Erwinia phage vB_EamP-S6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Waedenswilvirus; Erwinia virus S6

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0YQA8|G0YQA8_9CAUD Gp016 OS=Erwinia phage vB_EamP-S6 OX=1051675 PE=4 SV=1
MM1 pKa = 6.91GTGSIIHH8 pKa = 6.91LYY10 pKa = 10.14VLATMDD16 pKa = 3.83VQPTAPGYY24 pKa = 9.91DD25 pKa = 2.6AVMGYY30 pKa = 8.8TNLVWNNFPNDD41 pKa = 4.0PEE43 pKa = 4.91LSTLLHH49 pKa = 6.63DD50 pKa = 4.47TAQQSAIRR58 pKa = 11.84CTVLAMPTDD67 pKa = 3.53

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0YQJ4|G0YQJ4_9CAUD Gp102 OS=Erwinia phage vB_EamP-S6 OX=1051675 PE=4 SV=1
MM1 pKa = 7.54IATFPSDD8 pKa = 3.33AQAHH12 pKa = 4.73NFIRR16 pKa = 11.84VKK18 pKa = 10.55AGGRR22 pKa = 11.84FRR24 pKa = 11.84AVVVPLLYY32 pKa = 10.44RR33 pKa = 11.84SGLADD38 pKa = 3.93KK39 pKa = 8.46PHH41 pKa = 7.05CNGKK45 pKa = 9.38VAILTIEE52 pKa = 4.48FPRR55 pKa = 11.84RR56 pKa = 11.84GTLCC60 pKa = 3.69

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

23912

35

3541

207.9

23.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.932 ± 0.669

0.845 ± 0.157

5.964 ± 0.166

5.587 ± 0.242

3.362 ± 0.223

7.093 ± 0.3

1.786 ± 0.165

5.018 ± 0.257

5.512 ± 0.24

8.949 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.146

5.282 ± 0.322

3.969 ± 0.204

4.939 ± 0.314

4.613 ± 0.178

6.143 ± 0.255

6.582 ± 0.28

6.511 ± 0.158

1.493 ± 0.189

3.484 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski