Flavobacteriaceae bacterium PRS1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M7KLP1|A0A3M7KLP1_9FLAO MBL fold metallo-hydrolase OS=Flavobacteriaceae bacterium PRS1 OX=2483459 GN=EB822_06625 PE=4 SV=1
MM1 pKa = 7.34SYY3 pKa = 11.18VYY5 pKa = 10.32GIHH8 pKa = 7.09IADD11 pKa = 4.07LDD13 pKa = 4.62GINGNDD19 pKa = 3.95IIASSAGDD27 pKa = 3.82GKK29 pKa = 10.7LVWYY33 pKa = 10.11ANNGDD38 pKa = 3.68GTFADD43 pKa = 4.74GVDD46 pKa = 3.64ILTGLLDD53 pKa = 4.23PGNIVTGKK61 pKa = 10.41LMLVILSII69 pKa = 4.31

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M7KLC4|A0A3M7KLC4_9FLAO Glycosyltransferase OS=Flavobacteriaceae bacterium PRS1 OX=2483459 GN=EB822_03550 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.03TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2108

0

2108

686236

22

2401

325.5

36.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.979 ± 0.054

0.786 ± 0.018

5.525 ± 0.044

6.402 ± 0.049

5.303 ± 0.043

6.35 ± 0.051

1.797 ± 0.022

8.928 ± 0.056

8.06 ± 0.061

9.161 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.205 ± 0.028

6.398 ± 0.058

3.285 ± 0.027

3.166 ± 0.033

3.362 ± 0.034

6.423 ± 0.04

5.755 ± 0.051

6.072 ± 0.039

1.047 ± 0.02

3.994 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski