Amino acid dipepetide frequency for Novosphingobium sp. Leaf2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.056AlaAla: 19.056 ± 0.207	1.147AlaCys: 1.147 ± 0.033	7.852AlaAsp: 7.852 ± 0.093	6.401AlaGlu: 6.401 ± 0.089	4.455AlaPhe: 4.455 ± 0.068	11.59AlaGly: 11.59 ± 0.117	2.724AlaHis: 2.724 ± 0.06	6.878AlaIle: 6.878 ± 0.082	4.046AlaLys: 4.046 ± 0.072	14.274AlaLeu: 14.274 ± 0.157	4.127AlaMet: 4.127 ± 0.078	3.241AlaAsn: 3.241 ± 0.06	6.644AlaPro: 6.644 ± 0.093	5.73AlaGln: 5.73 ± 0.084	9.864AlaArg: 9.864 ± 0.108	6.803AlaSer: 6.803 ± 0.089	6.5AlaThr: 6.5 ± 0.094	8.981AlaVal: 8.981 ± 0.103	1.751AlaTrp: 1.751 ± 0.042	2.701AlaTyr: 2.701 ± 0.043	0.003AlaXaa: 0.003 ± 0.002
Cys	1.105CysAla: 1.105 ± 0.034	0.096CysCys: 0.096 ± 0.011	0.555CysAsp: 0.555 ± 0.023	0.437CysGlu: 0.437 ± 0.021	0.284CysPhe: 0.284 ± 0.017	0.907CysGly: 0.907 ± 0.034	0.238CysHis: 0.238 ± 0.015	0.389CysIle: 0.389 ± 0.019	0.18CysLys: 0.18 ± 0.013	0.757CysLeu: 0.757 ± 0.026	0.195CysMet: 0.195 ± 0.012	0.239CysAsn: 0.239 ± 0.012	0.444CysPro: 0.444 ± 0.022	0.212CysGln: 0.212 ± 0.016	0.581CysArg: 0.581 ± 0.027	0.418CysSer: 0.418 ± 0.02	0.411CysThr: 0.411 ± 0.021	0.576CysVal: 0.576 ± 0.022	0.106CysTrp: 0.106 ± 0.009	0.158CysTyr: 0.158 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.282AspAla: 8.282 ± 0.097	0.488AspCys: 0.488 ± 0.022	3.421AspAsp: 3.421 ± 0.06	3.156AspGlu: 3.156 ± 0.052	2.149AspPhe: 2.149 ± 0.045	5.687AspGly: 5.687 ± 0.075	1.38AspHis: 1.38 ± 0.035	2.875AspIle: 2.875 ± 0.05	1.779AspLys: 1.779 ± 0.04	5.737AspLeu: 5.737 ± 0.083	1.434AspMet: 1.434 ± 0.038	1.411AspAsn: 1.411 ± 0.034	3.701AspPro: 3.701 ± 0.065	1.83AspGln: 1.83 ± 0.041	4.419AspArg: 4.419 ± 0.067	2.148AspSer: 2.148 ± 0.045	3.07AspThr: 3.07 ± 0.058	4.417AspVal: 4.417 ± 0.069	1.084AspTrp: 1.084 ± 0.033	1.557AspTyr: 1.557 ± 0.037	0.001AspXaa: 0.001 ± 0.001
Glu	7.009GluAla: 7.009 ± 0.089	0.34GluCys: 0.34 ± 0.02	2.703GluAsp: 2.703 ± 0.051	2.43GluGlu: 2.43 ± 0.055	1.43GluPhe: 1.43 ± 0.039	4.178GluGly: 4.178 ± 0.069	1.174GluHis: 1.174 ± 0.037	2.684GluIle: 2.684 ± 0.051	1.853GluLys: 1.853 ± 0.049	4.598GluLeu: 4.598 ± 0.067	1.289GluMet: 1.289 ± 0.036	1.403GluAsn: 1.403 ± 0.042	2.466GluPro: 2.466 ± 0.058	1.883GluGln: 1.883 ± 0.043	4.367GluArg: 4.367 ± 0.064	2.079GluSer: 2.079 ± 0.052	2.977GluThr: 2.977 ± 0.049	3.288GluVal: 3.288 ± 0.05	0.761GluTrp: 0.761 ± 0.026	0.971GluTyr: 0.971 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	5.039PheAla: 5.039 ± 0.067	0.346PheCys: 0.346 ± 0.016	2.648PheAsp: 2.648 ± 0.048	1.913PheGlu: 1.913 ± 0.043	1.25PhePhe: 1.25 ± 0.045	3.53PheGly: 3.53 ± 0.06	0.726PheHis: 0.726 ± 0.025	1.394PheIle: 1.394 ± 0.039	0.885PheLys: 0.885 ± 0.028	2.968PheLeu: 2.968 ± 0.06	0.765PheMet: 0.765 ± 0.026	1.046PheAsn: 1.046 ± 0.035	1.495PhePro: 1.495 ± 0.038	0.89PheGln: 0.89 ± 0.028	2.042PheArg: 2.042 ± 0.042	2.024PheSer: 2.024 ± 0.049	2.145PheThr: 2.145 ± 0.047	2.621PheVal: 2.621 ± 0.047	0.511PheTrp: 0.511 ± 0.021	0.947PheTyr: 0.947 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	10.265GlyAla: 10.265 ± 0.118	0.864GlyCys: 0.864 ± 0.03	4.763GlyAsp: 4.763 ± 0.076	4.513GlyGlu: 4.513 ± 0.058	3.529GlyPhe: 3.529 ± 0.063	7.858GlyGly: 7.858 ± 0.126	1.961GlyHis: 1.961 ± 0.045	4.48GlyIle: 4.48 ± 0.063	3.377GlyLys: 3.377 ± 0.058	8.812GlyLeu: 8.812 ± 0.101	2.463GlyMet: 2.463 ± 0.05	2.371GlyAsn: 2.371 ± 0.052	3.817GlyPro: 3.817 ± 0.06	3.104GlyGln: 3.104 ± 0.054	6.106GlyArg: 6.106 ± 0.078	4.846GlySer: 4.846 ± 0.071	5.239GlyThr: 5.239 ± 0.088	6.361GlyVal: 6.361 ± 0.084	1.644GlyTrp: 1.644 ± 0.046	2.455GlyTyr: 2.455 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.664HisAla: 2.664 ± 0.049	0.234HisCys: 0.234 ± 0.014	1.339HisAsp: 1.339 ± 0.034	1.065HisGlu: 1.065 ± 0.032	0.874HisPhe: 0.874 ± 0.027	2.081HisGly: 2.081 ± 0.048	0.547HisHis: 0.547 ± 0.026	0.923HisIle: 0.923 ± 0.027	0.453HisLys: 0.453 ± 0.02	1.952HisLeu: 1.952 ± 0.042	0.492HisMet: 0.492 ± 0.019	0.445HisAsn: 0.445 ± 0.021	1.381HisPro: 1.381 ± 0.039	0.569HisGln: 0.569 ± 0.023	1.48HisArg: 1.48 ± 0.041	0.938HisSer: 0.938 ± 0.032	0.909HisThr: 0.909 ± 0.028	1.597HisVal: 1.597 ± 0.036	0.365HisTrp: 0.365 ± 0.018	0.582HisTyr: 0.582 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.019IleAla: 8.019 ± 0.093	0.461IleCys: 0.461 ± 0.022	3.817IleAsp: 3.817 ± 0.061	3.254IleGlu: 3.254 ± 0.061	1.413IlePhe: 1.413 ± 0.042	4.893IleGly: 4.893 ± 0.076	0.881IleHis: 0.881 ± 0.029	1.874IleIle: 1.874 ± 0.043	1.221IleLys: 1.221 ± 0.033	3.725IleLeu: 3.725 ± 0.062	0.93IleMet: 0.93 ± 0.029	1.284IleAsn: 1.284 ± 0.035	2.263IlePro: 2.263 ± 0.048	1.15IleGln: 1.15 ± 0.035	2.907IleArg: 2.907 ± 0.057	2.523IleSer: 2.523 ± 0.048	2.701IleThr: 2.701 ± 0.048	4.203IleVal: 4.203 ± 0.067	0.552IleTrp: 0.552 ± 0.026	1.05IleTyr: 1.05 ± 0.033	0.001IleXaa: 0.001 ± 0.001
Lys	4.246LysAla: 4.246 ± 0.067	0.152LysCys: 0.152 ± 0.011	1.584LysAsp: 1.584 ± 0.043	1.131LysGlu: 1.131 ± 0.038	0.845LysPhe: 0.845 ± 0.027	2.764LysGly: 2.764 ± 0.053	0.515LysHis: 0.515 ± 0.021	1.381LysIle: 1.381 ± 0.041	0.978LysLys: 0.978 ± 0.036	3.012LysLeu: 3.012 ± 0.051	0.647LysMet: 0.647 ± 0.028	0.721LysAsn: 0.721 ± 0.026	1.99LysPro: 1.99 ± 0.045	0.951LysGln: 0.951 ± 0.031	2.153LysArg: 2.153 ± 0.047	1.508LysSer: 1.508 ± 0.042	1.707LysThr: 1.707 ± 0.049	2.333LysVal: 2.333 ± 0.05	0.374LysTrp: 0.374 ± 0.02	0.586LysTyr: 0.586 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	14.345LeuAla: 14.345 ± 0.138	0.84LeuCys: 0.84 ± 0.026	6.318LeuAsp: 6.318 ± 0.087	4.781LeuGlu: 4.781 ± 0.073	3.416LeuPhe: 3.416 ± 0.067	8.445LeuGly: 8.445 ± 0.089	1.934LeuHis: 1.934 ± 0.044	4.49LeuIle: 4.49 ± 0.07	2.815LeuLys: 2.815 ± 0.054	9.234LeuLeu: 9.234 ± 0.115	2.114LeuMet: 2.114 ± 0.044	2.37LeuAsn: 2.37 ± 0.047	5.536LeuPro: 5.536 ± 0.076	2.668LeuGln: 2.668 ± 0.054	6.908LeuArg: 6.908 ± 0.087	5.901LeuSer: 5.901 ± 0.082	5.629LeuThr: 5.629 ± 0.08	7.355LeuVal: 7.355 ± 0.091	1.14LeuTrp: 1.14 ± 0.038	1.924LeuTyr: 1.924 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.389MetAla: 3.389 ± 0.065	0.168MetCys: 0.168 ± 0.013	1.231MetAsp: 1.231 ± 0.031	1.043MetGlu: 1.043 ± 0.033	0.668MetPhe: 0.668 ± 0.028	1.967MetGly: 1.967 ± 0.044	0.447MetHis: 0.447 ± 0.02	1.335MetIle: 1.335 ± 0.037	0.881MetLys: 0.881 ± 0.033	2.598MetLeu: 2.598 ± 0.051	0.635MetMet: 0.635 ± 0.025	0.756MetAsn: 0.756 ± 0.03	1.591MetPro: 1.591 ± 0.045	0.793MetGln: 0.793 ± 0.029	1.865MetArg: 1.865 ± 0.047	1.413MetSer: 1.413 ± 0.034	1.791MetThr: 1.791 ± 0.038	1.698MetVal: 1.698 ± 0.042	0.213MetTrp: 0.213 ± 0.015	0.257MetTyr: 0.257 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.556AsnAla: 3.556 ± 0.055	0.238AsnCys: 0.238 ± 0.016	1.449AsnAsp: 1.449 ± 0.041	1.057AsnGlu: 1.057 ± 0.033	0.973AsnPhe: 0.973 ± 0.031	2.461AsnGly: 2.461 ± 0.053	0.478AsnHis: 0.478 ± 0.021	1.168AsnIle: 1.168 ± 0.035	0.641AsnLys: 0.641 ± 0.028	2.538AsnLeu: 2.538 ± 0.051	0.519AsnMet: 0.519 ± 0.02	0.653AsnAsn: 0.653 ± 0.029	1.858AsnPro: 1.858 ± 0.044	0.761AsnGln: 0.761 ± 0.028	1.83AsnArg: 1.83 ± 0.038	1.178AsnSer: 1.178 ± 0.035	1.329AsnThr: 1.329 ± 0.041	2.006AsnVal: 2.006 ± 0.049	0.422AsnTrp: 0.422 ± 0.02	0.702AsnTyr: 0.702 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	7.511ProAla: 7.511 ± 0.088	0.348ProCys: 0.348 ± 0.017	3.779ProAsp: 3.779 ± 0.063	3.235ProGlu: 3.235 ± 0.055	1.93ProPhe: 1.93 ± 0.05	4.966ProGly: 4.966 ± 0.078	1.119ProHis: 1.119 ± 0.027	2.39ProIle: 2.39 ± 0.046	1.428ProLys: 1.428 ± 0.039	5.028ProLeu: 5.028 ± 0.078	1.213ProMet: 1.213 ± 0.032	1.262ProAsn: 1.262 ± 0.035	2.779ProPro: 2.779 ± 0.069	1.973ProGln: 1.973 ± 0.046	3.183ProArg: 3.183 ± 0.068	2.754ProSer: 2.754 ± 0.046	2.616ProThr: 2.616 ± 0.053	4.397ProVal: 4.397 ± 0.066	0.693ProTrp: 0.693 ± 0.029	1.12ProTyr: 1.12 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.66GlnAla: 4.66 ± 0.086	0.241GlnCys: 0.241 ± 0.015	1.775GlnAsp: 1.775 ± 0.043	1.301GlnGlu: 1.301 ± 0.038	1.141GlnPhe: 1.141 ± 0.031	2.804GlnGly: 2.804 ± 0.051	0.678GlnHis: 0.678 ± 0.026	1.859GlnIle: 1.859 ± 0.041	0.865GlnLys: 0.865 ± 0.031	3.248GlnLeu: 3.248 ± 0.059	0.945GlnMet: 0.945 ± 0.026	0.818GlnAsn: 0.818 ± 0.032	1.928GlnPro: 1.928 ± 0.045	1.263GlnGln: 1.263 ± 0.041	2.559GlnArg: 2.559 ± 0.052	1.719GlnSer: 1.719 ± 0.039	1.749GlnThr: 1.749 ± 0.041	2.64GlnVal: 2.64 ± 0.049	0.5GlnTrp: 0.5 ± 0.02	0.685GlnTyr: 0.685 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.627ArgAla: 8.627 ± 0.108	0.535ArgCys: 0.535 ± 0.025	4.165ArgAsp: 4.165 ± 0.057	3.779ArgGlu: 3.779 ± 0.063	3.034ArgPhe: 3.034 ± 0.059	4.884ArgGly: 4.884 ± 0.063	1.745ArgHis: 1.745 ± 0.042	4.086ArgIle: 4.086 ± 0.062	2.191ArgLys: 2.191 ± 0.051	7.573ArgLeu: 7.573 ± 0.107	1.973ArgMet: 1.973 ± 0.045	1.887ArgAsn: 1.887 ± 0.043	3.548ArgPro: 3.548 ± 0.061	2.63ArgGln: 2.63 ± 0.052	5.412ArgArg: 5.412 ± 0.085	3.571ArgSer: 3.571 ± 0.059	3.599ArgThr: 3.599 ± 0.057	4.74ArgVal: 4.74 ± 0.064	1.14ArgTrp: 1.14 ± 0.038	1.925ArgTyr: 1.925 ± 0.044	0.002ArgXaa: 0.002 ± 0.001
Ser	6.653SerAla: 6.653 ± 0.084	0.42SerCys: 0.42 ± 0.019	3.028SerAsp: 3.028 ± 0.056	2.354SerGlu: 2.354 ± 0.05	1.913SerPhe: 1.913 ± 0.047	5.477SerGly: 5.477 ± 0.086	0.991SerHis: 0.991 ± 0.029	2.502SerIle: 2.502 ± 0.046	1.396SerLys: 1.396 ± 0.041	5.003SerLeu: 5.003 ± 0.066	1.204SerMet: 1.204 ± 0.031	1.34SerAsn: 1.34 ± 0.041	2.833SerPro: 2.833 ± 0.047	1.705SerGln: 1.705 ± 0.041	3.492SerArg: 3.492 ± 0.052	2.769SerSer: 2.769 ± 0.077	2.805SerThr: 2.805 ± 0.062	3.711SerVal: 3.711 ± 0.065	0.771SerTrp: 0.771 ± 0.028	1.346SerTyr: 1.346 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	6.683ThrAla: 6.683 ± 0.087	0.423ThrCys: 0.423 ± 0.019	2.974ThrAsp: 2.974 ± 0.052	2.188ThrGlu: 2.188 ± 0.048	1.985ThrPhe: 1.985 ± 0.045	5.598ThrGly: 5.598 ± 0.091	1.039ThrHis: 1.039 ± 0.034	2.955ThrIle: 2.955 ± 0.054	1.347ThrLys: 1.347 ± 0.036	5.93ThrLeu: 5.93 ± 0.081	1.238ThrMet: 1.238 ± 0.031	1.307ThrAsn: 1.307 ± 0.042	3.589ThrPro: 3.589 ± 0.058	1.721ThrGln: 1.721 ± 0.04	3.553ThrArg: 3.553 ± 0.056	2.792ThrSer: 2.792 ± 0.055	2.864ThrThr: 2.864 ± 0.063	4.506ThrVal: 4.506 ± 0.071	0.688ThrTrp: 0.688 ± 0.026	1.315ThrTyr: 1.315 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	9.384ValAla: 9.384 ± 0.106	0.6ValCys: 0.6 ± 0.025	4.326ValAsp: 4.326 ± 0.066	4.152ValGlu: 4.152 ± 0.064	2.437ValPhe: 2.437 ± 0.053	5.548ValGly: 5.548 ± 0.079	1.434ValHis: 1.434 ± 0.043	3.936ValIle: 3.936 ± 0.063	2.091ValLys: 2.091 ± 0.047	7.313ValLeu: 7.313 ± 0.093	1.786ValMet: 1.786 ± 0.045	2.094ValAsn: 2.094 ± 0.059	4.066ValPro: 4.066 ± 0.064	2.19ValGln: 2.19 ± 0.051	5.232ValArg: 5.232 ± 0.069	4.35ValSer: 4.35 ± 0.059	4.613ValThr: 4.613 ± 0.073	5.54ValVal: 5.54 ± 0.081	0.91ValTrp: 0.91 ± 0.033	1.356ValTyr: 1.356 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.405TrpAla: 1.405 ± 0.041	0.128TrpCys: 0.128 ± 0.011	0.743TrpAsp: 0.743 ± 0.029	0.553TrpGlu: 0.553 ± 0.024	0.581TrpPhe: 0.581 ± 0.022	0.966TrpGly: 0.966 ± 0.029	0.38TrpHis: 0.38 ± 0.02	0.683TrpIle: 0.683 ± 0.026	0.501TrpLys: 0.501 ± 0.019	1.765TrpLeu: 1.765 ± 0.044	0.354TrpMet: 0.354 ± 0.02	0.495TrpAsn: 0.495 ± 0.022	0.71TrpPro: 0.71 ± 0.027	0.629TrpGln: 0.629 ± 0.025	1.284TrpArg: 1.284 ± 0.034	0.838TrpSer: 0.838 ± 0.028	0.847TrpThr: 0.847 ± 0.03	0.819TrpVal: 0.819 ± 0.03	0.243TrpTrp: 0.243 ± 0.016	0.3TrpTyr: 0.3 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.868TyrAla: 2.868 ± 0.061	0.22TyrCys: 0.22 ± 0.014	1.554TyrAsp: 1.554 ± 0.043	1.141TyrGlu: 1.141 ± 0.036	0.877TyrPhe: 0.877 ± 0.029	2.221TyrGly: 2.221 ± 0.052	0.498TyrHis: 0.498 ± 0.02	0.839TyrIle: 0.839 ± 0.032	0.653TyrLys: 0.653 ± 0.027	2.1TyrLeu: 2.1 ± 0.053	0.417TyrMet: 0.417 ± 0.018	0.671TyrAsn: 0.671 ± 0.03	1.083TyrPro: 1.083 ± 0.03	0.725TyrGln: 0.725 ± 0.029	1.811TyrArg: 1.811 ± 0.04	1.138TyrSer: 1.138 ± 0.032	1.193TyrThr: 1.193 ± 0.033	1.569TyrVal: 1.569 ± 0.034	0.356TyrTrp: 0.356 ± 0.018	0.629TyrTyr: 0.629 ± 0.028	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3387 proteins (1086820 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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