Streptococcus phage 53

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5PRU4|A0A1S5PRU4_9CAUD Scaffolding protein OS=Streptococcus phage 53 OX=1718280 GN=sp53_6 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.65IEE28 pKa = 3.9SARR31 pKa = 11.84QYY33 pKa = 10.3VVNSVGDD40 pKa = 3.54DD41 pKa = 3.37PKK43 pKa = 10.95FYY45 pKa = 11.07NLDD48 pKa = 3.41SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5PRV5|A0A1S5PRV5_9CAUD Uncharacterized protein OS=Streptococcus phage 53 OX=1718280 GN=sp53_18 PE=4 SV=1
MM1 pKa = 7.65LEE3 pKa = 3.9YY4 pKa = 10.54LASFRR9 pKa = 11.84LNSRR13 pKa = 11.84TVTSTFSRR21 pKa = 11.84GFLFLQKK28 pKa = 10.4KK29 pKa = 9.89LKK31 pKa = 10.4

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

10386

31

1656

253.3

28.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.21 ± 0.542

0.655 ± 0.202

6.393 ± 0.258

6.567 ± 0.456

4.4 ± 0.198

6.749 ± 0.483

1.464 ± 0.114

6.846 ± 0.256

8.242 ± 0.522

7.915 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.198

6.461 ± 0.26

3.177 ± 0.233

3.938 ± 0.216

4.342 ± 0.356

6.509 ± 0.388

6.201 ± 0.327

6.056 ± 0.31

1.473 ± 0.166

3.938 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski