Ichthyenterobacterium magnum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Ichthyenterobacterium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A420DLP3|A0A420DLP3_9FLAO Glutamine cyclotransferase OS=Ichthyenterobacterium magnum OX=1230530 GN=BXY80_1305 PE=4 SV=1
MM1 pKa = 7.03KK2 pKa = 10.09HH3 pKa = 6.46IYY5 pKa = 9.54TSIILFFFFQLSFGQIGFEE24 pKa = 4.73DD25 pKa = 3.81NYY27 pKa = 9.61ITNFNNSTYY36 pKa = 11.01NPVGTVKK43 pKa = 10.6HH44 pKa = 5.98FVDD47 pKa = 4.92FDD49 pKa = 4.41GDD51 pKa = 3.43GDD53 pKa = 4.87LDD55 pKa = 3.82VLLSFFNRR63 pKa = 11.84VLSWHH68 pKa = 6.22EE69 pKa = 4.31NIDD72 pKa = 3.45GYY74 pKa = 11.82GNYY77 pKa = 9.57SQQNILLDD85 pKa = 3.86LPGTGNIVFEE95 pKa = 4.61VFDD98 pKa = 4.07FDD100 pKa = 4.62YY101 pKa = 11.37DD102 pKa = 3.66GDD104 pKa = 3.89EE105 pKa = 4.5DD106 pKa = 4.87VVLKK110 pKa = 10.37YY111 pKa = 10.86EE112 pKa = 4.17NVMYY116 pKa = 9.6WYY118 pKa = 10.29EE119 pKa = 4.57HH120 pKa = 6.82IDD122 pKa = 3.56GQNEE126 pKa = 3.97LNEE129 pKa = 4.36GVVILDD135 pKa = 4.86DD136 pKa = 3.95IQLGAYY142 pKa = 9.21KK143 pKa = 10.38FEE145 pKa = 6.41DD146 pKa = 3.39MDD148 pKa = 4.62GDD150 pKa = 3.68NDD152 pKa = 3.65KK153 pKa = 11.44DD154 pKa = 3.46IVFNSTTGNTKK165 pKa = 10.75LLMWSEE171 pKa = 3.98NDD173 pKa = 3.25GLNNFGPQQTIDD185 pKa = 3.31SATGFGGFKK194 pKa = 10.31FYY196 pKa = 11.32VDD198 pKa = 5.92DD199 pKa = 5.16IDD201 pKa = 6.4DD202 pKa = 5.01DD203 pKa = 5.55GDD205 pKa = 3.73MDD207 pKa = 4.27ILGAKK212 pKa = 9.49EE213 pKa = 3.86DD214 pKa = 4.15FVWYY218 pKa = 10.12EE219 pKa = 3.9NTDD222 pKa = 3.46GNGDD226 pKa = 3.83FSNEE230 pKa = 4.06HH231 pKa = 6.87IIQANVSANFSVVYY245 pKa = 10.67NRR247 pKa = 11.84GFVIADD253 pKa = 3.42YY254 pKa = 11.4DD255 pKa = 4.17DD256 pKa = 6.23DD257 pKa = 4.8GDD259 pKa = 4.48KK260 pKa = 11.15DD261 pKa = 3.84IITNEE266 pKa = 3.81YY267 pKa = 8.18QNYY270 pKa = 7.51MKK272 pKa = 10.02IYY274 pKa = 10.27EE275 pKa = 4.74NINDD279 pKa = 3.49ISFGSALLLSEE290 pKa = 4.14FHH292 pKa = 5.78NTYY295 pKa = 10.49GFAVMDD301 pKa = 3.96IDD303 pKa = 6.04LDD305 pKa = 4.03GDD307 pKa = 3.72QDD309 pKa = 3.81ILNGSSNGFYY319 pKa = 10.19WSEE322 pKa = 4.0NNNNTFLNSQSLFVDD337 pKa = 3.18NGGHH341 pKa = 5.99YY342 pKa = 9.6IEE344 pKa = 5.14KK345 pKa = 10.56LIDD348 pKa = 3.5IDD350 pKa = 5.55LDD352 pKa = 3.76GDD354 pKa = 4.34LDD356 pKa = 3.87ILVKK360 pKa = 10.72NYY362 pKa = 10.81SDD364 pKa = 3.69VLFKK368 pKa = 10.85INSNGTFEE376 pKa = 4.09EE377 pKa = 4.37TLPVFMQLSRR387 pKa = 11.84PKK389 pKa = 9.66EE390 pKa = 4.06VKK392 pKa = 10.39AADD395 pKa = 3.82LDD397 pKa = 4.07GDD399 pKa = 4.26LDD401 pKa = 4.65LDD403 pKa = 3.89IVATDD408 pKa = 3.99LYY410 pKa = 11.6GNIFWYY416 pKa = 10.99SNIDD420 pKa = 3.22EE421 pKa = 4.4MGYY424 pKa = 10.65FSVQRR429 pKa = 11.84YY430 pKa = 7.08IHH432 pKa = 7.2DD433 pKa = 3.68NSEE436 pKa = 3.92YY437 pKa = 11.02SDD439 pKa = 3.83DD440 pKa = 3.74QRR442 pKa = 11.84KK443 pKa = 9.83KK444 pKa = 10.82ISIGDD449 pKa = 3.86LDD451 pKa = 4.31GDD453 pKa = 3.98NDD455 pKa = 3.54NDD457 pKa = 3.95IISVSKK463 pKa = 10.35YY464 pKa = 9.55DD465 pKa = 4.89GRR467 pKa = 11.84ISWHH471 pKa = 6.59EE472 pKa = 3.93NLDD475 pKa = 3.82GQGDD479 pKa = 4.19FSNEE483 pKa = 3.69QIINSSLTDD492 pKa = 3.46QVHH495 pKa = 6.35SLDD498 pKa = 5.43LKK500 pKa = 11.31DD501 pKa = 4.25VDD503 pKa = 4.39NDD505 pKa = 3.42GDD507 pKa = 3.71NDD509 pKa = 3.88IVVGTHH515 pKa = 6.24YY516 pKa = 10.97GIYY519 pKa = 8.72WLKK522 pKa = 10.72NADD525 pKa = 3.56GLAGSWEE532 pKa = 4.11INQIGANVGSVSEE545 pKa = 4.5IEE547 pKa = 5.91LNDD550 pKa = 4.56LDD552 pKa = 4.76NDD554 pKa = 3.46NDD556 pKa = 3.77NDD558 pKa = 4.16VVCFDD563 pKa = 4.32GNAIVWFEE571 pKa = 3.97NVGGLGSYY579 pKa = 10.87GNMMPISSNTTYY591 pKa = 10.34FPKK594 pKa = 10.68GVFKK598 pKa = 10.77IFDD601 pKa = 3.9MNGDD605 pKa = 3.79GYY607 pKa = 11.43KK608 pKa = 10.53DD609 pKa = 3.64IIVSSRR615 pKa = 11.84DD616 pKa = 3.25NNNISFFEE624 pKa = 5.02HH625 pKa = 6.69IDD627 pKa = 3.51GNGAFEE633 pKa = 4.54DD634 pKa = 4.03AVIVVNDD641 pKa = 3.86ANGVSSIDD649 pKa = 3.59IADD652 pKa = 3.46VDD654 pKa = 3.99NDD656 pKa = 3.53GDD658 pKa = 4.51LDD660 pKa = 3.82ILAALADD667 pKa = 3.93FDD669 pKa = 4.15IVVWYY674 pKa = 9.94EE675 pKa = 3.88NLDD678 pKa = 3.67GQAEE682 pKa = 4.28AFGDD686 pKa = 3.99FNVITDD692 pKa = 4.06LQDD695 pKa = 3.34NTSSFVDD702 pKa = 3.22YY703 pKa = 10.53SGYY706 pKa = 10.15IVAVDD711 pKa = 3.37VNQDD715 pKa = 3.11NNIDD719 pKa = 4.11VISATQMYY727 pKa = 9.88HH728 pKa = 5.92NLVWSKK734 pKa = 11.06NLSLTNRR741 pKa = 11.84ISGFVNFTFDD751 pKa = 4.47LNGVGCDD758 pKa = 3.93FSNSIPVNNVLISTSNGVEE777 pKa = 4.27SKK779 pKa = 10.18ATFSLNNGHH788 pKa = 5.65YY789 pKa = 9.7QLYY792 pKa = 8.56PAQDD796 pKa = 3.56GNYY799 pKa = 6.86EE800 pKa = 4.22TQILTSLPLYY810 pKa = 10.45FEE812 pKa = 5.37SNPEE816 pKa = 3.94SEE818 pKa = 4.24TTEE821 pKa = 4.87FIGQGNTNEE830 pKa = 3.8VDD832 pKa = 4.08FCIEE836 pKa = 3.74PVGVVNDD843 pKa = 4.57LEE845 pKa = 4.92ISLYY849 pKa = 9.56PLSEE853 pKa = 3.89VRR855 pKa = 11.84PGYY858 pKa = 10.1DD859 pKa = 2.51VSYY862 pKa = 10.71QIVYY866 pKa = 10.33KK867 pKa = 10.88NVGTTIVNNGFITLDD882 pKa = 3.47YY883 pKa = 11.14DD884 pKa = 3.43NTKK887 pKa = 10.46INFQSSSEE895 pKa = 4.35SVFAQTSNSLAFQYY909 pKa = 11.28LDD911 pKa = 3.61LQPFEE916 pKa = 4.24IRR918 pKa = 11.84TIEE921 pKa = 4.91LNFSMNNIPSVMLGDD936 pKa = 3.27ILFFEE941 pKa = 4.79TEE943 pKa = 4.05ITPFTQDD950 pKa = 2.55EE951 pKa = 4.75TIYY954 pKa = 11.11NNSFNLQQTVIGSYY968 pKa = 10.55DD969 pKa = 3.51PNDD972 pKa = 3.23ISVLEE977 pKa = 4.26GEE979 pKa = 4.65QILLEE984 pKa = 4.57DD985 pKa = 3.88ADD987 pKa = 4.74KK988 pKa = 10.53YY989 pKa = 10.14LHH991 pKa = 6.46YY992 pKa = 10.47IIRR995 pKa = 11.84FQNTGTASAININVEE1010 pKa = 3.67NALDD1014 pKa = 5.29DD1015 pKa = 4.29KK1016 pKa = 11.64LDD1018 pKa = 3.58WTTMQLEE1025 pKa = 4.58SLSHH1029 pKa = 6.54AGRR1032 pKa = 11.84VEE1034 pKa = 3.71IRR1036 pKa = 11.84DD1037 pKa = 3.29GNMAKK1042 pKa = 10.52FIFNNIHH1049 pKa = 6.69LPDD1052 pKa = 3.8STNDD1056 pKa = 3.18EE1057 pKa = 4.65PNSHH1061 pKa = 6.43GYY1063 pKa = 8.83IAYY1066 pKa = 9.39KK1067 pKa = 10.19IKK1069 pKa = 10.36PKK1071 pKa = 10.65SDD1073 pKa = 3.48VEE1075 pKa = 4.33VGDD1078 pKa = 3.6IFYY1081 pKa = 8.64NTADD1085 pKa = 3.24IFFDD1089 pKa = 4.09FNPAIVTNTVSTEE1102 pKa = 3.65IVNALSVNEE1111 pKa = 4.1FEE1113 pKa = 4.93VKK1115 pKa = 10.42GFSIYY1120 pKa = 10.14PNPVNNTLTIEE1131 pKa = 4.41GKK1133 pKa = 9.99SALEE1137 pKa = 4.15SIAVYY1142 pKa = 10.16DD1143 pKa = 3.78VNGRR1147 pKa = 11.84EE1148 pKa = 4.14LNAIDD1153 pKa = 4.7IEE1155 pKa = 4.39NNQLQYY1161 pKa = 11.62LLDD1164 pKa = 3.83VSEE1167 pKa = 4.89LSQGIYY1173 pKa = 10.22FIEE1176 pKa = 4.17VQSDD1180 pKa = 3.34NFKK1183 pKa = 10.72SSKK1186 pKa = 10.64KK1187 pKa = 10.05FIKK1190 pKa = 10.17EE1191 pKa = 3.67

Molecular weight: 133.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A420DLE6|A0A420DLE6_9FLAO SnoaL-like domain-containing protein OS=Ichthyenterobacterium magnum OX=1230530 GN=BXY80_2046 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.66LSVSTEE48 pKa = 3.93TRR50 pKa = 11.84HH51 pKa = 6.24KK52 pKa = 10.66RR53 pKa = 3.3

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2733

0

2733

911278

25

2738

333.4

37.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.095 ± 0.045

0.788 ± 0.02

5.71 ± 0.042

6.278 ± 0.042

5.323 ± 0.043

6.045 ± 0.048

1.781 ± 0.022

8.304 ± 0.036

8.056 ± 0.076

9.272 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.024

6.798 ± 0.05

3.187 ± 0.031

3.324 ± 0.027

3.139 ± 0.031

6.545 ± 0.037

6.071 ± 0.054

6.12 ± 0.036

0.963 ± 0.016

4.15 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski