Weissella phage phiYS61

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6PCD6|I6PCD6_9CAUD Uncharacterized protein OS=Weissella phage phiYS61 OX=1161906 GN=phiYS61_01 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.48NIKK5 pKa = 8.79TLEE8 pKa = 3.87EE9 pKa = 4.07LFTYY13 pKa = 10.37VRR15 pKa = 11.84DD16 pKa = 3.78NGDD19 pKa = 3.44VVGLALEE26 pKa = 4.34LTGFEE31 pKa = 4.44YY32 pKa = 10.99VDD34 pKa = 3.94KK35 pKa = 11.12FDD37 pKa = 3.69IDD39 pKa = 3.22EE40 pKa = 4.78MYY42 pKa = 10.25TVSQLIEE49 pKa = 4.37DD50 pKa = 3.57QSNIIDD56 pKa = 4.04ALRR59 pKa = 11.84KK60 pKa = 9.98AEE62 pKa = 4.47GYY64 pKa = 10.57EE65 pKa = 4.28DD66 pKa = 3.94GQVWSTGSVYY76 pKa = 10.72YY77 pKa = 10.17YY78 pKa = 10.53NYY80 pKa = 9.78WEE82 pKa = 4.75GGSLEE87 pKa = 4.09EE88 pKa = 4.4MEE90 pKa = 4.43EE91 pKa = 4.21EE92 pKa = 3.76AWDD95 pKa = 4.33WIVSDD100 pKa = 4.72YY101 pKa = 11.36DD102 pKa = 3.21IDD104 pKa = 4.26TIKK107 pKa = 10.79EE108 pKa = 4.11KK109 pKa = 11.0YY110 pKa = 10.15SEE112 pKa = 4.26LFF114 pKa = 3.43

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6PC17|I6PC17_9CAUD Uncharacterized protein OS=Weissella phage phiYS61 OX=1161906 GN=phiYS61_47 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84VKK4 pKa = 10.83GFNNYY9 pKa = 9.51EE10 pKa = 4.01LDD12 pKa = 4.92PINGTCYY19 pKa = 8.74NTRR22 pKa = 11.84TKK24 pKa = 10.73RR25 pKa = 11.84FVGNIDD31 pKa = 3.2KK32 pKa = 10.63RR33 pKa = 11.84DD34 pKa = 3.74GYY36 pKa = 11.58VSVTLFEE43 pKa = 5.29DD44 pKa = 3.18NRR46 pKa = 11.84KK47 pKa = 8.63RR48 pKa = 11.84TVKK51 pKa = 10.41LHH53 pKa = 5.4TLMAEE58 pKa = 4.09HH59 pKa = 7.39FIPNQKK65 pKa = 9.81FKK67 pKa = 10.68RR68 pKa = 11.84TVHH71 pKa = 6.65HH72 pKa = 6.69INHH75 pKa = 5.41VRR77 pKa = 11.84TDD79 pKa = 3.41NRR81 pKa = 11.84VSNLTWATDD90 pKa = 3.71SEE92 pKa = 4.44QHH94 pKa = 6.32DD95 pKa = 4.56KK96 pKa = 11.21EE97 pKa = 4.36WLLKK101 pKa = 7.84TSKK104 pKa = 10.36SIRR107 pKa = 11.84VFNDD111 pKa = 3.1TFSKK115 pKa = 10.57EE116 pKa = 3.78FMSIRR121 pKa = 11.84EE122 pKa = 4.1ASRR125 pKa = 11.84EE126 pKa = 3.98LGLNPSGLRR135 pKa = 11.84MVLKK139 pKa = 10.56GKK141 pKa = 9.98YY142 pKa = 8.92KK143 pKa = 9.15HH144 pKa = 5.95TGGYY148 pKa = 9.62RR149 pKa = 11.84ATLVDD154 pKa = 4.3KK155 pKa = 10.72EE156 pKa = 4.24NKK158 pKa = 9.59LRR160 pKa = 3.98

Molecular weight: 18.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

10015

31

731

208.6

23.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.599 ± 0.501

0.17 ± 0.07

6.34 ± 0.234

6.77 ± 0.487

4.264 ± 0.231

7.639 ± 0.568

1.787 ± 0.185

5.362 ± 0.219

6.59 ± 0.54

7.119 ± 0.428

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.696 ± 0.175

6.241 ± 0.289

4.034 ± 0.308

4.363 ± 0.247

3.924 ± 0.21

6.081 ± 0.293

6.65 ± 0.244

6.68 ± 0.269

1.558 ± 0.178

4.134 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski