Candidatus Saccharimonas aalborgensis

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria; Candidatus Saccharimonas

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1033 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4PW32|R4PW32_9BACT Peptide chain release factor 2 OS=Candidatus Saccharimonas aalborgensis OX=1332188 GN=prfB PE=3 SV=1
MM1 pKa = 7.37TMQTIKK7 pKa = 10.62RR8 pKa = 11.84VVLSLVIAAAPFFVVSPVSAAVDD31 pKa = 3.44TCTWTGAVDD40 pKa = 3.86NTWSNGGNWTGCDD53 pKa = 3.14NAGVPEE59 pKa = 4.59NGDD62 pKa = 3.29ALTFPEE68 pKa = 4.28VANNKK73 pKa = 9.75SMNNDD78 pKa = 3.25LVGLSVTGLSFTGTGYY94 pKa = 8.83TVGGNAFSISTVTALDD110 pKa = 3.25ATEE113 pKa = 4.4SVTINADD120 pKa = 2.77ITYY123 pKa = 8.52TAFNASITPSAGKK136 pKa = 7.82TVTINGVSNFTLGGGEE152 pKa = 4.18VNIGGSGRR160 pKa = 11.84SGTVDD165 pKa = 3.9FYY167 pKa = 12.08GNITGTAAQLVAVSGATAVVRR188 pKa = 11.84GAANTYY194 pKa = 8.19TAATVGAEE202 pKa = 4.22SNAHH206 pKa = 5.24FVCRR210 pKa = 11.84SLTCFGDD217 pKa = 3.56NANDD221 pKa = 3.5IYY223 pKa = 11.42SGGGVVEE230 pKa = 4.23LHH232 pKa = 5.16TTGTYY237 pKa = 10.39VNNIQTSVVTPDD249 pKa = 3.35TSSIWAYY256 pKa = 11.25DD257 pKa = 3.55DD258 pKa = 3.4VTLNGAITVNDD269 pKa = 3.8GLYY272 pKa = 10.47FGQGTNGKK280 pKa = 7.1TLAVDD285 pKa = 3.92GDD287 pKa = 4.11VNLAAEE293 pKa = 4.74DD294 pKa = 3.87LQISGMSRR302 pKa = 11.84AANVVINGVVSGNHH316 pKa = 6.39SIFVYY321 pKa = 10.73SSTLTLAGTNTYY333 pKa = 9.57TGATYY338 pKa = 8.2ATSNNATIEE347 pKa = 4.17VTNQSGLGTSGAGTQIGDD365 pKa = 3.62GDD367 pKa = 3.92SLEE370 pKa = 4.76FNFASAQTVSEE381 pKa = 4.16PLYY384 pKa = 11.22VMGTGVSGTGAVVQTGASTTLSGAIALAGDD414 pKa = 3.58TTFGVDD420 pKa = 3.43SNALFSAFMLSGVISGTGNITVTTSPTTVVANSSIQFTGASPNTYY465 pKa = 8.79TGKK468 pKa = 8.82LTVQGVRR475 pKa = 11.84FYY477 pKa = 10.19PSKK480 pKa = 10.13AASVVAVTGDD490 pKa = 3.38MDD492 pKa = 4.11VLASASKK499 pKa = 10.15PSTVEE504 pKa = 3.72TAFSEE509 pKa = 4.82SIADD513 pKa = 3.63TSHH516 pKa = 4.56VHH518 pKa = 5.85LVKK521 pKa = 10.88DD522 pKa = 4.34GSNKK526 pKa = 9.89ASLSIGSSATEE537 pKa = 3.96TIGYY541 pKa = 6.74VTGDD545 pKa = 3.56GEE547 pKa = 4.88LSVGANGAAVVLTSNADD564 pKa = 3.37YY565 pKa = 9.86TFSGTVSKK573 pKa = 10.5FSNFPAGDD581 pKa = 3.56SFVVKK586 pKa = 10.32KK587 pKa = 9.82GTGTATLTGGLDD599 pKa = 3.18TSYY602 pKa = 10.72IAGVAPVFGAQEE614 pKa = 3.97GSLILNGVFTEE625 pKa = 4.78AGTQVLSGATLKK637 pKa = 10.25GTGTVGAVTVDD648 pKa = 2.85NGGAVSVGNSPGCMTFGSLTLNSGSTYY675 pKa = 7.16TQEE678 pKa = 3.95ISGTTACTQYY688 pKa = 11.4DD689 pKa = 3.67QATVTGAAVLGNATLATTLSTNPADD714 pKa = 3.54GTVFTILTAASVSGTFNGLPDD735 pKa = 4.0GATVNVNGVTLRR747 pKa = 11.84INYY750 pKa = 7.47TATSVTLTKK759 pKa = 10.73VGGNLAPTGSSTAIYY774 pKa = 10.08GLLGLCMVLLSTLALRR790 pKa = 11.84RR791 pKa = 11.84YY792 pKa = 9.74RR793 pKa = 11.84FNNHH797 pKa = 4.62TNN799 pKa = 3.14

Molecular weight: 79.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4PYS9|R4PYS9_9BACT Uncharacterized protein OS=Candidatus Saccharimonas aalborgensis OX=1332188 GN=L336_0686 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.55LKK5 pKa = 8.66THH7 pKa = 6.88KK8 pKa = 9.69GTAKK12 pKa = 10.41RR13 pKa = 11.84IKK15 pKa = 9.53LTSTGKK21 pKa = 8.2LTRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AFGNHH31 pKa = 5.04MLSKK35 pKa = 10.15KK36 pKa = 10.01SKK38 pKa = 9.02SRR40 pKa = 11.84KK41 pKa = 7.48RR42 pKa = 11.84TINTSATVSGSMAKK56 pKa = 10.22NIKK59 pKa = 9.86RR60 pKa = 11.84VLGVV64 pKa = 3.17

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1033

0

1033

304259

27

1885

294.5

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.993 ± 0.084

0.685 ± 0.023

5.701 ± 0.061

5.715 ± 0.09

3.541 ± 0.051

7.13 ± 0.103

2.235 ± 0.038

6.498 ± 0.063

5.135 ± 0.077

9.269 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.037

3.686 ± 0.063

4.114 ± 0.055

3.825 ± 0.041

5.607 ± 0.067

6.715 ± 0.081

6.621 ± 0.096

7.605 ± 0.061

1.133 ± 0.029

3.444 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski