Amnibacterium flavum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Amnibacterium

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V1HSZ4|A0A2V1HSZ4_9MICO Glucose-6-phosphate dehydrogenase OS=Amnibacterium flavum OX=2173173 GN=DDQ50_10025 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 5.1PCILPSTGFDD12 pKa = 3.37PSLLLGLTAVAILGGGLSVWAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84SKK39 pKa = 10.86RR40 pKa = 11.84SGVALAVPFLVLLLLAGSQVTPPAASAAVACPPAPAGAPATGGGTTAPVTFEE92 pKa = 4.75PGSAGIGDD100 pKa = 4.59PYY102 pKa = 11.24YY103 pKa = 10.22PLDD106 pKa = 3.92GNGGYY111 pKa = 10.15DD112 pKa = 3.46VQNYY116 pKa = 10.19DD117 pKa = 4.53LDD119 pKa = 4.18LAYY122 pKa = 10.6DD123 pKa = 3.83PATDD127 pKa = 3.75VLAGTATIDD136 pKa = 4.58LVATQNLSAFNLDD149 pKa = 3.11FDD151 pKa = 4.18TRR153 pKa = 11.84DD154 pKa = 3.14AAGADD159 pKa = 3.84SIVIGDD165 pKa = 3.63VRR167 pKa = 11.84VDD169 pKa = 3.56GASTAFSLASTQISLVTAQPVGDD192 pKa = 4.4GSPDD196 pKa = 3.21AVAEE200 pKa = 4.26PPRR203 pKa = 11.84TEE205 pKa = 4.23LTVMPATGILSGTEE219 pKa = 3.59LSVEE223 pKa = 3.62VDD225 pKa = 3.49YY226 pKa = 11.72AGVPITIDD234 pKa = 3.31DD235 pKa = 3.93AFGLGGVLHH244 pKa = 6.68TPDD247 pKa = 2.92GMVPVGQPRR256 pKa = 11.84AAAVWFPSNDD266 pKa = 3.2HH267 pKa = 6.81PADD270 pKa = 3.67KK271 pKa = 10.26ATLTMSMTVPIGLDD285 pKa = 3.28VIGNGVLASTVDD297 pKa = 3.58NGPTSTWTYY306 pKa = 10.86VMDD309 pKa = 4.54RR310 pKa = 11.84PMATYY315 pKa = 10.4LATAVVGDD323 pKa = 4.29YY324 pKa = 10.81DD325 pKa = 4.0VQTTTVDD332 pKa = 3.0GVTYY336 pKa = 10.21RR337 pKa = 11.84DD338 pKa = 3.45AVAQSLYY345 pKa = 10.96SQGTLGADD353 pKa = 2.89ARR355 pKa = 11.84TALDD359 pKa = 4.2LNPDD363 pKa = 4.07VIAYY367 pKa = 9.55LSSIYY372 pKa = 10.49GPYY375 pKa = 10.28PFTEE379 pKa = 4.17SGGIAVDD386 pKa = 5.12LPDD389 pKa = 5.07LGYY392 pKa = 11.25ALEE395 pKa = 4.36NQTRR399 pKa = 11.84PIYY402 pKa = 10.75GFIDD406 pKa = 4.01EE407 pKa = 4.51PTVVHH412 pKa = 6.5EE413 pKa = 5.68LAHH416 pKa = 5.23QWYY419 pKa = 9.07GDD421 pKa = 3.62DD422 pKa = 3.61VAIEE426 pKa = 4.08RR427 pKa = 11.84WSDD430 pKa = 2.35IWLNEE435 pKa = 3.39AFATYY440 pKa = 10.27SEE442 pKa = 4.41WLWSEE447 pKa = 3.98DD448 pKa = 3.39HH449 pKa = 7.06GGDD452 pKa = 3.19TAQQIFDD459 pKa = 3.94DD460 pKa = 4.87TYY462 pKa = 11.72SLDD465 pKa = 3.51ASDD468 pKa = 4.88PFWSVEE474 pKa = 4.02VADD477 pKa = 4.81PGAPRR482 pKa = 11.84LFDD485 pKa = 3.21NATYY489 pKa = 10.82YY490 pKa = 10.43RR491 pKa = 11.84GAMTLHH497 pKa = 7.38ALRR500 pKa = 11.84LEE502 pKa = 4.39VGDD505 pKa = 3.87PVFFGILRR513 pKa = 11.84GWAADD518 pKa = 3.55NAGGNVSTAQFEE530 pKa = 4.87AYY532 pKa = 10.09ASAAAGTDD540 pKa = 3.21LSGFFDD546 pKa = 3.66TWIHH550 pKa = 6.61SSVKK554 pKa = 9.95PP555 pKa = 3.61

Molecular weight: 57.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V1HWS1|A0A2V1HWS1_9MICO GNAT family N-acetyltransferase OS=Amnibacterium flavum OX=2173173 GN=DDQ50_04145 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3308

0

3308

1063717

28

3212

321.6

34.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.001 ± 0.061

0.457 ± 0.011

6.368 ± 0.052

5.598 ± 0.045

3.169 ± 0.027

9.154 ± 0.042

1.787 ± 0.021

4.824 ± 0.032

1.906 ± 0.029

10.155 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.662 ± 0.015

1.999 ± 0.031

5.38 ± 0.036

2.635 ± 0.022

7.143 ± 0.055

6.251 ± 0.036

6.111 ± 0.045

8.948 ± 0.036

1.427 ± 0.015

2.025 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski