Ecklonia radiata-associated virus 3
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2CHQ9|A0A3G2CHQ9_9CIRC Rep-C OS=Ecklonia radiata-associated virus 3 OX=2480193 PE=4 SV=1
MM1 pKa = 7.57 AATAPSRR8 pKa = 11.84 QSRR11 pKa = 11.84 HH12 pKa = 5.31 RR13 pKa = 11.84 RR14 pKa = 11.84 WCFTLNSPVFSLNYY28 pKa = 10.1 YY29 pKa = 10.43 SILEE33 pKa = 4.21 NVSKK37 pKa = 10.85 RR38 pKa = 11.84 FVFGLEE44 pKa = 3.9 RR45 pKa = 11.84 APTTGRR51 pKa = 11.84 QHH53 pKa = 5.66 LQGYY57 pKa = 9.49 LEE59 pKa = 4.15 FANPSGFGRR68 pKa = 11.84 VSKK71 pKa = 10.34 LLPAHH76 pKa = 6.93 WEE78 pKa = 3.79 PARR81 pKa = 11.84 GSWKK85 pKa = 10.13 QNYY88 pKa = 8.65 DD89 pKa = 3.42 YY90 pKa = 10.86 CVKK93 pKa = 10.26 EE94 pKa = 4.15 GSYY97 pKa = 10.78 QSYY100 pKa = 10.81 GDD102 pKa = 3.85 WNSGVCDD109 pKa = 3.54 VSRR112 pKa = 11.84 SGVSNGKK119 pKa = 10.09 LSVSSIVRR127 pKa = 11.84 RR128 pKa = 11.84 LVYY131 pKa = 10.18 HH132 pKa = 5.32 QAEE135 pKa = 4.28 DD136 pKa = 4.51 VYY138 pKa = 11.4 LSWDD142 pKa = 3.51 YY143 pKa = 11.34 IRR145 pKa = 11.84 NKK147 pKa = 10.41 KK148 pKa = 10.64 SIDD151 pKa = 3.27 EE152 pKa = 4.06 LVYY155 pKa = 10.97 KK156 pKa = 10.62 FGQAKK161 pKa = 9.61 LRR163 pKa = 11.84 HH164 pKa = 5.4 EE165 pKa = 5.26 RR166 pKa = 11.84 YY167 pKa = 8.48 QQFYY171 pKa = 11.03 HH172 pKa = 6.69 SFLSDD177 pKa = 3.06 WQQEE181 pKa = 4.73 CILHH185 pKa = 6.64 LSEE188 pKa = 3.88 QGPRR192 pKa = 11.84 RR193 pKa = 11.84 VCWYY197 pKa = 10.1 YY198 pKa = 11.27 DD199 pKa = 3.4 VTGGTGKK206 pKa = 10.52 SFLANILFSCYY217 pKa = 10.05 NYY219 pKa = 10.67 EE220 pKa = 4.15 LFDD223 pKa = 5.56 GITSSRR229 pKa = 11.84 DD230 pKa = 3.0 ITLMLSDD237 pKa = 4.63 SFSGVCIDD245 pKa = 4.53 VTRR248 pKa = 11.84 DD249 pKa = 3.3 DD250 pKa = 4.63 NKK252 pKa = 10.73 LFSYY256 pKa = 9.06 STLEE260 pKa = 3.69 KK261 pKa = 10.41 LKK263 pKa = 10.94 NGFIMTGKK271 pKa = 7.89 YY272 pKa = 9.44 QGYY275 pKa = 9.57 KK276 pKa = 10.06 RR277 pKa = 11.84 LFAVKK282 pKa = 9.63 PVIVFANFEE291 pKa = 4.43 PDD293 pKa = 3.34 TTKK296 pKa = 10.8 LSSDD300 pKa = 3.67 RR301 pKa = 11.84 WCVHH305 pKa = 5.42 SLDD308 pKa = 4.38 YY309 pKa = 11.13 VSQEE313 pKa = 4.27 AKK315 pKa = 10.74 NPLPPTPFAPPAFSEE330 pKa = 4.34 EE331 pKa = 4.2 DD332 pKa = 3.53 PEE334 pKa = 4.19 AA335 pKa = 5.56
Molecular weight: 38.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.343
IPC2_protein 7.6
IPC_protein 7.483
Toseland 7.102
ProMoST 8.009
Dawson 8.141
Bjellqvist 8.492
Wikipedia 8.068
Rodwell 8.156
Grimsley 7.146
Solomon 8.229
Lehninger 8.229
Nozaki 8.609
DTASelect 8.244
Thurlkill 8.273
EMBOSS 8.346
Sillero 8.595
Patrickios 3.846
IPC_peptide 8.214
IPC2_peptide 7.571
IPC2.peptide.svr19 7.673
Protein with the highest isoelectric point:
>tr|A0A3G2CHQ9|A0A3G2CHQ9_9CIRC Rep-C OS=Ecklonia radiata-associated virus 3 OX=2480193 PE=4 SV=1
MM1 pKa = 7.57 AATAPSRR8 pKa = 11.84 QSRR11 pKa = 11.84 HH12 pKa = 5.31 RR13 pKa = 11.84 RR14 pKa = 11.84 WCFTLNSPVFSLNYY28 pKa = 10.1 YY29 pKa = 10.43 SILEE33 pKa = 4.21 NVSKK37 pKa = 10.85 RR38 pKa = 11.84 FVFGLEE44 pKa = 3.9 RR45 pKa = 11.84 APTTGRR51 pKa = 11.84 QHH53 pKa = 5.66 LQGYY57 pKa = 9.49 LEE59 pKa = 4.15 FANPSGFGRR68 pKa = 11.84 VSKK71 pKa = 10.34 LLPAHH76 pKa = 6.93 WEE78 pKa = 3.79 PARR81 pKa = 11.84 GSWKK85 pKa = 10.13 QNYY88 pKa = 8.65 DD89 pKa = 3.42 YY90 pKa = 10.86 CVKK93 pKa = 10.26 EE94 pKa = 4.15 GSYY97 pKa = 10.78 QSYY100 pKa = 10.81 GDD102 pKa = 3.85 WNSGVCDD109 pKa = 3.54 VSRR112 pKa = 11.84 SGVSNGKK119 pKa = 10.09 LSVSSIVRR127 pKa = 11.84 RR128 pKa = 11.84 LVYY131 pKa = 10.18 HH132 pKa = 5.32 QAEE135 pKa = 4.28 DD136 pKa = 4.51 VYY138 pKa = 11.4 LSWDD142 pKa = 3.51 YY143 pKa = 11.34 IRR145 pKa = 11.84 NKK147 pKa = 10.41 KK148 pKa = 10.64 SIDD151 pKa = 3.27 EE152 pKa = 4.06 LVYY155 pKa = 10.97 KK156 pKa = 10.62 FGQAKK161 pKa = 9.61 LRR163 pKa = 11.84 HH164 pKa = 5.4 EE165 pKa = 5.26 RR166 pKa = 11.84 YY167 pKa = 8.48 QQFYY171 pKa = 11.03 HH172 pKa = 6.69 SFLSDD177 pKa = 3.06 WQQEE181 pKa = 4.73 CILHH185 pKa = 6.64 LSEE188 pKa = 3.88 QGPRR192 pKa = 11.84 RR193 pKa = 11.84 VCWYY197 pKa = 10.1 YY198 pKa = 11.27 DD199 pKa = 3.4 VTGGTGKK206 pKa = 10.52 SFLANILFSCYY217 pKa = 10.05 NYY219 pKa = 10.67 EE220 pKa = 4.15 LFDD223 pKa = 5.56 GITSSRR229 pKa = 11.84 DD230 pKa = 3.0 ITLMLSDD237 pKa = 4.63 SFSGVCIDD245 pKa = 4.53 VTRR248 pKa = 11.84 DD249 pKa = 3.3 DD250 pKa = 4.63 NKK252 pKa = 10.73 LFSYY256 pKa = 9.06 STLEE260 pKa = 3.69 KK261 pKa = 10.41 LKK263 pKa = 10.94 NGFIMTGKK271 pKa = 7.89 YY272 pKa = 9.44 QGYY275 pKa = 9.57 KK276 pKa = 10.06 RR277 pKa = 11.84 LFAVKK282 pKa = 9.63 PVIVFANFEE291 pKa = 4.43 PDD293 pKa = 3.34 TTKK296 pKa = 10.8 LSSDD300 pKa = 3.67 RR301 pKa = 11.84 WCVHH305 pKa = 5.42 SLDD308 pKa = 4.38 YY309 pKa = 11.13 VSQEE313 pKa = 4.27 AKK315 pKa = 10.74 NPLPPTPFAPPAFSEE330 pKa = 4.34 EE331 pKa = 4.2 DD332 pKa = 3.53 PEE334 pKa = 4.19 AA335 pKa = 5.56
Molecular weight: 38.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.343
IPC2_protein 7.6
IPC_protein 7.483
Toseland 7.102
ProMoST 8.009
Dawson 8.141
Bjellqvist 8.492
Wikipedia 8.068
Rodwell 8.156
Grimsley 7.146
Solomon 8.229
Lehninger 8.229
Nozaki 8.609
DTASelect 8.244
Thurlkill 8.273
EMBOSS 8.346
Sillero 8.595
Patrickios 3.846
IPC_peptide 8.214
IPC2_peptide 7.571
IPC2.peptide.svr19 7.673
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
335
335
335
335.0
38.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.776 ± 0.0
2.388 ± 0.0
5.373 ± 0.0
5.075 ± 0.0
5.97 ± 0.0
6.269 ± 0.0
2.388 ± 0.0
3.284 ± 0.0
5.373 ± 0.0
8.358 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.896 ± 0.0
4.179 ± 0.0
4.776 ± 0.0
4.179 ± 0.0
6.269 ± 0.0
11.045 ± 0.0
4.179 ± 0.0
6.567 ± 0.0
2.388 ± 0.0
6.269 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here