Raptor adenovirus 1
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4MI13|F4MI13_9ADEN Uncharacterized protein OS=Raptor adenovirus 1 OX=1520002 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 5.75 PRR4 pKa = 11.84 DD5 pKa = 3.91 LLDD8 pKa = 3.97 DD9 pKa = 4.22 AEE11 pKa = 4.39 RR12 pKa = 11.84 TQPVQDD18 pKa = 3.78 NKK20 pKa = 10.39 VSAVTKK26 pKa = 9.79 FYY28 pKa = 11.14 SGTKK32 pKa = 8.65 RR33 pKa = 11.84 TFPLLSEE40 pKa = 4.41 DD41 pKa = 3.37 AFIFHH46 pKa = 6.1 FHH48 pKa = 6.81 CGEE51 pKa = 3.97 TDD53 pKa = 3.9 CILIEE58 pKa = 4.28 NYY60 pKa = 8.22 GTYY63 pKa = 10.61 KK64 pKa = 10.42 LSEE67 pKa = 4.37 YY68 pKa = 11.17 NFGTLLTDD76 pKa = 3.75 LGGIVLEE83 pKa = 5.3 FKK85 pKa = 11.11 LPMHH89 pKa = 7.14 SMFQAALACKK99 pKa = 9.51 WPTFDD104 pKa = 4.82 FEE106 pKa = 4.99 YY107 pKa = 10.89 VVNVYY112 pKa = 10.54 QDD114 pKa = 3.28 MRR116 pKa = 11.84 VAIYY120 pKa = 8.26 EE121 pKa = 4.3 LPSTDD126 pKa = 2.48 YY127 pKa = 9.4 WAPVLSWFAWQCLTNGISFFDD148 pKa = 4.0 FVKK151 pKa = 10.89 LLGEE155 pKa = 3.91 QTGDD159 pKa = 3.32 YY160 pKa = 9.12 GTVFAA165 pKa = 6.14
Molecular weight: 18.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.18
IPC2_protein 4.457
IPC_protein 4.368
Toseland 4.202
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.482
Wikipedia 4.24
Rodwell 4.215
Grimsley 4.113
Solomon 4.329
Lehninger 4.291
Nozaki 4.444
DTASelect 4.647
Thurlkill 4.228
EMBOSS 4.253
Sillero 4.495
Patrickios 1.163
IPC_peptide 4.329
IPC2_peptide 4.482
IPC2.peptide.svr19 4.372
Protein with the highest isoelectric point:
>tr|F4MI08|F4MI08_9ADEN PVIII protein OS=Raptor adenovirus 1 OX=1520002 PE=4 SV=1
MM1 pKa = 7.67 LSFFHH6 pKa = 7.11 SPVKK10 pKa = 10.22 VVKK13 pKa = 10.19 AAEE16 pKa = 3.8 NSQIKK21 pKa = 10.71 GEE23 pKa = 3.89 ASQIRR28 pKa = 11.84 SITRR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 9.45 RR35 pKa = 11.84 RR36 pKa = 11.84 IPGSYY41 pKa = 10.78 ANWKK45 pKa = 9.7 QYY47 pKa = 11.03 GGDD50 pKa = 3.45 VKK52 pKa = 10.81 EE53 pKa = 4.93 AIAVVGRR60 pKa = 11.84 QPVCVRR66 pKa = 11.84 RR67 pKa = 11.84 YY68 pKa = 10.22 LIAKK72 pKa = 8.57 GIYY75 pKa = 9.47 IPPSTINYY83 pKa = 8.08 YY84 pKa = 8.95 VNKK87 pKa = 10.07 HH88 pKa = 4.69 FAEE91 pKa = 4.45 EE92 pKa = 4.06 NSKK95 pKa = 8.86 FTLFF99 pKa = 3.99
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.75
IPC_protein 10.043
Toseland 10.321
ProMoST 9.984
Dawson 10.496
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.95
Grimsley 10.57
Solomon 10.526
Lehninger 10.496
Nozaki 10.306
DTASelect 10.16
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.409
Patrickios 10.657
IPC_peptide 10.526
IPC2_peptide 8.916
IPC2.peptide.svr19 8.681
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
8162
58
1110
354.9
40.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.807 ± 0.479
1.789 ± 0.332
4.95 ± 0.362
5.648 ± 0.507
4.876 ± 0.299
5.134 ± 0.529
1.801 ± 0.184
6.322 ± 0.401
5.342 ± 0.543
9.458 ± 0.496
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.12 ± 0.135
6.261 ± 0.492
5.709 ± 0.303
4.631 ± 0.328
5.036 ± 0.484
7.094 ± 0.317
6.702 ± 0.442
6.052 ± 0.404
1.103 ± 0.137
4.166 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here