Salinihabitans flavidus
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3934 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H8T3X1|A0A1H8T3X1_9RHOB Uncharacterized protein OS=Salinihabitans flavidus OX=569882 GN=SAMN04490248_11421 PE=4 SV=1
MM1 pKa = 7.89 ILIALVAGLLVGGSYY16 pKa = 11.19 LLYY19 pKa = 10.39 LQFDD23 pKa = 4.45 RR24 pKa = 11.84 GCADD28 pKa = 3.61 ADD30 pKa = 3.93 CFVEE34 pKa = 5.59 AVQACEE40 pKa = 4.03 PEE42 pKa = 4.32 TFRR45 pKa = 11.84 TQSPAGTGAEE55 pKa = 3.79 GVYY58 pKa = 10.01 RR59 pKa = 11.84 VVGPAGPACEE69 pKa = 3.94 ISFEE73 pKa = 4.21 YY74 pKa = 10.74 SEE76 pKa = 4.6 NPNPTFVDD84 pKa = 3.64 KK85 pKa = 10.79 PVTFVLSPDD94 pKa = 3.47 EE95 pKa = 4.47 ASEE98 pKa = 4.11 DD99 pKa = 3.94 TVLGALEE106 pKa = 4.15 ACMTGRR112 pKa = 11.84 TGPYY116 pKa = 9.06 QCGGPLFDD124 pKa = 4.78 QLAGSAPAYY133 pKa = 10.44 ASLEE137 pKa = 4.12 YY138 pKa = 10.91 GGDD141 pKa = 3.76 GPLPCGDD148 pKa = 3.75 PVEE151 pKa = 4.86 GAGEE155 pKa = 4.1 PLYY158 pKa = 10.21 PMPKK162 pKa = 9.47 DD163 pKa = 3.83 GQWGYY168 pKa = 7.36 VTRR171 pKa = 11.84 DD172 pKa = 3.84 GEE174 pKa = 4.39 WQIQPQWDD182 pKa = 3.47 RR183 pKa = 11.84 AMDD186 pKa = 3.68 FHH188 pKa = 7.39 EE189 pKa = 4.82 GRR191 pKa = 11.84 AIVGGKK197 pKa = 8.37 NAWGIIDD204 pKa = 4.17 RR205 pKa = 11.84 DD206 pKa = 3.86 GEE208 pKa = 4.18 EE209 pKa = 4.3 IIAPEE214 pKa = 4.2 HH215 pKa = 6.42 ASDD218 pKa = 5.1 VDD220 pKa = 3.72 GQPPFTPYY228 pKa = 10.61 SEE230 pKa = 4.45 GCTVANIFTDD240 pKa = 4.04 TSQPAFFLDD249 pKa = 3.98 RR250 pKa = 11.84 DD251 pKa = 3.81 GKK253 pKa = 10.93 AYY255 pKa = 9.5 WRR257 pKa = 11.84 DD258 pKa = 3.46 EE259 pKa = 4.16 RR260 pKa = 11.84 PEE262 pKa = 3.84 ALAALEE268 pKa = 4.34 IKK270 pKa = 10.85 NFGNFSEE277 pKa = 4.41 GLAWFWDD284 pKa = 3.47 GDD286 pKa = 4.18 IIEE289 pKa = 5.13 PSYY292 pKa = 11.47 GWIDD296 pKa = 3.35 ASGSVAIEE304 pKa = 4.09 PEE306 pKa = 4.23 FVDD309 pKa = 5.0 AGDD312 pKa = 3.83 FSGGLAPASSRR323 pKa = 11.84 EE324 pKa = 4.03 GQAGFISPDD333 pKa = 2.92 GRR335 pKa = 11.84 LVLPRR340 pKa = 11.84 KK341 pKa = 6.46 WTLHH345 pKa = 4.93 NAQPFSEE352 pKa = 4.6 GLAQVWTDD360 pKa = 3.57 AFDD363 pKa = 3.28 IAYY366 pKa = 9.61 MNDD369 pKa = 3.06 SDD371 pKa = 5.22 FAFHH375 pKa = 7.74 DD376 pKa = 3.9 VTFPAADD383 pKa = 3.47 GSDD386 pKa = 3.45 PVRR389 pKa = 11.84 GQIEE393 pKa = 4.17 AAGPFHH399 pKa = 7.74 DD400 pKa = 4.62 GLAPVVAVYY409 pKa = 10.84 DD410 pKa = 3.8 GTTEE414 pKa = 4.02 FAYY417 pKa = 10.09 MNQQGEE423 pKa = 4.6 VAFVPDD429 pKa = 4.17 RR430 pKa = 11.84 LDD432 pKa = 5.22 GIALCDD438 pKa = 3.28 ARR440 pKa = 11.84 LRR442 pKa = 11.84 PEE444 pKa = 3.88 FHH446 pKa = 6.67 NGLVRR451 pKa = 11.84 LVVADD456 pKa = 4.7 DD457 pKa = 4.56 GEE459 pKa = 4.78 SCGDD463 pKa = 3.37 EE464 pKa = 4.42 SYY466 pKa = 11.71 LDD468 pKa = 3.68 GAPAYY473 pKa = 9.89 DD474 pKa = 3.66 AAHH477 pKa = 6.23 YY478 pKa = 10.27 LYY480 pKa = 10.66 LDD482 pKa = 3.5 TQGRR486 pKa = 11.84 VAMQQVKK493 pKa = 10.21
Molecular weight: 53.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.948
IPC_protein 3.961
Toseland 3.757
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.897
Nozaki 4.05
DTASelect 4.279
Thurlkill 3.795
EMBOSS 3.872
Sillero 4.088
Patrickios 1.481
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A1H8SH60|A0A1H8SH60_9RHOB Riboflavin biosynthesis protein OS=Salinihabitans flavidus OX=569882 GN=SAMN04490248_11224 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3934
0
3934
1213260
28
2144
308.4
33.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.146 ± 0.052
0.896 ± 0.011
6.053 ± 0.034
6.192 ± 0.038
3.686 ± 0.025
8.86 ± 0.044
2.149 ± 0.018
5.122 ± 0.03
2.818 ± 0.032
10.023 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.816 ± 0.018
2.437 ± 0.022
5.15 ± 0.026
3.03 ± 0.021
7.31 ± 0.043
5.043 ± 0.026
5.4 ± 0.025
7.291 ± 0.035
1.412 ± 0.017
2.165 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here