Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174)

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Sinsheimervirus; Escherichia virus phiX174

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P69592|J_BPPHS DNA-binding protein J OS=Enterobacteria phage phiX174 (Isolate Sanger) OX=1217068 GN=J PE=1 SV=2
MM1 pKa = 7.07SQVTEE6 pKa = 3.79QSVRR10 pKa = 11.84FQTALASIKK19 pKa = 10.14LIQASAVLDD28 pKa = 3.76LTEE31 pKa = 6.22DD32 pKa = 3.72DD33 pKa = 5.73FDD35 pKa = 5.01FLTSNKK41 pKa = 8.24VWIATDD47 pKa = 3.5RR48 pKa = 11.84SRR50 pKa = 11.84ARR52 pKa = 11.84RR53 pKa = 11.84CVEE56 pKa = 3.39ACVYY60 pKa = 8.38GTLDD64 pKa = 3.27FVGYY68 pKa = 9.24PRR70 pKa = 11.84FPAPVEE76 pKa = 4.43FIAAVIAYY84 pKa = 7.7YY85 pKa = 8.79VHH87 pKa = 6.96PVNIQTACLIMEE99 pKa = 4.57GAEE102 pKa = 3.88FTEE105 pKa = 4.46NIINGVEE112 pKa = 4.11RR113 pKa = 11.84PVKK116 pKa = 10.15AAEE119 pKa = 4.15LFAFTLRR126 pKa = 11.84VRR128 pKa = 11.84AGNTDD133 pKa = 3.12VLTDD137 pKa = 3.27AEE139 pKa = 4.44EE140 pKa = 4.01NVRR143 pKa = 11.84QKK145 pKa = 10.84LRR147 pKa = 11.84AEE149 pKa = 4.41GVMM152 pKa = 4.04

Molecular weight: 16.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P69594|K_BPPHS Protein K OS=Enterobacteria phage phiX174 (Isolate Sanger) OX=1217068 GN=K PE=3 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.56GKK5 pKa = 9.7KK6 pKa = 9.51RR7 pKa = 11.84SGARR11 pKa = 11.84PGRR14 pKa = 11.84PQPLRR19 pKa = 11.84GTKK22 pKa = 9.66GKK24 pKa = 10.57RR25 pKa = 11.84KK26 pKa = 9.2GARR29 pKa = 11.84LWYY32 pKa = 10.58VGGQQFF38 pKa = 3.98

Molecular weight: 4.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

2327

38

513

211.5

23.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.638 ± 0.91

1.289 ± 0.276

6.059 ± 0.39

4.34 ± 0.603

4.641 ± 0.421

6.36 ± 0.718

1.762 ± 0.328

4.469 ± 0.345

6.317 ± 0.664

9.325 ± 0.945

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.266 ± 0.352

4.985 ± 0.306

3.911 ± 0.618

4.727 ± 0.637

6.102 ± 0.727

6.79 ± 0.422

6.403 ± 0.431

5.801 ± 0.583

1.332 ± 0.159

3.481 ± 0.445
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski