Escherichia phage K1ind1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; Escherichia virus K1ind1

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2XJJ3|D2XJJ3_9CAUD Uncharacterized protein OS=Escherichia phage K1ind1 OX=698488 PE=4 SV=1
MM1 pKa = 7.76SDD3 pKa = 3.72LYY5 pKa = 10.8FPRR8 pKa = 11.84SLKK11 pKa = 10.58PIVSKK16 pKa = 10.6GYY18 pKa = 11.11SMTRR22 pKa = 11.84RR23 pKa = 11.84NNVWSVDD30 pKa = 3.73LAGGGVRR37 pKa = 11.84QGRR40 pKa = 11.84DD41 pKa = 2.51TYY43 pKa = 11.45YY44 pKa = 11.05DD45 pKa = 3.59VFPVSVTLITSAMGRR60 pKa = 11.84QAFLSFLEE68 pKa = 4.46KK69 pKa = 10.56VDD71 pKa = 4.19GGASSFWMAHH81 pKa = 5.89DD82 pKa = 4.3FGMGIEE88 pKa = 4.86DD89 pKa = 3.88YY90 pKa = 10.84QVTITSTIAEE100 pKa = 4.27STEE103 pKa = 4.87DD104 pKa = 4.05GINWTITFTATAEE117 pKa = 4.04KK118 pKa = 10.95SPFQDD123 pKa = 5.19LEE125 pKa = 4.27NQCLINNLPDD135 pKa = 4.42LYY137 pKa = 11.04GCYY140 pKa = 10.16GDD142 pKa = 4.96CLGSFLKK149 pKa = 10.31IYY151 pKa = 10.89ANYY154 pKa = 7.19EE155 pKa = 4.02TTFPRR160 pKa = 11.84IWSNEE165 pKa = 3.52GPAGYY170 pKa = 9.29PPINLLASTLDD181 pKa = 3.34SRR183 pKa = 11.84VVYY186 pKa = 10.23DD187 pKa = 4.5GPQVYY192 pKa = 10.4YY193 pKa = 10.37INRR196 pKa = 11.84NGNLVQSAANEE207 pKa = 4.02WPLTFIDD214 pKa = 4.87GSAVGRR220 pKa = 11.84VPPEE224 pKa = 3.51VSVTNRR230 pKa = 11.84FLYY233 pKa = 10.64SSNFSSSNWNKK244 pKa = 10.0GLVSVSVSEE253 pKa = 4.29TGVLTAAGTYY263 pKa = 10.49DD264 pKa = 3.4VTATTTSGNHH274 pKa = 7.46LINQTVDD281 pKa = 3.07VSVGVGDD288 pKa = 3.66MVTFSFFAKK297 pKa = 10.21ANGYY301 pKa = 9.34NFIRR305 pKa = 11.84MYY307 pKa = 10.64VRR309 pKa = 11.84EE310 pKa = 4.67LPTVLGSAVVDD321 pKa = 4.12LRR323 pKa = 11.84DD324 pKa = 3.31GSIYY328 pKa = 10.47SGPGGTDD335 pKa = 3.01VTDD338 pKa = 3.58VGGGWFRR345 pKa = 11.84ISEE348 pKa = 4.6TITATEE354 pKa = 4.63DD355 pKa = 2.89KK356 pKa = 10.49TMSIRR361 pKa = 11.84MDD363 pKa = 2.59AWIYY367 pKa = 10.54SDD369 pKa = 3.93GVTGSFAGDD378 pKa = 3.37GVSGIKK384 pKa = 10.44LSAATVEE391 pKa = 4.51VNQNTTSPVITDD403 pKa = 3.35AVVATRR409 pKa = 11.84TTASAKK415 pKa = 8.86VTMNGATSIDD425 pKa = 3.23IAYY428 pKa = 10.57SDD430 pKa = 3.69GSVVNVPSVDD440 pKa = 3.72GYY442 pKa = 11.97ASIPQADD449 pKa = 4.13SAWGSKK455 pKa = 10.25YY456 pKa = 8.24ITRR459 pKa = 11.84IDD461 pKa = 3.86FNVDD465 pKa = 2.67GG466 pKa = 4.77

Molecular weight: 50.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2XJK0|D2XJK0_9CAUD Putative DNA polymerase OS=Escherichia phage K1ind1 OX=698488 PE=3 SV=1
MM1 pKa = 7.77KK2 pKa = 9.71EE3 pKa = 4.14ALPKK7 pKa = 10.57LSQKK11 pKa = 9.0TLTGSPSVIFSRR23 pKa = 11.84VSAAGLSRR31 pKa = 11.84SDD33 pKa = 3.17LRR35 pKa = 11.84EE36 pKa = 3.59FRR38 pKa = 11.84KK39 pKa = 9.09MRR41 pKa = 11.84LSGRR45 pKa = 11.84EE46 pKa = 3.5ARR48 pKa = 11.84LANRR52 pKa = 11.84LARR55 pKa = 11.84RR56 pKa = 11.84EE57 pKa = 4.31SNSDD61 pKa = 2.97SSTLDD66 pKa = 3.31TLHH69 pKa = 6.73LCSSTSSASAALQSSLGNRR88 pKa = 11.84LRR90 pKa = 11.84QQLQNRR96 pKa = 11.84GCAIYY101 pKa = 10.63RR102 pKa = 11.84FTWKK106 pKa = 10.52EE107 pKa = 3.48KK108 pKa = 6.65TTPSGLPYY116 pKa = 9.93FQLVASAHH124 pKa = 4.83RR125 pKa = 11.84TKK127 pKa = 10.84EE128 pKa = 4.1SGSSLVRR135 pKa = 11.84TNWPTPTANDD145 pKa = 3.38YY146 pKa = 10.56RR147 pKa = 11.84GSGEE151 pKa = 4.02TVIRR155 pKa = 11.84KK156 pKa = 9.32DD157 pKa = 3.34GKK159 pKa = 10.49DD160 pKa = 3.04RR161 pKa = 11.84TFDD164 pKa = 3.46RR165 pKa = 11.84LDD167 pKa = 3.27YY168 pKa = 10.57STEE171 pKa = 3.76QGLKK175 pKa = 7.4ITQPIRR181 pKa = 11.84ITASGQILTGSDD193 pKa = 2.85AGMEE197 pKa = 4.07NSGQLNPAHH206 pKa = 6.46SRR208 pKa = 11.84WLMGYY213 pKa = 8.97PPEE216 pKa = 4.38WDD218 pKa = 3.7DD219 pKa = 4.05CAVMAMPSSRR229 pKa = 11.84KK230 pKa = 9.53

Molecular weight: 25.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12555

56

851

246.2

27.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.98 ± 0.501

1.091 ± 0.142

6.197 ± 0.263

6.34 ± 0.494

3.863 ± 0.178

7.869 ± 0.291

1.728 ± 0.195

5.217 ± 0.152

5.504 ± 0.393

7.806 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.174

4.19 ± 0.26

3.903 ± 0.251

3.775 ± 0.351

5.368 ± 0.281

6.476 ± 0.518

6.571 ± 0.389

7.121 ± 0.333

1.33 ± 0.159

3.297 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski