Aspergillus udagawae
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9999 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K8LMY5|A0A0K8LMY5_9EURO Isoflavone reductase homolog IRL OS=Aspergillus udagawae OX=91492 GN=AUD_7924 PE=3 SV=1
MM1 pKa = 7.13 VSPYY5 pKa = 8.29 GTNYY9 pKa = 9.56 FPALAAEE16 pKa = 4.38 PTDD19 pKa = 4.13 ATGHH23 pKa = 6.62 LYY25 pKa = 10.11 WSIINAWLLLTDD37 pKa = 4.57 GATGSEE43 pKa = 4.64 TPQPSQTIMLANWWWGLSTLSASCWFPCVIVLPPVTLPPMQAPVITSLIEE93 pKa = 4.11 GFPKK97 pKa = 10.38 TIMPPAAEE105 pKa = 4.39 TPTLIVDD112 pKa = 5.4 PITISGTPSILNAPTSTSVTIQFNYY137 pKa = 10.17 SAIILPPFKK146 pKa = 11.24 YY147 pKa = 9.83 MDD149 pKa = 5.34 DD150 pKa = 4.16 DD151 pKa = 5.23 DD152 pKa = 4.35 GTILGSSSSNCWFLYY167 pKa = 10.02 DD168 pKa = 4.08 WPLKK172 pKa = 10.28 IPSISISNSSLWPKK186 pKa = 10.56 LDD188 pKa = 3.52 TPQPPGLDD196 pKa = 3.32 NNNDD200 pKa = 3.66 DD201 pKa = 4.26 EE202 pKa = 4.92 EE203 pKa = 6.89 GDD205 pKa = 3.56 VHH207 pKa = 7.19 EE208 pKa = 4.29 YY209 pKa = 10.72 HH210 pKa = 7.15 LNNLLDD216 pKa = 5.73 DD217 pKa = 6.06 DD218 pKa = 6.37 DD219 pKa = 7.7 DD220 pKa = 7.66 DD221 pKa = 7.74 DD222 pKa = 7.71 DD223 pKa = 7.7 DD224 pKa = 7.44 DD225 pKa = 6.89 DD226 pKa = 6.61 DD227 pKa = 5.5 NNNHH231 pKa = 5.75 NLKK234 pKa = 10.15 LVSGTGSAWALSYY247 pKa = 10.03 LTVTNDD253 pKa = 3.06 PMFSAGVPVAVGGTYY268 pKa = 9.97 TPTSQYY274 pKa = 11.31 SIATKK279 pKa = 10.12 PGTSSSIAAITSPATSRR296 pKa = 11.84 LWPSISHH303 pKa = 6.15 SLSISSITNVSKK315 pKa = 10.88 SIRR318 pKa = 11.84 QQ319 pKa = 3.36
Molecular weight: 34.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.668
EMBOSS 3.834
Sillero 3.973
Patrickios 0.693
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A0K8L6U3|A0A0K8L6U3_9EURO Putative ATP-dependent RNA helicase PB1A10.06c OS=Aspergillus udagawae OX=91492 GN=AUD_3006 PE=4 SV=1
MM1 pKa = 7.67 SDD3 pKa = 3.26 SLRR6 pKa = 11.84 LGDD9 pKa = 3.47 HH10 pKa = 6.14 HH11 pKa = 7.37 RR12 pKa = 11.84 LRR14 pKa = 11.84 NIQRR18 pKa = 11.84 AVPSALRR25 pKa = 11.84 TAMRR29 pKa = 11.84 SQPFRR34 pKa = 11.84 LPAQTLTTSLSTPLRR49 pKa = 11.84 TFSSALRR56 pKa = 11.84 SQPTPLTQSLPSFRR70 pKa = 11.84 TSLTSASSLSFGLTQQTRR88 pKa = 11.84 SFSASASLAGRR99 pKa = 11.84 RR100 pKa = 11.84 ATYY103 pKa = 9.91 NPSRR107 pKa = 11.84 RR108 pKa = 11.84 VQKK111 pKa = 10.2 RR112 pKa = 11.84 RR113 pKa = 11.84 HH114 pKa = 5.33 GFLARR119 pKa = 11.84 LRR121 pKa = 11.84 SRR123 pKa = 11.84 GGRR126 pKa = 11.84 KK127 pKa = 8.87 VLQHH131 pKa = 6.19 RR132 pKa = 11.84 RR133 pKa = 11.84 AKK135 pKa = 9.96 GRR137 pKa = 11.84 KK138 pKa = 7.78 SLSWW142 pKa = 3.4
Molecular weight: 15.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 11.038
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.003
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9999
0
9999
5324289
66
6233
532.5
58.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.701 ± 0.022
1.241 ± 0.009
5.615 ± 0.016
6.139 ± 0.023
3.749 ± 0.013
6.775 ± 0.023
2.408 ± 0.008
4.957 ± 0.015
4.58 ± 0.021
9.183 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.115 ± 0.007
3.605 ± 0.012
5.952 ± 0.024
4.06 ± 0.017
6.169 ± 0.02
8.274 ± 0.03
5.879 ± 0.014
6.277 ± 0.016
1.479 ± 0.009
2.842 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here