Gordonia phage Mcklovin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8DCU5|A0A5P8DCU5_9CAUD Glycosyltransferase OS=Gordonia phage Mcklovin OX=2652881 GN=31 PE=4 SV=1
MM1 pKa = 7.23PTITYY6 pKa = 9.09TLDD9 pKa = 3.76DD10 pKa = 4.35GFGTPDD16 pKa = 4.26AEE18 pKa = 5.33IDD20 pKa = 3.78LWWQPVSPHH29 pKa = 6.43GEE31 pKa = 3.85SGALVVSGPPKK42 pKa = 10.18TITSKK47 pKa = 10.91VGTPTTITNIAATQWRR63 pKa = 11.84ITGLGAYY70 pKa = 9.58LNEE73 pKa = 4.62SVTIDD78 pKa = 3.19IPEE81 pKa = 4.5AGGDD85 pKa = 3.34VTDD88 pKa = 5.47RR89 pKa = 11.84IKK91 pKa = 10.98AAISIPPGTPVSTLVQAAKK110 pKa = 9.11TATEE114 pKa = 3.76IALDD118 pKa = 3.86EE119 pKa = 4.79LEE121 pKa = 4.5VPSKK125 pKa = 10.8EE126 pKa = 4.28VLDD129 pKa = 3.66ATYY132 pKa = 10.77VRR134 pKa = 11.84TVNGVPVDD142 pKa = 3.84PVTGNVTVDD151 pKa = 3.03AVGGVEE157 pKa = 4.08VGPDD161 pKa = 3.26EE162 pKa = 4.66TDD164 pKa = 2.94TDD166 pKa = 3.17VWTNDD171 pKa = 2.96NPEE174 pKa = 4.26VVTAIYY180 pKa = 10.03GATADD185 pKa = 3.78PTDD188 pKa = 4.02PDD190 pKa = 3.9VYY192 pKa = 11.14VIAEE196 pKa = 3.95

Molecular weight: 20.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8DCX9|A0A5P8DCX9_9CAUD Uncharacterized protein OS=Gordonia phage Mcklovin OX=2652881 GN=69 PE=4 SV=1
MM1 pKa = 6.76TTRR4 pKa = 11.84PCNQPGCAKK13 pKa = 10.27KK14 pKa = 10.43ARR16 pKa = 11.84TRR18 pKa = 11.84GMCPACYY25 pKa = 9.85EE26 pKa = 4.15RR27 pKa = 11.84DD28 pKa = 3.32RR29 pKa = 11.84TRR31 pKa = 11.84QKK33 pKa = 11.28AYY35 pKa = 10.42GRR37 pKa = 11.84WEE39 pKa = 4.18SQHH42 pKa = 7.38VDD44 pKa = 3.15AQPVRR49 pKa = 11.84DD50 pKa = 4.76HH51 pKa = 6.07IQKK54 pKa = 10.23LRR56 pKa = 11.84DD57 pKa = 3.3AGISNKK63 pKa = 8.93RR64 pKa = 11.84LRR66 pKa = 11.84EE67 pKa = 3.98LTGVSTNTIQVLMTGRR83 pKa = 11.84PEE85 pKa = 4.34RR86 pKa = 11.84GHH88 pKa = 6.65GPTKK92 pKa = 10.23KK93 pKa = 10.16VLRR96 pKa = 11.84RR97 pKa = 11.84TADD100 pKa = 3.96RR101 pKa = 11.84ILAVPVPEE109 pKa = 3.94IAFAIASPGRR119 pKa = 11.84IVPALGTTRR128 pKa = 11.84RR129 pKa = 11.84LQALVANGYY138 pKa = 7.11SQRR141 pKa = 11.84EE142 pKa = 3.84ICRR145 pKa = 11.84RR146 pKa = 11.84LGWAWQGNATALFLGRR162 pKa = 11.84AEE164 pKa = 4.05YY165 pKa = 10.67VVARR169 pKa = 11.84RR170 pKa = 11.84ARR172 pKa = 11.84EE173 pKa = 3.81VAHH176 pKa = 7.16LFTQLQMVPGTDD188 pKa = 2.72VRR190 pKa = 11.84ARR192 pKa = 11.84NRR194 pKa = 11.84GRR196 pKa = 11.84AHH198 pKa = 6.64GWAPPLDD205 pKa = 3.83WDD207 pKa = 4.52EE208 pKa = 5.93DD209 pKa = 4.66LIDD212 pKa = 5.54DD213 pKa = 5.13PNATPAATEE222 pKa = 3.62QDD224 pKa = 3.9RR225 pKa = 11.84PRR227 pKa = 11.84MSTTLDD233 pKa = 3.12EE234 pKa = 5.08FEE236 pKa = 3.88WLLRR240 pKa = 11.84CGEE243 pKa = 4.16APEE246 pKa = 4.33VAAKK250 pKa = 9.79RR251 pKa = 11.84CRR253 pKa = 11.84VSLSTIRR260 pKa = 11.84TYY262 pKa = 9.66YY263 pKa = 10.58ARR265 pKa = 11.84AGRR268 pKa = 11.84DD269 pKa = 3.42LPAQLAPAAPLPVPYY284 pKa = 10.49SRR286 pKa = 11.84TRR288 pKa = 11.84RR289 pKa = 11.84SQVASS294 pKa = 3.5

Molecular weight: 32.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

17010

36

2530

220.9

24.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.711 ± 0.66

1.029 ± 0.155

6.996 ± 0.416

5.626 ± 0.296

2.54 ± 0.188

8.319 ± 0.4

2.105 ± 0.222

4.444 ± 0.183

3.192 ± 0.196

7.989 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.169

2.963 ± 0.217

5.585 ± 0.27

3.61 ± 0.288

8.019 ± 0.387

5.362 ± 0.198

7.031 ± 0.241

7.031 ± 0.184

1.999 ± 0.156

2.275 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski