Amino acid dipepetide frequency for Escherichia phage K1H

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.597AlaAla: 10.597 ± 1.697
1.285AlaCys: 1.285 ± 0.353
6.824AlaAsp: 6.824 ± 0.86
6.583AlaGlu: 6.583 ± 1.077
4.576AlaPhe: 4.576 ± 0.62
7.707AlaGly: 7.707 ± 0.749
1.285AlaHis: 1.285 ± 0.43
5.379AlaIle: 5.379 ± 0.7
6.101AlaLys: 6.101 ± 0.85
8.59AlaLeu: 8.59 ± 0.863
1.766AlaMet: 1.766 ± 0.44
4.255AlaAsn: 4.255 ± 0.44
4.094AlaPro: 4.094 ± 0.536
3.532AlaGln: 3.532 ± 0.895
4.496AlaArg: 4.496 ± 0.618
5.861AlaSer: 5.861 ± 0.809
5.539AlaThr: 5.539 ± 0.761
7.306AlaVal: 7.306 ± 0.641
1.285AlaTrp: 1.285 ± 0.372
3.211AlaTyr: 3.211 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
1.044CysAla: 1.044 ± 0.261
0.0CysCys: 0.0 ± 0.0
1.204CysAsp: 1.204 ± 0.304
1.124CysGlu: 1.124 ± 0.408
0.321CysPhe: 0.321 ± 0.123
0.883CysGly: 0.883 ± 0.284
0.241CysHis: 0.241 ± 0.126
0.401CysIle: 0.401 ± 0.23
0.562CysLys: 0.562 ± 0.222
0.482CysLeu: 0.482 ± 0.247
0.08CysMet: 0.08 ± 0.072
0.321CysAsn: 0.321 ± 0.216
0.161CysPro: 0.161 ± 0.116
0.161CysGln: 0.161 ± 0.119
0.883CysArg: 0.883 ± 0.348
0.562CysSer: 0.562 ± 0.181
0.642CysThr: 0.642 ± 0.274
0.723CysVal: 0.723 ± 0.256
0.161CysTrp: 0.161 ± 0.117
0.482CysTyr: 0.482 ± 0.215
0.0CysXaa: 0.0 ± 0.0
Asp
6.503AspAla: 6.503 ± 0.676
0.803AspCys: 0.803 ± 0.228
5.379AspAsp: 5.379 ± 0.65
4.656AspGlu: 4.656 ± 0.592
2.328AspPhe: 2.328 ± 0.395
6.583AspGly: 6.583 ± 1.155
0.642AspHis: 0.642 ± 0.22
4.496AspIle: 4.496 ± 0.586
2.89AspLys: 2.89 ± 0.39
4.656AspLeu: 4.656 ± 0.734
1.445AspMet: 1.445 ± 0.339
2.248AspAsn: 2.248 ± 0.492
1.606AspPro: 1.606 ± 0.485
1.204AspGln: 1.204 ± 0.26
3.051AspArg: 3.051 ± 0.536
2.73AspSer: 2.73 ± 0.532
4.255AspThr: 4.255 ± 0.529
4.496AspVal: 4.496 ± 0.496
0.963AspTrp: 0.963 ± 0.238
2.408AspTyr: 2.408 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
6.101GluAla: 6.101 ± 0.949
0.642GluCys: 0.642 ± 0.313
3.452GluAsp: 3.452 ± 0.47
4.817GluGlu: 4.817 ± 0.981
2.649GluPhe: 2.649 ± 0.514
4.978GluGly: 4.978 ± 0.8
0.883GluHis: 0.883 ± 0.24
3.372GluIle: 3.372 ± 0.496
4.817GluLys: 4.817 ± 0.874
6.021GluLeu: 6.021 ± 0.61
2.087GluMet: 2.087 ± 0.396
2.168GluAsn: 2.168 ± 0.54
2.168GluPro: 2.168 ± 0.391
3.452GluGln: 3.452 ± 0.943
3.452GluArg: 3.452 ± 0.574
3.131GluSer: 3.131 ± 0.58
3.372GluThr: 3.372 ± 0.627
4.576GluVal: 4.576 ± 0.587
1.285GluTrp: 1.285 ± 0.336
2.489GluTyr: 2.489 ± 0.506
0.0GluXaa: 0.0 ± 0.0
Phe
2.408PheAla: 2.408 ± 0.447
0.321PheCys: 0.321 ± 0.199
3.452PheAsp: 3.452 ± 0.709
2.328PheGlu: 2.328 ± 0.439
1.044PhePhe: 1.044 ± 0.324
3.613PheGly: 3.613 ± 0.55
0.241PheHis: 0.241 ± 0.191
2.007PheIle: 2.007 ± 0.462
2.248PheLys: 2.248 ± 0.43
2.087PheLeu: 2.087 ± 0.36
0.723PheMet: 0.723 ± 0.221
1.846PheAsn: 1.846 ± 0.371
1.124PhePro: 1.124 ± 0.314
1.204PheGln: 1.204 ± 0.359
2.007PheArg: 2.007 ± 0.305
3.131PheSer: 3.131 ± 0.511
2.569PheThr: 2.569 ± 0.369
2.489PheVal: 2.489 ± 0.389
0.482PheTrp: 0.482 ± 0.19
1.285PheTyr: 1.285 ± 0.299
0.0PheXaa: 0.0 ± 0.0
Gly
7.547GlyAla: 7.547 ± 0.941
1.124GlyCys: 1.124 ± 0.324
4.656GlyAsp: 4.656 ± 0.817
4.978GlyGlu: 4.978 ± 0.57
3.532GlyPhe: 3.532 ± 0.583
5.218GlyGly: 5.218 ± 0.658
1.686GlyHis: 1.686 ± 0.372
2.97GlyIle: 2.97 ± 0.512
5.539GlyLys: 5.539 ± 0.887
5.459GlyLeu: 5.459 ± 0.533
2.087GlyMet: 2.087 ± 0.618
3.452GlyAsn: 3.452 ± 0.496
2.087GlyPro: 2.087 ± 0.398
3.292GlyGln: 3.292 ± 0.669
3.532GlyArg: 3.532 ± 0.555
5.379GlySer: 5.379 ± 0.661
4.255GlyThr: 4.255 ± 0.526
5.78GlyVal: 5.78 ± 0.976
1.525GlyTrp: 1.525 ± 0.346
2.97GlyTyr: 2.97 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.365HisAla: 1.365 ± 0.238
0.401HisCys: 0.401 ± 0.169
1.124HisAsp: 1.124 ± 0.268
0.803HisGlu: 0.803 ± 0.314
0.642HisPhe: 0.642 ± 0.226
1.365HisGly: 1.365 ± 0.405
0.963HisHis: 0.963 ± 0.279
0.883HisIle: 0.883 ± 0.216
1.124HisLys: 1.124 ± 0.382
1.124HisLeu: 1.124 ± 0.37
0.401HisMet: 0.401 ± 0.161
0.401HisAsn: 0.401 ± 0.183
0.883HisPro: 0.883 ± 0.325
0.562HisGln: 0.562 ± 0.185
1.285HisArg: 1.285 ± 0.34
0.803HisSer: 0.803 ± 0.267
0.803HisThr: 0.803 ± 0.249
1.285HisVal: 1.285 ± 0.4
0.0HisTrp: 0.0 ± 0.0
0.321HisTyr: 0.321 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
4.737IleAla: 4.737 ± 0.847
0.562IleCys: 0.562 ± 0.216
3.292IleAsp: 3.292 ± 0.484
3.372IleGlu: 3.372 ± 0.411
1.525IlePhe: 1.525 ± 0.394
2.81IleGly: 2.81 ± 0.401
0.642IleHis: 0.642 ± 0.245
2.73IleIle: 2.73 ± 0.328
3.372IleLys: 3.372 ± 0.594
3.131IleLeu: 3.131 ± 0.481
1.124IleMet: 1.124 ± 0.33
2.569IleAsn: 2.569 ± 0.447
2.89IlePro: 2.89 ± 0.467
1.766IleGln: 1.766 ± 0.447
1.766IleArg: 1.766 ± 0.353
4.175IleSer: 4.175 ± 0.573
4.175IleThr: 4.175 ± 0.584
4.175IleVal: 4.175 ± 0.5
0.963IleTrp: 0.963 ± 0.29
1.445IleTyr: 1.445 ± 0.509
0.0IleXaa: 0.0 ± 0.0
Lys
6.342LysAla: 6.342 ± 1.03
0.321LysCys: 0.321 ± 0.136
4.094LysAsp: 4.094 ± 0.599
4.576LysGlu: 4.576 ± 0.817
2.007LysPhe: 2.007 ± 0.323
3.452LysGly: 3.452 ± 0.467
1.525LysHis: 1.525 ± 0.348
1.606LysIle: 1.606 ± 0.414
2.73LysLys: 2.73 ± 0.521
4.897LysLeu: 4.897 ± 0.643
2.81LysMet: 2.81 ± 0.563
1.686LysAsn: 1.686 ± 0.363
2.408LysPro: 2.408 ± 0.435
2.168LysGln: 2.168 ± 0.572
4.094LysArg: 4.094 ± 0.684
3.292LysSer: 3.292 ± 0.578
4.175LysThr: 4.175 ± 0.601
3.452LysVal: 3.452 ± 0.643
0.642LysTrp: 0.642 ± 0.185
2.408LysTyr: 2.408 ± 0.404
0.0LysXaa: 0.0 ± 0.0
Leu
9.634LeuAla: 9.634 ± 0.897
0.883LeuCys: 0.883 ± 0.298
4.014LeuAsp: 4.014 ± 0.652
4.737LeuGlu: 4.737 ± 0.731
2.489LeuPhe: 2.489 ± 0.468
5.379LeuGly: 5.379 ± 0.524
1.285LeuHis: 1.285 ± 0.309
3.934LeuIle: 3.934 ± 0.426
4.094LeuLys: 4.094 ± 0.569
5.539LeuLeu: 5.539 ± 0.719
1.846LeuMet: 1.846 ± 0.339
3.613LeuAsn: 3.613 ± 0.514
3.854LeuPro: 3.854 ± 0.712
2.569LeuGln: 2.569 ± 0.492
4.335LeuArg: 4.335 ± 0.6
4.978LeuSer: 4.978 ± 0.601
6.423LeuThr: 6.423 ± 0.664
4.496LeuVal: 4.496 ± 0.743
0.963LeuTrp: 0.963 ± 0.336
2.649LeuTyr: 2.649 ± 0.546
0.0LeuXaa: 0.0 ± 0.0
Met
2.649MetAla: 2.649 ± 0.519
0.562MetCys: 0.562 ± 0.187
0.803MetAsp: 0.803 ± 0.292
1.044MetGlu: 1.044 ± 0.263
0.401MetPhe: 0.401 ± 0.201
2.007MetGly: 2.007 ± 0.356
0.161MetHis: 0.161 ± 0.106
1.525MetIle: 1.525 ± 0.382
1.846MetLys: 1.846 ± 0.456
1.846MetLeu: 1.846 ± 0.312
0.321MetMet: 0.321 ± 0.155
0.482MetAsn: 0.482 ± 0.236
1.686MetPro: 1.686 ± 0.35
0.723MetGln: 0.723 ± 0.254
1.285MetArg: 1.285 ± 0.267
2.328MetSer: 2.328 ± 0.304
1.606MetThr: 1.606 ± 0.293
1.686MetVal: 1.686 ± 0.398
0.161MetTrp: 0.161 ± 0.118
0.321MetTyr: 0.321 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
4.737AsnAla: 4.737 ± 0.734
0.482AsnCys: 0.482 ± 0.185
2.328AsnAsp: 2.328 ± 0.355
2.328AsnGlu: 2.328 ± 0.478
0.803AsnPhe: 0.803 ± 0.267
3.532AsnGly: 3.532 ± 0.681
0.401AsnHis: 0.401 ± 0.23
1.846AsnIle: 1.846 ± 0.459
1.846AsnLys: 1.846 ± 0.28
2.408AsnLeu: 2.408 ± 0.339
1.044AsnMet: 1.044 ± 0.364
2.248AsnAsn: 2.248 ± 0.402
1.846AsnPro: 1.846 ± 0.352
1.606AsnGln: 1.606 ± 0.354
1.927AsnArg: 1.927 ± 0.429
2.81AsnSer: 2.81 ± 0.32
2.649AsnThr: 2.649 ± 0.488
3.773AsnVal: 3.773 ± 0.53
0.883AsnTrp: 0.883 ± 0.214
1.365AsnTyr: 1.365 ± 0.308
0.0AsnXaa: 0.0 ± 0.0
Pro
3.292ProAla: 3.292 ± 0.486
0.562ProCys: 0.562 ± 0.215
2.89ProAsp: 2.89 ± 0.512
3.693ProGlu: 3.693 ± 0.466
1.766ProPhe: 1.766 ± 0.422
3.372ProGly: 3.372 ± 0.546
0.642ProHis: 0.642 ± 0.26
1.766ProIle: 1.766 ± 0.354
1.686ProLys: 1.686 ± 0.43
3.051ProLeu: 3.051 ± 0.602
0.883ProMet: 0.883 ± 0.319
1.766ProAsn: 1.766 ± 0.505
1.445ProPro: 1.445 ± 0.407
1.124ProGln: 1.124 ± 0.206
1.927ProArg: 1.927 ± 0.413
2.489ProSer: 2.489 ± 0.436
2.73ProThr: 2.73 ± 0.489
3.854ProVal: 3.854 ± 0.575
0.321ProTrp: 0.321 ± 0.174
0.883ProTyr: 0.883 ± 0.328
0.0ProXaa: 0.0 ± 0.0
Gln
4.014GlnAla: 4.014 ± 0.709
0.321GlnCys: 0.321 ± 0.162
1.525GlnAsp: 1.525 ± 0.312
1.766GlnGlu: 1.766 ± 0.422
1.285GlnPhe: 1.285 ± 0.267
1.927GlnGly: 1.927 ± 0.355
0.482GlnHis: 0.482 ± 0.204
2.328GlnIle: 2.328 ± 0.526
2.649GlnLys: 2.649 ± 0.578
3.854GlnLeu: 3.854 ± 0.654
1.204GlnMet: 1.204 ± 0.246
1.686GlnAsn: 1.686 ± 0.469
1.606GlnPro: 1.606 ± 0.322
2.73GlnGln: 2.73 ± 0.825
2.489GlnArg: 2.489 ± 0.412
1.846GlnSer: 1.846 ± 0.395
1.927GlnThr: 1.927 ± 0.482
2.649GlnVal: 2.649 ± 0.459
0.883GlnTrp: 0.883 ± 0.263
1.525GlnTyr: 1.525 ± 0.437
0.0GlnXaa: 0.0 ± 0.0
Arg
4.897ArgAla: 4.897 ± 0.603
0.562ArgCys: 0.562 ± 0.231
2.97ArgAsp: 2.97 ± 0.467
4.175ArgGlu: 4.175 ± 0.778
1.766ArgPhe: 1.766 ± 0.326
3.452ArgGly: 3.452 ± 0.559
1.686ArgHis: 1.686 ± 0.327
2.73ArgIle: 2.73 ± 0.484
3.372ArgLys: 3.372 ± 0.469
5.058ArgLeu: 5.058 ± 0.768
1.285ArgMet: 1.285 ± 0.308
2.328ArgAsn: 2.328 ± 0.399
1.606ArgPro: 1.606 ± 0.375
2.89ArgGln: 2.89 ± 0.532
4.656ArgArg: 4.656 ± 0.765
3.131ArgSer: 3.131 ± 0.448
2.73ArgThr: 2.73 ± 0.557
3.372ArgVal: 3.372 ± 0.428
0.723ArgTrp: 0.723 ± 0.246
1.285ArgTyr: 1.285 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
5.459SerAla: 5.459 ± 0.669
0.241SerCys: 0.241 ± 0.133
3.854SerAsp: 3.854 ± 0.525
3.211SerGlu: 3.211 ± 0.527
2.248SerPhe: 2.248 ± 0.449
7.466SerGly: 7.466 ± 0.816
1.044SerHis: 1.044 ± 0.313
2.569SerIle: 2.569 ± 0.695
3.934SerLys: 3.934 ± 0.604
4.496SerLeu: 4.496 ± 0.442
1.204SerMet: 1.204 ± 0.286
2.489SerAsn: 2.489 ± 0.621
2.73SerPro: 2.73 ± 0.522
2.248SerGln: 2.248 ± 0.456
3.934SerArg: 3.934 ± 0.57
3.934SerSer: 3.934 ± 1.065
4.817SerThr: 4.817 ± 0.485
4.656SerVal: 4.656 ± 0.534
0.642SerTrp: 0.642 ± 0.201
2.007SerTyr: 2.007 ± 0.538
0.0SerXaa: 0.0 ± 0.0
Thr
7.948ThrAla: 7.948 ± 1.013
0.482ThrCys: 0.482 ± 0.158
4.255ThrAsp: 4.255 ± 0.572
4.094ThrGlu: 4.094 ± 0.549
2.73ThrPhe: 2.73 ± 0.539
5.459ThrGly: 5.459 ± 0.837
0.883ThrHis: 0.883 ± 0.335
3.773ThrIle: 3.773 ± 0.47
3.613ThrLys: 3.613 ± 0.46
4.978ThrLeu: 4.978 ± 0.682
0.803ThrMet: 0.803 ± 0.231
1.766ThrAsn: 1.766 ± 0.405
4.335ThrPro: 4.335 ± 0.632
2.087ThrGln: 2.087 ± 0.418
2.489ThrArg: 2.489 ± 0.38
3.452ThrSer: 3.452 ± 0.494
4.255ThrThr: 4.255 ± 0.614
4.014ThrVal: 4.014 ± 0.63
0.963ThrTrp: 0.963 ± 0.261
2.248ThrTyr: 2.248 ± 0.479
0.0ThrXaa: 0.0 ± 0.0
Val
6.904ValAla: 6.904 ± 0.555
0.482ValCys: 0.482 ± 0.207
3.613ValAsp: 3.613 ± 0.444
4.737ValGlu: 4.737 ± 0.772
1.927ValPhe: 1.927 ± 0.389
4.576ValGly: 4.576 ± 0.557
0.803ValHis: 0.803 ± 0.219
4.817ValIle: 4.817 ± 0.743
3.773ValLys: 3.773 ± 0.53
5.78ValLeu: 5.78 ± 0.75
1.124ValMet: 1.124 ± 0.316
3.292ValAsn: 3.292 ± 0.634
2.007ValPro: 2.007 ± 0.445
3.051ValGln: 3.051 ± 0.528
4.416ValArg: 4.416 ± 0.543
5.539ValSer: 5.539 ± 0.664
5.058ValThr: 5.058 ± 0.824
4.335ValVal: 4.335 ± 0.665
0.883ValTrp: 0.883 ± 0.339
2.328ValTyr: 2.328 ± 0.51
0.0ValXaa: 0.0 ± 0.0
Trp
1.204TrpAla: 1.204 ± 0.452
0.161TrpCys: 0.161 ± 0.108
1.124TrpAsp: 1.124 ± 0.257
0.642TrpGlu: 0.642 ± 0.243
0.803TrpPhe: 0.803 ± 0.284
0.723TrpGly: 0.723 ± 0.283
0.401TrpHis: 0.401 ± 0.217
0.161TrpIle: 0.161 ± 0.107
0.723TrpLys: 0.723 ± 0.194
2.007TrpLeu: 2.007 ± 0.361
0.562TrpMet: 0.562 ± 0.199
0.642TrpAsn: 0.642 ± 0.288
0.562TrpPro: 0.562 ± 0.249
0.642TrpGln: 0.642 ± 0.229
0.803TrpArg: 0.803 ± 0.286
1.285TrpSer: 1.285 ± 0.368
0.482TrpThr: 0.482 ± 0.199
0.642TrpVal: 0.642 ± 0.176
0.08TrpTrp: 0.08 ± 0.085
0.401TrpTyr: 0.401 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.89TyrAla: 2.89 ± 0.631
0.241TyrCys: 0.241 ± 0.132
2.489TyrAsp: 2.489 ± 0.838
2.328TyrGlu: 2.328 ± 0.415
1.766TyrPhe: 1.766 ± 0.35
2.569TyrGly: 2.569 ± 0.461
0.642TyrHis: 0.642 ± 0.189
1.927TyrIle: 1.927 ± 0.303
1.766TyrLys: 1.766 ± 0.36
2.248TyrLeu: 2.248 ± 0.469
0.562TyrMet: 0.562 ± 0.198
1.606TyrAsn: 1.606 ± 0.311
1.044TyrPro: 1.044 ± 0.464
1.686TyrGln: 1.686 ± 0.328
1.846TyrArg: 1.846 ± 0.416
2.328TyrSer: 2.328 ± 0.457
2.168TyrThr: 2.168 ± 0.496
1.686TyrVal: 1.686 ± 0.411
0.241TyrTrp: 0.241 ± 0.117
1.044TyrTyr: 1.044 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski