Amino acid dipepetide frequency for Streptomyces sp. CB02923

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.329AlaAla: 23.329 ± 0.178	1.165AlaCys: 1.165 ± 0.024	8.869AlaAsp: 8.869 ± 0.07	8.766AlaGlu: 8.766 ± 0.081	3.566AlaPhe: 3.566 ± 0.041	14.13AlaGly: 14.13 ± 0.095	3.101AlaHis: 3.101 ± 0.038	2.982AlaIle: 2.982 ± 0.042	2.812AlaLys: 2.812 ± 0.046	14.827AlaLeu: 14.827 ± 0.117	2.475AlaMet: 2.475 ± 0.033	1.851AlaAsn: 1.851 ± 0.026	7.748AlaPro: 7.748 ± 0.075	3.858AlaGln: 3.858 ± 0.044	10.875AlaArg: 10.875 ± 0.083	5.692AlaSer: 5.692 ± 0.054	6.906AlaThr: 6.906 ± 0.057	12.561AlaVal: 12.561 ± 0.089	1.856AlaTrp: 1.856 ± 0.025	2.878AlaTyr: 2.878 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.225CysAla: 1.225 ± 0.023	0.096CysCys: 0.096 ± 0.008	0.497CysAsp: 0.497 ± 0.014	0.426CysGlu: 0.426 ± 0.013	0.233CysPhe: 0.233 ± 0.009	1.002CysGly: 1.002 ± 0.018	0.204CysHis: 0.204 ± 0.009	0.185CysIle: 0.185 ± 0.008	0.118CysLys: 0.118 ± 0.008	0.809CysLeu: 0.809 ± 0.017	0.136CysMet: 0.136 ± 0.007	0.123CysAsn: 0.123 ± 0.007	0.524CysPro: 0.524 ± 0.018	0.167CysGln: 0.167 ± 0.008	0.629CysArg: 0.629 ± 0.017	0.455CysSer: 0.455 ± 0.016	0.533CysThr: 0.533 ± 0.015	0.683CysVal: 0.683 ± 0.018	0.139CysTrp: 0.139 ± 0.008	0.149CysTyr: 0.149 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	8.111AspAla: 8.111 ± 0.065	0.414AspCys: 0.414 ± 0.013	3.43AspAsp: 3.43 ± 0.039	3.698AspGlu: 3.698 ± 0.045	1.644AspPhe: 1.644 ± 0.026	6.785AspGly: 6.785 ± 0.068	1.47AspHis: 1.47 ± 0.025	1.893AspIle: 1.893 ± 0.028	1.101AspLys: 1.101 ± 0.024	6.205AspLeu: 6.205 ± 0.062	0.782AspMet: 0.782 ± 0.019	0.894AspAsn: 0.894 ± 0.023	4.353AspPro: 4.353 ± 0.048	1.501AspGln: 1.501 ± 0.024	5.143AspArg: 5.143 ± 0.047	2.449AspSer: 2.449 ± 0.034	3.314AspThr: 3.314 ± 0.038	4.518AspVal: 4.518 ± 0.047	0.959AspTrp: 0.959 ± 0.022	1.081AspTyr: 1.081 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	7.422GluAla: 7.422 ± 0.08	0.367GluCys: 0.367 ± 0.013	2.809GluAsp: 2.809 ± 0.037	3.417GluGlu: 3.417 ± 0.048	1.371GluPhe: 1.371 ± 0.022	4.043GluGly: 4.043 ± 0.037	1.522GluHis: 1.522 ± 0.026	2.136GluIle: 2.136 ± 0.031	1.321GluLys: 1.321 ± 0.03	6.53GluLeu: 6.53 ± 0.07	0.879GluMet: 0.879 ± 0.018	0.967GluAsn: 0.967 ± 0.02	3.315GluPro: 3.315 ± 0.037	2.309GluGln: 2.309 ± 0.036	5.542GluArg: 5.542 ± 0.045	2.419GluSer: 2.419 ± 0.031	2.889GluThr: 2.889 ± 0.032	4.237GluVal: 4.237 ± 0.045	0.727GluTrp: 0.727 ± 0.017	1.043GluTyr: 1.043 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.78PheAla: 3.78 ± 0.04	0.279PheCys: 0.279 ± 0.011	1.986PheAsp: 1.986 ± 0.032	1.399PheGlu: 1.399 ± 0.026	0.936PhePhe: 0.936 ± 0.022	2.989PheGly: 2.989 ± 0.037	0.657PheHis: 0.657 ± 0.017	0.691PheIle: 0.691 ± 0.02	0.489PheLys: 0.489 ± 0.017	2.6PheLeu: 2.6 ± 0.039	0.408PheMet: 0.408 ± 0.012	0.543PheAsn: 0.543 ± 0.016	1.434PhePro: 1.434 ± 0.027	0.666PheGln: 0.666 ± 0.016	1.91PheArg: 1.91 ± 0.028	1.439PheSer: 1.439 ± 0.027	2.008PheThr: 2.008 ± 0.029	2.135PheVal: 2.135 ± 0.035	0.378PheTrp: 0.378 ± 0.012	0.537PheTyr: 0.537 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.718GlyAla: 11.718 ± 0.087	0.87GlyCys: 0.87 ± 0.019	5.28GlyAsp: 5.28 ± 0.052	5.1GlyGlu: 5.1 ± 0.049	2.862GlyPhe: 2.862 ± 0.039	9.343GlyGly: 9.343 ± 0.094	2.477GlyHis: 2.477 ± 0.032	3.316GlyIle: 3.316 ± 0.04	2.524GlyLys: 2.524 ± 0.046	9.513GlyLeu: 9.513 ± 0.073	2.001GlyMet: 2.001 ± 0.031	1.691GlyAsn: 1.691 ± 0.03	5.849GlyPro: 5.849 ± 0.061	2.871GlyGln: 2.871 ± 0.036	8.225GlyArg: 8.225 ± 0.057	5.324GlySer: 5.324 ± 0.056	6.713GlyThr: 6.713 ± 0.058	7.318GlyVal: 7.318 ± 0.058	1.629GlyTrp: 1.629 ± 0.029	2.205GlyTyr: 2.205 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.825HisAla: 2.825 ± 0.033	0.256HisCys: 0.256 ± 0.011	1.385HisAsp: 1.385 ± 0.027	1.18HisGlu: 1.18 ± 0.021	0.703HisPhe: 0.703 ± 0.02	2.524HisGly: 2.524 ± 0.03	0.803HisHis: 0.803 ± 0.02	0.705HisIle: 0.705 ± 0.015	0.347HisLys: 0.347 ± 0.014	2.574HisLeu: 2.574 ± 0.035	0.34HisMet: 0.34 ± 0.012	0.361HisAsn: 0.361 ± 0.012	1.92HisPro: 1.92 ± 0.03	0.722HisGln: 0.722 ± 0.02	2.31HisArg: 2.31 ± 0.032	1.057HisSer: 1.057 ± 0.021	1.466HisThr: 1.466 ± 0.025	1.651HisVal: 1.651 ± 0.025	0.379HisTrp: 0.379 ± 0.012	0.486HisTyr: 0.486 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.456IleAla: 4.456 ± 0.043	0.286IleCys: 0.286 ± 0.009	1.991IleAsp: 1.991 ± 0.031	1.834IleGlu: 1.834 ± 0.029	0.658IlePhe: 0.658 ± 0.016	3.427IleGly: 3.427 ± 0.041	0.58IleHis: 0.58 ± 0.014	0.81IleIle: 0.81 ± 0.021	0.686IleLys: 0.686 ± 0.019	2.2IleLeu: 2.2 ± 0.031	0.398IleMet: 0.398 ± 0.015	0.633IleAsn: 0.633 ± 0.015	1.662IlePro: 1.662 ± 0.025	0.656IleGln: 0.656 ± 0.015	2.118IleArg: 2.118 ± 0.033	1.572IleSer: 1.572 ± 0.028	2.109IleThr: 2.109 ± 0.032	2.471IleVal: 2.471 ± 0.033	0.342IleTrp: 0.342 ± 0.014	0.479IleTyr: 0.479 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.8LysAla: 2.8 ± 0.046	0.119LysCys: 0.119 ± 0.006	1.336LysAsp: 1.336 ± 0.027	1.169LysGlu: 1.169 ± 0.026	0.447LysPhe: 0.447 ± 0.015	1.794LysGly: 1.794 ± 0.032	0.414LysHis: 0.414 ± 0.014	0.855LysIle: 0.855 ± 0.022	0.912LysLys: 0.912 ± 0.033	1.955LysLeu: 1.955 ± 0.034	0.38LysMet: 0.38 ± 0.014	0.521LysAsn: 0.521 ± 0.017	1.317LysPro: 1.317 ± 0.027	0.685LysGln: 0.685 ± 0.018	1.389LysArg: 1.389 ± 0.029	1.102LysSer: 1.102 ± 0.025	1.214LysThr: 1.214 ± 0.028	1.743LysVal: 1.743 ± 0.033	0.26LysTrp: 0.26 ± 0.01	0.419LysTyr: 0.419 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.317LeuAla: 15.317 ± 0.115	0.933LeuCys: 0.933 ± 0.02	6.65LeuAsp: 6.65 ± 0.057	4.602LeuGlu: 4.602 ± 0.055	2.713LeuPhe: 2.713 ± 0.038	9.125LeuGly: 9.125 ± 0.063	2.457LeuHis: 2.457 ± 0.036	2.982LeuIle: 2.982 ± 0.037	1.951LeuLys: 1.951 ± 0.034	11.404LeuLeu: 11.404 ± 0.099	1.621LeuMet: 1.621 ± 0.024	1.638LeuAsn: 1.638 ± 0.027	6.826LeuPro: 6.826 ± 0.058	2.165LeuGln: 2.165 ± 0.036	9.011LeuArg: 9.011 ± 0.073	5.359LeuSer: 5.359 ± 0.052	6.878LeuThr: 6.878 ± 0.06	8.479LeuVal: 8.479 ± 0.067	1.269LeuTrp: 1.269 ± 0.026	1.886LeuTyr: 1.886 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.29MetAla: 2.29 ± 0.029	0.141MetCys: 0.141 ± 0.007	0.932MetAsp: 0.932 ± 0.019	0.74MetGlu: 0.74 ± 0.019	0.427MetPhe: 0.427 ± 0.014	1.309MetGly: 1.309 ± 0.027	0.35MetHis: 0.35 ± 0.012	0.622MetIle: 0.622 ± 0.015	0.409MetLys: 0.409 ± 0.013	1.647MetLeu: 1.647 ± 0.027	0.293MetMet: 0.293 ± 0.011	0.416MetAsn: 0.416 ± 0.013	1.124MetPro: 1.124 ± 0.023	0.461MetGln: 0.461 ± 0.012	1.376MetArg: 1.376 ± 0.024	1.24MetSer: 1.24 ± 0.02	1.425MetThr: 1.425 ± 0.025	1.253MetVal: 1.253 ± 0.021	0.243MetTrp: 0.243 ± 0.01	0.342MetTyr: 0.342 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.156AsnAla: 2.156 ± 0.029	0.162AsnCys: 0.162 ± 0.007	0.88AsnAsp: 0.88 ± 0.019	0.754AsnGlu: 0.754 ± 0.018	0.468AsnPhe: 0.468 ± 0.014	1.881AsnGly: 1.881 ± 0.026	0.38AsnHis: 0.38 ± 0.013	0.619AsnIle: 0.619 ± 0.018	0.4AsnLys: 0.4 ± 0.012	1.58AsnLeu: 1.58 ± 0.024	0.277AsnMet: 0.277 ± 0.012	0.4AsnAsn: 0.4 ± 0.015	1.239AsnPro: 1.239 ± 0.022	0.482AsnGln: 0.482 ± 0.014	1.277AsnArg: 1.277 ± 0.026	0.871AsnSer: 0.871 ± 0.019	1.06AsnThr: 1.06 ± 0.023	1.26AsnVal: 1.26 ± 0.025	0.281AsnTrp: 0.281 ± 0.014	0.397AsnTyr: 0.397 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.522ProAla: 9.522 ± 0.086	0.379ProCys: 0.379 ± 0.012	4.787ProAsp: 4.787 ± 0.053	4.226ProGlu: 4.226 ± 0.042	1.539ProPhe: 1.539 ± 0.028	7.311ProGly: 7.311 ± 0.064	1.498ProHis: 1.498 ± 0.024	1.086ProIle: 1.086 ± 0.019	1.155ProLys: 1.155 ± 0.023	5.488ProLeu: 5.488 ± 0.053	0.983ProMet: 0.983 ± 0.021	0.859ProAsn: 0.859 ± 0.019	3.851ProPro: 3.851 ± 0.055	1.874ProGln: 1.874 ± 0.042	4.213ProArg: 4.213 ± 0.044	3.129ProSer: 3.129 ± 0.046	3.03ProThr: 3.03 ± 0.047	5.606ProVal: 5.606 ± 0.049	0.899ProTrp: 0.899 ± 0.024	1.469ProTyr: 1.469 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.871GlnAla: 3.871 ± 0.051	0.191GlnCys: 0.191 ± 0.008	1.524GlnAsp: 1.524 ± 0.022	1.509GlnGlu: 1.509 ± 0.027	0.662GlnPhe: 0.662 ± 0.018	2.319GlnGly: 2.319 ± 0.033	0.759GlnHis: 0.759 ± 0.017	0.977GlnIle: 0.977 ± 0.017	0.581GlnLys: 0.581 ± 0.018	3.138GlnLeu: 3.138 ± 0.037	0.478GlnMet: 0.478 ± 0.016	0.471GlnAsn: 0.471 ± 0.015	1.808GlnPro: 1.808 ± 0.041	1.415GlnGln: 1.415 ± 0.046	2.522GlnArg: 2.522 ± 0.035	1.245GlnSer: 1.245 ± 0.024	1.263GlnThr: 1.263 ± 0.023	2.354GlnVal: 2.354 ± 0.035	0.493GlnTrp: 0.493 ± 0.013	0.587GlnTyr: 0.587 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.383ArgAla: 10.383 ± 0.085	0.658ArgCys: 0.658 ± 0.017	4.446ArgAsp: 4.446 ± 0.044	4.745ArgGlu: 4.745 ± 0.049	2.379ArgPhe: 2.379 ± 0.032	6.0ArgGly: 6.0 ± 0.049	2.299ArgHis: 2.299 ± 0.027	3.135ArgIle: 3.135 ± 0.033	1.671ArgLys: 1.671 ± 0.032	9.044ArgLeu: 9.044 ± 0.076	1.776ArgMet: 1.776 ± 0.026	1.371ArgAsn: 1.371 ± 0.023	5.352ArgPro: 5.352 ± 0.057	2.497ArgGln: 2.497 ± 0.03	7.861ArgArg: 7.861 ± 0.068	4.138ArgSer: 4.138 ± 0.044	5.95ArgThr: 5.95 ± 0.059	5.756ArgVal: 5.756 ± 0.053	1.383ArgTrp: 1.383 ± 0.026	1.878ArgTyr: 1.878 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.769SerAla: 6.769 ± 0.052	0.431SerCys: 0.431 ± 0.013	2.486SerAsp: 2.486 ± 0.034	2.216SerGlu: 2.216 ± 0.032	1.526SerPhe: 1.526 ± 0.024	6.053SerGly: 6.053 ± 0.062	1.024SerHis: 1.024 ± 0.021	1.244SerIle: 1.244 ± 0.028	0.986SerLys: 0.986 ± 0.025	4.758SerLeu: 4.758 ± 0.045	1.002SerMet: 1.002 ± 0.019	0.78SerAsn: 0.78 ± 0.019	3.207SerPro: 3.207 ± 0.037	1.206SerGln: 1.206 ± 0.021	3.7SerArg: 3.7 ± 0.038	2.642SerSer: 2.642 ± 0.045	2.78SerThr: 2.78 ± 0.036	4.153SerVal: 4.153 ± 0.043	0.866SerTrp: 0.866 ± 0.019	1.141SerTyr: 1.141 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.467ThrAla: 9.467 ± 0.067	0.441ThrCys: 0.441 ± 0.013	3.658ThrAsp: 3.658 ± 0.042	3.135ThrGlu: 3.135 ± 0.038	1.62ThrPhe: 1.62 ± 0.029	7.173ThrGly: 7.173 ± 0.058	1.197ThrHis: 1.197 ± 0.022	1.5ThrIle: 1.5 ± 0.028	1.049ThrLys: 1.049 ± 0.025	5.609ThrLeu: 5.609 ± 0.052	0.889ThrMet: 0.889 ± 0.019	0.896ThrAsn: 0.896 ± 0.021	4.067ThrPro: 4.067 ± 0.043	1.277ThrGln: 1.277 ± 0.024	3.942ThrArg: 3.942 ± 0.038	2.987ThrSer: 2.987 ± 0.032	3.617ThrThr: 3.617 ± 0.048	5.979ThrVal: 5.979 ± 0.055	0.873ThrTrp: 0.873 ± 0.02	1.345ThrTyr: 1.345 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	10.353ValAla: 10.353 ± 0.08	0.793ValCys: 0.793 ± 0.018	4.581ValAsp: 4.581 ± 0.045	4.395ValGlu: 4.395 ± 0.045	2.364ValPhe: 2.364 ± 0.033	6.284ValGly: 6.284 ± 0.051	1.991ValHis: 1.991 ± 0.029	2.679ValIle: 2.679 ± 0.036	1.597ValLys: 1.597 ± 0.029	9.564ValLeu: 9.564 ± 0.079	1.379ValMet: 1.379 ± 0.022	1.616ValAsn: 1.616 ± 0.027	5.478ValPro: 5.478 ± 0.053	2.134ValGln: 2.134 ± 0.028	7.445ValArg: 7.445 ± 0.059	4.07ValSer: 4.07 ± 0.04	5.468ValThr: 5.468 ± 0.051	7.413ValVal: 7.413 ± 0.068	1.102ValTrp: 1.102 ± 0.022	1.484ValTyr: 1.484 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.635TrpAla: 1.635 ± 0.024	0.165TrpCys: 0.165 ± 0.008	0.806TrpAsp: 0.806 ± 0.022	0.698TrpGlu: 0.698 ± 0.016	0.51TrpPhe: 0.51 ± 0.014	0.99TrpGly: 0.99 ± 0.023	0.398TrpHis: 0.398 ± 0.012	0.499TrpIle: 0.499 ± 0.015	0.37TrpLys: 0.37 ± 0.013	1.745TrpLeu: 1.745 ± 0.027	0.295TrpMet: 0.295 ± 0.01	0.394TrpAsn: 0.394 ± 0.015	0.784TrpPro: 0.784 ± 0.016	0.665TrpGln: 0.665 ± 0.018	1.324TrpArg: 1.324 ± 0.027	0.925TrpSer: 0.925 ± 0.019	0.97TrpThr: 0.97 ± 0.017	0.92TrpVal: 0.92 ± 0.019	0.322TrpTrp: 0.322 ± 0.014	0.335TrpTyr: 0.335 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.82TyrAla: 2.82 ± 0.038	0.187TyrCys: 0.187 ± 0.009	1.413TyrAsp: 1.413 ± 0.033	1.257TyrGlu: 1.257 ± 0.022	0.636TyrPhe: 0.636 ± 0.018	2.348TyrGly: 2.348 ± 0.035	0.413TyrHis: 0.413 ± 0.014	0.451TyrIle: 0.451 ± 0.015	0.352TyrLys: 0.352 ± 0.014	2.078TyrLeu: 2.078 ± 0.028	0.248TyrMet: 0.248 ± 0.01	0.397TyrAsn: 0.397 ± 0.013	1.046TyrPro: 1.046 ± 0.02	0.554TyrGln: 0.554 ± 0.015	1.906TyrArg: 1.906 ± 0.03	0.855TyrSer: 0.855 ± 0.018	1.13TyrThr: 1.13 ± 0.02	1.701TyrVal: 1.701 ± 0.03	0.349TyrTrp: 0.349 ± 0.012	0.417TyrTyr: 0.417 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7549 proteins (2594322 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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