Erwinia phage phiEa104

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Kolesnikvirus; Erwinia virus Ea214

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5AFV7|E5AFV7_9CAUD Uncharacterized protein OS=Erwinia phage phiEa104 OX=925986 GN=P104_00260 PE=4 SV=1
MM1 pKa = 7.37LCVHH5 pKa = 6.44GTTLEE10 pKa = 4.03NFKK13 pKa = 10.87MMIANEE19 pKa = 4.32GKK21 pKa = 9.68PSGAWNVSEE30 pKa = 5.22CDD32 pKa = 3.09AATYY36 pKa = 8.16FYY38 pKa = 10.42PLDD41 pKa = 4.59KK42 pKa = 10.27IAKK45 pKa = 9.02AEE47 pKa = 4.08CLEE50 pKa = 4.47SGDD53 pKa = 3.99FDD55 pKa = 5.38SEE57 pKa = 4.84SEE59 pKa = 4.22YY60 pKa = 11.19QDD62 pKa = 5.8AILQAAKK69 pKa = 10.12RR70 pKa = 11.84HH71 pKa = 5.09ARR73 pKa = 11.84GNAMTTAAIAEE84 pKa = 4.47RR85 pKa = 11.84DD86 pKa = 3.38TKK88 pKa = 11.13LIVFLADD95 pKa = 3.44VPEE98 pKa = 4.69HH99 pKa = 6.58LLEE102 pKa = 6.12DD103 pKa = 5.39DD104 pKa = 4.21YY105 pKa = 12.15SCDD108 pKa = 3.28NMADD112 pKa = 3.01IASFIHH118 pKa = 6.78EE119 pKa = 4.9DD120 pKa = 3.55DD121 pKa = 4.92FKK123 pKa = 11.77VEE125 pKa = 4.21MIQRR129 pKa = 11.84IWQYY133 pKa = 10.4SFFGIFSPYY142 pKa = 9.48CVQPDD147 pKa = 3.72NPYY150 pKa = 8.12MVSMSYY156 pKa = 10.46HH157 pKa = 6.58LEE159 pKa = 3.72QMARR163 pKa = 11.84AIQNSNAYY171 pKa = 10.12LYY173 pKa = 9.13DD174 pKa = 4.14TIEE177 pKa = 4.08EE178 pKa = 4.27SLDD181 pKa = 3.35EE182 pKa = 4.65SEE184 pKa = 4.29EE185 pKa = 4.09QKK187 pKa = 11.23DD188 pKa = 4.07LEE190 pKa = 4.11FLINII195 pKa = 4.81

Molecular weight: 22.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5AFV2|E5AFV2_9CAUD Uncharacterized protein OS=Erwinia phage phiEa104 OX=925986 GN=P104_00210 PE=4 SV=1
MM1 pKa = 7.39QFTDD5 pKa = 4.59LRR7 pKa = 11.84AEE9 pKa = 4.11YY10 pKa = 10.28KK11 pKa = 10.14PLWWHH16 pKa = 7.08DD17 pKa = 3.38AGLSKK22 pKa = 9.91TKK24 pKa = 10.26SGYY27 pKa = 10.21GKK29 pKa = 10.24NIPTPWAVNWAGRR42 pKa = 11.84LRR44 pKa = 11.84RR45 pKa = 11.84IYY47 pKa = 8.61STCYY51 pKa = 9.61GNAASLHH58 pKa = 6.02INVNGKK64 pKa = 9.9KK65 pKa = 9.46IAVMM69 pKa = 4.27

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

118

0

118

24525

35

1157

207.8

23.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.894 ± 0.348

1.142 ± 0.1

6.418 ± 0.209

6.369 ± 0.239

4.118 ± 0.132

6.883 ± 0.289

1.969 ± 0.141

5.888 ± 0.146

6.981 ± 0.266

7.914 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.142

5.158 ± 0.187

3.148 ± 0.156

3.67 ± 0.17

4.387 ± 0.158

6.76 ± 0.243

6.267 ± 0.278

6.915 ± 0.19

1.264 ± 0.128

4.049 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski