Saimiriine betaherpesvirus 4
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8XSW0|G8XSW0_9BETA Capsid triplex subunit 1 OS=Saimiriine betaherpesvirus 4 OX=1535247 GN=UL46 PE=4 SV=1
MM1 pKa = 7.72 AAPLQCPVLVGMEE14 pKa = 4.81 DD15 pKa = 3.74 PDD17 pKa = 4.45 LPPPSYY23 pKa = 10.87 EE24 pKa = 3.89 EE25 pKa = 4.22 AMCSGNVTLTALPMSAMALPPPYY48 pKa = 10.09 EE49 pKa = 4.18 EE50 pKa = 4.42 QCLSTSLEE58 pKa = 3.75 MDD60 pKa = 5.23 PISEE64 pKa = 4.39 RR65 pKa = 11.84 DD66 pKa = 3.69 SNCPGRR72 pKa = 11.84 PTSNMEE78 pKa = 4.09 NLLLSDD84 pKa = 3.93 NFYY87 pKa = 11.37 SGLKK91 pKa = 7.0 WTLALFIMAVVGLIFIAIIFAVVISRR117 pKa = 11.84 RR118 pKa = 11.84 HH119 pKa = 4.17
Molecular weight: 12.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.994
IPC2_protein 4.291
IPC_protein 4.139
Toseland 3.973
ProMoST 4.24
Dawson 4.088
Bjellqvist 4.253
Wikipedia 3.973
Rodwell 3.973
Grimsley 3.897
Solomon 4.075
Lehninger 4.037
Nozaki 4.215
DTASelect 4.342
Thurlkill 3.999
EMBOSS 3.999
Sillero 4.253
Patrickios 0.846
IPC_peptide 4.088
IPC2_peptide 4.24
IPC2.peptide.svr19 4.153
Protein with the highest isoelectric point:
>tr|G8XT31|G8XT31_9BETA Protein UL145 OS=Saimiriine betaherpesvirus 4 OX=1535247 GN=UL145 PE=4 SV=1
MM1 pKa = 7.54 RR2 pKa = 11.84 VSIPYY7 pKa = 10.01 FKK9 pKa = 10.75 LLCLWNVLMIVTTSAHH25 pKa = 6.2 KK26 pKa = 9.96 NANRR30 pKa = 11.84 CVCGTSNLIKK40 pKa = 10.62 NVTHH44 pKa = 7.17 IKK46 pKa = 8.42 TQIKK50 pKa = 9.65 YY51 pKa = 9.39 IQPINANYY59 pKa = 9.09 HH60 pKa = 5.2 CKK62 pKa = 9.68 NRR64 pKa = 11.84 QVITTLLNNTKK75 pKa = 10.03 VCIDD79 pKa = 3.79 PEE81 pKa = 3.86 AVYY84 pKa = 10.21 IKK86 pKa = 10.51 RR87 pKa = 11.84 LLFMLMLKK95 pKa = 10.61 VNIQKK100 pKa = 10.74 KK101 pKa = 8.32 MVLDD105 pKa = 4.0 VYY107 pKa = 9.32 KK108 pKa = 10.5 TKK110 pKa = 10.72 VDD112 pKa = 4.06 FPGWGIQEE120 pKa = 3.96 IYY122 pKa = 10.04 KK123 pKa = 10.76 NLMLAKK129 pKa = 10.02 RR130 pKa = 11.84 RR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 PLRR136 pKa = 11.84 RR137 pKa = 11.84 KK138 pKa = 10.0 VLDD141 pKa = 4.02 LNTARR146 pKa = 11.84 LPRR149 pKa = 11.84 RR150 pKa = 11.84 KK151 pKa = 9.24 PRR153 pKa = 11.84 SHH155 pKa = 7.47 DD156 pKa = 3.21 VTIII160 pKa = 3.38
Molecular weight: 18.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.867
IPC_protein 10.35
Toseland 10.774
ProMoST 10.365
Dawson 10.877
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.316
Grimsley 10.906
Solomon 10.935
Lehninger 10.921
Nozaki 10.76
DTASelect 10.511
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.789
Patrickios 11.038
IPC_peptide 10.95
IPC2_peptide 9.487
IPC2.peptide.svr19 8.465
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
140
0
140
52840
58
2109
377.4
42.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.543 ± 0.188
2.672 ± 0.121
5.055 ± 0.139
5.515 ± 0.14
4.534 ± 0.119
4.54 ± 0.178
2.721 ± 0.113
5.838 ± 0.177
4.383 ± 0.15
10.5 ± 0.182
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.466 ± 0.1
4.326 ± 0.152
4.595 ± 0.157
3.516 ± 0.128
6.321 ± 0.201
7.935 ± 0.206
7.165 ± 0.256
7.129 ± 0.205
1.327 ± 0.105
3.917 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here