GN=FNZ56_06645 PE=4 SV=1
MM1 pKa = 7.5 AMKK4 pKa = 10.35 KK5 pKa = 9.75 AAKK8 pKa = 10.03 KK9 pKa = 8.63 PAKK12 pKa = 9.75 KK13 pKa = 9.82 AAKK16 pKa = 9.96 KK17 pKa = 10.11 KK18 pKa = 8.72 VAKK21 pKa = 10.34 KK22 pKa = 8.51 KK23 pKa = 7.08 TARR26 pKa = 11.84 KK27 pKa = 9.01 AVKK30 pKa = 9.91 KK31 pKa = 9.95 AAKK34 pKa = 8.47 KK35 pKa = 7.0 TAKK38 pKa = 10.3 KK39 pKa = 10.1 AVKK42 pKa = 9.71 KK43 pKa = 9.74 AAKK46 pKa = 9.74 RR47 pKa = 11.84 KK48 pKa = 7.59 PAKK51 pKa = 9.97 KK52 pKa = 9.65 AAKK55 pKa = 9.68 KK56 pKa = 9.13 VAKK59 pKa = 9.61 KK60 pKa = 10.34 RR61 pKa = 11.84 KK62 pKa = 7.3 PAKK65 pKa = 9.9 KK66 pKa = 9.54 AAKK69 pKa = 9.42 KK70 pKa = 9.14 AAKK73 pKa = 9.79 RR74 pKa = 11.84 KK75 pKa = 7.69 PAKK78 pKa = 9.97 KK79 pKa = 9.61 AAKK82 pKa = 9.42 KK83 pKa = 9.14 AAKK86 pKa = 9.79 RR87 pKa = 11.84 KK88 pKa = 7.69 PAKK91 pKa = 9.97 KK92 pKa = 9.61 AAKK95 pKa = 9.42 KK96 pKa = 9.14 AAKK99 pKa = 9.79 RR100 pKa = 11.84 KK101 pKa = 7.69 PAKK104 pKa = 9.96 KK105 pKa = 9.68 AAKK108 pKa = 9.49 KK109 pKa = 9.53 KK110 pKa = 10.16 AKK112 pKa = 10.22 KK113 pKa = 9.63 SVKK116 pKa = 9.98 KK117 pKa = 10.03 SGPVAMPSVPAPMII131 pKa = 3.99
Molecular weight: 13.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 11.286
IPC_protein 12.544
Toseland 12.837
ProMoST 13.217
Dawson 12.852
Bjellqvist 12.749
Wikipedia 13.247
Rodwell 13.29
Grimsley 12.881
Solomon 13.247
Lehninger 13.173
Nozaki 12.822
DTASelect 12.749
Thurlkill 12.822
EMBOSS 13.305
Sillero 12.822
Patrickios 12.998
IPC_peptide 13.261
IPC2_peptide 12.193
IPC2.peptide.svr19 8.88
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2491
0
2491
793208
33
2407
318.4
34.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.33 ± 0.067
0.888 ± 0.013
6.25 ± 0.043
5.39 ± 0.053
3.533 ± 0.033
8.605 ± 0.045
2.23 ± 0.024
4.16 ± 0.034
3.15 ± 0.043
10.503 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.025
2.562 ± 0.033
5.378 ± 0.036
3.417 ± 0.03
7.614 ± 0.066
5.073 ± 0.037
4.647 ± 0.043
7.245 ± 0.041
1.536 ± 0.025
2.272 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here