Wenzhouxiangella sp. XN24
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3004 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M1Y5S4|A0A6M1Y5S4_9GAMM MMPL family transporter OS=Wenzhouxiangella sp. XN24 OX=2713569 GN=G6032_05130 PE=4 SV=1
MM1 pKa = 7.36 RR2 pKa = 11.84 TFASTLALAAALGLAAPAYY21 pKa = 9.94 AHH23 pKa = 6.9 EE24 pKa = 4.92 AGDD27 pKa = 3.34 WLVRR31 pKa = 11.84 FGGSNIDD38 pKa = 3.59 PKK40 pKa = 11.12 SDD42 pKa = 3.43 NGSLDD47 pKa = 3.22 LTAAGLEE54 pKa = 4.51 GVAVQDD60 pKa = 3.93 VNVDD64 pKa = 3.5 SQIGVTFNITYY75 pKa = 8.84 MYY77 pKa = 8.09 TANLGIEE84 pKa = 4.44 LLAALPYY91 pKa = 10.27 EE92 pKa = 4.38 HH93 pKa = 7.59 DD94 pKa = 3.84 IEE96 pKa = 4.74 VAGLPGTAATVTHH109 pKa = 6.92 LPPTLSLQYY118 pKa = 10.72 HH119 pKa = 6.1 FMPDD123 pKa = 3.08 AVFQPYY129 pKa = 9.83 VGAGLNLTWFLDD141 pKa = 3.74 DD142 pKa = 4.14 SKK144 pKa = 11.86 EE145 pKa = 4.1 EE146 pKa = 4.06 GLVAADD152 pKa = 4.02 GLLGATTGLDD162 pKa = 3.23 VDD164 pKa = 4.24 STSVGLAAQVGFDD177 pKa = 4.2 YY178 pKa = 10.73 IVNDD182 pKa = 3.07 TWFINFDD189 pKa = 3.67 VRR191 pKa = 11.84 WIDD194 pKa = 2.99 ISTDD198 pKa = 2.85 ATLTVDD204 pKa = 3.57 GADD207 pKa = 3.41 FSTAKK212 pKa = 10.37 VDD214 pKa = 3.21 IDD216 pKa = 4.25 PIVYY220 pKa = 9.88 GLHH223 pKa = 5.24 IGYY226 pKa = 9.94 RR227 pKa = 11.84 FF228 pKa = 3.18
Molecular weight: 24.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.961
Patrickios 1.1
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A6M1YDS7|A0A6M1YDS7_9GAMM Uncharacterized protein OS=Wenzhouxiangella sp. XN24 OX=2713569 GN=G6032_14655 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.28 QPSNLRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.16 RR14 pKa = 11.84 THH16 pKa = 5.96 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.24 NGRR28 pKa = 11.84 LVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.38 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LAVV44 pKa = 3.42
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3004
0
3004
1008693
37
3642
335.8
36.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.552 ± 0.063
0.835 ± 0.015
5.843 ± 0.038
6.488 ± 0.04
3.607 ± 0.03
8.735 ± 0.045
2.071 ± 0.022
4.468 ± 0.032
2.234 ± 0.033
10.951 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.021
2.28 ± 0.021
5.332 ± 0.033
3.066 ± 0.024
8.097 ± 0.048
5.026 ± 0.028
4.786 ± 0.034
7.669 ± 0.041
1.473 ± 0.019
2.244 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here